the standard form of the equation

Ngày tải lên: 26/12/2013, 01:18

... live in a snake. 14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct form of the verbs 1. Cats could fly if they (have) wings. 2. If Peter ... (not,want) to live in a snake. 14. My brother managed to kill the snake just at the time when I (be) almost exhausted. If he (be) a little late, I (kill) by the snake. 15. Had I know you were ill, ... snake. 14. My brother managed to kill the snake just at the time when I (be) had been almost exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake. 15. Had...

Ngày tải lên: 23/01/2014, 07:20

... 4996–5007 ª 2005 FEBS the backbone and side chain of Asn34 and the side chain of Lys24. This crystal contact stabilizes the structure of the leading side of the foot loop. The putative internal ... C-terminus, while Arg206 is on the top of the molecule, in the loop leaving helix 6 and it points in the opposite direction to the other basic residues (Fig. 2B). The structure of the NLS is almost identical ... to the side chain of Asn81. The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts. Comparison with the structure of the soluble form...

Ngày tải lên: 23/03/2014, 15:21

... [43]. The present study further illustrates the importance of the eight- peptide sequence 57–64 in maintaining the inhibitory effect of the original 20-peptide a IIb 49–68. The N-terminal region of ... were performed in the presence of PAC-1, a ligand-mimetic anti-a IIb b 3 that contains the RYD sequence (an RGD mimic) in the CDR3 region of the heavy chain. PAC-1 binds to the activated form of a IIb b 3 and ... with this lig- and. The latter is further supported by the finding that the above peptides did not interfere with the binding of PAC-1 to the activated form of a IIb b 3 . Furthermore, a IIb 313–332 was...

Ngày tải lên: 31/03/2014, 07:20

... proportion of the 125 I released (Fig. 7A) may be derived from the diffusion of low M r 125 I from the cells. In contrast, the slower release of 125 I-Tf (Fig. 7B) was consistent with the efflux of the Fig. ... examining the uptake of the 125 I-sMTf or 125 I-Tf, the proteins were also saturated with 59 Fe by the procedures described above. These dual labelling experiments enabled examination of the uptake of ... increased effi- cacy of conjugates compared to free agents [49,50]. It is possible that the use of the conjugate may enable the delivery of the agent to the lysosomal compartment that then results in...

Ngày tải lên: 31/03/2014, 09:20

... biological function of the pro -form of bone morphogenetic protein-2 (BMP-2), a member of the transforming growth factor b (TGFb) ⁄ BLP superfamily. The role of the 263 amino acids of the pro-peptide ... Furthermore, the pro-peptide of GDF-8 impairs interaction of the mature part with its receptors [16]. In the case of BMP-9, the pro-pep- tide appears not to alter significantly the activity of the mature ... interested in the role of the 263 amino acid pro-peptide of BMP-2, because evidence accumulated over recent years has shown that the pro-forms of growth factors can modulate the activities of the mature...

Ngày tải lên: 18/02/2014, 06:20

... rates) prior to and after the transition of the enzyme to a more active form, respectively, t is the time and s is the lag- time. The rate constant, k, for the activation of the enzyme is obtained ... rate is the initial turnover of the enzyme with a maximum of k cat , S 0.5 is the concentration of substrate S at half-maximal saturation of the enzyme and n is the Hill coefficient. Equation ... lip prevented binding of the C-terminal residues over the active site, or the absence of the C-terminal residues caused the movement of the lip (Fig. 1B). In addition, the C a chain between amino acid...

Ngày tải lên: 18/02/2014, 16:20

... the SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown. In the process of cloning of human Noxo1, some spliced transcripts of the ... To compare the amounts of the two variants expressed in the testis, we performed the PCR using the primers ‘d’ and ‘c’, where the cDNAs for both Noxo1b and Noxo1c are amplified as the insert region ... between the pair of primers (Fig. 2A). The lengths of the PCR products of Noxo1b and Noxo1c were 225 and 240 bp, respectively. To delineate the difference in the two products, we subjec- ted the...

Ngày tải lên: 19/02/2014, 06:20

... enzyme. The catalytic mechanism of hydrolysis The fructose present in the crystal structure of the complex is the result of hydrolysis of raffinose. The conformation of the sugar observed in the electron density ... attack on the anomeric carbon of fructose. The leaving group is carboxylate of Asp54. Dimerization The asymmetric units of the crystals of both the apo and complexed form of the enzyme consist of dimers with ... blades in the axis area are of polar character. The water channel in the apo form of the enzyme runs along the axis of the b-propeller through the whole domain and is only blocked by Cys236 in the...

Ngày tải lên: 06/03/2014, 00:21

... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO that exhibits reduced electrophoretic mobility compared with the IIA isoform. Whereas the ... replicases in the eukary- otic cell [22]. Therefore, we tested the effect of the replicative state of the cell on the interaction between Pol e and RNA pol II. A possible confinement of the interaction ... specifically in the G 1 phase of the cell cycle. This effect could be attributed to inhi- bition of transcription of cell cycle-regulated genes at this phase of the cell cycle [51]. Nevertheless, it...

Ngày tải lên: 07/03/2014, 11:20

... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase. J. Biol. Chem. 272, 20011–20020. 20. Byler, D.M. & Susi, H. (1986) Examination of the secondary structure ... Characterization of PKI gamma, a novel isoform of the protein kinase inhibitor of cAMP- dependent protein kinase. J. Biol. Chem. 272, 18169–18178. 4. Gamm, D.M. & Uhler, M.D. (1995) Isoform-specific ... showedasinglebandbySDS/PAGE (Fig. 2). The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 10 4 unitÆmg )1 (Fig. 3A) .The K i value determined...

Ngày tải lên: 07/03/2014, 15:20

... effect on the thermal unfolding of proCPY. The P m value for CPY was higher than that of its unglycosylated form, though the DV and DG P values were almost the same. The P m value of proCPY was ... the corresponding values of proCPY and Dgly proCPY (Fig. 2B), indicating that the precursor form was less thermally stable than the mature form, regardless of the carbohydrate moiety. After the ... unfolding of the unglycosylated form. At high pressure, in the glycosylated forms the carbohy- drate moiety of CPY and proCPY would be hydrated to compensate the volume contraction and the protein...

Ngày tải lên: 07/03/2014, 21:20

... iden- tified as the major and the minor forms of the holo- ACP structure, on the basis of their percentage contri- butions (65% and 35%, respectively) to the overall structure of the protein. The structures ... both forms have the same amino acid sequence, and they have similar secondary structures, it was found that the – DG of unfolding of the holo form was lower than that of the apo form at all the ... unfolding thermodynamic parameters of the two forms are given in Tables 1–3. In the entire experimental regime, it is seen that the holo form presents better stability than the other form (Fig. 4C). The...

Ngày tải lên: 16/03/2014, 10:20

... true of unions of elements of the semilattice). These predicates are not only true of the conventional kinds but also of conventional servings such as the bottle of wine on the table or the ... speaking, the theories view the kinds of M as forming an upper semilattice of kinds with M at the top. This is a "formal" semilattiee in that the union of any two elements of it is ... for the two theories of mass terms. The p-rules are for the semantic occurrence approach while the s-rules are for the syntactic expression approach. The ontological underpinnings of these theories...

Ngày tải lên: 24/03/2014, 01:21

Ngày tải lên: 24/04/2014, 17:22

Ngày tải lên: 19/06/2014, 08:20

Ngày tải lên: 20/06/2014, 04:20

Ngày tải lên: 20/06/2014, 16:20

Ngày tải lên: 21/06/2014, 00:20

Ngày tải lên: 21/06/2014, 01:20