NGHIÊN cứu ĐỊNH LƯỢNG ĐỒNG THỜI PARACETAMOL và IBUPROFEN TRONG CHẾ PHẨM BẰNG BIẾN đổi WAVELET LIÊN tục

WWW.DAYKEMQUYNHON.UCOZ.COM B GIO DC V O TO B Y T TRNG I HC DC H NI NG TH H LY NGHIấN CU NH LNG NG THI PARACETAMOL V IBUPROFEN TRONG CH PHM BNG BIN I WAVELET LIấN TC LUN VN THC S DC HC H NI 2013 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM B GIO DC V O TO B Y T TRNG I HC DC H NI NG TH H LY NGHIấN CU NH LNG NG THI PARACETAMOL V IBUPROFEN TRONG CH PHM BNG BIN I WAVELET LIấN TC LUN VN THC S DC HC CHUYấN NGNH: KIM NGHIM THUC- C CHT M S: 60720410 Ngi hng dn khoa hc: TS.V ng Hong H NI 2013 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM LI CM N Trc tiờn tụi xin by t lũng bit n sõu sc v chõn thnh nht ti thy TS V ng Hong ngi ó tn tỡnh ch bo, hng dn, ng viờn tụi hon thnh lun ny Tụi xin gi li cm n chõn thnh ti cỏc thy cụ v anh ch k thut viờn ca b mụn Húa phõn tớch v c cht ó giỳp tụi thc hin lun Tụi xin c cm ti ton th cỏn b, ging viờn trng i hc Dc H Ni vỡ s dỡu dt, dy bo tụi nhng nm thỏng hc ti trng Cui cựng tụi xin by t lũng bit n ti gia ỡnh, bn bố ó luụn giỳp khớch l tụi sut thi gian hc v lm lun H Ni, ngy 11 thỏng 11 nm 2013 Hc viờn ng Th H Ly WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM MC LC Trang DANH MC CC CH VIT TT DANH MC CC BNG DANH MC CC HèNH V T VN ấ CHNG 1: TNG QUAN 1.1 PHNG PHP QUANG PH O HM 1.1.1 nh lut Lambert-Beer 1.1.2 Quang ph o hm 1.1.3 Phng phỏp ph o hm t i 1.2 PHẫP BIN I WAVELET 1.2.1 Nguyờn lý Wavelet 1.2.2 nh ngha wavelet 1.2.3 Bin i wavelet liờn tc (Continuous Wavelet transform CWT) 10 1.2.4 u im ca wavelet 14 1.2.5 Mt s ng dng ni bt ca wavelet 14 1.2.5.1 Nộn tớn hiu 14 1.2.5.2 Kh nhiu 15 1.2.5.3 Mó húa ngun v mó húa kờnh 15 1.2.6 Mt s h wavelet 15 1.4 MT S C IM Lí HểA V CC PHNG PHP NH LNG PARACETAMOL V IBUROFEN 18 CHNG 2: I TNG V PHNG PHP NGHIấN CU 24 2.1 I TNG - NGUYấN LIU V THIT B 24 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 2.1.1 i tng nghiờn cu 24 2.1.2 Nguyờn liu v thit b 24 2.2 PHNG PHP NGHIấN CU 25 2.2.1 Xõy dng phng phỏp nh lng ng thi PAR v IBU bng cỏc phng phỏp quang ph UV-VIS v sc ký lng hiu nng cao 25 2.2.2 ng dng cỏc phng phỏp quang ph ó nờu v HPLC nh lng ng thi PA v IB cỏc ch phm 26 2.2.3 X lý kt qu thc nghim 26 CHNG 3: KT QU NGHIấN CU 27 3.1 PHNG PHP QUANG PH T NGOI 27 3.1.1 Chun b dung mụi 27 3.1.2 Chun b mu nghiờn cu 27 3.1.3 Xõy dng cỏc phng phỏp nh lng 28 3.1.3.1 Xỏc nh khong cng tớnh 28 3.1.3.2 Chn bc súng nh lng 29 A/ o hm bc ph hp th 29 B/ o hm bc ph t i 30 C/ Bin i wavelet liờn tc ph o hm bc 31 D/ Bin i wavelet liờn tc ph hp th 34 E/ Phộp bin i wavelet ph t i 37 F/ o hm bc ph wavelet 41 3.1.4 Kho sỏt khong tuyn tớnh 42 3.2 PHNG PHP HPLC 44 3.2.1 Chun b mu nghiờn cu 44 3.2.2.Xõy dng phng phỏp HPLC 44 3.2.3 Thm nh phng phỏp 45 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 3.2.3.1 Kho sỏt khong nng tuyn tớnh 45 3.2.3.2 Kho sỏt tớnh thớch hp ca h thng 46 3.2.3.3 ỳng v lp ca phng phỏp 47 3.3 Kt qu phộp nh lng 47 CHNG 4: BN LUN, KT LUN V KIN NGH 49 4.1 BN LUN 49 4.2 KT QU V KIN NGH . 52 TI LIU THAM KHO WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM DANH MC CC CH VIT TT ACN : Acetonitril CWT : Continuous Wavelet Transform CWT PH : Wavelet liờn tc ph o hm CWT PHB1 : Wavelet liờn tc ph o hm bc CWT PHT : Wavelet liờn tc ph hp th CWT PT : Wavelet liờn tc ph t i HB1 PHT : o hm bc ph hp th HB1 PT : o hm bc ph t i PHB1 CWT PHT : Ph o hm bc wavelet liờn tc ph hp th HPLC : High Performance Liquid Chromatography IB : Ibuprofen MeOH : Methanol PA : Paracetamol PH : Ph o hm PHT : Ph o hm t i PHB1 : Ph o hm bc PT : Ph t i STT : S th t STFT : Short Time Fourier Transform TLTK : Ti liu tham kho UV VIS : Ultraviolet Visible WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM DANH MC CC BNG STT Bng Tờn bng Trang 1.1 Mt s c im lý hoỏ ca Paracetamol v Ibuprofen 18 1.2 Cỏc phng phỏp nh lng Paracetamol 20 1.3 Cỏc phng phỏp nh lng Ibuprofen 21 1.4 Cỏc phng phỏp nh lng ng thi PA v IB 22 3.1 Kt qu kho sỏt khong tuyn tớnh ca IB v PA bng phng phỏp quang ph 42 3.2 Kt qu kho sỏt khong tuyn tớnh HPLC ca IB v PA 45 3.3 Kt qu kho sỏt tớnh thớch hp ca h thng sc ký 46 3.4 ỳng ca phng phỏp HPLC 47 3.5 Kt qu nh lng cỏc ch phm 48 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM DANH MC CC HèNH V STT Hỡnh Tờn hỡnh Trang 1.1 Ph hp th (a), ph o hm bc (b) v bc (c) (di ph nột chm cú cựng v trớ v cng nhng rng gp ụi); Ph hp th ca trans -stilbene cyclohexane (d) v ph o hm bc (e), bc (f) tng ng 1.2 Bin i Fourier 1.3 Bin i Fourier thi gian ngn 1.4 Bin i wavelet 1.5 Mụ t cỏc bin i ca tớn hiu 1.6 Súng sin v wavelet 10 1.7 Ba dng hm wavelet 10 1.8 Hm (t) ca h bin i symlets 11 1.9 Hm (t) ca bin i Mexican Hat 11 10 1.10 Ph trc (A) v sau (B) c loi nhiu bng wavelet liờn tc 12 11 1.11 Ph hp th (A) v ph c bin i wavelet (B) ca dóy dung dch Trimethoprim (nột lin) 28 mg/L v Sulphamethoxazol (nột chm) 26 mg/L Mi tờn ch bc súng nh lng 13 12 1.12 Hm (t) ca h bin i Sym vi h s n = 2, 3, 4, 15 13 1.13 Hm (t) ca bin i Haar 15 14 1.14 Hm (t) ca h bin i Daubechies vi h s n = 2, 3, 4, 16 15 1.15 Mt s h hm (t) ca cỏc cp h bin i Bior 16 16 1.16 Hm (t) ca h bin i Coif 16 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 17 1.17 Hm (t) ca h bin i Morl 17 18 1.18 Hm (t) ca h bin i Mexican Hat 17 19 3.1 Ph hp ca IB 20 mg/L, PA 32,5 mg/L, ph cng v ph hn hpIB 20 mg/L v PA 32,5 mg/L 28 20 3.2 PH bc ca dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 29 21 3.3 PHT bc ca dóy dung dch hn hp IB + PA chia IB v dóy dung dch PA chia IB 30 22 3.4 PHT bc ca dóy dung dch hn hp IB + PA chia PA v dóy dung dch IB chia PA 31 23 3.5 Wavelet liờn tc hm sym6 PH bc ca dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 32 24 3.6 Wavelet liờn tc hm haar PH bc ca dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 33 25 3.7 Wavelet liờn tc PHB1 hm mexh ca dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 34 26 3.8 Wavelet liờn tc ph hp th hm sym6 dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 35 27 3.9 Wavelet liờn tc ph hp th hm haar dóy dung dch IB (12 32 mg/L) v PA (20 40 mg/L) 36 28 3.10 Wavelet liờn tc ph hp th hm mexh dóy dung dch IB (12,0 32,0 mg/L) v PA (20,0 40,0 mg/L) 37 29 3.11 CWT PT hm sym6 ca dóy dung dch hn hp IB + PA chia IB v dóy dung dch PA chia IB 38 30 3.12 CWT PT hm sym6 ca dóy dung dch hn hp IB + PA chia PA v dóy dung dch IB chia PA 39 31 3.13 CWT PT hm haar ca dóy dung dch hn hp IBU + PA chia IB v dóy dung dch PA chia IB 40 32 3.14 CWT PT hm haar ca dóy dung dch hn hp IB + 41 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM Hỡnh 3.16 Sc ký gii hn hp IB 20 mg/L v PA 32,5 mg/L 3.2.3 Thm nh phng phỏp 3.2.3.1 Kho sỏt khong nng tuyn tớnh Khong nng tuyn tớnh ca phng phỏp c ỏnh giỏ da trờn s ph thuc ca nng IB v PA vi din tớch pic tng ng (bng 3.2) Bng 3.2 Kt qu kho sỏt khong tuyn tớnh HPLC ca IB v PA R2 Hot Bc Khong cht súng tuyn tớnh (nm) (mg/L) IB 221,0 12 32 y = 57,769x 87,609 0,9998 PA 221,0 20 40 y = 48,774x 20,917 0,9997 Phng trỡnh hi quy 45 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 3.2.3.2 Kho sỏt tớnh thớch hp ca h thng Tớnh thớch hp ca h thng sc ký c kho sỏt bng cỏch tiờm lp li ln mt dung dch hn hp chun IB v PA iu kin sc ký nh trờn Bng 3.3 Kt qu kho sỏt tớnh thớch hp ca h thng sc ký PA IB STT tR (phỳt) S (mAu x phỳt) tR (phỳt) S (mAu x phỳt) 0,858 1622,9 4,322 1121,5 0,858 1622,0 4,317 1121,6 0,859 1622,5 4,327 1122,4 0,858 1622,3 4,326 1121,6 0,858 1631,0 4,324 1122,5 0,857 1630,4 4,327 1121,9 XTB 0,858 1625,2 4,324 1121,9 RSD (%) 0,1 0,3 0,1 0,1 AFTB 0,77 0,80 Rs 15,40 Nhn xột: - lch chun tng i ca din tớch pic v thi gian lu ca c cht u nm gii hn cho phộp (< 2%) - phõn gii (Rs) gia pic lin k ca IB v PA t yờu cu ( 2) Nh vy, h thng sc ký c la chn phự hp cho phộp nh lng hn hp IB v PA 46 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 3.2.3.3 ỳng v lp ca phng phỏp lp ca phng phỏp c xỏc nh bng giỏ tr RSD ca ln nh lng cựng mt mu thuc ỳng ca phng phỏp c xỏc nh bng phn trm tỡm li sau thờm chớnh xỏc ng thi mt lng cht chun IB v PA (khong 10% v 20% so vi hm lng ghi trờn nhón) vo mu th Kt qu thc nhim cho thy phng phỏp HPLC cú lp (RSD < 2%) v ỳng (98 102%) cho c ba loi ch phm Alaxan, Dibulaxan v Febro (bng 3.4) Bng 3.4 ỳng ca phng phỏp HPLC % thờm % tỡm li (TB SD, n = 3) chun Alaxan Dibulaxan Febro IB PA IB PA IB PA 10 % 99,91,2 100,31,1 99,70,9 99,81,3 100,61,1 99,60,9 20 % 100,21,0 100,20,9 100,41,2 99,51,2 99,91,0 99,51,3 3.3 Kt qu phộp nh lng Kt qu nh lng mt s ch phm thuc bt pha tiờm hin ang lu hnh trờn th trng bng cỏc phng phỏp quang ph v HPLC c trỡnh by bng 3.5 Kt qu cho thy khụng cú s sai khỏc cú ý ngha thng kờ (p > 0,05) v ỳng (ANOVA) v lp (Bartlett) so sỏnh cỏc s liu ca cỏc phng phỏp quang ph v HPLC vi cỏc ch phm em nh lng Bng 3.5 Kt qu nh lng cỏc ch phm 47 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM Phng phỏp Dibulaxan Febro PA IB PA IB PA 99,4 0,9 99,8 0,9 99,8 1,3 99,4 1,0 100,3 1,0 99,3 1,2 HB1 99,5 0,9 100,1 1,4 100,3 1,7 99,6 1,3 99,4 0,9 99,3 1,3 HT 99,8 1,3 99,1 1,1 100,2 1,5 99,3 0,9 99,4 1,5 100,1 1,4 Sym6 100,3 1,8 99,6 1,3 100.3 1,5 99,4 1,2 100,6 0,8 99,1 1,0 Haar 99,3 1,3 99,1 1,2 100,3 0,9 99,7 1,7 100,5 1,2 99,4 0,9 Mexh 101,0 1,2 100,7 0,8 100,5 1,8 98,8 1,3 99,4 0,9 99,6 1,1 CWT Sym6 100,1 1,1 99,1 1,3 99,4 0,4 100,4 0,9 99,2 1,1 99,8 0,9 PT Haar 99,7 0,9 99,3 0,6 100,1 1,0 99,6 1,1 100,6 1,3 99,5 1,1 Sym6 100,8 1,4 100,0 1,0 99,7 1,2 101,0 1,1 99,5 1,4 100,5 0,9 Haar 99,5 0,8 99,5 1,0 100,7 1,1 100,9 1,3 100,7 0,8 99,8 0,8 Mexh 99,7 1,0 99,5 0,9 99,8 1,3 100,2 1,5 99,7 0,9 99,8 1,2 Sym6 100,5 1,1 100,5 0,8 99,8 1,5 99,3 1,4 100,6 0,8 100,0 1.1 o hm CWT PHT CWT PHB1 HB1 CWT Bng 3.5 Kt qu nh lng cỏc ch phm IB HPLC Hm lng % so vi nhón ( trung bỡnh SD) Alaxan PHT 48 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM CHNG 4: BN LUN, KT LUN V KIN NGH 4.1 BN LUN nh lng ng thi cỏc hot cht ch phm cú cha 2, thnh phn luụn l mt tr ngi vi quang ph hp th UV-Vis s an xen cỏc di ph riờng bit ca tng cht i vi hn hp IB 20 mg/L v PA 32,5 mg/L, vic nh lng IB hon ton khụng th thc hin bng phng phỏp quang ph UV truyn thng khong bc súng 200 240 nm (hỡnh 3.1) tin hnh nh lng c ng thi IB v PA hn hp, cỏc thut toỏn bin i o hm v wavelet ph hp th ó c ng dng khúa lun ny (hỡnh 3.2 3.15) Cho ti nay, phộp bin i o hm thng c ỏp dng cho cỏc nghiờn cu nh lng hn hp a thnh phn bng quang ph UV-Vis trờn th gii [16, 17, 34, 39, 40 43, 45, 48, 49] v Vit Nam [1, 8, 11 15] õy l phng phỏp cú xu hng n gin húa v mt k thut cựng vi s phỏt trin ca cỏc mỏy o quang hin i v cụng ngh tin hc Ngoi ra, phng phỏp ny cũn cú u im ni bt l tit kim thi gian, dung mụi húa cht Tuy nhiờn, phng phỏp ny nhiu khụng th phõn tỏch c cỏc di ph an xen mt cỏch hiu qua cng tớn hiu v t s tớn hiu - nhiu cú xu hng gim mnh s bc o hm tng Phộp bin i wavelet liờn tc ng dng vo nh lng ng thi cỏc hn hp a thnh phn bng quang ph ó khc phc c nhc im trờn ca phng phỏp o hm [22 32, 38, 41, 46] So vi o hm, phộp bin i wavelet cú kh nng lm tng s giao im khụng v s cc tr giỳp cho vic nh lng ng thi hn hp nhiu thnh phn c tin hnh d dng hn ỏng chỳ ý, khụng phi tin hnh lm trn ph sau phộp bin i wavelet l mt u im ni bt ca phng phỏp ny Ngoi ra, s vt tri v cng tớn hiu o ca wavelet so vi o hm cng gúp phn lm tng nhy ca phộp nh lng, c bit vi cỏc cht cú hm lng thp hn hp 49 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM PP quang ph o hm Sym6 CWT HB1 PHT HB1 PT CWT PHT CWT HB1 Mexh Haar Haar Mexh CWT PT Sym6 Sym6 Haar HB1 CWT PHT Hỡnh 4.1 S cỏc phng phỏp nh lng c tin hnh Yu t chớnh nh hng n vic nh lng bng CWT chớnh l vic la chn hm v cỏc thụng s ca hm ny CWT gm hm: sym, bior, coif, haar, db, dmey, mexh, meyr, morl, qua kho sỏt s b hm sym, haar v mexh ó c la chn nh lng ng thi hn hp IB v PA Vi mi hm, cỏc giỏ tr biờn (a) v tn s (f) ó ln lt c kho sỏt Trong cỏc giỏ tr h s t n ca hm sym, giỏ tr sym6 ó c la chn, giỏ tr biờn ca c ba hm sym6, haar, mexh u l a = 256 v ti õy cng tớn hiu ca ph CWT l ln nht Tn s f qua kho sỏt c la chn ln 50 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM lt l sym6 (f = 0,182), haar (f = 0,249), mexh (f = 0,063) Cú th nhn thy hm sym vi u im ni bt l s cỏc giao im khụng v s cỏc cc tr l nhiu nht (hỡnh 3.5, 3.8, 3.15) Trong ú, hm mexh li cú cng tớn hiu wavelet ln nht (hỡnh 3.7, 3.10) Hm haar cho s giao im khụng v s cc tr ớt hn hm sym nhng nhiu hn hm mexh, giỏ tr biờn ca hm ny ln hn hm sym v nh hn hm mexh Do vy, cú th coi hm haar l trung bỡnh ca hm mexh v sym Trong khúa lun ny, cỏc phng phỏp nh lng bng quang ph cú kt hp c o hm v wavelet ó c tin hnh theo hai hng, bin i wavelet ca cỏc ph o hm bc v ngc li (hỡnh 3.17) Vi hng th nht, tớn hiu ph o hm ó c tng lờn ỏng k sau phộp bin i wavelet liờn tc Kt qu cho thy c hm sym6, haar, mexh u cú th ỏp dng bin i ph o hm bc cho phộp nh lng c ng thi hn hp IB v PA hn hp (hỡnh 3.5, 3.6, 3.7) Vi hng th hai, tin hnh o hm bc ph bin i wavelet liờn tc lm tng s giao im khụng v s cc tr nhng gim ỏng k cng tớn hiu Do vy, sym6 l hm nht c ng dng nh lng ng thi PA v IB (hỡnh 3.15) Trong khúa lun ny, HPLC c s dng lm phng phỏp i chiu Kt qu thm nh phng phỏp HPLC theo bng 3.4 cho thy phng phỏp HPLC ỏp ng c yờu cu cho phộp nh lng ng thi IB v PA ch phm viờn nộn So sỏnh thng kờ cho thy khụng cú s sai khỏc cú ý ngha thng kờ vi tin cy 95% v chớnh xỏc ( lp v ỳng) gia cỏc phng phỏp quang ph bin i o hm, wavelet liờn tc so vi HPLC Do vy, phng phỏp quang ph c xõy dng hon ton cú th thay th HPLC nh lng ng thi IB v PA cỏc ch phm viờn nộn c kho sỏt 51 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 4.2 KT QU V KIN NGH * Da vo kt qu nghiờn cu ca ti, chỳng tụi ó rỳt mt s nhn xột nh sau: - Xõy dng c phộp nh lng ng thi PA v IB hn hp mt cỏch trc tip khụng qua quỏ trỡnh chit tỏch bng cỏc phng phỏp quang ph o hm v wavelet liờn tc vi chớnh xỏc ( lp li v ỳng) cao S khỏc bit v chớnh xỏc ca cỏc phng phỏp quang ph v HPLC khụng cú ý ngha thng kờ vi mc tin cy 95% - Cỏc phng phỏp quang ph ó nờu cú yờu cu k thut n gin, tit kim thi gian v ch s dng cỏc dung mụi, húa cht thụng thng nờn cú kh nng ng dng vo cụng tỏc kim nghim thuc - Khụng cú s sai khỏc ỏng k gia hm lng thc t ca cỏc mu thuc c kim nghim so vi hm lng ghi trờn nhón * Trờn c s nhng kt qu t c, chỳng tụi cú mt s kin ngh nh sau: - Tip tc nghiờn cu ng dng phộp bin i wavelet vo kim nghim cỏc dng bo ch a thnh phn cú kh nng hp th UV VIS - ng dng chn lc cỏc phng phỏp quang ph ó nờu vo nh lng ng thi PA v IB cỏc viờn nộn Alaxan, Dibulaxan v Febro ti cỏc c s kim nghim cú trang b mỏy o quang hin i 52 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM TI LIU THAM KHO Ting Vit inh Th Hi Bỡnh, Lờ Th Ngc Dip, Nguyn Thanh Hi v Trnh Vn Lu (2010), nh lng ng thi Loratadin v Pseudoephedrin bng phng phỏp quang ph o hm bc nht, Tp Kim nghim thuc s 2, 13 17 B mụn Húa dc (2006), Húa dc 1, Trng i hc Dc H Ni, 81 82, 85 86 B mụn Húa phõn tớch (2006), Húa phõn tớch II, Trng i hc Dc H Ni, 41 46, 125 146, 173 209 B Y t (2009), Dc in Vit Nam IV, 140 142, 208 210 B Y t, Dc in Vit Nam, xut bn ln th (2009), trang 140 142, 207 210, PL-133 PL-135 Nguyn Thnh t (2002), Nghiờn cu nh lng Paracetamol v Ibuprofen thuc a thnh phn, Khúa lun tt nghip dc s i hc khúa 1997 2002 Lờ Th Thanh Hin (2005), nh lng ng thi Paracetamol v acid mefenamic viờn nộn pamesic bng o quang ph MCA v th ho tan ca Paracetamol ch phm bng o quang ph UV VIS, Khúa lun tt nghip dc s i hc khúa 2000 2005 Trn T Hiu, Bựi Th Ho, Trnh Vn Qu v ng Trn Phng Hng (2003), nh lng ng thi Papaverin v Theophylin thuc Sedokal bng phng phỏp quang ph o hm, Tp Kim nghim thuc s 2, 19 23 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM Nguyn Phng Nhung, V ng Hong (2/2013) Tng quan ng dng quang ph o hm nh lng thuc a thnh phn, Phn I: i cng ph o hm, Tp dc hoc s 442, 10 Nguyn Phng Nhung, V ng Hong (5/2013), Tng quan ng dng quang ph o hm nh lng thuc a thnh phn, Phn II: Cỏc phng phỏp nh lng hn hp a thnh phn bng quang ph o hm, Tp dc hoc s 445, 11 Thỏi Duy Thỡn (2003), nh lng ng thi Paracetamol v Ibuprofen viờn nộn hn hp bng phng phỏp phõn tớch ton ph, Tp dc hc s 10, 26 29 12 Thỏi Nguyn Hựng Thu (2007), "nh lng trc tip dung dch nhiu thnh phn bng quang ph o hm t i", Tp Dc hc s 8, 35 38 13 Nguyn Th Mng Thựy v Nguyn c Tun (2003), nh lng Nimesulid viờn nộn bng phng phỏp quang ph o hm bc 2, Tp Dc hc s 4,16 18 14 Lờ Minh Trớ v Nguyn Mó Huy Thanh (2003), nh lng ng thi Loratadin v Pseudoephedrin sulfat ch phm viờn nộn bng phng phỏp quang ph t ngoi o hm, Tp Dc hc s 2, 28 30 15 Nguyn Tng Vy v Ngc Thanh (2003), nh lng ng thi Sulfamethoxazol v Trimethoprim viờn nộn Trasepton bng phng phỏp quang ph o hm t i, Tp dc hc s 12, 28 29 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM Ting Anh 16 A Y El-Sayed and N A El-Salem (2005), Recent Developments of Derivative Spectrophotometry and Their Analytical Applications, Analytical Sciences 21, 595 614 17 Abbas A, Davood N, Tayyebeh M, Maryam A-T, Mitra H (2009) Simultaneous spectrophotometric determination of binary mixtures of surfactants using continuous wavelet transformation, Journal of Hazardous Materials 166, 770775 18 Battu P.R, Reddy M.S (2009), RP HPLC method for simultaneous estimation of Paracetamol and Ibuprofen in tablets, Asian Journal of Research in Chemistry, Vol 2, pp 70-72 19 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WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 24 Dinc, E., Baleanu, D (2004), Application of the wavelet method for the simultaneous quantitative determination of benazepril and hydrochlorothiazide in their mixtures, Journal of AOAC International 87, 834 841 25 Dinc, E., Baleanu, D (2005), Bivariate and multivariate spectral resolution of a mixture of benazepril hydrochloride and hydrochlorothiazide in tablets by using linear regression lines, Revista De Chimie, 56, 937 942 26 Dinc, E., Ozdemir, A., Baleanu, D., Tas, K (2006), Wavelet transform with chemometric techniques for quantitative multiresolution analysis of a ternary mixture consisting of paracetamol, ascorbic acid and acetylsalicylic acid in effervescent tablets, Revista De Chimie, 57, 505 510 27 Dinc, E., Baleanu, D (2007), Continuous wavelet transform applied to the overlapping absorption signals and their ratio signals for the quantitative resolution of mixture of oxfendazole and oxyclozanide in bolus, Journal 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Simultaneous Spectral Determination of Trimethoprim and Sulphamethoxazole in tablets, Journal of Iranian Chemical Society 8, 90 99 33 Dipankar Basu, Kumar K Mahalanabis, Bimal Roy (1998), Application of least squares method in matrix form: simultaneous determination of ibuprofen and paracetamol in tablets, Journal of Pharmaceutical and Biomedical Analysis 16, 809 812 34 F S Rojas and C B Ojeda (2009), Recent development in derivative ultraviolet/visible absorption spectrophotometry: 20042008 A review, Analytica Chimica Acta 635, 22 44 35 Gabor, D (1946), Theory of communication: Journal of the Institute of Electrical Engineers 93, 429 441 36 Gerald, K (1994), A Friendly Guide to Wavelets Birkhọuser, 44 58 37 Jaideva C., Goswami Andrew K., Chan John Wiley & Sons (2011), Fundamentals of Wavelet: Theory, Algorithms, and Applications 38 Khadijeh M, Mahmoud R S, Atieh J (2010), Continuous wavelet and derivative transform applied to the overlapping spectra for the quantitative spectrophotometric multi-resolution of triamterene and hydrochlorothiazide in triamterene-H tablets, Talanta 81, 18211825 39 Liudmil A., Daniela N (2000), Resolution of overlapping UV VIS absorption bands and quantitative analysis, Chemical Society Reviews, Vol 29, 217 227 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 40 Mahmoud Reza Sohrabi, Naghmeh Kamali and Mazyar Khakpour (2011), Simultaneous spectrophotometric determination of metformin hydrochloride and glibenclamide in bimary mixtures using combined discrete and continuous wavelet transforms, The Japan Society for Analytical Chemistry, Vol.27, 1037-1041 41 Mahmoud R S, Parviz A, Elmira A E (2010), Simultaneous spectrophotometric determination of cyproterone acetate and ethinyl estradiol in tablets using continuous wavelet and derivative transform Spectrochimica Acta Part A: Molecular and Biomolecular, Spectroscopy 77, 107 111 42 M.R Khoshayand, H Abdollahi, M Shariatpanahi, A Saadatfard, A Mohammad (2008), Simultaneous spectrophometric determination of paracetamol, ibuprofen and caffin in pharmaceuticals by chemometric methods, Sience direct, Spectrochimica Acta Part A 70, 491 499 43 Ozgur M.U, Alpdogan G., Asci B (2002), A rapid spectrophotometric method to resolve ternary mixtures of Propylhenaxone, Caffeine, and Acetaminophen in tablets, Monatshefte fur Chemie, Chemical Monthly, Vol 133, 219 223 44 Prasanna Reddy Batty and MS Reddy (2009), RP HPLC Method for simultaneous estimation of paracetamol and ibuprofen in tablets, Asian Journal Research Chemistry 2(1): Jan March, 70 72 45 Rodenas V., Garcia M.S, Pedreno C.S, Albero M.I (2000), Simultaneous determination of propacetamol and paracetamol by derivative spectrophotometry, Talanta, Vol 52, 517 523 46 Sohrabi M R; Kamali N; Khakpour M (2011), Simultaneous spectrophotometric tetermination of metformin hyrochloride and gibenclamide in binary mixtures using combined discrete and continuous wavelet transforms, Analytical Sciences 27, 1037 1041 WWW.FACEBOOK.COM/DAYKEM.QUYNHON WWW.DAYKEMQUYNHON.UCOZ.COM 47 The United States Pharmacopeia 30 (2009), Vol II, pp 1266 1270, 1272, 1285 1288, 1575, 2325- 2329 48 Youssef R M and Maher H M (2008), A new hybrid double divisor ratio spectra method for the analysis of ternary mixtures, Spectrochimica Acta Part A, Vol.70, 1152 1166 49 Yousry M Issa, Sayed I.M Zayed, Ibrahim H.I Habib (2011), Simultaneous determination of ibuprofen and paracetamol using derivatives of the ratio spectra method, Arabian Journal of Chemistry 4, 259 263 50 Yao Tien Chen, Din Chang Tseng (2007), Wavelet based medical image compression with adaptive prediction, Computerized Medical Imaging and Graphics 31, 51 Yinpeng Jin, Elsa Angelini, Andrew Laine (2005), Wavelets in medical image processing: denoising, segmentation, and registration, International Topics in Biomedical Engineering, 305 358 WWW.FACEBOOK.COM/DAYKEM.QUYNHON ...WWW.DAYKEMQUYNHON.UCOZ.COM BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ Y TẾ TRƯỜNG ĐẠI HỌC DƯỢC HÀ NỘI ĐỒNG THỊ HÀ LY NGHIÊN CỨU ĐỊNH LƯỢNG ĐỒNG THỜI PARACETAMOL VÀ IBUPROFEN TRONG CHẾ PHẨM BẰNG BIẾN ĐỔI WAVELET LIÊN TỤC LUẬN VĂN THẠC... lượng đồng thời Paracetamol Ibuprofen chế phẩm biến đổi wavelet liên tục với hai mục tiêu: Xây đựng quy trình định lượng đồng thời hỗn hợp hai thành phần PA IB phép biến đổi wavelet liên tục quang... ứng 1.2 Biến đổi Fourier 1.3 Biến đổi Fourier thời gian ngắn 1.4 Biến đổi wavelet 1.5 Mô tả miền biến đổi tín hiệu 1.6 Sóng sin wavelet 10 1.7 Ba dạng hàm wavelet 10 1.8 Hàm ψ(t) họ biến đổi symlets