basic fibroblast growth factor

Báo cáo hóa học: " In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release" ppt

Ngày tải lên : 22/06/2014, 00:20

... that this new delivery system can help tissue regeneration and wound healing by releasing the growth factors in a controlled and temporal manner Base a longer release period, around weeks [13] ... hydrolysis and condensation reactions Basic FGF is a protein with a length of 155 amino acids and an isoelectric point of 9.6, which makes it stable in a weak basic solution Therefore, NH4OH as ... internalization of MSNs, indicating that the MSNs are not only a biocompatible delivery system for growth factors/ proteins, but also an ideal biomaterial for the application in bio-imaging Conclusions...

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báo cáo khoa học: " The impact of hyperbaric oxygen therapy on serological values of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)" pptx

Ngày tải lên : 11/08/2014, 20:20

... endothelial growth factor (VEGF) and basic growth factor (bFGF) In angiogenesis, a number of growth factors (for example Angiopoietin-1, Platelet derived growth factor, Hypoxia inducing factor- 1a, ... endothelial growth factor J Biol Chem 1994, 269:26988-95 20 Dietz A, Rudat V, Vanselow B, Conradt C, Möhler T: [Predictive value of serum levels of basic fibroblast growth factor, vascular endothelial growth ... endothelial growth factor and basic fibroblast growth factor on angiogenesis in vivo Circulation 1995, 92:II365-71 25 Salven P, Mänpää H, Orpana A, Alitalo K, Joensuu H: Serum vascular endothelial growth...

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Expression of basic fibroblast growth factor during periodontal healing of replanted dogs teeth

Ngày tải lên : 05/10/2015, 22:31

... 27 2.5.5 Growth Factors and Periodontal Healing 28 2.6 Fibroblast Growth Factor 31 2.6.1 Types of Fibroblast Growth Factor 32 2.6.2 Fibroblast Growth Factor Receptors ... acidic Fibroblast Growth Factor bFGF/FGF-2 - basic Fibroblast Growth Factor BMP - Bone Morphogenetic Protein CEC - Capillary Endothelial Factor ECM - Extracellular Matrix EGF - Epidermal Growth Factor ... Factor FGF - Fibroblast Growth Factor FGFR - Fibroblast Growth Factor Receptor HSPG - Heparan Sulfate Proteoglycan IGF - Insulin-like Growth Factor PDGF - Platelet Derived Growth Factor PDL -...

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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

Ngày tải lên : 21/02/2014, 03:20

... growth factor- 2 Endrocrin Rev 1, 26±45 Laslett, A.L., McFarlane, J.R., Hearn, M.T.W & Risbridger, G.P (1995) Requirement for heparan sulphate proteoglycans to mediate basic ®broblast growth factor ... Meroni, S.B., Capena, D.F., Pellizzari, E.H & Cigorraga, S.B (1999) Eects of basic ®broblast growth factor and nerve growth factor on lactate production, c-glutamyl transpeptidase and aromatase activities ... 276±288 44 Romaris, M., Bassols, A & David, G (1995) Eect of transforming growth factor- b1 and basic ®broblast growth factor on the expression of cell surface proteoglycans in human lung ®broblasts...

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Báo cáo sinh học: " Fibroblast growth factor 2 orchestrates angiogenic networking in non-GIST STS patients" potx

Ngày tải lên : 18/06/2014, 19:20

... Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; NR, not reached; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor; ... VEGFR-3 Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor (B) FGF2 uPa ... extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth factor receptor; MMP, matrixmetallo...

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o cáo hóa học:" Fibroblast growth factor 2 orchestrates angiogenic networking in non-GIST STS patients" pdf

Ngày tải lên : 20/06/2014, 04:20

... Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; NR, not reached; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor; ... VEGFR-3 Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor (B) FGF2 uPa ... extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth factor receptor; MMP, matrixmetallo...

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Báo cáo y học: "The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia" pdf

Ngày tải lên : 09/08/2014, 07:20

... normoxia; (c) hypoxia) and presence ((b) normoxia; (d) hypoxia) of fibroblast growth factor (FGF2) Scale bar, 100 µm FGF2, fibroblast growth factor specific type II collagen gene promoter activity [35] ... tension (n = 3) *p < 0.05; **p < 0.001 (Student's unpaired t test) in cell aggregates from fibroblast growth factor (FGF2)-expanded versus non-FGF2-expanded cells P2, passage 2; P3, passage AGCTTCTGTGGAACCATGGAA-3' ... under hypoxia after 14 days in culture AGG, aggrecan; BGN, biglycan; DCN, decorin; FGF2, fibroblast growth factor 2; FMOD, fibromodulin; P2, passage 2; P3, passage Page of (page number not for citation...

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Báo cáo y học: "Action of fibroblast growth factor-2 on the intervertebral disc" docx

Ngày tải lên : 09/08/2014, 10:23

... proinflammatory cytokines and growth factors such as IL-1 [5,11,12] – and potentially fibroblast growth factor (FGF2) (otherwise known as basic fibroblast growth factor) [13] – both of which have ... EO: Growth factor expression in degenerated intervertebral disc tissue An immunohistochemical analysis of transforming growth factor beta, fibroblast growth factor and platelet-derived growth factor ... 25:1228-1238 Posever J, Phillips FM, Pottenger LA: Effects of basic fibroblast growth factor, transforming growth factor- beta 1, insulin-like growth factor- 1, and insulin on human osteoarthritic articular...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... present in the IPFP tissue of fibroblast growth factor- 2 (FGF-2) expansion Chondrogenic cultures of infrapatellar fat pad (IPFP) cells and effects of fibroblast growth factor- 2 (FGF-2) expansion ... ascorbate 2-phosphate, 100 nM dexamethasone, 10 ng/mL transforming growth factor (TGF)-β3, and 100 ng/mL insulinlike growth factor- 1 (all from Sigma-Aldrich) Gene expression analysis Quantitative ... markers on freshly isolated bone marrow-derived Figure ation rates and morphology Effects of fibroblast growth factor- 2 (FGF-2) expansion on cell proliferation rates and morphology (a) Cell proliferation...

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Báo cáo y học: "Role of fibroblast growth factor 8 (FGF8) in animal models of osteoarthritis" doc

Ngày tải lên : 09/08/2014, 10:23

... signaling is transduced through the formation of a complex of a growth factor, a proteoglycan, and a high-affinity fibroblast growth factor receptor (FGFR), which is a transmembrane tyrosine kinase ... Arthritis Research & Therapy Vol 10 No Uchii et al Fibroblast growth factor (FGF) is a family of at least 24 growthregulatory proteins – sharing 35% to 50% amino acid sequence ... osteoarthritis Ann Rheum Dis 2005, 64:694-698 Szebenyi G, Fallon JF: Fibroblast growth factors as multifunctional signaling factors Int Rev Cytol 1999, 185:45-106 Draper BW, Stock DW, Kimmel CB:...

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Báo cáo y học: "Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes" pptx

Ngày tải lên : 12/08/2014, 17:22

... Fibroblast growth factor receptor is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular ... 44:585-594 Loeser RF, Chubinskaya S, Pacione C, Im HJ: Basic fibroblast growth factor inhibits the anabolic activity of insulin-like growth factor and osteogenic protein in adult human articular ... Sah RL, Trippel SB, Grodzinsky AJ: Differential effects of serum, insulin-like growth factor- I, and fibroblast growth factor- 2 on the maintenance of cartilage physical properties during long-term...

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Báo cáo sinh học: "Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic mice" pptx

Ngày tải lên : 14/08/2014, 19:22

... effects of fibroblast growth factor- 4 in endothelial cells Oncogene 2001, 20(21):2655-2663 Aviezer D, Safran M, Yayon A: Heparin differentially regulates the interaction of fibroblast growth factor- 4 ... capabilities of fibroblasts using recombinant growth factors Proliferation of diabetic and wild-type fibroblasts was measured using BrdU incorporation assay (Figure 7a) Diabetic fibroblasts cultured ... tyrosine kinase-1 receptor (VEGF receptor 1); FGF4: fibroblast growth factor 4; MMP-9: matrix metalloproteinase-9; VEGF-A: vascular endothelial growth factor- A Acknowledgements Grzegorz Dyduch, PhD,...

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Tạo dòng, biểu hiện và tinh chế hFGF2 (FIBROBLAST GROWTH FACTOR2) tái tổ hợp từ Escherichia coli

Ngày tải lên : 27/09/2014, 15:27

... thích tế bào nội mô phân bào với khối lƣợng phân tử 13-18kDa, có lực cao với heparin điểm đẳng điện basic Những chất kích thích phân bào bao gồm nhân tố tăng trƣởng tế bào hình sao, nhân tố tăng trƣởng ... Phenol bão hòa TE (pH 8,0) - Hóa chất dùng cho tinh chế DNA - Bộ kit tinh chế EZ-10 spin column (Biobasic) e) Hóa chất dùng cho biến nạp DNA plasmid vào E coli - Dung dịch CaCl2 100mM vô trùng - Dung ... 4oC) - Dung dịch glycerol 80% vô trùng f) Hóa chất dùng cho điện di DNA gel agarose - Agarose (Biobasic) - Dung dịch chạy điện di TAE 50X: Tris acetate 40mM, EDTA 0,1M (pH 8,0) - Dung dịch nạp mẫu...

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Nghiên cứu sản xuất protein fgt 2 (fibroblast growth factor 2) tái tổ hợp trong vi khuẩn escherichia coli

Ngày tải lên : 08/10/2014, 18:16

... Bovin Serum fgf Fibroblast Growth Factor Gene FGF Fibroblast Growth Factor FGFR Fibroblast Growth Factor Receptor HRP Horseradish peroxidase iFGF Intracellular Fibroblast Growth Factor IPTG Isopropyl-β-D-thio-galactoside ... -1- LỜI MỞ ĐẦU Nhân tố tăng trƣởng nguyên bào sợi FGF-2 (Fibroblast Growth Factor- 2) protein đa chức năng, tham gia điều hòa hoạt động nhiều trình thể nhƣ cân nội ... tăng cƣờng tính tan protein tái tổ hợp Luận văn thạc sĩ sinh học - 20 - - Bổ sung cofactor: Việc thêm vào cofactor đem đến hiệu khả quan việc làm tăng tính tan protein Nhiều nghiên cứu cho thấy,...

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TẠO DÒNG, BIỂU HIỆN và TINH CHẾ HFGF 2 (FIBROBLAST GROWTH FACTOR 2) tái tổ hợp từ ESCHERICHIA COLI 3

Ngày tải lên : 26/11/2015, 08:21

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TẠO DÒNG, BIỂU HIỆN và TINH CHẾ HFGF 2 (FIBROBLAST GROWTH FACTOR 2) tái tổ hợp từ ESCHERICHIA COLI 4

Ngày tải lên : 26/11/2015, 08:21

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TẠO DÒNG, BIỂU HIỆN và TINH CHẾ HFGF 2 (FIBROBLAST GROWTH FACTOR 2) tái tổ hợp từ ESCHERICHIA COLI 1

Ngày tải lên : 26/11/2015, 08:21

... Tổng quan tài liệu 1.1 HỌ NHÂN TỐ TĂNG TRƢỞNG NGUYÊN BÀO SỢI FGF (FIBROBLAST GROWTH FACTOR) 1.1.1 Giới thiệu chung Nhân tố tăng trƣởng nguyên bào sợi FGF đƣợc Armelin tìm ... tăng sinh nguyên bào sợi Sau đó, Gospodarowicz tinh chế phân đoạn dịch chiết đƣợc tìm thấy pH acid basic Thí nghiệm cô lập đƣợc hai dạng FGF khác tƣơng ứng với phân đoạn pH Nhân tố tăng trƣởng thu ... thích tế bào nội mô phân bào với khối lƣợng phân tử 13-18kDa, có lực cao với heparin điểm đẳng điện basic Những chất kích thích phân bào bao gồm nhân tố tăng trƣởng tế bào hình sao, nhân tố tăng trƣởng...

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TẠO DÒNG, BIỂU HIỆN và TINH CHẾ HFGF 2 (FIBROBLAST GROWTH FACTOR 2) tái tổ hợp từ ESCHERICHIA COLI 2

Ngày tải lên : 26/11/2015, 08:21

... Phenol bão hòa TE (pH 8,0) - Hóa chất dùng cho tinh chế DNA - Bộ kit tinh chế EZ-10 spin column (Biobasic) e) Hóa chất dùng cho biến nạp DNA plasmid vào E coli - Dung dịch CaCl2 100mM vô trùng - Dung ... 4oC) - Dung dịch glycerol 80% vô trùng f) Hóa chất dùng cho điện di DNA gel agarose - Agarose (Biobasic) - Dung dịch chạy điện di TAE 50X: Tris acetate 40mM, EDTA 0,1M (pH 8,0) - Dung dịch nạp mẫu ... NaCl 2M, pH - Dung dịch Ethanol 20% - Nƣớc cất j) Một số hóa chất khác - Ampicillin 100mg/ml (Biobasic) (giữ -30oC) - IPTG (Isopropyl1-β-D-thiogalactoside) 1M (giữ -30oC) - Các chất điều chỉnh...

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Tạo dòng, biểu hiện và tinh chế hFGF 2 (FIBROBLAST GROWTH FACTOR 2) tái tổ hợp từ escherichia coli

Ngày tải lên : 26/11/2015, 08:50

... 25 PADERA, R F (2000) Cell signaling by fibroblast growth factor 2: investigations into interactions between fibroblast growth factor 2, fibroblast growth factor receptor and heparin-like glycosaminoglycans ... of fibroblast growth factorfibroblast growth factor receptorheparin-like glycosaminoglycan complex The National Academy of Sciences 33 WANG, D I K (2000) The effects of fibroblast growth factor ... AL., (1997) Biological Roles of Fibroblast Growth Factor- 2, Endocrine Reviews, vol 18, no 1, pp 26-45 A LOGAN, A BAIRD, (1996) "Fibroblast growth factors," Growth Factors and Cytokines in Health...

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Tài liệu HEMATOPOIETIC GROWTH FACTORS IN ONCOLOGY BASIC SCIENCE AND CLINICAL THERAPEUTICS pptx

Ngày tải lên : 16/02/2014, 07:20

... Hematopoietic growth factors Therapeutic use Hematopoietic growth factors Mechanism of action Cancer-Chemotherapy [DNLM: Hematopoietic Cell Growth Factors therapeutic use Hematopoietic Cell Growth Factors ... on growth factors In cultures, these growth factors were provided by serum, conditioned medium, or cell underlayers The growth factors present in these sources were called colony-stimulating factors ... INTRODUCTION ANIMAL MODELS OF HEMATOPOIETIC GROWTH FACTOR DEFICIENCY ANIMAL MODELS OF HEMATOPOIETIC GROWTH FACTOR EXCESS ANIMAL MODELS OF HEMATOPOIETIC GROWTH FACTOR ADMINISTRATION AFTER CHEMOTHERAPY...

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