em tomography and multiplex detection of nucleic acids and protein in situ
Báo cáo hóa học: " Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids" pot
... voltammograms of (D) single strand oligonucleotide influenza (13 μg/mL), and (E) double strand genomic DNA (15 μg/mL) In insets: signals of all nucleic acid bases after baseline correction and smoothing of ... for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals Methods Chemicals ... Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize Anal Chem 2003, 75:2663-2669...
- 5
- 382
- 0
Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids
... pentose sugar and a phosphate group In deoxyribonucleic acid (DNA), the bases are the purine adenine (A) and guanine (G) as well as the pyrimidines cytosine (C) and thymine (T) In deoxyribonucleic ... translocation of BCR-ABL The structures of BCR and ABL are shown in (A) and (B), respectively Boxes denote exons and lines refer to introns (C) Depending on the location of breakpoints (arrows) in BCR, ... with an emphasis on the electrochemical nucleic acids detection 1.1.2 Label-free detection of nucleic acids There are attempts to measure the hybridization signal directly in the absence of a secondary...
- 226
- 346
- 0
capillary electrophoresis of nucleic acids, volume i
... to identify DNA-binding proteins of interest by the retardation of electrophoretic mobility of the complex The dissociation constant for DNA binding of specific proteins or protein regions can ... immobilized protein, creating specific retardation of a mobile DNA ligand, or immobilized DNA specifically retarding a mobile protein ligand Both CAGE and CEMSA benefit in speed and utility when miniaturized ... use of intercalating dyes These dyes bind to the DNA molecule by inserting themselves into or along the DNA helix, affecting the configuration of the aromatic rings of the dye and enhancing the...
- 456
- 377
- 0
capillary electrophoresis of nucleic acids, volume ii
... boundary of the faster migrating zone is leaving, and the front boundary of slower migrating zone is entering the detection window at the same time If the lengths of a detection window and an injection ... advancement in protein and DNA research that command the development of adequate analytical instrumentation capable of high-throughput analysis of minute amounts of complex samples Attempts to integrate ... nm/emission 520 nm and 560 nm) Polymorphism in the short tandem repeats, consisting from three adjacent repeat regions of CT, CA, and GC and situated between 979 and 1039 position of Endothelin...
- 365
- 528
- 0
Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... [3,4] We and others have demonstrated the existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely ... against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of ... detected RV2 rhadinovirus DNA in of 30 macaques; of 20 M nemestrina, of M fascicularis and of macaques whose species is not known In these macaques, the viral copy number was determined to range...
- 12
- 509
- 0
báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... [3,4] We and others have demonstrated the existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely ... against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of ... detected RV2 rhadinovirus DNA in of 30 macaques; of 20 M nemestrina, of M fascicularis and of macaques whose species is not known In these macaques, the viral copy number was determined to range...
- 12
- 471
- 0
Báo cáo khoa học: "Study protocol of the German "Registry for the Detection of Late Sequelae after Radiotherapy in Childhood and " ppsx
... late effects after cancer therapy in childhood including chemotherapy, surgery and radiation has been of rising interest [8,9] Several study groups in a variety of countries have developed strategies ... not sufficient in view of answering open questions regarding the risks of radiotherapy in childhood and adolescence Methods/Design To overcome the described lack of information and to receive ... coordinator of RiSK, participated in the development of the study trial and drafted the manuscript AS, HP, CR, BP, BT, RDK and KD participated in the design of the registry study and are involved...
- 4
- 397
- 0
Báo cáo y học: "Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers" pot
... [9] for a final volume of 20 mL Each sample was inoculated with 1% of 100X antibiotic/antimycotic and 0.1% of 50 ug per mL of gentamicin sulfate and incubated at 37°C for hours Post incubation ... Suitability of infection of cells in suspension for detection of herpes simplex virus J Clin Microbiol 1991, 1554-1557 Reynolds KA, Gerba CP, Pepper IL: Detection of infectious enteroviruses by an integrated ... viruses) infection In a clinical setting Luker et al demonstrated that even in low numbers virus infection in a suspension-infection method is detected sooner than the monolayer method and that...
- 4
- 241
- 0
báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... Analysis of Bet v isoforms by Q-TOF LC-MSE The tryptic digests of the 1618 kDa bands were examined in detail to elucidate the expression of separate Bet v isoforms in pollen Trypsin cleaves proteins ... (01 and 02) were expressed in birch pollen We showed that Q-TOF LC-MSE allowed fast screening of Bet v isoforms in birch pollen by determining presence and relative abundances of individual isoforms ... the predicted proteins Protein sequences of birch PR-10 isoforms in GenBank (overview in: Schenk et al., 2006), keratin, trypsin and Bet v (AJ311666) were added to the database Protein extraction...
- 15
- 349
- 0
Analysis and semi automated detection of design level similarity patterns in software
... Chapter Introduction With the ever increasing role of computing in all aspects of life, more and more software is being created A large part of the software costs go to the maintenance of a system ... calls and inlining the functions Cloning can also help in better program understanding, by keeping the software architectures simple and avoiding complicated abstractions [KG06] Clone detection and ... system Refactoring [Opd92][Fow99] is the state -of- the-art in removing clones from the code Refactoring is the technique of improving the internal design of the software system without changing...
- 199
- 481
- 0
Báo cáo khoa học: Emerging tools for real-time label-free detection of interactions on functional protein microarrays ppt
... to study binding interactions with proteins [56,83], including the interactions between proteins and small organic molecules, peptides, nucleic acids, and protein drugs The detection principle ... building blocks of the protein [4,5,54] Espejo et al characterized the function of protein domains by purifying and displaying over 200 protein fragments, of which 145 were known protein domains (PDZ, ... spotting of expression plasmids, instead of purified proteins, on the array surface and using a mammalian cell-free expression system to express the proteins in situ at the time of the assay All proteins...
- 14
- 551
- 0
Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... recognized as a member of the immunoglobulin protein superfamily [12] Many studies have reported detection of gastric cells in blood [13], bone marrow [14], and peritoneal washing [15] of gastric cancer ... cytokeratins 8, 18, and 19 in bone marrow and limited expression of cytokeratins and 20 by carcinoma cells: inherent limitations for RT-PCR in the detection of isolated tumor cells Lab Invest 2001, 81:1351-1361 ... Akiyoshi T: Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcriptionpolymerase chain reaction in patients with gastrointestinal or...
- 8
- 439
- 0
báo cáo hóa học: " The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice" pptx
... exposure to nicotine and chlorpyrifos, alone and in combination, leads to persistently elevated expression of glial fibrillary acidic protein in the cerebellum of the offspring in late puberty ... resulted in an increase in the occurrences of reported CNS symptoms by more than threefold, indicating a possible link between stress and increased BBB permeability [10] One of the proteins associated ... the average value of maximum binding wells (B0) To obtain standard curve, concentration standards were plotted against logit values Logit of the data was then employed in Microsoft Excel to get...
- 9
- 451
- 0
Báo cáo khoa học: "Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island" doc
... )'3-GGTCGTCGCTGTCTACTGCACTCG-'5( 2F remirp drawrof eht htiw demrofrep ,RCP dnuor-dnoceS )'3-CGTT AGTTCCGACCGCGAGACATC-'5( 1R remirp esrever eht dna )'3-CTCAGTTGTAGTCCATGTCCTCGA-'5( 1F remirp drawrof eht htiw )pb( ... detcefni sgip ,stnemirepxe ruo nI noitatnalpsnartonex fo stneipicer desimorpmoconummi ni cinegohtap emoceb yam ti ,snoitcefni latnemirepxe ro larutan ni snamuh dna sgip rof lacinilcbus eb ot sraeppa ... erew selpmas ,noitaxif retfA ygolohtapotsih rof nilamrof dereffub lartuen %01 ni dexif saw trap gniniamer eht elihw ,)RCP( noitcaer niahc esaremylop rof Co07− ta nezorf saw revil hcae fo driht-enO...
- 5
- 349
- 0
Báo cáo khoa học: "PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases" doc
... detatipicerp saw AND )1 : 42( lohocla lymaosi :mroforolhc dna )1 : 1( mroforolhc :lonehp htiw noitcartxe yb deifirup saw AND Co56 ta nim 03 rof detabucni dna )lCaN M 7.0 ni edimorb muinomma lyhtemirt lycedaxeh ... secneuqes remirp dna sretemarap noitacifilpma eht ,yduts tneserp eht nI )ASU ,hcirdlA amgiS( esaremylop AND qaT U1 dna ,)aidnI ,ieneG erolagnaB( sremirp eht fo hcae fo lomp 01 ,sPTNd ruof eht fo ... airetcab morf AND cimoneg fo noitaraperP K nosliW 91 6511-0511 ,26 ,9991 torP dooF J stcudorp yriad dna serutluc retrats taem ni fo noitceted rof metsys desab-noitcaer niahc esaremylop detegrat...
- 4
- 138
- 0
Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
... with 200 μl of wash buffer (PBS containing 0.05% [v/v] Tween-20 and 0.01% bovine serum albumin), and once with 200 μl of PBS To each well, 100 μl of biotinylated capture probe (0.1 μM in 50 mM potassium ... cut into 4.5 mm × 7.5 cm strips Streptavidin was diluted in 0.4 M NaHCO3/Na2CO3 buffer (pH 9.0) containing 5% methanol in a final concentration of 20 pmol/μl Streptavidin was spotted on the membrane ... of detection, liposomes were lysed with a detergent, releasing the otherwise selfquenched SRB dye and detected using fluorescence (Fig 3) A limit of detection of 0.1 nM was obtained calculating...
- 8
- 385
- 0
Báo cáo khoa học: "Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer" ppsx
... setting and record the concordance between IHC and FISH in 50 cases of invasive ductal carcinoma of breast Methods Study Design The study population consists of 50 cases of invasive ductal carcinoma ... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... Discussion and protein using IHC are the two most accessible and feasible methods used in clinical diagnosis, and certain kits or antibodies are approved by the FDA (U.S Food and Drug Administration)...
- 6
- 360
- 0
Báo cáo y học: "Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis" potx
... preparation In an independent AIA study, samples of knee-joint SM, inguinal LNs, and spleen were obtained from rats killed at six time points: day 0, hours, day 1, day 3, and day of AIA Cytokine protein ... course of AIA (Table 1, in conjunction with Fig and Table 3, underlining the predominantly local character of AIA Cytokine mRNA and protein expression in the synovial membrane IL-1β IL-1β mRNA increased ... ≤ 0.05 lower joint swelling in the arthritis series used for determination of protein (data not shown) allowed only a qualitative comparison of mRNA and protein levels in the SM and the other...
- 13
- 455
- 0
Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx
... 2) Staining was particularly prominent in the cytoplasm of the chondrocyte-like cells of the NP and IAF, with both single cells and those in clusters showing immunopositivity (Figures and 2) ... endothelial cells of Figure Examples of immunohistochemical staining for CDMPs in human intervertebral disc (row A) Cartilage derived morphogenetic protein (CDMP 1) and for CDMPs in human intervertebral ... vessels and nerves resulting in generation of Here we demonstrate the synthesis and localisation of CDMP and CDMP within human IVDs Although a small decrease in the proportion of cells within the...
- 10
- 437
- 0
Báo cáo sinh học: "Optimal design for the detection of a major gene segregation in crosses" potx
... where both F2 and backcrosses were present The proportiont of individuals belonging to the ’segregating groups’ increased between C10 and C19, C20 and C26, and C3 and C5 The proportion of F2s to ... linear for power under 70% and, in this linear part, the slope (ie the gain in power = = = per extra individual measured) increases with a The resulting increase in size of the design required for ... proportion of segregating (n 90) and non-segregating populations (n = n l n 30), ie with at ratio of 1/2 In contrast, in the C2 situations, this optimal proportion seems to differ according to whether...
- 11
- 368
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25