Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc

... structure of the hGK ATP binary complex shows the residue con-tacts involved in ATP binding at the active site. Ourﬁndings further support conformational regulation of GK by ATP binding, with possible ... steady-state kinetic analyses are also compatible with conformational con-trol of GK catalytic activity by the binding of (Mg )ATP, with possible implications for kinetic coo-perativity with respect ... 6. The ATP -binding site in the MD simulated model structure of the binary hGK ATP complex and the domain motion induced by ATP binding to the hGK apoenzyme. (A) Close-up view of the ATP- binding...

Báo cáo khoa học: "Mechanical Translation Work at the University of Michigan" potx

... parentheses, at either the beginning or end of the line. It might be possible to standardize on the beginning of the line, and to use the open-parenthesis sign, (, at the left to indicate ... matter with the production of signals suitable for feeding a computer). All of the great mass of technical periodical literature will then be rou- tinely translated into many languages. At that ... intervening years either untranslatable or translatable only with great extra difficulty. It is therefore suggested that this standardization be performed now, so that all technical literature published...

Báo cáo khoa học: "Machine Translation Development at the University of Washington" potx

... Department, and its construction under the supervision of Prof. Hill. During my research, I developed linguistic solutions for the identification by machine of grammatical categories, of both predictable ... machine translation process. It is for the purpose of testing the fundamental engineering feasibility of these linguistic solu- tions that the pilot model was planned. Along with these researches ... of compounding in the Russian language and elaborated proposals for the economical dissection of compounds by machine. The other developed into an exhaustive analysis of MT form classes of...

Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx

... 0.0001). Of the 21 individuals with the Trp316Sermutation, 20 had the )1CﬁA mutation. On the other hand, of the 16 individuals with the Cys306Gly mutation, only onehad the )1CﬁA mutation, strongly ... hypothesis that the effect of the Trp316Ser polymorphism was due to its non-randomassociation with the )1CﬁA mutation and that the effect of the Cys306Gly is independent, which is perhaps mediatedby ... the binding for crude hepaticnuclear extracts and puriﬁed TFIID. These results suggestthat the substitution of C with A at the b2GPI transcriptionalinitiation site is a causative mutation...

Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... packing constraints of the environment. The lar-ger and wider the features of the ligands that compete with the occluding loop for binding to the active site, the farther away the occluding loop ... [20].Although the structures of the mature native form of cathepsin B clearly exposed the relevance of the occluding loop for the exopeptidase activity [11], theydo not explain the mechanisms of endopeptidase ... structures of human [22]and rat procathepsins B [23]. They revealed that, in the zymogen form, the propeptide rather than the occluding loop ﬁlls the active site cleft. It was shownthat the single...

Tài liệu Báo cáo khoa học: Branched N-glycans regulate the biological functions of integrins and cadherins doc

... I-likedomain of b1or b3[68] contribute to the regulation of integrin function. Therefore, we speculate that modiﬁ-cation of particular sites, which are involved in regula-tion of the conformation of ... shortcytoplasmic tail (except for b4). The N-terminaldomains of the a- and b-subunits associate to form the integrin headpiece, which contains the extracellularmatrix (ECM) binding site. The C-terminal ... suspension for 60 min to reduce the detachment-induced activation. Cellswere then replated on dishes coated with LN5 (5 nM) for the indicated times. The cell lysates were blotted with antibody...

Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebraﬁsh pptx

... change the preference of site utilization; and (c) promotion of the dissociation of Chordin cysteine-rich (CR)-containingfragments from the ligand. They suggest that the ﬁrst of these three is the ... toward the end of gastrulation, the accumulation of transcriptsaround the blastopore and expression in the ectodermﬂanking the anterior neural plate is detected. mini ﬁn(mfn), the mutant for ... dorsal–ventral patterning, The role of twisted gastrulationis not clearly understood because there are reportson its functions in opposite directions in the BMPpathway. The function of Tsg...

Tài liệu Báo cáo khoa học: Rotary F1-ATPase Is the C-terminus of subunit c ﬁxed or mobile? docx

... Snapshots of c conformation during the f orcedmolecular dynamics calculated with the torque of 56 pNÆnm. The secondary structure of c is shown for the time of 1 ns (the end of initialequilibration), ... and the left one with a torque of 112 pNÆnm, the arrows show the beginning (t ¼ 1ns)andtheend(t¼23 ns) of the forced rotation. With 56 pNÆnm torque, the relaxation of the system was calculated ... showthat about 14% of the total intensity was located in the acband (Table 2).Molecular dynamics simulations of the rotary mobility of c within the ‘hydrophobic bearing’ at the top of a3b3The...

Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx

... (Tm)(kJÆmol)1)DCp(Tm)(kJÆK)1Æmol)1)pH 7.0SKA 52.3 ± 0.1 281 ± 1 6.1 ± 0.3 – – – – – – SKB – – – 45.14 ± 0.03 377 ± 1 6.0 ± 0.3 – – – SKC – – – – – – 71.2 ± 0.2 201 ± 1 0.8 ± 0.2SKBC – – – 43.52 ± 0.05 320 ± 2 (f) 69.6 ± 0.2 224 ... function for the nativestate, Cp(N); the enthalpies and the heat capacities of the unfolded state, DHUand DCpU, and of the aggregate, DHAand DCpA, all them relative to the native state, ... or at pH 6.0 at any concentration investigated, the thermal unfolding of domain A was accompanied by the time-dependent formation of aggregates of SK. Thisproduces a severe deformation of the...

Tài liệu Báo cáo khoa học: "Negative Polarity Licensing at the Syntax-Semantics Interface" doc

... of the form I- A~, , A: m, B1, , B,. (In sequent representations of this style, the comma represents ® on the left side of the sequent and ~ on the right side.) In our new format, then, the ... node is created for each com- pound term A ® B or A ~ B; and nodes are also created for its subterms A and B. Then, for each node of the form A ~ B, we draw a soft edge in the form of a horizontal ... connect the Uterals The final step in assembling the proof net is to con- nect together the literal nodes at the top of the graph. It is at this stage that unification is applied to the variables...

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