... intracellular free cholesterol through the activity of acyl-CoA:cholesterol acyltransferase, ACAT • Regulation of low density lipoprotein (LDL) receptor-mediated cholesterol uptake Fig Structure of cholesterol ... attached to the D ring Cholesterol can be esterified by acyl-CoA: cholesterol acyltransferase (ACAT) to form cholesterol esters Cholesterol ester has a fatty acid attached at carbon 3, which makes ... majority of cholesterol is found in the plasma membrane, but it is synthesized in the endoplasmic 15 reticulum (ER), where the cholesterol level is low and where changes in cellular cholesterol...
... Gabriele T, Cuter G, Cao P, Bhatt AS, Choe Y, Craik CS, Walker N et al (2005) Hepatocyte growth factor is a preferred in vitro substrate for human hepsin, a membrane-anchored serine protease implicated ... follows The expression vectors encoding HAI-1–NK1, HAI-1–NK1LK2 and HAI-2 were introduced into CHO cells using Superfect transfection reagent (Qiagen, Hilden, Germany) Transfected cells were ... Na2HPO4Æ12H2O, 2.68 mm KCl, 1.47 mm KH2PO4, mm Na3VO4, mm EDTA, 1% Nonidet-P40, 0.5% sodium deoxycholate, lgÆmL)1 FEBS Journal 277 (2010) 4888–4900 ª 2010 The Authors Journal compilation ª 2010...
... Valentino ML, Hirano M, Reichard P & Bianchi V (2006) Mitochondrial DNA depletion and thymidine phosphate pool dynamics in a cellular model of mitochondrial neurogastrointestinal encephalomyopathy J ... thymidine (dThd) to dTMP Two thymidine kinases catalyze this step, the cytosolic TK1 and the mitochondrial TK2 (EC 2.7.1.21 for both TK1 and TK2), encoded by two nuclear genes TK1 is cell-cycle-specific...
... MUPP1 antibodies and constructs This work was supported by the International Max-Planck Research School in Chemical Biology (IMPRS-CB), the Studienstiftung des deutschen Volkes, the Heinrich und...
... lysate Due to the commercial availability of suitable quantities of HeLa nuclear pellets, these were chosen as the starting material for a purification of CDK4 Y17 kinases Purification of C-YES as a ... phosphate gradient (Fig 2C) Fractions 38–49 and a sample of the input were precipitated using deoxycholate and trichloroacetic acid, and the proteins were separated using SDS ⁄ PAGE A major protein ... gradient of 10–500 mm phosphate buffer and 500 lL fractions were collected 2.5 lL of sodium deoxycholate (2%, w ⁄ v) was added to 250 lL samples of selected fractions and the samples were incubated...
... Oncogene 21, 1945–1954 31 Vanhaesebroeck B, Leevers SJ, Ahmadi K, Timms J, Katso R, Driscoll PC, Woscholski R, Parker PJ & Waterfield MD (2001) Synthesis and function of 3-phos- 3644 32 33 34 35 36 ... (2000) MT-21 is a synthetic apoptosis inducer that directly induces cytochrome c release from mitochondria Cancer Res 60, 5214–5222 62 Feng SH & Lo SC (1999) Lipid extract of Mycoplasma penetrans ... in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP J Biol Chem 277, 12343–12350 66 Li Y, Zhu H, Kuppusamy P, Roubaud V, Zweier JL & Trush MA (1998)...
... DNA in the nuclei [11–13] The magnitude of these nuclear changes appears to be relevant to the anchoring of DNA loop domains on the nuclear scaffold or matrix, where the nuclear scaffold ⁄ matrix ... It is presumable that such nucleation of aggregates takes place at AT-rich S ⁄ MAR DNA of an anchor of chromatin loops that tend to transform into ssDNA by unwinding [38] and are potential sites ... microscopy and biochemical properties of polyaminecompacted DNA Biochemistry 26, 6387–6392 Tsang SS, Chow SA & Radding CM (1985) Networks of DNA and RecA protein are intermediates in homologous pairing...
... was via direct interactions of the TBP component of TFIIIB with the TATA sequences and was not anchored via interactions with TFIIIC The stable binding (heparinresistant complex formation) also ... via the TATA sequences (Fig 2B, compare lanes and 4) Prior binding of TFIIIC, which presumably anchored the stable binding of TFIIIB, was important for transcription The deductions from the binding...
... Crystallogr D Biol Crystallogr 56, 1359– 1366 19 Lim, J.H., Yu, Y.G., Han, Y.S., Cho, S., Ahn, B.Y., Kim, S.H & Cho, Y (1997) The crystal structure of an Fe-superoxide dismutase from the hyperthermophile ... with hydrogen peroxide Arch Biochem Biophys 345, 156–159 Lim, J.H., Hwang, K.Y., Choi, J., Lee, D.Y., Ahn, B.Y., Cho, Y., Kim, K.S & Han, Y.S (2001) Mutational effects on thermostable superoxide ... spectrophotometer (Showa Woman’s University, Japan) by Professor F Yamakura (Juntendo University School of Medicine, Japan) and Professor T Matsumoto (Showa Woman’s University) RESULTS Mutation...
... was very fast, being completed within one minute at °C (Fig 4C) Then, as standard conditions, we chose 60 as the preincubation time, 20 000 chromatin per assay, and 20 for the time of binding of ... was shown by sperm chromatin in vitro ([6,13]; this study), and by mitotic phase chromosomes from CHO cells [5] Regulation of the binding of LBR to chromatin by phosphorylation was shown in this ... membrane protein J Cell Biol 135, 1715– 1725 38 Dreger, M., Otto, H., Neubauer, G., Mann, M & Hucho, F (1999) Identification of phosphorylation sites in native laminaassociated polypeptide beta...
... DNA fragmentation in chorion laeve tissue during in vitro incubation The concentration of each antioxidant reagent was determined by preliminary examination using cultured chorion laeve cells ... death of trophoblasts in chorion laeve tissues during the late 6186 K Ohyama et al (Eur J Biochem 268) q FEBS 2001 Fig Effect of antioxidants on DNA fragmentation of chorion laeve tissue during ... that the activation of apoptosis is associated with ROS produced by mitochondria [27], that the generation of ROS in mitochondria was activated by TNFa [28] and that apoptosis could be blocked...
... release Am J Physiol Endocrinol Metab 291, E885–E890 3320 48 Choi KC, Ryu OH, Lee KW, Kim HY, Seo JA, Kim SG, Kim NH, Choi DS, Baik SH & Choi KM (2005) Effect of PPAR-alpha and -gamma agonist on ... visfatin Diabetologia 49, 744–747 Seo JA, Jang ES, Kim BG, Ryu OH, Kim HY, Lee KW, Kim SG, Choi KM, Baik SH, Choi DS et al (2008) Plasma visfatin levels are positively associated with circulating ... by cell death [36,37] However, it has been demonstrated in adipocytes and Chinese Hamster ovary (CHO) cells that visfatin is actively secreted through a nonclassical (nonGolgi ⁄ endoplasmic reticulum...
... requires an efficient supply and delivery of ATP from the mitochondria to perform work and maintain ion homeostasis Ca2+ couples mitochondrial ATP production to demand: Ca2+ release during the ... the endoplasmic reticulum, and the outer mitochondrial membrane Cell Growth Differ 5, 411–417 38 Foyouzi-Youssefi R, Arnaudeau S, Borner C, Kelley WL, Tschopp J, Lew DP, Demaurex N & Krause KH (2000) ... MR (2000) Mitochondria and calcium: from cell signalling to cell death J Physiol 529, 57–68 Bcl-2 and calcium control of autophagy 63 Rizzuto R, Bernardi P & Pozzan T (2000) Mitochondria as all-round...
... 4004 A Talamillo et al (Eur J Biochem 271) concentration [7], control of the mitochondrial dNTP pool [8,9], mitochondrial localization to specific cellular domains [10], or changes in local ATP ... regulatory factors in mitochondrial biogenesis was pioneered by Scarpulla’s group [5] This involves characterization of the promoter regions of mammalian nuclear-encoded mitochondrial genes, and has ... play a significant role in mitochondrial biogenesis, the nuclear respiratory factors (NRFs) and [5,14] These factors are likely to be involved in the integration of mitochondrial biogenesis with other...