isolation purification and culturing of orchid mycorrhizal fungi omf

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...

Ngày tải lên: 11/09/2015, 10:06

... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...

Ngày tải lên: 25/10/2013, 22:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

Ngày tải lên: 20/06/2014, 23:20

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

Ngày tải lên: 07/08/2014, 20:23

... fertility and in the maintenance of stability and biodiversity within plant communities [13] The success of any reforestation programme depends on colonisation of the new woodland stands by mycorrhizae ... Tableau II Number of spores, mycorrhizal frequency and intensity of T articulata at the studied sites Tableau III Diversity and abundance of AM fungal spores in Tetraclinis woodlands Sites Site ... Acaulospora sp 3.3 Relative abundance of common AMF species The number of spores per 100 g of dry soil was above 400 at sites 1, and 7, between 210 and 300 at sites and 6, and Sites Glomus constrictum...

Ngày tải lên: 08/08/2014, 00:22

... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...

Ngày tải lên: 12/08/2014, 01:21

... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...

Ngày tải lên: 12/08/2014, 04:21

... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...

Ngày tải lên: 06/10/2015, 13:05

... surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone (T), estrogen (E) and ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit ... responsiveness of the promoter and the sequence will be analyzed to identify putative regulatory elements that may mediate these effects 23 MATERIALS AND METHODS 2.1 Extraction and isolation of genomic...

Ngày tải lên: 08/11/2015, 17:15

... Culture 2.2.2 Conidia Culture 32 33 ii CONTENTS 2.3 PAGE Isolation of N crassa Proteins 2.3.1 Isolation of Mycelia Proteins 2.3.2 Isolation of Conidia Proteins 33 33 Column Chromatography 2.4.1 ... Cycling Assay 81 Discussions 4.1 Isolation and Characterization of Conidial NADase from N crassa 83 4.2 Identification of NADase Sequence 85 4.3 Cloning and Expression of Gi|85106032 86 4.4 NAD+ Cycling ... LIST OF FIGURES FIGURE TITLE PAGE 1.1 De novo synthesis and salvage pathway of NAD+ 1.2 Reaction mechanism of NADase and ADP-ribosyl cyclase 1.3 Cycling assay for cADPR 15 1.4 Asexual cycle of...

Ngày tải lên: 08/11/2015, 17:15

... Rhizobium and mycorrhizal fungi on growth and nitrogen content of A peregrina, (ii) the growth of the other native species in mixed plantation with A peregrina, and (iii) the dynamic of litter ... Figure Nitrogen and phosphorus contents of leaves (dry matter) and root colonization of Anadenanthera peregrina plants inoculated with rhizobia (BHICB) and mycorrhizal fungi (AMF) 4,8 and 12 months ... 2coarse sand = 57.0%, fine sand = 27.0%, silt = 3%, clay = 13%; and block 3- coarse sand = 56.0%, fine sand = 28%, silt = 4%, clay = 12% and chemical analysis is showed in Table III 2.3 Selection of...

Ngày tải lên: 08/08/2014, 01:22

... regeneration potential and a number of clones of the hybrid can be considered as the most successful representatives of the genus in terms of growth rate, regeneration and the establishment of new shoots ... parents The effect of nitrogen on mycorrhizal colonisation was not significant The trend of the frequency of mycorrhizal colonisation (F) was similar to that of the M values and is not shown here ... summary of the effect of mycorrhizal inoculation on the N content with different combinations of clones and soil nitrogen level Mycorrhizal inoculation had no effect on the production and the...

Ngày tải lên: 11/08/2014, 11:20

... Preparation of samples Chloramphenicol and cycloheximide were used as specific growth inhibitors of bacteria and fungi, respectively (Anderson and Domsch, 1975; Newell et al., 1983; Fuhrman and McManus, ... of the resulting N2O concentration (Weiss and Price, 1980; Fogg and Gerrand, 1991) - 69 - Results Characteristics of fungal denitrification in sediments and soils Figure shows the behaviors of ... in the Table Potentials and contributions of bacterial and fungal denitrifications in tea soils, the submerged litter and sediment of an irrigation pond, and the sediment of a tidal river at in...

Ngày tải lên: 05/09/2013, 08:40

... decrease of penetration capacity and purification efficiency during continuous use of the facility was investigated The facility consisted of two tanks In one of the tanks, continuous use of the ... 45 Upper layer Lower layer of weir (cm) (cm) (cm) Akadama sand (5) Pit sand (40) Pit sand (45) Journal of Water and Environment Technology, Vol.3, No.2, 2005 water and Overflow water were measured ... content in pit sand The initial nitrogen content of Akadama sand in Tank A was higher than that of pit sand of Tank B The nitrogen content in both Tanks increased with time In both Tank A and B, the...

Ngày tải lên: 05/09/2013, 09:08

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... this study were of reagent grade Results and Discussion Extraction and partial purification of extracellular polymer The floc was satisfactorily deflocculated within 10 by sonication of the activated...

Ngày tải lên: 05/09/2013, 09:08

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution profile of P III and P IV Chromatofocusing chromatography...

Ngày tải lên: 18/02/2014, 16:20

... shown) Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and the first-strand cDNA for 3¢ rapid amplification of cDNA ends (RACE), prepared from total RNA of 6004 Nile ... including 56 bp of 5¢ UTR, 1482 bp of ORF, and 299 bp of 3¢ UTR The coding region of the sequence was translated into 494 amino acids, which included a typical signal peptide of 18 amino acids and two ... total volume of 50 lL containing 0.75 lL of a template, 150 pmol of a forward primer (A), 150 pmol of a reverse primer (B or C), · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5...

Ngày tải lên: 19/02/2014, 07:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

Ngày tải lên: 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...

Ngày tải lên: 21/02/2014, 00:20