Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc

... Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin Magdalena Sokolowska1, Artur Krezel1, Marcin Dyba1, ... comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal b inding site of human and bovine serum albumins (HSA and BSA,respectively) and short peptide analogues ... modelling of C u(II) and Ni(II)transport by albumins. On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained. Thesedata...

Tài liệu Báo cáo Y học: Transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors potx

... transcription. In summary, the data obtained in this study indicate the conservation of the SRF DNA -binding and dimerizationdomains during evolution and the divergence of the transactivation domain. These ... C-terminal regions did not acti-vate CA promoter and strongly repressed the c-fos promoter,independently of the origin of the N-terminal region. The lack of activity of the A. franciscana C-terminalregion ... promoter, to the absence of binding of SRFcoactivators or both. As the cell lines tested are not of cardiacmuscle origen, they possibly do not contain cardiac-speciﬁcFig. 5. Mapping domains of A....

Tài liệu Báo cáo khoa học: "Which words are hard to recognize? Prosodic, lexical, and disﬂuency factors that increase ASR error rates" ppt

... University{sgwater,jurafsky,manning}@stanford.eduAbstractMany factors are thought to increase the chances of misrecognizing a word in ASR,including low frequency, nearby disﬂuencies, short duration, and being at the start of a turn.However, few of these ... initial repetitions.)3.2.1 Disﬂuency featuresPerhaps the most interesting result in Table 2 isthat the effects of disﬂuencies are highly variable de-pending on the type of disﬂuency and the ... setexhibits similar kinds of variability for many of the features we examined. These differences in ASR be-havior between speakers are particularly interestingconsidering that the system we investigated...

Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations inﬂuence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx

... amyloid from the C110)131 peptide.Role of the NH2-terminal sequence The role of the NH2-terminal sequence of the 124 centered peptides included in this study was analyzed by comparing the ... determination of the peptides by FT-IRspectroscopy in D2O. Spectrograms of solutions containing the same peptides included in Fig. 4 were analyzed in D2O (known to preventintra- and intermolecular ... peptides. In the same way, removing the CONH2-terminus of the peptides resulted in increasedamyloid ﬁbril formation. This increase may be due to adirect inhibitory effect of this nine-amino-acid...

Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... the in uence on d ecorin binding of chemical modi-ﬁcation of Lys and Hyl side chains of collagens and peptides. Derivatizations that eliminate the positive charge of Lys/Hyl eliminate the binding ... of collagens and their CNBr peptides demonstrated that acetylation eliminates their binding to decorin. Lys/Hyl side chains are thereforepresent at, or very near to the binding site( s) and theirpositive ... whereas the b strands lining the inner c oncavity contain s everal charged residues exposedto the solvent. The glycosaminoglycan chain and the N-linked oligosaccharides are on the same side of the molecul...

Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx

... residue and/ or by the binding contribution fromtheGalNAcresidue.Docking the glycopeptide to the antibody the sugar ringhas little contact to the protein surface. Only the N-acetylmethyl group ... struc-ture into the binding cavity of SM3 and the binding epitopeobtained from STD NMR is very good.Comparison with the X-ray and previous NMR structuresAs mentioned above, in the crystal cell the ... 36% of the interaction of the full peptide with the binding cavity of SM3. The sizes of the surfaces were determined using distances between ligandatoms and protein atoms of less than 3 A˚. These...

Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx

... parabutoporin and opistoporin The peptides were prepared by solid-phase synthesis byAnsynth Service B.V. (the Netherlands) using the Fmoc/tert-butyl-based methodology with Rink resin as the solidsupport. ... same family, independent of the continent and region where they live. In this study, the biologicalactivity of parabutoporin and opistoporin 1 is comparedwith the activity of melittin and mastoparan. ... may be less than homologies betweenthese peptides and cysteine-free peptides recently described in the venom of spiders and insects.Parabutoporin and opistoporin 1 were synthesizedchemically...

Tài liệu Báo cáo Y học: Antimicrobial dendrimeric peptides pot

... quantitatively inducederythrocytes aggregations.Proteolytic stability The proteolytic stability of d endrimeric peptides to trypsin and chymotrypsin was determined using peptide and enzyme in ratio of ... respectively. In contrast, the hemolyticactivity of the dendrimeric R4 peptides increased only2.3 -and3 .3-foldfromR4toD2R4 and D4R4 peptides, respectively. Int erestingly, the hemolytic activit y of ... Eachcoupling cycle doubled the branching level of lysyl core and after three cycles, the octa-branching Fmoc-Lys4-Lys2-Lys-DPA-NH-resin was achieved (Fig. 3).Synthesis of DmR4 and DmR8These peptides...

Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx

... (b -site APP cleaving enzyme) at the N-terminus of Ab [4,5] and byan as yet unidentiﬁed c-secretase at the C-terminus of Ab(reviewedin[6]).Ab is the main component of vascularamyloid in Alzheimer’s ... awavelength of 490 nm. Total lactate dehydrogenase wasdetermined by lysing cells in 0.2% Triton X-100 in DMEM/10% fetal bovine serum and measuring the total amount of lactate dehydrogenase in the cell ... resulting in damageto the basement membrane and leading to the eventualdestruction of the cells [12]. The loss of VSMC may result in weakening of the vessel wall, its subsequent rupture and ultimately...

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

... points to changes in the dynamical properties of the local environment of the P-pyridoxylgroup due to changes of the protein conformation inducedby the binding of PEP or the ternary combination.Quenching ... high binding afﬁnity of PEP in the presence of Mn2+[28]. The high afﬁnity of PEP in the presence of Mn2+suggests aspeciﬁc interaction between these two ligands in the enzymeactive site. In the ... are colored yellow, and the C-terminal domainsgreen. The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the...

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