isolation characterization and differentiation of human embryonic stem cells

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant...

Ngày tải lên: 18/06/2014, 15:20

... endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... and Fraser 2005) However, unhindered analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of...

Ngày tải lên: 04/10/2015, 16:03

... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total...

Ngày tải lên: 07/03/2014, 12:20

... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant...

Ngày tải lên: 09/08/2014, 14:22

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... expression and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells ... Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu YS, Hung SC: Fibronectin and pellet...

Ngày tải lên: 10/08/2014, 05:21

... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...

Ngày tải lên: 11/08/2014, 12:21

... osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human ... stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal stem ... 2009;Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation J Cell Biochem (4):706-713 104 Kuhn NZ, Tuan RS 2010;Regulation of stemness and stem cell niche of mesenchymal...

Ngày tải lên: 11/09/2015, 09:16

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...

Ngày tải lên: 16/09/2015, 15:55

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...

Ngày tải lên: 16/10/2015, 11:58

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and ... include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes...

Ngày tải lên: 31/10/2012, 16:57

... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... osteogenic differentiation studies Media of all cultures was changed every days Measurement of alkaline phosphatase activity and in vitro mineralisation Osteogenic differentiation of human MSCs...

Ngày tải lên: 09/08/2014, 10:23

... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and ... PLLA and TCPS In Figure b, OCN expression of human MSCs on the PLLA nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells ... nanofibrous bundles and (12) reservoir 183 Figure 6.2 The macroscopic images of 2D (A) and 3D (B); and the SEM images of 2D (C) and 3D (D) at 20000X magnification of electrospun PLLA/Col nanofibrous...

Ngày tải lên: 09/09/2015, 10:07

... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... modification of human embryonic stem cells Although lentiviral vectors are widely used in gene transfer to human embryonic stem cells, there is still a need for vectors and methods to achieve efficient and ... BACULOVIRAL TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative...

Ngày tải lên: 11/09/2015, 14:38

... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells ... lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the ... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells into...

Ngày tải lên: 09/10/2015, 11:24

... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... pellet system and the adherent micromass system, where the latter system is able to mediate a better loss of discordant cells through apoptosis and to induce a rapid onset of chondrogenic differentiation ... SYSTEM…………………………………………… 65 4.1.1 Pluripotency of human embryonic stem cells …………………… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture conditions on hypertrophic...

Ngày tải lên: 11/09/2015, 09:57

... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... of stem cells, such as c-kit, BCRP-1, Notch-1, Sox-2 and BMI-1 (data not shown) To determine whether these cells also expressed the mRNAs of classical HIV receptor CD4 and co-receptor CXCR4 and ... HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells Retrovirology 2011 8:40 Submit your next manuscript to BioMed Central and...

Ngày tải lên: 13/08/2014, 01:20

... Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells ... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC ... concentrations of BMP4 and VEGF, plus SCF, Tpo and FLT3 ligand (20 ng/ml; R&D Systems), and PTD-HoxB4 (1.5 μg/ml) to expand out BCs and their precursor After 3.5 days, EBs were collected and dissociated...

Ngày tải lên: 14/08/2014, 08:20

... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable...

Ngày tải lên: 11/09/2015, 10:14

... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... F G H I Figure Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, ... Preparation of human ECs and/ or MCs derived from human ES cells Maintenance of human ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder layer to induce differentiation...

Ngày tải lên: 18/06/2014, 15:20

... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells ... CP, Rao MS Enrichment of neurons and neural precursors from human embryonic stem cells Exp Neurol 2001, 172, 383-397 Chen U, Kosco M Differentiation of mouse embryonic stem cells in vitro; III ... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells...

Ngày tải lên: 07/08/2014, 18:21