isolation amplification and labeling of nucleic acids
Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids
... Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization The nucleic acid is a polymer consists of monomers called nucleotides Each nucleotide composes of a nitrogenous ... .142 Table 4.3 Typical concentration and purity of p185-ssDNA standard 144 xii LIST OF FIGURES Figure 1.1 Basic principle of nucleic acids hybridization and detection on a solid surface ... .1 1.1 Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization .1 1.1.2 Label-free detection of nucleic acids 1.1.2.1 Electrochemical ...
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Báo cáo hóa học: " Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids" pot
... Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids ... versatility of the electrode was tested Cyclic voltammetry was used for the detection of two nucleic acids samples mentioned above and the basic electrochemical behaviour of nucleic acids at the ... suitable tool for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals Methods...
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Báo cáo khoa học: "SenseClusters: Unsupervised Clustering and Labeling of Similar Contexts" potx
... names or newgroups, and the number of contexts associated with each The next column indicates the percentage of the majority class (MAJ.) and count (N) of the total number of contexts for the ... Bisections, which offers nearly the quality of agglomerative clustering at the speed of partitional clustering Labeling Methodology For each discovered cluster, we create a descriptive and a discriminating ... names and uncategorized email Table shows the descriptive and discriminating labels assigned to the 2–way experimental case of American Airlines and Tom Cruise, as well as the 3–way case of George...
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Agricultural Biotechnology and Organic Agriculture: National Organic Standards and Labeling of GM Products pot
... Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 93 The market and welfare effects of labeling are shown to depend on the size of segregation costs under mandatory labeling of GMPs, ... Agricultural Biotech and Organic Agriculture: National Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 91 Figure The effects of mandatory labeling of GM products on organic and conventional ... Agricultural Biotech and Organic Agriculture: National Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 92 Table Market and consumer welfare effects of mandatory labeling of GM products...
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Báo cáo khoa học: "Unsupervised Discrimination and Labeling of Ambiguous Names" ppt
... Clinton and Tony Blair (political leaders), and Mexico and India (countries) We also have inter-category disambiguation such as Bayer, Bank of America, and John Grisham (two companies and an author) ... harmonic mean of precision and recall Precision is the percentage of contexts clustered correctly out of those that were attempted Recall is the percentage of contexts clustered correctly out of the ... ambiguity: 2-way, 3-way, and 4-way In each of the three categories we have 3-4 examples that represent a variety of different degrees of ambiguity We have created several examples of intra-category...
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capillary electrophoresis of nucleic acids, volume i
... Giordano, B., and Landers, J P (2001) Toward effective PCR-based amplification of DNA on microfabricated chips, in Capillary Electrophoresis of Nucleic Acids, Vol (Mitchelson, K R and Cheng, J., ... 5.1.1 Isothermal Strand Displacement Amplification of DNA Strand displacement amplification (SDA) (90,91) and NASBA (92) are isothermal in vitro methods for amplification of a specific DNA sequence ... Electrophoresis of Nucleic Acids, Vol 1: Introduction to the Capillary Electrophoresis of Nucleic Acids Edited by: K R Mitchelson and J Cheng © Humana Press Inc., Totowa, NJ 27 28 Slater, Desruisseaux, and...
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capillary electrophoresis of nucleic acids, volume ii
... H., and Foret, F (1995) Capillary electrophoresis of proteins and nucleic acids Annu Rev Biophys Biomol Struct 24, 579–610 Olivera, B M., Baine, P., and Davidson, N (1964) Electrophoresis of nucleic ... detection of nucleic acid sequences: oligonucleotide assay and sequence-coded separation Nucleic Acids Res 22, 4527–4534 19 Day, D J Speiser, P W., White, P C., and Barany, F (1995) Detection of steroid ... values of LD2 and LS2 (region C), the minimum separation path XC and the migration time tC are reduced as well The 22 Klepárník, Mueller, and Foret Fig Effect of lengths of injection zone and detection...
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Packaging and labeling of apparel and tetiles
... •Union labeling Registered number system •PERMANENT CARE LABELING RULE(1972) Voluntary labeling A Brand labeling the labeling of fabrics with brand name is well established method and is used ... umbrellas and parasol dish clothes, dishtowels and wash clothes •• TEXTILE FIBER PRODUCT IDENTIFICATION SILK REGULATION 1932 Voluntary labeling LABELING CATEGORIES VOLUNTARY LABELING •Brand labeling- ... presence of steam CARE LABELING SYSTEMS International care labeling system Japan care labeling system British care labeling system Canadian care labeling system a International Care Labeling...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...
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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)
... surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone (T), estrogen (E) and ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit ... responsiveness of the promoter and the sequence will be analyzed to identify putative regulatory elements that may mediate these effects 23 MATERIALS AND METHODS 2.1 Extraction and isolation of genomic...
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Isolation, cloning and expression of NAD+ glycohydrolase from neurospora crassa
... Culture 2.2.2 Conidia Culture 32 33 ii CONTENTS 2.3 PAGE Isolation of N crassa Proteins 2.3.1 Isolation of Mycelia Proteins 2.3.2 Isolation of Conidia Proteins 33 33 Column Chromatography 2.4.1 ... Cycling Assay 81 Discussions 4.1 Isolation and Characterization of Conidial NADase from N crassa 83 4.2 Identification of NADase Sequence 85 4.3 Cloning and Expression of Gi|85106032 86 4.4 NAD+ Cycling ... LIST OF FIGURES FIGURE TITLE PAGE 1.1 De novo synthesis and salvage pathway of NAD+ 1.2 Reaction mechanism of NADase and ADP-ribosyl cyclase 1.3 Cycling assay for cADPR 15 1.4 Asexual cycle of...
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Báo cáo sinh học: " Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach" pdf
... sequence C Figure LNA-based and bigenic amplification of beta- and gammaherpesviruses LNA-based and bigenic amplification of beta- and gammaherpesviruses Schematic diagram of the analysis strategy ... (gB), and two thirds of the ORF UL54 (DPOL) (Figure 6B) The nucleotide and amino acid sequence differences of CgueCMV-1.1 and CgueCMV-1.2 were 0.6% and 0.5% (UL56), 20% and 8% (UL55) and 1% and ... 761 761 (UL54; partial) Figure Amplification of an kbp locus of CgueCMV-1.1 and CgueCMV-1.2 Amplification of an kbp locus of CgueCMV-1.1 and CgueCMV-1.2 At the top of the figure, the betaherpesvirus...
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báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx
... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... 10 ul of each of the wash through and eluates from each assembly reaction were amplified in parallel using primers T7-F and T7TER-R 35 cycles of amplification of 1' at 96°C, 30" at 40°C and 1'30" ... nucleic acids; (ii) a small molecule ligand or ligands (for example gluthatione), as a nucleic acid label, and an appropriate receptor or protein fragment which binds the ligand as part of the...
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Báo cáo y học: " Contamination of clinical specimens with MLVencoding nucleic acids: implications for XMRV and other candidate human retroviruses" docx
... sources of contamination in PCR-based studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human blood and tissue samples, and ... claims of MLV-related viruses in prostate cancer and CFS patients Future assessments of the prevalence of XMRV should include more rigorous PCR and phylogenetic tests to exclude the possibility of ... Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology 2010 7:112 Submit your next manuscript to BioMed Central and...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution profile of P III and P IV Chromatofocusing chromatography...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...
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Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx
... carcinoma % Sialic acids in resection margins Table Fluorometric-HPLC and GC-MS analysis of glycoconjugate-bound and free sialic acids isolated from the mucosa of matched resection margin and colorectal ... carcinoma tissue Sialic acids were isolated and analysed by HPLC and GC-MS as described in Materials and methods The proportion of individual sialic acids is expressed as a percentage of the total sialic ... (1989) O-acetylation and de-O-acetylation of sialic acids O-acetylation of sialic acids in the rat liver Golgi apparatus involves an acetyl intermediate and essential histidine and lysine residues–a...
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- nomenclature and properties of carboxylic acids
- acidity and basicity of carboxylic acids
- isolation purification and culturing of orchid mycorrhizal fungi omf
- isolation purification and culturing of omf
- protein digestion and absorption of amino acids and oligopeptides
- it s puzzling structure of nucleic acids
- isolation characterization and differentiation of human embryonic stem cells
- electrochemical detection of nucleic acids
- em tomography and multiplex detection of nucleic acids and protein in situ
- fi cation and detection of nucleic acids puri fi ed from dbs
- chemical shift tensors of nucleic acids theory and experiment
- isolation rna amplification and labeling
- 3 7 8 and 9 for the recognition of nucleic acids
- isolation amplification and transferability of ssr markers
- isolation amplification and labeling
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