electrochemical detection of nucleic acids

... versatility of the electrode was tested Cyclic voltammetry was used for the detection of two nucleic acids samples mentioned above and the basic electrochemical behaviour of nucleic acids at the ... suitable tool for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals ... MWCNTs Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic...

Ngày tải lên: 21/06/2014, 03:20

... improved detection limit of 10 fM.33 1.1.3 Label-based detection of nucleic acids Nucleic acid does not have intrinsic properties sufficient for direct high-sensitivity detection Many types of nucleic ... Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization The nucleic acid is a polymer consists of monomers called nucleotides Each nucleotide composes of a nitrogenous ... improving the sensitivity and specificity of assays with an emphasis on the electrochemical nucleic acids detection 1.1.2 Label-free detection of nucleic acids There are attempts to measure the...

Ngày tải lên: 12/09/2015, 09:28

... through induced detection of additives attached to the DNA, and greatly extends the lower limit of From: Methods in Molecular Biology, Vol 162: Capillary Electrophoresis of Nucleic Acids, Vol 1: ... application to the detection of DNA mutation is a recent and rapidly developing area of research In the following subheadings, some of the mutation -detection methods that can make use of CE are discussed ... signal detection system (127) overcome some of the detection limitations of linear CAE and microchip devices and allow the parallel genotyping of 96 samples in about 120 s The integration of microreactive...

Ngày tải lên: 10/04/2014, 11:11

... Capillary electrophoresis of proteins and nucleic acids Annu Rev Biophys Biomol Struct 24, 579–610 Olivera, B M., Baine, P., and Davidson, N (1964) Electrophoresis of nucleic acids Biopolymers 2, ... multiplex detection of nucleic acid sequences: oligonucleotide assay and sequence-coded separation Nucleic Acids Res 22, 4527–4534 19 Day, D J Speiser, P W., White, P C., and Barany, F (1995) Detection ... the same, regardless of the type of the separation channel used (capillary or chip) The main advantage of the chip technology is the ease of fabrication of a large number of channels In comparison...

Ngày tải lên: 10/04/2014, 11:11

... adsorption of proteins or nucleic acids than in case of signal-off transduction Work is now in progress to extent this detection system to use as probes peptide nucleic acids (PNA) or locked nucleic acids ... concentration of 10−10 M; the limit of detection (LOD) is estimated around fM (see Section 2) The linear part of the calibration curve corresponds to an extremely high sensitivity of +7.5% per ... label-free and reagentless electrochemical DNA sensing based on SWNTs and an immobilized redox probe This system allows very specific detection of DNA but the limit of detection is only 0.1 mM 3D...

Ngày tải lên: 02/07/2014, 14:14

... Table of Contents Table of Contents Acknowledgements i Table of Contents iii Summary vii List of Tables ix List of Figures xi List of Abbreviations ... the detection of Dengue infection .4 1.3 Advanced methods fabricated for the detection of Dengue infection 12 1.4 Scope of research 19 1.5 References 21 Chapter 2: Electrochemical ... amplifier as they are capable of encapsulating a large quantities of a wide range of substances such as fluorophores and enzymes The mode of detection depends on the type of marker molecules encapsulated...

Ngày tải lên: 10/09/2015, 09:11

... diffusion of ions at the electrode/electrolyte interface DPV has demonstrated advantages of high sensitivity and the lowest detection limit by amplification of the electrochemical signal of polypyrrole ... oxidation of the polypyrrole backbone The presence of only one potential peak for the copolymer demonstrates a good distribution of both monomers in the film In the case of the formation of a block of ... surface of the prism in the electrochemical cell containing 50 ␮L of a 10 mM solution of pyrrole (py) and 3-N-hydroxyphthalimido pyrrole (pyNHP) monomers and 0.1 M LiClO4 in acetonitrile The ratio of...

Ngày tải lên: 13/09/2015, 18:05

... Time (minutes) Figure of g20 nucleic acid during infection cus sp WH7803 by cyanophage strain S-PM2 of SynechococDetection Detection of g20 nucleic acid during infection of Synechococcus sp WH7803 ... polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required (b) Detection of RNA signal ... Sequences of probes for the amplification of signal RNA, capture and detection of SMART signal, and of synthetic product for ELOSA standard curve have all been published previously [1,4] Page of (page...

Ngày tải lên: 18/06/2014, 18:20

... of an additional oligonucleotide modified by the introduction of locked nucleic acids (LNA) (ii) The less conserved glycoprotein B (gB) gene was amplified with degenerate primers of limited detection ... in the detection of PtroLCV-1 [7] To unravel the simultaneous presence of other herpesviruses, the panherpes DPOL PCR was carried out in the presence of LNAPtroLCV1, using spleen samples of Noah ... (UL54; partial) Figure Amplification of an kbp locus of CgueCMV-1.1 and CgueCMV-1.2 Amplification of an kbp locus of CgueCMV-1.1 and CgueCMV-1.2 At the top of the figure, the betaherpesvirus ORFs...

Ngày tải lên: 18/06/2014, 18:20

... Time (minutes) Figure of g20 nucleic acid during infection cus sp WH7803 by cyanophage strain S-PM2 of SynechococDetection Detection of g20 nucleic acid during infection of Synechococcus sp WH7803 ... polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required (b) Detection of RNA signal ... Sequences of probes for the amplification of signal RNA, capture and detection of SMART signal, and of synthetic product for ELOSA standard curve have all been published previously [1,4] Page of (page...

Ngày tải lên: 20/06/2014, 01:20

... reported the design of protein vectors that are capable of self-assembly with nucleic acids The key principle behind our design of the protein vectors is the use of nucleic acids which encode ... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... incorporated into nucleic acids and primers Page of 16 (page number not for citation purposes) Journal of Nanobiotechnology 2003, http://www.jnanobiotechnology.com/content/1/1/1 Figure Detection of the...

Ngày tải lên: 11/08/2014, 00:21

... and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and 10 copies of ssDNA plasmids No fluorescence was detected ... conducted in a total volume of 25 μl containing 12.5 μl of 2× RT-PCR Reaction Mix, 400 nM of each primer, 120 nM of probe, 0.5 μl of iScript Reverse Transcriptase, and μl of template Optimized rRT-PCR ... copies of ssDNA plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from...

Ngày tải lên: 12/08/2014, 04:21

... contamination of PCR reagents A third report by Sato et al [10] describes the detection of MLV-encoding nucleic acids in PCR reagents obtained from a commercial supplier (Invitrogen) Analyses of individual ... identify three potential sources of contamination in PCR-based studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human ... claims of MLV-related viruses in prostate cancer and CFS patients Future assessments of the prevalence of XMRV should include more rigorous PCR and phylogenetic tests to exclude the possibility of...

Ngày tải lên: 13/08/2014, 01:20

... induction of non-transcriptional responses to nucleic acids A common feature of the recognition of non-self nucleic acids by TLRs, RLRs and DAI is their ability to induce robust activation of genes ... recognition of microbial nucleic acids, they can in some circumstances lead to the recognition of host DNA and RNA and the development of autoimmunity [47] TLRs specific for nucleic acids are normally ... selectively enhances DNA-mediated induction of type I interferons and of other genes involved in innate immunity Thus, DAI may be central to detection of the DNA of viruses, bacteria, fungi and parasites...

Ngày tải lên: 14/08/2014, 08:20

... thermal detection biosensing mechanism Thermal detection biosensors show limit of detection in mM/l range while analysis of clinically relevant compound is necessary even up to lower level of detection ... technique of DNA detection require minimal sample preparation step for analyte detection and show very low detection limit Among non pcr based DNA detection, the focus of the study is only based on electrochemical ... sensitivity and low limit of detection An electrochemical nanoporous alumina membrane-based label free DNA biosensor is developed for detection of target DNA strands of Legionella sp using 5'-aminated...

Ngày tải lên: 08/09/2015, 18:11

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

Ngày tải lên: 03/11/2012, 10:58

... chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences S9 fraction of phenobarbital/5,6-benzoflabone-pretreated male Sprague-Dawley (SD) rat liver ... We compared the mutagenic activity (shown as the number of revertants per nanomolecule of chemicals, rev./nmol) of YG strains with that of the conventional TA strains as shown in Fig YG1041 showed ... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts...

Ngày tải lên: 05/09/2013, 08:40

... distribution of the sediment, especially composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted ... capsid of approximately 25 nm diameter This virus was also used by Gerba et al (1977) for evaluation of the method Recovery rate was evaluated by dividing amount of spiked Poliovirus by amount of ... procedure of Craig et al (2002) with some modifications: mL double-distilled water was used instead of mL 0.1% peptone water; a vortex mixer was used for 15 sec instead of hand - 310 - Journal of Water...

Ngày tải lên: 05/09/2013, 10:15

... periods may result to the development of oseltamivir-resistant influenza viruses Furthermore, Japan is at the top of the list of countries for per capita consumption of Oseltamivir (Soderstrom et al., ... and 200.06, respectively Quantifications of OP, OC and OC-D3 were based on calibration curves from to 1000 ng/L The limit of detection (LOD) and limit of quantification (LOQ) values for OP, OC ... ST-2 from the 34th week of 2009 to the 7th week of 2010 period were obtained from Osaka Prefectural Institute of Public Health (2010) An index value gives the total number of influenza cases (average)...

Ngày tải lên: 05/09/2013, 10:15