d fibroblast growth factor 23 and alkaline phosphatase

... COL2A1 and SOX9 was modulated further by culture at low oxygen tension In nonFGF2-expanded cells COL2A1 increased 60-fold and SOX9 12-fold, whereas COL2A1 increased 80,000-fold and SOX9 80-fold in ... expression of decorin was low and remained unchanged between cell aggregates formed from FGF2expanded and non-FGF2-expanded cells, but the expression of decorin was 3-fold to 6-fold lower in cell ... antibody control on cell aggregates derived from non-FGF2-expanded and FGF2expanded cells after culture under hypoxia Scale bar, 100 µm expanded cells at low oxygen tension Although this did not...

Ngày tải lên: 09/08/2014, 07:20

... infrapatellar fat pad-derived cells expanded in normal medium (black bars) and FGF-2-supplemented medium (white bars) at days 2, 4, 6, 8, and 10 are shown Data are mean ± standard error of the mean ... antibodies and fluorescein isothiocyanate-conjugated secondary antibody (green) and DAPI (4'-6-diamidino-2-phenylindole) (blue) Results showed strong staining for CD13, CD29, CD44, CD90, and CD105, ... for 14 days in IPFP cells expanded with and without fibroblast growth factor- 2 (FGF-2)-supplemented medium is shown analyses showed that the IPFP tissue was well vascularised and 3G5 stained cells...

Ngày tải lên: 09/08/2014, 10:23

... and drafted the manuscript, KW and SJ participated in the design of the study and performed the statistical analysis HP and JK conceived of the study, and participated in its design and coordination ... years - Distribution of sex and age in the study and the control group (SD = standard deviation) Statistical analysis The results are expressed as mean values +/- standard deviations (SD) for ... of distribution of results, means and SD were controlled with the David, Pearson and Stephens test Data were statistically analyzed by using the analysis of variance (ANOVA) and Scheffe test Differences...

Ngày tải lên: 11/08/2014, 20:20

... SYH and AJ participated in design of the study and helped to draft the manuscript JD conceived of the study, designed it and edited the manuscript All authors read and approved the final manuscript ... the underlying muscle layer were harvested and fixed in 10% neutral buffered formalin for at least 24 h at room temperature, dehydrated in graded ethanol, cleared in xylene and embedded in paraffin ... participated in the design of the study, carried out the practical work and drafted the manuscript PK, JL, AZ, AS, JS, MK, HT, TO, RD and EV participated in the practical work and discussions SYH and...

Ngày tải lên: 14/08/2014, 19:22

... in the study design, result interpretation and in the writing TK, AV, SS and ES contributed in making the clinical and demographic database TK, SS and AV scored the cores TK and TD did the statistical ... formed vessle; Abbreviations: ECM, extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth ... recruitment of endothelial cells and increasing release of MMPs and uPa leading to ECM degradation and remodeling thus enabling tumor cell expansion and motility, PDGF-B recruiting VSMCs and stimulating...

Ngày tải lên: 18/06/2014, 19:20

... indicated as mean ± standard deviation (SD) † Unpaired, independent 2-tailed student t test analysis showed that mean age differ significantly among the groups (p = 0.004) ‡ Duration of disease ... VEGF-A (A) and CCL2 (B) in PBMCs of ALS patients Values are plotted as mean ± SE (Standard error) in the bar diagram Data was analyzed by unpaired, independent 2-tailed student t test # indicates ... born in North India and diagnosed with ALS were included from a convenience sample of Neurology outpatient, post graduate institute of medical education and research (PGIMER), Chandigarh after obtaining...

Ngày tải lên: 19/06/2014, 22:20

... in the study design, result interpretation and in the writing TK, AV, SS and ES contributed in making the clinical and demographic database TK, SS and AV scored the cores TK and TD did the statistical ... formed vessle; Abbreviations: ECM, extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth ... recruitment of endothelial cells and increasing release of MMPs and uPa leading to ECM degradation and remodeling thus enabling tumor cell expansion and motility, PDGF-B recruiting VSMCs and stimulating...

Ngày tải lên: 20/06/2014, 04:20

... acid route leads to a soft network because the hydrolysis is catalyzed easily compared to the condensation [23] , while the alkaline route is favoured to both hydrolysis and condensation, and produces ... of 96-well dish were plated and allowed to adhere overnight The medium was changed and replaced with fresh medium containing MSNs in next day At specific time points, cytotoxicity and viability ... g-1), tunable pore sizes (2–10 nm in diameter), and well-defined surface properties [17] In addition, MSNs have been approved by the Food and Drug Administration (FDA) as a new biocompatible material...

Ngày tải lên: 22/06/2014, 00:20

... study, and participated in the coordination of the study and writing of the manuscript IB conceived the study, selected the patients for study, participated in its design and coordination, and ... blood was drawn for laboratory studies Specifically, antinuclear antibodies (ANAs), antibodies to double stranded (ds)DNA and anticardiolipin (ACL) were measured ANAs were measured by indirect ... manuscript PB conceived the study, participated in its design and coordination and data analysis and writing of the manuscript All authors read and approved the final manuscript Acknowledgements The authors...

Ngày tải lên: 09/08/2014, 08:22

... reduction could be due to either increased PG degradation or decreased synthesis, or due to both Examples of increased PG degradation include the FGF2-stimulated, MMP-13-mediated or ADAMTS4-mediated ... analysis and interpretation of the data ME participated in analysis and interpretation of the data, and manuscript preparation EJT participated in the study design and manuscript preparation DKP and ... incorporation and DMMB results suggest that, per cell, PG synthesis and total PG accumulation decreased dose dependently after treatment with FGF2 In addition, Tsai and colleagues reported increased gene...

Ngày tải lên: 09/08/2014, 10:23

... localisation of CD44, CD29, CD105 and CD90, but no localisation of haematopoietic markers CD45, CD34 [23] These sand rat-derived AD-MSC are positive for the markers CD29 and CD105 and negative for ... in its design and co-ordination HT and HEG wrote the manuscript HT, MK and RD performed all experiments and assays HT and RD retrieved tissues from animals JAI performed and modified all immunohistochemical ... markers CD45 and CD34 The readily available antihuman markers CD90 and CD44 did not cross-react with sand rat tissue; thus they were not tested in the present study Since anti-sand rat antibodies...

Ngày tải lên: 09/08/2014, 10:23

... mesodermal and ectodermal origin [7,8] FGF8 was originally isolated from the conditioned medium of an androgen-dependent mouse mammary tumor cell line (SC3) as an androgen-induced growth factor, ... original idea for the study The experimental study was designed and carried out by MU, TT, and TS Pathological analysis was performed by MK AT provided information on FGF8 and reviewed the studies ... antibody not only reduced cartilage degradation induced by the injection of FGF8 in the joints, but also decreased cartilage degradation in the MIA-induced rat arthritis model These data indicate...

Ngày tải lên: 09/08/2014, 10:23

... creatinine and pg/mg of creatinine, respectively At the conclusion of the study, harvested bladders were homogenized, lysed, and stored at -80°C All tissue TGF-β1 values were then standardized by bladder ... involved in data analysis and manuscript preparation All authors read and approved the final manuscript Acknowledgements The work in this study was supported by NIH grant NIDDK RO1-DK 066138 and NIDRR ... TGF-β1 by ELISA, and levels of TGF-β1 were then standardized by bladder weight and expressed as μg per bladder Male rats exhibited the highest expression of TGF-β1 in tissue compared to tissue levels...

Ngày tải lên: 11/08/2014, 08:22

... that ADAM17 is required for both IL-13-induced proliferation and TGFα shedding in these cells This conclusion is supported by data demonstrating that the proliferation and growth factor shedding ... BWB performed the majority of the studies, participated in study design and data interpretation, and drafted the manuscript TS prepared RNA samples as well as performed and interpreted the RT-PCRs ... cells were exposed to control media for 60 min, reacted with fluorescent-tagged antibodies against TGFα (red) and ADAM17 (green), as described in Materials and methods, and then imaged by confocal...

Ngày tải lên: 12/08/2014, 15:20

... analysis and drafted the manuscript DC and H-JI designed the experiments of this study, interpreted the data and drafted the manuscript SC and GM interpreted the data KM interpreted the data, carried ... isolated cells were washed and suspended in culture media at × 106 cells/ml, and seeded onto 12-well plates using ml media/well Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 ... interpreted the data and drafted the manuscript All authors read, edited, and approved the final manuscript Acknowledgments We would like to thank the tissue donors, Drs Gabriella Cs-Szabo, Arkady Margulis,...

Ngày tải lên: 12/08/2014, 17:22

... link cytokines and the GH–IGF-I axis Authors' contributions MJO'L and RCS conceived of and designed the study All authors participated in the animal handling and procedures CJS carried out the immunoassays ... volumes of hydrogen peroxide and the chromagen tetramethylbenzidine) was added and incubated for a further 30 minutes at room temperature Finally, 100 μl stop solution (HCl) was added and the absorbance ... immunoassays and AX performed the PCR studies MJO'L performed the statistical analysis MJO'L, AX and CJS helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements...

Ngày tải lên: 13/08/2014, 08:20

... weight dH2O Distilled water (nƣớc cất) DMEM Dulbecco’s Modified Eagle’s Medium DMEM/F12 Dulbecco’s Modifed Eagle’s Medium and Ham’s F12 medium DNA Deoxyribose Nucleic Acid dNTP DeoxyNucleotide TriPhosphate ... TriPhosphate DTT Dithioerythritol E coli Escherichia coli EDTA Ethylene Diamine Tetraacetic acid FBS Fetal Bovin Serum fgf Fibroblast Growth Factor Gene FGF Fibroblast Growth Factor FGFR Fibroblast Growth ... Ribose Nucleic Acid RNase Enzyme thuỷ giải RNA rpm Round Per Minute SDS Sodium Dodecyl Sulphate SDS-PAGE Sodium Dodecyl Sulphate - Polyacrylamide Gel TEMED Tetramethylethylenediamine Luận văn...

Ngày tải lên: 08/10/2014, 18:16

... platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and growth hormone were differentially expressed according to the stage of development, morphogenesis, and cell differentiation ... extracted endodontically treated dog’s teeth were stored in ViaSpan for hours and replanted into 6, 48 or 96 hours sockets Controls included teeth extracted and replanted immediately, 48 hour stored ... these studies, the observational periods of ½ day, days, days and days were designed 25 2.5 Growth Factors 2.5.1 General Information Growth factors can be defined as signaling peptides that act...

Ngày tải lên: 05/10/2015, 22:31

... 40%, 60%, 80%, 100% dung d ch dung ly B Kết điện di SDS-PAGE đƣợc trình bày hình 3.12 cho thấy phân đoạn 20% dung d ch B xuất vạch protein nằm vạch có kích thƣớc 14,4kDa 20,1kDa thang chuẩn, phù ... lƣợc tạo d ng đầu d nh Kết thu nhận plasmid đƣợc trình bày hình 3.2 Hình 3.2 Kết thu nhận cắt mở vòng plasmid pET-His; giếng 1, thang DNA 1kb; giếng 2, Plasmid pET-His; giếng 3, plasmid pET-His ... chứa plasmid tái tổ hợp pET-fgf2 Những khuẩn lạc cho kết d ơng tính đƣợc tách plasmid, tiếp tục kiểm tra diện gen fgf2 phƣơng pháp PCR plasmid enzyme cắt hạn chế 3.1.5 Kiểm tra plasmid tái tổ hợp...

Ngày tải lên: 26/11/2015, 08:21

... thực luận văn, thu đƣợc kết nhƣ sau: - Đã tạo đƣợc d ng vi khuẩn E coli DH5α mang vectortái tổ hợp pET-fgf2 - Đã tạo đƣợc d ng vi khuẩn E coli BL21(DE3) mang vector pET-fgf2 có khả biểu rhFGF-2 tế ... rhFGF-2 tế bào chất - Đã tiến hành lên men thu nhận đƣợc 0,23g protein rhFGF-2 từ 1L d ch lên men - Đã tinh chế đƣợc protein rhFGF-2 từ d ch lên men sắc ký trao đổi ion sắc kí lực với độ tinh đạt ... lớn FGF-2 d ng tan tế bào chất E coli - Khảo sát tối ƣu hóa quy trình tinh chế FGF-2 sắc ký trao đổi cation sắc ký lực heparin - Tiến hành quy trình lên men tinh chế quy mô lớn - Xây d ng quy...

Ngày tải lên: 26/11/2015, 08:21