regulation of fibroblast growth factor receptor fgfr
Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... presence of NaTDC (data not shown) In the case of NcC2, no inhibition of the lipase activity was observed, as 100% of the lipase activity still remained even at a molar excess of 45 The effect of NcC2 ... N-terminal and C-terminal domain sequences of each protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the ... presence of bile salt depended not only on the presence of the classic C-terminal domain, but also on the nature of the N-terminal domain Inhibition of the PL by the chimeras In the presence of a...
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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
... ligandbinding domain of nicotinic receptors However, the fact that this protein is most closely related to the a7 subunit of the neuronal AChR (24% identity of amino acids) than each of the muscle AChR ... study of MG Results Rationale for the construction and testing of AChR ECD variants N-Terminal addition of the FLAG peptide (DYKDDDDK) or addition of the first transmembrane amino acid of the ... 10%) The presence of the FLAG tag increased expression of c1–218 by almost 20%, but did not improve expression of c1–219 The presence of the HIS tag alone reduced the expression of both constructs...
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Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot
... cell surface and the ligand-binding epitopes of both receptors Deletion of a single domain in the membrane-proximal part of gp130 leads to a shift of the receptor areas involved in ligand binding ... distance or a twist of the cytoplasmic parts of the receptors responsible for signal transduction Replacement of single membrane-proximal FNIII-domains of gp130 by corresponding domains of GCSFR leads ... assumed to compensate for the shift of the ligand-binding epitopes of the receptors The domain architecture of GCSFR is identical to that of gp130; moreover these receptors share 46% sequence homology...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
... level of the lacZ gene immediately after the addition of o-phen to minimize the effect of the growth inhibition by o-phen on the transcriptional activity of VnfA Figure shows the time course of ... environmental factors affecting AnfA activity remain unknown because of the absence of the N-terminal domain in this variant Because sensing is a principal function of regulatory proteins, the isolation of ... modulation of 0.8 mT The intensities of the spectra were normalized with native signals of Mn2+ species from E coli By contrast to the results of the EPR performed in vivo, the UV-visible spectrum of...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... 2.88 2.72 3.18 2.84 Od2 of Asp423 OH of Ser425 NH of Ala490 NE1 of Trp664 O3 O6 O7 O7 2.89 2.64 2.94 2.40 NH of Ala490 Od2 of Asp636 Od1 of Asp524a Od2 of Asp524a O7 2.63 OH of Tyr590 NAG3 ()1) NAG4 ... result of conformational changes, but of the removal of negative charge at these residues Conformation of chito-oligosaccharides bound to the active site cleft For the structural determination of ... we constructed a number of single point mutants of AD2 by site-directed mutagenesis Figure shows the sequence alignment of three family 18 chitinases The side-chains of three residues (Asp522,...
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Báo cáo khoa học: Dissecting the role of the N-terminal metal-binding domains in activating the yeast copper ATPase in vivo pptx
... membranous CPC motif of the Cd2+ATPase of Listeria monocytogenes Biochem J 369, 681–685 41 Voskoboinik I, Mar J, Strausak D & Camakaris J (2001) The regulation of catalytic activity of the Menkes copper-translocating ... which the copper-binding cysteines of M1 are changed to serines (i.e M1 cannot bind copper) (Fig 1D) Coexpression of Atx1 with M1ssM2Ccc2 restored the growth of atx1Dccc2D in the selective medium, ... Expression of Ccc2 using the pX vector Immunodetection of endogenous Ccc2 in the wild-type strain YPH499 (1) and immunodetection of Ccc2 provided by the pX– vector in the presence of Atx1 (pC–Atx1)...
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt
... ClpC consists of two AAA+ domains, the first of which contains an additional N-domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker ... tight regulation of virulence genes, and cell adhesion and invasion in the pathogen Listeria monocytogenes [24–26] It has recently been demonstrated that partial disruption of heat-shock regulation ... P Kar et al Functional characterization of M tuberculosis ClpC Fig Amino acid sequence of M tuberculosis ClpC1 The deduced amino acid sequence of ClpC1 of M tuberculosis encoded by Rv3596c is...
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Báo cáo hóa học: " Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function" docx
... The mature form of the F protein present on the surface of the virus and infected cells is believed to consist of a homotrimer consisting of three non-covalently associated units of F1-F2 This ... processing of F0 to F1 and F2 Mutation of residues 69 and 322 dramatically reduced the levels of total protein immunoprecipitated as well the levels of F0 processed to F1 and F2 None of the mutations ... site-directed mutagenesis of the HRSV F protein JL generated the computer model of the HRSV F ECD AD and RS participated in the design of the experiments, oversight of the conduct of the experiments,...
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Báo cáo y học: " Role of the C-terminal domain of the HIV-1 glycoprotein in cell-to-cell viral transmission between T lymphocytes" potx
... lack of mutant glycoprotein incorporation [19,21] In a previous study, aimed at further study- Page of 11 ing the defective phenotype of HIV-Env-Tr712, we had also analysed the infectivities of ... (
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Báo cáo y học: "Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection" ppt
... http://www.retrovirology.com/content/4/1/94 Figure of early steps of replication of SIV with length or truncated Env in rhesus monkey PBMC full Comparison Comparison of early steps of replication of SIV with full length ... ng/well of p27 antigen were used for inoculation of JC-53B cells The infectivity of particles was measured by removal of the media after days, fixation and staining of cells with X-gal The percent of ... investigate the effects of the long cytoplasmic tail of the Env glycoprotein on early steps of replication as well as assembly of SIV We further compared the replication of these viruses in dividing...
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The role of the n terminal extension domain of vamp4 in the regulation of its recycling to the TRANS GOLGI network
... THE ROLE OF THE N-TERMINAL EXTENSION DOMAIN OF VAMP4 IN THE REGULATION OF ITS RECYCLING TO THE TRANS-GOLGI NETWORK TRAN THI TON HOAI INSTITUTE OF MOLECULAR AND CELL BIOLOGY DEPARTMENT OF BIOCHEMISTRY ... structure of SNAREs 25 1.3.2 General mode of action of SNAREs 37 1.3.3 1.4 1.2.2 1.3 Vesicular transport 1.2.1 1.2 SNARE localization and the specificity of transport 41 44 VAMPs and the focus of this ... Discussion Chapter 132 Role(s) of the N-terminal extension of VAMP4 in the 137 recycling of the protein 6.1 The recycling of V4nV5-EGFP mutants 137 6.2 The recycling of VAMP4-EGFP mutants 140 vi...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... osteoclast formation via regulation of NFATc1 expression Immunol Lett 2010; 131:166-9 25 Miyake H, Hara I, Yamanaka K, Muramaki M, Gleave M, Eto H Introduction of insulin-like growth factor binding protein-2 ... dimerization of receptor tyrosine kinases [30] Since EphR is often overexpressed in cancer and involved in metastasis [31, 32], it is definitely worth further exploration on whether overexpression of RIZ1 ... from the Cancer Research Society of Canada W Sun would also like to acknowledge the College of Pharmacy and Nutrition, University of Saskatchewan for the awarding of a graduate scholarship http://www.medsci.org...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... Infection of gastric epithelial cells with H pylori was also reported to profoundly activate numerous receptor tyrosine kinases (RTKs) in a T4SS-dependent fashion, including the epidermal growth factor ... epidermal growth factor receptor (EGFR) [23,24], hepatocyte growth factor receptor c-Met [23] and Her2 ⁄ Neu [23] Studies on the downstream signalling indicated that each of these RTKs can activate ... (15) and the onset of mitogenic genes and cell proliferation (16), as well as inhibit apoptosis (17) The interplay of each of these pathways could result in substantial deregulation and oncogenic...
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Tài liệu Rethinking the Role of the State in Finance doc
... Life Professor of Finance and Professor of Economics at the Wharton School of the University of Pennsylvania Thorsten Beck Professor of Economics and Chairman of the European Banking Center, ... of people with a bank account (from user survey) Percent of firms with line of credit (all firms) Percent of firms with line of credit (small firms) Percent of market capitalization outside of ... Charles Calomiris Henry Kaufmann Professor of Financial Institutions, Graduate School of Business, Columbia University Gerard Caprio William Brough Professor of Economics and Chair, Center for...
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Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
... transcription factors [8,9] However, even when HD proteins bind their cognate DNA-binding site, they generally bind to other DNA-binding cofactors [10–12] Meis2 is a member of the TALE superfamily of HD ... alternative splicing of Meis2 or other Meis family members might affect the autoinhibitory function of the Hth domain Database searching revealed the presence of two isoforms of human Meis3 (termed ... 5¢-end of exon (Fig 7A) The region missing in Meis3.2 encodes the equivalent of amino acids 164–180 in Meis2, which form about half of hr2 (see Fig 6A) To confirm that the two isoforms of Meis3...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... reactive site loops of a1-antichymotrypsin and heparin cofactor II contain leucine instead of arginine as P1 residues, which explains why the formation of a stable inhibitory complexes of these serpins ... correlation factor of 0.36 and an R -factor of 0.46; the corresponding values of the next best solution were 0.22 and 0.50 Crystallographic refinement was carried out over several cycles consisting of model ... development of specific inhibitors of individual TTSPs may provide insight into the molecular mechanisms of carcinogenesis as well as the normal biological roles of this interesting, emerging class of...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... dependence on l-dopa as cofactor to eliminate the characteristic lag period of tyrosinases [8,20,21] Figure shows the rate of TH activity as a function of the concentration of l-dopa cofactor added to ... Journal 273 (2006) 257–270 ª 2005 FEBS Fig Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor, L-dopa (n) R3; (d) R3-0337– which only expresses ... the absence of lag period in the absence of l-dopa cofactor This indicates that the dead-end inactive complex (Fig 7B) is not formed in this particular enzyme Titration of the amount of l-dopa...
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Tài liệu Báo cáo khóa học: The PAS fold A redefinition of the PAS domain based upon structural prediction ppt
... [6] of the fern photoreceptor protein from Adiantum capillus-veneris has a ˚ resolution of 2.7 A, and the N-terminal domain of the human-Erg potassium channel (1BYW) [23] has a resolu˚ tion of ... structure of the N-terminal PAS domain of human PAS kinase [1] have also been determined Recently, two further structures of PAS-like domains have been solved; the periplasmic ligand-binding domain of ... domain of the human ether-a-go-go-related potassium channel, HERG (first 3D model of a eukaryotic PAS domain [23]), the FMN containing phototropin module of the chimeric fern Adiantum photoreceptor...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... formation of supercomplexes and the effect of the absence of intact complex I, but the results are too preliminary in this regard Heterologous expression of the ESSS-HA subunit The localization of the ... at the position of the histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex III revealed the presence of these complexes ... expression, and it is still unclear how the level of a complex of 46 subunits is determined The activities of complex II and the downstream complex III of the electron transport chain were measured...
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