delivery of molecular beacons for live cell imaging and analysis of rna
Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... at this level of testing The decrease of cell positivity for KG-1 cells and rather constant results for K-562 cells can be judged as internal controls for cell- type specificity in the direct comparison ... galectins [24]; (b) tested as ligand for surface receptors of different types of tumor cells; and (c) injected into circulation with monitoring of the time course of biodistribution The data set ... instead of the neoglycoprotein and an inhibition of staining using glycoinhibitors (75 mM lactose and mg asialofetuinÆmL)1); (D) document lack of label ⁄ carrier protein-dependent binding and the...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... proteomics and chemical biology, namely protein microarray technologies for large-scale protein analysis and live cell bioimaging In the first application for live cell bioimaging, a protein of interest ... movement and interactions of proteins inside living cells is critical for a better understanding of cellular mechanisms and functions Traditionally this has been done by in vitro labeling of proteins ... in live cell imaging was demonstrated in visualizing the translocation of connexin in and out of gap junctions.[25] Non-covalent interactions of small molecule ligands with streptavidin- and...
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REDISCOVERING BIOLOGY - Molecular to Global Perspectives: Cell Biology and Cancer pot
... changes in cell structure, decreased cell adhesion, and production of new enzymes These heritable changes allow the cell and its progeny to divide and grow, even in the presence of normal cells that ... enough cells to fill the gap This characteristic is lost in cancer cells, which continue to grow after they touch other cells, causing a large mass of cells to form • Normal cells age and die, and ... factors and production of many of these is increased by a variety of oncogenes Therefore, oncogenes in some tumor cells allow those cells to produce angiogenic factors The progeny of these tumor cells...
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THEORY AND APPLICATIONS OF CT IMAGING AND ANALYSIS pdf
... to non-medical CT imaging and analysis such as for forensic and industrial applications The 16 chapters selected in this book cover not only the major topics of CT imaging and analysis in medical ... result was of the group 80/20, which obtained values of 90% of sensibility, 63.33% of specificity and 76.67% of accuracy Informatics and Computerized Tomography Aiding Detection and Diagnosis of Solitary ... (Chapters 10 to 14) Part 5: CT Imaging and Analysis for Non-Medical Applications (Chapters 15 and 16) Parts to are devoted to theories and applications of CT imaging and analysis in medical fields where...
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báo cáo khoa học: "Real-time imaging and analysis of differences in cadmium dynamics in rice cultivars (Oryza sativa) using positron-emitting 107Cd tracer" ppsx
... h for Koshihikari and 10.2 h for BIL48), the Cd transport velocities were calculated to be 6.0 cm h-1 for Koshihikari and 6.6 cm h-1 for BIL48 At the end of the PETIS experiment, the amount of ... the roots (pmol) and Cd in solution (pmol) in Figure indicate the sums of radioactive 107Cd and nonradioactive Cd Figure - Imaging and analysis of 107 Cd transport into shoots of six rice cultivars ... (Additional file 3), and the 109Cd ratio was counted Figure - Imaging and analysis of 107 Cd transport into the panicle for Koshihikari and BIL48 BIL48 carries the QTL responsible for high Cd accumulation...
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Báo cáo y học: " xGDB: open-source computational infrastructure for the integrated evaluation and analysis of genome features" pptx
... infrastructure for organization of genomic data, analysis of a wide range of inquiries about such data, and online publishing of both data and analysis results The extensible design of xGDB provides ... internal design and back-end components relevant to data providers and private installations The installation is straightforward and requires basic knowledge of common open source software For ... management scripts for loading features from flat files of various formats into a relational database management system, and supporting information necessary for feature configuration and cus- http://genomebiology.com/2006/7/11/R111...
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Development of an approach for interface pressure measurement and analysis for study of sitting
... work and puts forth recommendations and future work Development of an approach for interface pressure measurement and analysis for study of sitting CHAPTER Literature review 2.1 Applications of ... of an approach for interface pressure measurement and analysis for study of sitting The working principle of resistive sensor is the variation of resistance of a piezoresistive layer when a force ... Development of an approach for interface pressure measurement and analysis for study of sitting External sitting environment, including the ambient environment, supporting surface, and occupant’s internal...
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Level set methods for MRE image processing and analysis
... investigation, the modality for deformation or wave imaging, and the algorithm for elasticity interpretation In terms of motion imaging, both US and MRI have been proposed for elastography (Ophir ... Besides Prof Kobayashi, I have to thank Prof SAKUMAR Ichiro and Prof LIAO Hongen for their generous supports Ms OOBADA Naho, Ms OOKI Yusuko and Ms ISHIDA Yuko, the secretaries of BMPE, offered ... ns is the number of scales and nt is the number of directions; (ζω, ζθ) are the filter bandwidths for ω and θ respectively Figure 2-5 shows a group of lognormal Gabor filters for elasticity reconstruction...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... PCFC-g-PEI and FA-PEAs on Ana-1 and HUVEC cells was homoplastic with that on OS-RC-2 cells As shown in Figure 1C, the toxicity profiling of PCFC-g-PEI on HUVEC cells was similar with that on OS-RC-2 cells ... Because PCFC-g-PEI and FA-PEAs are amphiphilic polymers, both of them may form polymeric micelles through self-assembling During the process of the micelle formation, the concentrations of polymers...
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báo cáo hóa học:" Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" pot
... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... PCFC-g-PEI and FA-PEAs on Ana-1 and HUVEC cells was homoplastic with that on OS-RC-2 cells As shown in Figure 1C, the toxicity profiling of PCFC-g-PEI on HUVEC cells was similar with that on OS-RC-2 cells ... Because PCFC-g-PEI and FA-PEAs are amphiphilic polymers, both of them may form polymeric micelles through self-assembling During the process of the micelle formation, the concentrations of polymers...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers EJNMMI Research 2011 1:1 Submit your manuscript to a journal ... vivo studies KEB and KG carried out radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated ... absence of a tumor burden, the T1/2a and b for IgG was approximately 5.2 and 3.8 times faster, respectively, than in the presence of tumor In contrast, the T1/2a and b of the blood clearance for...
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Surface functionalization of superparamagnetic iron oxide nanoparticles for potential cell targeting, imaging, and cancer therapy applications
... OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2012 ACKNOWLEDGEMENTS My sincere and deep gratitude goes first and foremost ... spectra of (a) pristine SPIONs and (b) PLMA2-SPIONs. Figure 3-6 Schematic representation of the synthesis of PLMA-1-PEGSPIONs Site and extent of amide band formation between H2NPEG and PLMA-1 ... recognize and bind to the receptors that are upregulated on cancer cells but not expressed by normal cells Active targeting is particular useful for the delivery of drugs to reduce off-site toxicity and...
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Use of upconversion fluorescent nanoparticles for simultaneous imaging, detection and delivery of sirna
... the nanoparticles were also used for targeted delivery of siRNA into cells Besides monitoring its intracellular delivery process, the release and biostability of siRNA were also demonstrated based ... therapeutic agents, imaging contrast agents and targeting moieties The nanoparticle can be used for specific delivery of anticancer agents, tracking of therapeutic delivery, and detection of treatment ... nm between UCN and Oyster-556 dye Immunohistochemistry Detection of cell and tissue surface 200-400 nm antigens Cellular imaging Specific imaging of cancer cells In vivo Imaging Imaging in Caenorhabditis...
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Tài liệu Guidelines for the appointment of General Practitioners with Special Interests in the Delivery of Clinical Services pdf
... improvement in delivery of sexual health services Understanding of primary care structures and how these may affect delivery of services within the PCO Understanding of service redesign and care pathways ... treatment of common STIs Local management of sexual contacts and partner notification Epidemiology and prevalence of STIs Use and and Teenage contraindications of the pregnancy, and full range of other ... National, local and Professional strategies and guidelines and how these may relate to service configuration within the PCO Good understanding of roles, responsibility and structure of PCOs and how to...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... two intramolecular disulfide bonds, Cys12–Cys45 and Cys88–Cys298, which are required for hexamer assembly and structural stability [23–25] Folding and the formation of the disulfide bonds of glycinin ... region between b and b¢ in GmPDIL-1, and between b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant The activity of recombinant GmPDIL-1 and GmPDIL-2, which ... refolding of reduced and denatured RNaseA, was examined The specific activities of GmPDIL-1 and GmPDIL-2 were 472 and 300 mmol RNaseAÆmin)1Æmol)1, respectively (Fig 2A) Several mammalian and yeast...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
... valve and girdle band formation which are completed in less than h In future, analysis of cell division or valve formation in C fusiformis transformants carrying a gene of interest under control of ... 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introduced genes in transgenic C fusiformis Results Increasing the efficiency of C fusiformis transformation ... aliquots of cells were harvested every other hour for RT-PCR analysis and hourly for fluorescence measurements The same type of kinetic analysis was performed in a second experiment, in which cells...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... of normal cellular events such as cell cycle and apoptosis, RNA interference studies were performed using VBARP siRNA and control siRNA Several VBARP siRNA duplexes were synthesized and purified ... the lipofectamine control Different concentrations of VBARP and control siRNA used are indicated at the bottom of the respective lanes (B) Effect of siRNA on cell viability HeLa1 and NT22 cells ... tissues and human primary cells using Qiagen RNA purification kit (Qiagen, Valencia, CA) and used in real-time PCR analysis for various VBARP isoforms Two-step RT-PCR was performed as follows: RNA...
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Báo cáo khoa học: Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx
... Specificities of reactivating factors in the complex formation with dehydratases DDR and GDR form a complex with apoenzymes of diol and glycerol dehydratases, respectively, and transient formation of such ... dimension and SDS ⁄ PAGE in the second) The analysis provided all of the a, b, and c subunits of glycerol dehydratase and the a and b subunits of DDR (Fig 5C), indicating that this new band corresponds ... (DR), and GDR (GR) are indicated with arrowheads to the right of the gels, and their complexes 1–4 to the left Bands and correspond to : and : DD–DDR complexes, respectively, and bands and to...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... tool for the optimization of GPCR expression conditions, for the localization of ligand-binding sites, and for the identification of compounds with new properties that could be important for the ... Glaubitz and Andreas Engel for valuable discussions, and we thank Walter Rosenthal for the cDNA of human ETB We further ´ thank Robert Tampe and Katrin Schulze for their help with SPR analysis ... cHx cHx cHx A B Fig PAGE analysis of CF-expressed ETB fragments on SDS gels (A) CF expression of ETB fragments in the presence of Brij78; 0.7 lL of supernatant (S) and lL of eluate (E) after Ni–chelate...
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Báo cáo khoa học: Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells ) implication for placental cell fusion pot
... glycosylated form of CD98 A band of lower molecular mass ( 53 kDa) was present in both control and forskolin-stimulated cell lysates after tunicamycin treatment (A2), with an additional band of 49 ... the context of cellular distribution and cellular fusion Results Expression of CD98 increases over time almost linearly after forskolin treatment Fusion of BeWo cells is enhanced by forskolin treatment, ... necessary for virus-induced cell fusion and for osteoclast formation [10–12] and, importantly, it is found in cytotrophoblasts and on the plasma membrane of the syncytiotrophoblast of the human...
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