biochemistry of cytosolic sulfotransferases involved in bioactivation
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig 2) PCR reactions performed in ... domain Taking into account the important role of Ptas, the results obtained in the present work, dissecting the different domains forming E coli Pta, will help in the future manipulation of these...
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Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide ppt
... orientation of submitted sequences Within lgt3 Within lgt2 Within lgt1 R R F Within Within Within Within Within F R R F R lgt5 lgt2 lgt1 lgt22951 lgt4 Within lgt1 Within lgt4 Within lgt4 Within lgt4 ... irradiating each of the anomeric signals in turn to obtain the H chemicals shifts of the corresponding remaining ring protons Again, assignment of the Kdo residue was possible from examination of ... Transformation of strain 2951 resulted in recombination within lgt5 and lgt1, resulting in deletion of lgt4 and disruption of lgt1 (C) Construction of plasmid for mutation of lgt5 and deletion of lgt4:...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... displaying two putative splice variants [Fig 2] Expression of fucokinase cDNAs The two splice variants of fucokinase were subcloned into the XbaI site of a pQM vector containing a C-terminal E2-Tag/A ... variants of L-fucokinase The long splice variant of L-fucokinase consisted of 3270 bp, encoding a protein of 1090 amino acids The sequence of the shorter cDNA was similar to the sequence of the IMAGE ... alternative splicing maintains the reading frame along the entire protein, therefore the protein variants are identical in the amino-terminal end up to the alternative splice area, in addition to...
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot
... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of ... provide insight regarding the mechanism, the substrate binding site and the role of the metal cofactor in the enzyme Results Cloning, expression and purification of BphH The bphH gene was inserted into ... p.p.m Extinction coefficients of HPDA and substituted HPDA were determined by adding XylE and TodF stepwise to solutions containing known amounts of catechol and determining the resulting UV absorbance...
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báo cáo khoa học: " Connectedness of healthcare professionals involved in the treatment of patients with Parkinson’s disease: a social networks study" pot
... prominence and influence in the network The aim of this study was to examine the connectedness in a newly established regional ParkinsonNet of health professionals involved in the treatment of ... person was involved in professional contacts so far Knowing each other was defined in the questionnaire as ‘knowing the face, having talked to each with other, or having heard of. ’ Having professional ... reach in a specific number of steps in the network of in/ outgoing connections Betweenness centrality Number of pathways in the network in which an individual is in between’ of two other individuals...
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báo cáo khoa học: " Is the basal area of maize internodes involved in borer resistance?" docx
... damage in the area AExamples of damage caused by larval feeding in internode basal area B - Length of the basal internode ring (LIBR) LIBR measurement on one side of the internode (arrow in red), ... During development, internodal cells undergo rapid elongation and the pulvinus line develops from the remains of the intercalary meristem at the base of the internode [22] The trait recorded in ... consisted of acetotrinile (Solvent A) and trifluoroacetic acid (0.05%) in water (solvent B) delivered in the following gradient conditions: initial A: B ratio of 10:90, changing to 30:70 in 3.5 min,...
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báo cáo khoa học: " DNA polymorphisms and haplotype patterns of transcription factors involved in barley endosperm development are associated with key agronomic traits" pot
... (GAMYB), the barley leucine zippers and (BLZ1, BLZ2), and the barley prolamin box binding factor Page of 11 (BPBF) that were shown to be involved in the transcription of B-hordeins encoded by the ... subgroup indicating footprints of selection on BLZ1, BLZ2 and BPBF However, selection might act in different ways: In case of BLZ2 selection resulted in the accumulation of a large number of low ... However, individual r2 values sharply decreased to r2
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báo cáo khoa học: " Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis" pps
... stress per se In another study the expression of 150 genes in response to wounding and insect feeding was carried out [58], finding that some of the responding genes, for which the inducing stimulus ... stresses in sunflower Among them, many genes potentially encoding components involved in photosynthesis, such as photosystem proteins, chlorophyllbinding proteins, Rubisco and light harvesting proteins ... protein specifically induced under chilling treatment The large amount of TFs identified in Arabidopsis, indicating the complexity in the secondary metabolism of the plants [82], could explain...
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Báo cáo y học: "Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain" potx
... through binding of two LXXLL motifs in the activation domain of the viral protein to the KIX domain of the coactivators [47] Mutating both LXXLL motifs, which severely diminishes binding to p300/CBP, ... transcription in an uninfected and an HTLV-1-infected T-cell line We were interested in determining whether the expression of HBZ alone induces DKK1 gene expression in T-cells, and whether the increased ... the expression of genes involved in osteoblast differentiation and activation [24] Dkk1 inhibits this process by binding to LRP5/6, which competitively inhibits binding by Wnt proteins [24] Additionally,...
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Báo cáo y học: " Patterns of evolution of host proteins involved in retroviral pathogenesis" potx
... periods would have resulted in signatures of positive selection indicative of a genetic conflict Finally, the absence of a signature of positive Darwinian selection in Cyclophilin A provides a complement ... domain [21] The protein Vif, which counteracts the activity of APOBEC3G, is encoded by nearly all lentiviruses [22] Within the Vif-interaction domain of APOBEC3G, 10 residues can be pinpointed ... isomerase), is incorporated into HIV-1 particles through an interaction with the viral capsid [10] Cyclophilin A is incorporated only into viral particles of viruses of the HIV-1M/SIVCPZ lineage, where...
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Single DNA studies of architectural proteins involved in bacterial pathogenesis and meiosis in saccharomyces cerevisiae
... protein molecules and their interactions with DNA in real-time reactions and the dynamics of their DNA-binding Therefore, my objective of finding the characteristics of protein binding to single ... HU bind to DNA non-cooperatively causing DNA bending (20) at low protein binding density While at high protein binding density, IHF can crosslink DNA causing DNA condensation in the presence of ... is an NAP constituting 7–10% of the total protein in Mtb (78) It consists of 205 amino acid residues and contains large amounts of alanine (23.78% of total amino acids) and lysine (18.93%), with...
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A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
... Follistatin (FS) is a glycoprotein structurally unrelated to the activins and inhibins Due to its high binding affinity to activin, FS binds to and neutralizes the bioactivity of activin on the ... C domain is the DNA-binding domain, which consists of two zinc-finger-like motifs The D domain is a variable hinge The multifunctional C-terminal (domain E) is the ligand-dependent domain (LBD), ... Ubc4 over-expression increases the interaction of ERα with Sf-1or Pitx1 84 v 3.3 Calcineurin is involved in the GnRH activation of the αGSU gene promoter 86 3.3.1 Calcineurin catalytic subunit...
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Structural and functional analysis of critical proteins involved in mRNA decay
... structure of the interacting domains of hUpf2 and hUpf3b shows that the RNA-binding domain (RBD) of Upf3 is involved in the interaction with one MIF4G domain of Upf2, rather than RNA binding (Kadlec ... include the poly(A)-binding protein (Pab1) and the cap-binding protein (eIF4E) Pab1 couples decapping with deadenylation and inhibits decapping by promoting the formation of the translation initiation ... eRF3 contains at least two functional regions: an N-terminal region, which is not necessary for translation termination but is involved in binding to poly(A)-binding protein, suggesting a link between...
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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
... are involved in the early development of chronic pain, while astrocytes function in sustaining the pain (Vallejo et al., 2010) An interesting finding was that nerve injury induces an increase in ... Peroxidase xiv IL-1 Interleukin-1 IL-1β Interleukin-1β IL-6 Interleukin-6 IL-8 Interleukin-8 IL-10 Interleukin-10 IL-18 Interleukin-18 i.t Intrathecal L-PGDS Lipocalin-type Prostaglandin D Synthase ... 2007) Amino acids including aspartate, arginine, asparagine, tyrosine and valine were also demonstrated to be elevated in the CSF of chronic pain patients as compared to that of acute pain patients...
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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... change in the pKa value of the amino acid residue(s) involved in malate inhibition may occur as a result of interaction with different amino acid residues in the allosteric site The amino acid ... structure of each isoenzyme that are involved in fumarate activation (in yellow), CoA activation (underlined) and malate inhibition (in green) of the forward reaction are highlighted In addition, ... regions involved in the differential regulation by fumarate and succinate could not be mapped by this approach Moreover, the mutation of R115 in NADPME2 indicated that this amino acid residue is involved...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray results ... ANK3 Gene title cofactor of BRCA1 integrin, beta staufen, RNA binding protein, homolog (Drosophila) high mobility group nucleosomal binding domain ankyrin 3, node of Ranvier (ankyrin G) Signal Log...
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Risks Involved in the Use of Herbal Products
... this kind of information are found in Ody (1993) and Moore (1995) 14.2 Inadequate Testing of Herbal Medicine Products Many medicinal herbs have not yet been subjected to testing in human clinical ... to inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen radical-forming ... may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify the side effects of...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... suggesting that the heterologous expression of genes encoding metalloproteins can lead to incorrect metal ion incorporation This is observed in rubredoxin and desulforedoxins where zinc, instead of ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature ... not contain NrdI, as assessed by ESI-QTOF-MS In our view, C ammoniagenes restricts the incorporation of iron into R2F in vivo, even in the absence of manganese, and it is the availability of manganese...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimerization [43] Each of these...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a manner similar ... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment revealed an ORF showing amino acid ... transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25