Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... the difference of DWm (termedDelta phi) before and after the addition of ADP(2 mm) to WT and CYPD KO mitochondria, and cal-culated on the basis of steady-state titration data by catr and olgm. ... of inhibition by cyclophilin D was evident in the form of slightly increasedrespiration rates during arsenolysis. However, the modulation of F0F1-ATP synthase by cyclophilin D did not increase the adenine ... results obtained indicate that modulation of F0F1-ATP synthase activity by CYPD comprises an‘in-house’ mechanism regulating matrix adenine nucleotide levels that does not transduce to the extra-mitochondrial...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... intensity indeed denoted the formation of highermolecular mass aggregates, SDS ⁄ PAGE and immuno-blotting were carried out according to the proceduredescribed in Materials and methods. a-Synuclein ... containingMPTP (A and C) and MPP+(B and D) were analysed by SDS ⁄ PAGE(A and B) on 5–15% crosslinked polyacrylamide gel and westernblotting (C and D) . (A, B) Lane M, prestained molecular mass ... patients of idiopathic Parkinson’s disease anddevelop the same therapy-related complications. Post-mortem analysis of brains of patients with MPTP-induced Parkinson’s disease has disclosed importantsimilarities...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... exopeptidase activity of cathepsin B in the presence of 2A2 mAb. Furthermore,2A2 mAb also inhibited cathepsin B endopeptidase activity, as determined by the degradation of DQ-col-lagen IV and BODIPY ... (A), degradation products are visible intracellularly and pericel-lularly (white arrow). Addition of the 2A2 mAb to the assaymedium reduced the degradation of DQ-collagen IV (B). Degrada-tion ... of Abz-GIVRAK(Dnp)-OH (ﬁnalconcentration 1 lm) and 10 lL of 2A2 mAb or NaCl ⁄ Piwere added to a well of a black microtiter plate and thereaction was initiated by adding 85 lL of activated...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at least two independentexperiments.lg of sterol per mg of proteinwild-type ste2 0D cla 4D swe 1D cla 4D swe 1D Ergosterol ... morphology of the strains usedin this study. (A) Morphology of deletionstrains. The indicated strains were grown inYPD to stationary phase. The cells werethen ﬁxed with formaldehyde and examined by ... have increased levels of free sterol. The indicated strains were grown to stationary phase,and then lipids were extracted and separated by TLC. The datashown are from two independent experiments....

Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... of FNR for oxidized Fd decreases by morethan 10-fold on addition of NADP+[60]. Consideringthat molecular modeling indicates that neitherNADP+nor the residues C266, G267 and L268 areadjacent ... amino acid groups introduced by mutations was calculated following the standard radii andvolumes calculated by Tsai et al. [32], assuming a reducedstate of the cysteine. Amino acid hydropathy ... mutants of C266, G267 and L268 and adouble mutant of C266 and L268 were successfullyconstructed and conﬁrmed by DNA sequencing. Thedesign of the mutants was intended to preserve theamino acid character...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... and 14 showthe PCR products obtained using the primer pair T7 and ADC2. Lane 15, 1 kb DNA ladder standard. (B) DNA obtained from arginine decarb-oxylase (ADC)-transformed parasites harvested ... plasmid had almost disappeared(Fig. 3A, lanes 7 and 8). The faint PCR band of  3500 bp detected in Fig. 3A (lane 8), in addition tothe expected 2030 bp PCR product, might be gener-ated by a ... conserved regions of ODC or ADC genes from many organisms, have indi-cated the absence of ODC- and ADC-like nucleotide sequences in the wild-type T. cruzi genome [10,17].As the described results...

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis andhyperphosphorylation of Rb by n-3 and n-6 PUFAsInduction of cyclin D1 is one of the e arliest effects of mitogenic ... of n-3 PUFAs(EPA or DHA) d id not stimulate the basal cyclin D1 genepromoter activity. EPA and DHA also reduced theincreased cyclin D1 activity in response to serum.IL-1b-induced cyclin D1 ... washed in NaCl/Pi/Tween and thenincubated with the indicated primary antibodies. Immuno-blots were developed by using appropriate secondaryhorseradish peroxidase-coupled antibodies and t...

Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt

... emulsiﬁed substrate was added and mixed.Reactions were typically incubated for a period of 30 min at37 °C before the reaction was quenched by the addition of 3.25 mL of methanol ⁄ chloroform ... measurements. Spectra of samplescontaining phosphorylated and dialysed HSL mixedwith bis-ANS were recorded, and spectra of dialysedphosphorylation mixes (including PKA, which pro-vided only minor ... measured by the 2D Quant method(GE Healthcare) and the Bradford method [19]. The latterunderestimated HSL content by a factor of 1.5 relative tothe 2D Quant method. The C-terminally His-tagged...

Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt

... started by the addition of 10 l L of NADPH (0.75 mmﬁnal concentration), and continued for 30 min at 37 °C. Todeterminate the Ca2+-independent activity of NOS(i-mtNOS), 10 mm EDTA was added to ... serial dilutions (50, 5, 0.5 and 0.05 ng),and were normalized by b-actin expression.Nitrite determinationMitochondrial fractions were thawed and suspended in ice-cold distilled water, and immediately ... sepsis-inducedinducible nitric oxide synthase inducible mitochondrial nitric oxide syn-thase isoforms, prevented the impairment of mitochondrial homeostasisunder sepsis, and restored ATP production....

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... second antibody against the extracellular domain of E-cadherin, which did not disrupt cell–cell adhesion,did not induce Erk activation. Second, inhibition of Srcblocked the disruption of cell–cell ... up-regulated upondisruption of E-cadherin-dependent cell–cell adhesion. We explored theunderlying molecular mechanisms and found that blockage of E-cadherin by Decma elicits a signaling pathway downstream ... effect of overexpresseddominant negative p52Shc3Y3F. These results revealed anonredundant and isoform-speciﬁc role for Shc in theErk signaling induced by the E-cadherin blockage.Decma-induced...

Xem thêm