... expression pattern of undifferentiated and differentiated humanembryonicstemcellsStemCells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cellsand hES cellsand between adult cellsand EB were significant (P < 0.05) Of note, ... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cellsand EB, and between hES cellsand adult cells was significant...
... endoderm differentiationof hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin ofhumanembryonicstemcellsand their ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonicstemcells The isolationof mouse andhumanembryonicstemcells (ES cells) heralded a new era in regenerative medicine, raising hopes ... and Fraser 2005) However, unhindered analysis of developmental events was made possible with the isolationofembryonicstemcells Extensive investigation of the properties and capabilities of...
... Transcription of LCR hypersensitive site in undifferentiated murine embryonicstemcellsand in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiationof murine embryonicstemcells We ... hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiationof murine embryonicstemcells PCR analysis of DNase I treated and reverse-transcribed total...
... chondrogenic differentiationof mesenchymal progenitor cellsand to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... Chondrogenic differentiationof cultured human mesenchymal stemcells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterizationof hypertrophy in chondrogenesis ofhuman ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant...
... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... expression and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiationof fetal mouse pancreatic b -cells ... Itskovitz-Eldor J: Differentiationofhumanembryonicstemcells into insulin-producing clusters StemCells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu YS, Hung SC: Fibronectin and pellet...
... pluripotent embryonicstemcellsanddifferentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure ofhumanembryonicstemcellsand spontaneous and ... embryonicstem cell; hESC, humanembryonicstem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... epigenomic profiling of a cohort ofhumanembryonicstem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming anddifferentiation potential of individual...
... osteogenic differentiationofhuman mesenchymal stemcells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiationofhuman ... stromal stemcells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stemcellsand the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal stem ... 2009;Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation J Cell Biochem (4):706-713 104 Kuhn NZ, Tuan RS 2010;Regulation of stemness andstem cell niche of mesenchymal...
... classes of pluripotent stemcells in humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cellsand recently the discovery of a fourth class of ... Adult stemcells Adult stemcells also known as somatic stemcells can be found in diverse tissues and organs The most common adult stemcells are the hematopoietic stem cells, mesechymal stemcells ... blood cellsand platelets, while skin stemcells give rise to the various types of skin cells Some recent reports suggest that adult stemcells such as haemopoietic stem cells, neuronal stem cells...
... Introduction 1.1 Humanembryonicstemcells 1.1.1 Overview and characteristics ofhumanembryonicstemcellsEmbryonicstem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Humanembryonicstemcells 1.1.1 Overview and characteristics ofhumanembryonicstemcells 1.1.2 Regulatory networks and transcription ... HES3 HumanEmbryonicStemCells Undifferentiated HES4 HumanEmbryonicStemCells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...
... VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from human blastocysts: ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and ... include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure ofhuman embryos and oocytes...
... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiationofhuman mesenchymal stemcells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction ofdifferentiationofhuman osteoblasts andhuman bone marrow stromal cells by bone morphogenetic protein-2 ... osteogenic differentiation studies Media of all cultures was changed every days Measurement of alkaline phosphatase activity and in vitro mineralisation Osteogenic differentiationofhuman MSCs...
... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and ... PLLA and TCPS In Figure b, OCN expression ofhuman MSCs on the PLLA nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells ... nanofibrous bundles and (12) reservoir 183 Figure 6.2 The macroscopic images of 2D (A) and 3D (B); and the SEM images of 2D (C) and 3D (D) at 20000X magnification of electrospun PLLA/Col nanofibrous...
... MODIFICATION OFHUMANEMBRYONICSTEMCELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... modification ofhumanembryonicstemcells Although lentiviral vectors are widely used in gene transfer to humanembryonicstem cells, there is still a need for vectors and methods to achieve efficient and ... BACULOVIRAL TRANSDUCTION OFHUMANEMBRYONICSTEMCELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source ofcells for the regenerative...
... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonicstemandembryonic germ cells derived from discarded human embryos and germ line stemcells ... lineage specific differentiationof hES cells is mandatory 1.5 HumanEmbryonicstemcells As the name suggests, the humanembryonicstemcells are derived from the early embryo These cells have the ... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterizationof hES cells 18 1.6 Differentiationof hES cells into...
... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Humanembryonicstemcells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... pellet system and the adherent micromass system, where the latter system is able to mediate a better loss of discordant cells through apoptosis and to induce a rapid onset of chondrogenic differentiation ... SYSTEM…………………………………………… 65 4.1.1 Pluripotency ofhumanembryonicstemcells …………………… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture conditions on hypertrophic...
... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiationof these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... ofstem cells, such as c-kit, BCRP-1, Notch-1, Sox-2 and BMI-1 (data not shown) To determine whether these cells also expressed the mRNAs of classical HIV receptor CD4 and co-receptor CXCR4 and ... HIV-1 and recombinant gp120 affect the survival anddifferentiationofhuman vessel wall-derived mesenchymal stemcells Retrovirology 2011 8:40 Submit your next manuscript to BioMed Central and...
... Cytokines and BMP-4 promote hematopoietic differentiationofhumanembryonicstemcells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Humanembryonicstem cell-derived NK cells ... H, Baker SC, et al.: Genome wide profiling ofhumanembryonicstemcells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC ... concentrations of BMP4 and VEGF, plus SCF, Tpo and FLT3 ligand (20 ng/ml; R&D Systems), and PTD-HoxB4 (1.5 μg/ml) to expand out BCs and their precursor After 3.5 days, EBs were collected and dissociated...
... 2.7 Embryonicstemcellsand induced pluripotent stemcellsEmbryonicstemcells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Humanembryonicstemcells (hESC) ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Humanembryonicstemcellsand induced pluripotent stemcells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable...
... characterizationof transplanted cells derived from human ES cells We induced differentiationofhuman ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... F G H I Figure Characterizationof the transplanted vascular cells derived from human ES cells (HES-3) Characterizationof the transplanted vascular cells derived from human ES cells (HES-3) A, ... Preparation ofhuman ECs and/ or MCs derived from human ES cells Maintenance ofhuman ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder layer to induce differentiation...
... Gokhale PJ, Andrews PW Culture andcharacterizationofhumanembryonicstemcellsStemCells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity ofhumanembryonic stem- derived cells ... CP, Rao MS Enrichment of neurons and neural precursors from humanembryonicstemcells Exp Neurol 2001, 172, 383-397 Chen U, Kosco M Differentiationof mouse embryonicstemcells in vitro; III ... limitation of adult stemcells is their inability to effectively grow in Humanembryonicstemcellsand therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells...