Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot

... FEBS 6435 Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase Katy Montin, Carlo Cervellati, Franco Dallocchio and Stefania ... stronginhibitors of 6-phosphogluconate dehydrogenase, and 4-phospho-d-erythro-nate (4PE) and 4-phospho-d-erythronohydroxamate are two of the stron-gest inhibitors of the Trypanosoma brucei ... thanthe substrate per se. To better understand why these analogues have highafﬁnity and to help in rational drug design, we under-took a thermodynamic characterization of substrate and analogue binding...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... remarkable range of receptor selec-tion and function, with some binding a single receptor,some binding multiple receptors, and some binding one receptor with high afﬁnity and others with lowafﬁnity ... elicit their function by binding receptors of the G-protein-cou-pled receptor class, and the N-terminal domain (N-domain) of the receptoris one of the two critical ligand -binding sites. In this ... system, and the ability to ﬁne-tune the enthalpic and entropic components of the binding to the N-domain plays a key role in modu-lating afﬁnity and ligand ⁄ receptor selectivity.Results and DiscussionOn...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... is localized to endosomes and theC terminus (255–448) to the nucleoplasm. To ourknowledge, Osh6p may be the ﬁrst of the OSBPhomologs to be shown to localize to the nucleoplasm and the physiological ... function of all seven OSH gene prod-ucts [10,11]. Those studies provided invaluable in-sights into understanding the function of this family of proteins in yeast and offered guidance to futureresearch. ... limiting membrane of late endosomesinvaginates and buds into the lumen of the organelle, and is responsible for the biosynthetic delivery of lyso-somal hydrolases and the down-regulation of numerousactivated...

Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-speciﬁc terminal uridylyltransferase potx

... nucleotide, able to direct the incorporation of GMP residues into the opposite3¢-end of the mutant RNA.A second mutation of the U6 substrate RNA consisted of the introduction of two additional ... lanes 3 and 8) and showed no detectable activity at and above 200 mMKCl(lanes 4 and 5 and 9 and 10). Therefore, although slightdifferences between the two enzymes were observed inresponse to ... nucleotide. A close examination of the bands inlanes 2–4 of Fig. 2A revealed that concomitant withincreasing length of the UMP tails of the substrate RNA,a decreasing number of distinguishable labeled...

Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot

... Canada;2Department of Molecular Cell Biology,Sylvius Laboratory, Leiden, the Netherlands;3Section of Molecular and Cellular Biology, University of California,Davis, Bodega Bay, CA, USA;4Department of Biological ... [28,29], and the occurrence of both ferritintypes in invertebrates may be the norm. Plants, on the otherhand, are thought to contain a single class of ferritin,restricted to plastids and sharing ... 5¢ end and DNAStrider 1.2 for analysis [38].Structural characterization of artemin and ferritincDNAs and proteins Of the 672 analyzed clones in the Artemia EST library,one artemin and two...

Báo cáo khoa học: Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of monoclonal antibodies directed against the MalK subunit pot

... periplasmic maltose binding protein, MalE, and of the membrane-associated complex, MalFGK2,con-sisting of one copy each of the hydrophobic subunits MalF and MalG and two copies of the nucleotide -binding ... of mAbs 4B3 and 2F9 to their epitopesThe maltose transporter of E. coli and S. typhimurium isinvolved in the regulation of transcription of genes belong-ing to the maltose regulon [26]. This ... isolation and characterization of nine monoclonal antibodies raisedagainst the MalK subunit of the maltose ABC transporter of S. typhimurium that bind to four nonoverlapping linearepitopes. Two epitopes,...

Báo cáo khoa học: "Serial Combination of Rules and Statistics: A Case Study in Czech Tagging" potx

... form of a restarting automaton with dele-tion (Pl´atek et al., 1995), that is, each rule canbe thought of as a ﬁnite-state automaton startingfrom the beginning of the sentence and passing to the ... is demanding and requires a good deal of very subtle linguisticexpertise and skills if full disambiguation also of “difﬁcult” texts is to be performed.1.3 System CombinationCombination of (manual) ... power of a ﬁnite state automa-ton, but the system as a whole has (even morethan) a context-free power.2.2 The Rules and Their ImplementationThe system of hand-written rules for Czech has atwofold...

Báo cáo khoa học: Identiﬁcation of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx

... a result of an improper ori-entation of the cofactor that impairs catalysis ormalate binding, as was described for site-directedmutants of Ascaris suum NAD-ME and for ATP binding to human ... & Wagner-Dobler I (2010) Char-acterization of mleR, a positive regulator of malolacticfermentation and part of the acid tolerance response inStreptococcus mutans . BMC Microbiol 10, 58.10 ... ﬁrstenzyme to be characterized. This enzymatic reactionconverts OAA (derived from citrate) into pyruvate inthe presence of divalent metals and in the absence of nicotinamide cofactors [7]. Noteworthy,...

Báo cáo khoa học: "Large Scale Collocation Data and Their Application to Japanese Word Processor Technology" potx

... cost (b-cost) of a segmentation candidate. By this, the collocation tends to have priority over the ordi- nary word. The standard and initial value of each segment cost is 2, and it is increased ... ority to the word which was used lastly or to the high frequency word. In fact, however, this method sometimes tends to cause inadequate conversion due to the lack of consideration of the ... work to describe the consistency of the concurrence of words [Kobayashi, T. et al.,1992], Making a con- currence dictionary for the specific topic or field, and giving the priority to the...

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modiﬁcation sites and structural requirements for substrate processing and subcellular localization pptx

... amino acids 1–560 and 208–1102were ampliﬁed with 5¢-BamHI and 3¢-NotI overhangs, clonedinto pCR2.1-TOPO and subcloned into a pFastBac1 vector(Invitrogen), modiﬁed by the addition of a C-terminal ... amino acids 1–205, 20–205, 50–205 and 70–205 were ampliﬁed with BamHI and AgeI5¢- and 3¢-over-hangs, ligated into pCR2.1-TOPO and ﬁnally subcloned intothe vector pEGFPN1 (Clontech, EMD Biosciences). ... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing.Tandem afﬁnity puriﬁcation and massspectrometric analysis of USP7N-terminal and C-terminal...

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