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Tài liệu Supply the correct form of the verbs1 docx

Tài liệu Supply the correct form of the verbs1 docx

... live in a snake. 14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct form of the verbs 1. Cats could fly if they (have) wings. 2. If Peter ... (not,want) to live in a snake. 14. My brother managed to kill the snake just at the time when I (be) almost exhausted. If he (be) a little late, I (kill) by the snake. 15. Had I know you were ill, ... snake. 14. My brother managed to kill the snake just at the time when I (be) had been almost exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake. 15. Had...

Ngày tải lên: 23/01/2014, 07:20

5 1K 3
Báo cáo khoa học: Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4 doc

Báo cáo khoa học: Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4 doc

... 4996–5007 ª 2005 FEBS the backbone and side chain of Asn34 and the side chain of Lys24. This crystal contact stabilizes the structure of the leading side of the foot loop. The putative internal ... C-terminus, while Arg206 is on the top of the molecule, in the loop leaving helix 6 and it points in the opposite direction to the other basic residues (Fig. 2B). The structure of the NLS is almost identical ... to the side chain of Asn81. The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts. Comparison with the structure of the soluble form...

Ngày tải lên: 23/03/2014, 15:21

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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

... biological function of the pro -form of bone morphogenetic protein-2 (BMP-2), a member of the transforming growth factor b (TGFb) ⁄ BLP superfamily. The role of the 263 amino acids of the pro-peptide ... Furthermore, the pro-peptide of GDF-8 impairs interaction of the mature part with its receptors [16]. In the case of BMP-9, the pro-pep- tide appears not to alter significantly the activity of the mature ... interested in the role of the 263 amino acid pro-peptide of BMP-2, because evidence accumulated over recent years has shown that the pro-forms of growth factors can modulate the activities of the mature...

Ngày tải lên: 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... rates) prior to and after the transition of the enzyme to a more active form, respectively, t is the time and s is the lag- time. The rate constant, k, for the activation of the enzyme is obtained ... rate is the initial turnover of the enzyme with a maximum of k cat , S 0.5 is the concentration of substrate S at half-maximal saturation of the enzyme and n is the Hill coefficient. Equation ... lip prevented binding of the C-terminal residues over the active site, or the absence of the C-terminal residues caused the movement of the lip (Fig. 1B). In addition, the C a chain between amino acid...

Ngày tải lên: 18/02/2014, 16:20

11 577 0
Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... the SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown. In the process of cloning of human Noxo1, some spliced transcripts of the ... To compare the amounts of the two variants expressed in the testis, we performed the PCR using the primers ‘d’ and ‘c’, where the cDNAs for both Noxo1b and Noxo1c are amplified as the insert region ... between the pair of primers (Fig. 2A). The lengths of the PCR products of Noxo1b and Noxo1c were 225 and 240 bp, respectively. To delineate the difference in the two products, we subjec- ted the...

Ngày tải lên: 19/02/2014, 06:20

15 633 0
Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

... enzyme. The catalytic mechanism of hydrolysis The fructose present in the crystal structure of the complex is the result of hydrolysis of raffinose. The conformation of the sugar observed in the electron density ... attack on the anomeric carbon of fructose. The leaving group is carboxylate of Asp54. Dimerization The asymmetric units of the crystals of both the apo and complexed form of the enzyme consist of dimers with ... blades in the axis area are of polar character. The water channel in the apo form of the enzyme runs along the axis of the b-propeller through the whole domain and is only blocked by Cys236 in the...

Ngày tải lên: 06/03/2014, 00:21

17 522 0
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO that exhibits reduced electrophoretic mobility compared with the IIA isoform. Whereas the ... replicases in the eukary- otic cell [22]. Therefore, we tested the effect of the replicative state of the cell on the interaction between Pol e and RNA pol II. A possible confinement of the interaction ... specifically in the G 1 phase of the cell cycle. This effect could be attributed to inhi- bition of transcription of cell cycle-regulated genes at this phase of the cell cycle [51]. Nevertheless, it...

Ngày tải lên: 07/03/2014, 11:20

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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase. J. Biol. Chem. 272, 20011–20020. 20. Byler, D.M. & Susi, H. (1986) Examination of the secondary structure ... Characterization of PKI gamma, a novel isoform of the protein kinase inhibitor of cAMP- dependent protein kinase. J. Biol. Chem. 272, 18169–18178. 4. Gamm, D.M. & Uhler, M.D. (1995) Isoform-specific ... showedasinglebandbySDS/PAGE (Fig. 2). The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 10 4 unitÆmg )1 (Fig. 3A) .The K i value determined...

Ngày tải lên: 07/03/2014, 15:20

6 531 0
Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot

Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot

... effect on the thermal unfolding of proCPY. The P m value for CPY was higher than that of its unglycosylated form, though the DV and DG P values were almost the same. The P m value of proCPY was ... the corresponding values of proCPY and Dgly proCPY (Fig. 2B), indicating that the precursor form was less thermally stable than the mature form, regardless of the carbohydrate moiety. After the ... unfolding of the unglycosylated form. At high pressure, in the glycosylated forms the carbohy- drate moiety of CPY and proCPY would be hydrated to compensate the volume contraction and the protein...

Ngày tải lên: 07/03/2014, 21:20

7 439 0
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... iden- tified as the major and the minor forms of the holo- ACP structure, on the basis of their percentage contri- butions (65% and 35%, respectively) to the overall structure of the protein. The structures ... both forms have the same amino acid sequence, and they have similar secondary structures, it was found that the – DG of unfolding of the holo form was lower than that of the apo form at all the ... unfolding thermodynamic parameters of the two forms are given in Tables 1–3. In the entire experimental regime, it is seen that the holo form presents better stability than the other form (Fig. 4C). The...

Ngày tải lên: 16/03/2014, 10:20

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Báo cáo khoa học: "TWO THEORIES FOR COMPUTING THE LOGICAL FORM OF MASS EXPRESSIONS" doc

Báo cáo khoa học: "TWO THEORIES FOR COMPUTING THE LOGICAL FORM OF MASS EXPRESSIONS" doc

... true of unions of elements of the semilattice). These predicates are not only true of the conventional kinds but also of conventional servings such as the bottle of wine on the table or the ... speaking, the theories view the kinds of M as forming an upper semilattice of kinds with M at the top. This is a "formal" semilattiee in that the union of any two elements of it is ... for the two theories of mass terms. The p-rules are for the semantic occurrence approach while the s-rules are for the syntactic expression approach. The ontological underpinnings of these theories...

Ngày tải lên: 24/03/2014, 01:21

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