Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... solution. On the other hand, the acceleration of the sliding velocity (and the lower number of stops) of thin ﬁlaments could be related toa diminution o f the load in the actin–myosin interface. The ... remained in the pellet independently of the other components and the ionic strength of the mixture.In the presence o f A TP, the amount of bou nd H MM wasvery similar (average value of 52.5 ± ... FEBS 2004 Eﬀect of titin on actin–myosin activity (Eur. J. Biochem. 271) 4581 Regulation of the actin–myosin interaction by titin Nicolas Niederla¨ nder1, Fabrice Raynaud2, Catherine Astier2and...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... development of the lens and maintenance of the olfactory epithelium [102]. The ﬁrst evidence for a developmental function of HSF4 was provided by population genetic studies wheremutations of the Hsf4 ... by its elevated concentration [18]. The question of the molecular basis behind the dynamic expression pattern of HSF2, and thereby itsactivity, was addressed using mouse spermatogenesis as the ... levels [90]. The signiﬁcance of functional HSF2 in the testis is demonstrated by the phenotype of HSF2 null mice, exhibiting reduced sizes of testis and epididymis, altered morphology of the seminiferous...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membranewith the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax ... MAPKERK1153755PPPp53Fig. 1. Regulation of DNA fragmentation by phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK prevents the activation of caspases and the Bcl-2 family, whereas these ... particular emphasis on the induction of DNA fragmentation, which is one of the most typical characteristics of apoptosis.Regulators of DNA fragmentationOne of the terminal processes of apoptosis is...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... 133–138 at the exposed part of the cathepsin B molecule near the occluding loop(Fig. 2C). The location of the epitope indicates that the binding of 2A2 mAb might change the conforma-tion of the loop, ... of 161.8, 28.2 and 13.2 kDa, respectively. The formation of the cathepsin B ⁄ cystatin C complexwas seen as a shift of the elution peak of cathepsin B(Fig. 6A). The addition of 2A2 mAb to the ... and occurs by a two-step mechanism in which the N-terminus of the inhibitor ﬁrst binds to the enzyme, displacing the occluding loop, followed by the binding of another two loops of the inhibitor...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... caused by a movement of the lip formed by a helix 2 and b strand 5 [18]. Apparently, movement of the active site lip prevented binding of the C-terminalresidues over the active site, or the absence ... new positions. The signiﬁcance of this last observa-tion is that due to the proximity of residues 120–125from each subunit in the centre of the trimer, the sidechains of Val122 of one subunit ... mechanism of regulation that generates the apparently attractive feature of anincrease in both S0.5and the sigmoidity of the satura-tion curve for dCTP in response to the binding of dTTP to the...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... termination of the pr otein s ynthesis.These data a dd to the growing body of informationsupporting a preponderant role of p38 in the regulation of gene expression at the level of mRNA stability. The ... further establish the speciﬁcity of action of RhoGTPases on CTGF/CCN2 expression, we examined the ability of the constitutively active form s of Rho GTPases t oenhance the expression of the ... nd modulate the activity of transcription factorseither via direct physical interactions or by sequesteringcofactors required for their activation [29]. The ﬁnalunderstanding of the underlying...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... as the ATP-unbound and Ca2+-boundE1Ca2+form. In the histogram (Fig. 2A) of the dis-tribution of the height of the projection of the embed-ded molecule above the ﬂat membrane surface, the average ... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy-ses of SERCA were performed with the data obtained in the pres-ence of (A) ... channel. This removes the troponin inhibition of the actin–myosin interaction, and induces muscle contraction. When the musclerelaxes, Ca2+needs to be removed from the cytoplasm by the Ca2+-pump...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... setting, the ability of the F0F1-ATP synthase topump protons out of the matrix represents the onlycomponent opposing the action of an uncoupler. On the basis of a recent study by Giorgio ... changes, as the percentagechange in the steady-state rate of the pathway divided by the percentage change in the enzyme activity caus-ing the ﬂux change. The FCCs for ANT and mostother mitochondrial ... would bestrengthened if the FCC of the ANT is sufﬁcientlyhigher than that of the F0F1-ATP synthase for adeninenucleotide ﬂux rates. The determination of the FCCswas performed by measuring...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... lengthrestrictions of this minireview. The work of S.W. wassupported by the Deutsche Forschungsgemeinschaft(We2843 ⁄ 2-1) and BGAG-Stiftung Walter Hesselbach. The work of S.B. is supported by grants from the ... colonizes the surface area of the gastric mucosa in the human stomach and is one of the most adapted microbial pathogens.  50% of the world’s population carries this bacterium, causingchronic, often ... followed by T-pilus assembly. The assem-bled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular...

Tài liệu Báo cáo khoa học: Upregulation of the a-secretase ADAM10 – risk or reason for hope? docx

... point of intervention within the biosyntheticpathway of ADAM10 is provided by directly interfer-ing with the expression of the gene for ADAM10: the promoter region of the gene for ADAM10 has beencharacterized ... treatment of AD, enhance the transport of ADAM10 to the cellsurface and the non-amylodogenic cleavage of APP[91,92].ADAM10 has also been shown to be regulated by the lipid composition of the plasma ... that the prodomain of ADAM10 acts not only as a transientinhibitor, but also as an internal chaperone in the mat-uration of the enzyme. Accordingly, the viral delivery of furin into the brain of...

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