Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity pdf

... ARTICLE Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity Jay W. Fox1 and Solange ... binding siteson a snake venom metalloproteinase cysteine-richdomain. Arch Biochem Biophys 457, 41–46.J. W. Fox and S. M. T. Serrano Snake venom metalloproteinases and venom complexity FEBS Journal ... secretorycells in the gland and transferred to the rough endo-plasmic reticulum, and then the Golgi apparatus, and ﬁnally transported via secretory granules to the lumenof the venom gland [10]....

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... beencharacterized by small-angle X-ray scattering, UV-absorption, CD and ﬂuorescence spectroscopy to yield the ﬁrst insights into its secondary and tertiary structure. The structural model derived from ... no inﬂuence of disulﬁde bonds onthe near-UV CD spectrum must be taken into account.The relatively low signal intensity can probably beattributed to the compensation of positive and negativesignals ... and reconﬁrms the size exclu-sion chromatography results.The ﬂuorescence spectra have an emission wave-length maximum at 334 nm and refer to a hydrophobicenvironment for most or all tryptophan...

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... structures of Bacillus caldove-lox arginase in complex with substrate and inhibitorsreveal new insights into activation, inhibition and cata-lysis in the arginase superfamily. Structure Fold Des ... 2005)doi:10.1111/j.1742-4658.2005.04874.x To examine the interaction of human arginase II (EC 3.5.3.1) with sub-strate and manganese ions, the His120Asn, His145Asn and Asn149Aspmutations were introduced separately. About 53% and ... wild-type and His145Asn enzymes and 16.2 ± 0.5 · 10)8m for the His120Asn variant. Upon mutation, the chro-matographic behavior, tryptophan ﬂuorescence properties (kmax¼ 338–339 nm) and sensitivity...

Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx

... chunkof errors in their parser is due to non-projectivity.So, there is a need to analyse non-projectivity inHindi for a better insight into such constructions.We would like to say here, that ... 2009.c2009 ACL and AFNLP Insights into Non-projectivity in HindiPrashanth Mannem, Himani Chaudhry, Akshar BharatiLanguage Technologies Research Center,International Institute of Information Technology,Gachibowli, ... non-projectivity and b) in terms of linguis-tic phenomenon giving rise to non-projectivity.While a) gives an idea of the kind of grammar for-malisms and parsing algorithms required to handlenon-projective...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... lM)ofisomaltose. Kato et al. reported that t wo a-glucosidasesfrom A. nidulans, AgdA and AgdB, showed strong trans-glycosylation activity to produce isomaltose from maltose, and they are suggested to ... column, TOYOPEARL Hexyl-650C(Tosoh, Japan) was used, the recovery increased to 50%.Ammonium sulfate was added directly to the dialysed crudeenzyme to adjust it to 70% s aturation and the supernatantwas ... reader. To determine the kineticparameters of wild-type and W402F IPU for p anose,mixtures consisting of 4 lgÆmL)1enzyme and from 43 to 216 mMsubstrate, and 20 lgÆmL)1enzyme and 64 to 480...

Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... whole loops and the two connecting aminoacids at the beginning and the end, were mutated to glycines and, after remodelling back to their side chains in agma-tinase, the whole hydrogen bond network ... alignedwith the sequences of 1CEV and 1RLA and pasted into thestructural alignment. The alignment was then corrected byhand. Since the sequences of the two arginases and agmatinase greately differ ... given to the modeling ofthe active site. The modelled structure was very similar to the template, with respect to the number and arrangementof structural elements (a-helix and b-sheets), and...

Tài liệu Báo cáo khoa học: "Parsing into Discourse Object Descriptions" pptx

... (a) to enable the user to make explicit and implicit cross-references between utterances, e.g. by using pronouns and ellipsis; (b) to allow the user to build commands incrementally; (c) to ... booking to be executed on the background system, and (ii) a meeting to be booked. They are labelled, say B1 and M1, and supplied with their descriptions. Moreover, both B1 and M1 are assigned topical ... grammatical (and lexical) information found in the input. Utterance types are not identified with command types as there is nb one -to- one correspondence between inputs and commands to the background...

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... UTRplasmids and seeded into 35 mm plates as described above.After 16 h of incubation, doxycycline was added to stoptranscription from the tetracycline responsive promoter and total RNA was ... of both tissue-type plasminogen activator and urokinase-type plasminogen activator are controlled byplasminogen activator inhibitor types 1 and 2 (PAI-1 and PAI-2, respectively). One of the enigmatic ... samples and the plates were returned to the incubator for further incubation.In vitro transcription and RNase protection assay and northern hybridizationA cDNA library prepared with 1 lg of total...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... conditions led to the migration of ASF ⁄ SF2 into cytoplamic aggregates corresponding to SGs based on their content in eIF3b, another SG marker (Fig. 4C).Several SG components can assemble into SGs ... transcript and the trans-acting factors(RNA-binding proteins and noncoding regulatoryRNAs) that bind to these sequences. Among the beststudied regulatory sequences, the adenine and uridine-rich ... present study aimed to identify the proteinsassembling with TIAR and to characterize their role inTIAR cytoplasmic functions. We used a tandem afﬁn-ity puriﬁcation (TAP) approach to isolate TIAR-asso-ciated...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... major band and several faster-migra-ting faint bands. Alternative splicing occurs in manygenes, and so DNA fragments were puriﬁed from themajor band and cloned into a sequencing vector. DNAsequences ... lg of the DNASE1 expression vectors and 0.6 lg of the pSV–b-galactosidase vector (Promega),followed by assay of DNase I and b-galactosidase activities,according to a previously described method ... extracts and EMSAThe nuclear extract and probe were prepared as reportedpreviously [33], and the sequence of oligonucleotide 90,51probe corresponds to nucleotides )90 to )51 relative to the...

Xem thêm