... exception that sonicationwas used instead of high pressure homogenization, thepurification was conducted on an A ¨KTAxpress system(GE Healthcare).Crystallization, data collection and structuredeterminationCrystals ... Canyuk B, Focia PJ & Eakin AE (2001) The role foran invariant aspartic acid in hypoxanthine phospho-ribosyltransferases is examined using saturation muta-genesis, functional analysis, and ... The Authors Journal compilation ª 2010 FEBS Structural and functional studies of the humanphosphoribosyltransferase domain containing protein 1Martin Welin1,*, Louise Egeblad2,*, Andreas...