... Edmonton, AB, CanadaType IIa bacteriocins, which are isolated from lactic acidbacteria that are useful for food preservation, are potentantimicrobial peptides with considerable potential astherapeutic ... structural basis of the inhibition ofthe antimicrobial activity of CbnB2 by the leader and to assist future analysis ofthe interaction of preCbnB2 with its ABC transporter protease, it isessential ... Theseinvestigations were supported by the Natural Sciences and EngineeringResearch Council of Canada, the Alberta Heritage Foundation forMedical Research, CanBiocin Ltd (Edmonton, AB), and the CanadaResearch...
... Each of these variants retains a catalytic activity against yeast RNA comparable with that of parent mBS, indicating that a native conformation ispresent. A further indication ofthe similarity ... 5Â-GAGTGCGGCCGCAAGCTTGGGCTG-3Â, had an estimated Tm of 82 C. The reverse anking primer sequence, 5Â-ATATACATATGAAAGAAAG-3Â, had a calculated Tm of 42 C. The mutagenic primers for each variant are: P1 9A ... forms.However, after 48 h, a slight prevalence ofthe MxM form can be seen in the BS-RNase and P1 9A variant, whereas the L28Q and P1 9A/ L28Q variants still contain comparableamounts of MxM and...
... catalytic pockets in the asymmetric unit, and the glutamate-binding modes are identical to eachother (Fig. 2A) . The a- carboxyl and a- amino groups of the bound glutamate are at the bottom of the pocket, ... the Quikchange Site-Directed Mutagenesis kit (Stratagene, LaJolla, CA, USA) with forward primer 5Â-GAAACGATGCATTTGTCCTATGCCGACCGTGCGTC-3Â and reverseprimer 5Â-GACGCACGGTCGGCATAGGACAAATGCATCGTTTC-3Â. ... 5Â-CATATGGATGAGTACAAACAAGTAGATG-3Â and reverse primer 5Â-GGATCCTCGAGCTCATTTACGTTTTAAATTAATGCCGAT-3Â (underlin-ed sequences indicate NdeI and BamHI sites, respectively). The PCR product was initially...
... the provinceinexchangefor the support of the Polesin the Austrianparliament. with a Polisharmy organizedinAmerica, of un-Amer-icanized Polishimmigrants,begananoffensiveagainst the Ukrainians,attackingthem from the rear. ... a temporaryoverthrowby the Germanmilitaryforcewasreestablished.In the latterpart of 1918, the Ukrainians of EasternGa-licia(also predominantlyUkrainianandanciently, Accordingto the InternationalEncyclopedia, the entire Austrianprovince of Galicia(westernandeast-ern) ... Ukrainians.Accordingto the EncyclopediaBrittanica, the formerpredominatein the West andin the bigtowns,and the latterin the East.Accordingtoofficial statistics of the Austrianpro-vincialgovernment of Galicia, preparedandpublishedbyleaders of Polishpolitical...
... to achieve the aims ofthe thesis and answers to the above research questions, the participants ofthe study include: a group of 3 teachers of English at Ke Sat High School. All of them are ... the reading tasks. It has advantages as well as disadvantages for the teachers and the students. The teachers do not need to design tasks. However, available tasks cause difficulty. The teachers ... write about the things relating to the theme in the next parts. 2.2. Characteristics of reading passages and reading tasks in the textbook 2.2.1. Characteristics of reading passages A reading...
... Data PacketsThere are two types of data packets each capable of transmitting 0 to 1023 bytes of data. Data0 Data1Data packets have the following formatSync PID Data CRC16 EOP Handshake ... correctly, the function caneither reply witha DATA packet containing the bulk data to be sent, or a stall packet indicating the endpoint has had a error or a NAK packet indicating to the host that the ... it has transferred the exact amount of data requested,transferred a packet less than the maximum endpoint size of transferred a zero-length packet. The above diagram shows the format ofa bulk...
... substantiated in this chapter as the participants, the instruments as well as the data analysis are discussed in detail. 3.1. Participants The process of data collection involved the participation ... because of what they are being taught in the classroom. 62.5% ofthe teachers admit that their students lack of appropriate reading strategies so they often fail to finish their reading tasks ... extensive reading are of great importance as they act as a means to gain knowledge. 2.3. Reading in second language learning and teaching 2.3.1. The importance of teaching reading in the L2 classroom...
... ringare Asn53, Gln70, Trp78, Ser114, Arg180 and Asp181(Fig. 3C). The interactions ofthe blades in the axisarea are of polar character. The water channel in the apo formofthe enzyme runs along the ... probably a consequence of a mistake incorporated by the TaKaRa Ex TaqÔ poly-merase and did not affect the enzymatic activity.MS The molecular mass ofthe recombinant b-fructofuranosi-dase was ... Technical University of Lodz, PolandIntroductionBifidobacteria are found in human and animal gastro-intestinal tracts, as well as in the oral cavity and the vagina [1]. They are among the first bacteria...
... by PCR with oligonucleotides 5Â-CCCCATATGATGAGGACAGATTCATCAAAAATG-3Â and 5Â-CATGGATCCTCATTTTTCTTCATTTTGAGGC-3Â. The amplied PCR fragmentwas inserted into plasmid vector pET between the endo-nuclease ... of Lowry et al. [14] and the purity was examinedby SDS/PAGE [15].Assay of human PKIb activity The activity of purified human PKIb was assayed by the inhibition ofthe catalytic subunit of cAPK ... signal ATTAAA (1018–1023) and poly (A) tail (Fig. 1). The human PKIb proteinpredicted by the open reading frame is 78 amino acids inlength witha calculated molecular mass of 8468.2 Da andPI of...
... Surolia N, RamachandraRao SR & Surolia A (2002)Paradigm shifts in malaria parasite biochemistryand anti-malarial chemotherapy. Bioessays 24, 192–196.5 Ramya TNC, Surolia N & Surolia A ... characterization of Plasmodium acyl carrier protein can serve as a prototype for the analysis of the conformational stability of other acyl carrier proteins.AbbreviationsAAS, acyl-ACP synthase; ... and differentiation, and the occurrence of a different type (type I) of fatty acid biosyntheticpathway in the human host from that ofthe malariaparasite, make this pathway an attractive target...
... enzyme[16,49]. The main forms of hTH1 are also indicated by arrows in PanelD. The [125I]Ub-labelled conjugates of hTH1 are v isualized as diagonalspots of radioactivity in the autoradiogram (Panel C and ... counted in a b scanner ( Packard Instant Imager,Packard Inc., Canberra, Australia) or exposed to X-ray filmfor autoradiography.For Western blot analysis of chromaffin granule mem-brane proteins ... (C). The multiple molecular forms of hTH1 have a molecular mass for the subunit of 62 kDa; the doublet witha more acidic p I a nd a molecular mass of 100 kDa r epresents presumably the E1...