Nghiên cứu kháng sinh được sinh tổng hợp nhờ streptomyces 184 29

     Streptomyces 184.29  - 2015      Streptomyces 184.29       2015                                         .                         qua.  Sinh viên    1  2  2  2 Streptomyces 2 Streptomyces 3  4  4 1.2.2. Phâ 4  5  6  7  7  7 1. 8  8  8  8  10  10  10  11  12  12 -VIS) 12  12 1.7. M 13 1.7.1 9 Streptomyces sp. K01-0031 13  Streptomyces purpurascens 13  Streptomyces microflavus 14  15  15  15 2.1.2. M 15  17  18  18  18  18  19  20  20  22  23  23  23  24  25  26 LU 27  27  27 Streptomyces 184.29 28  29  30  31 3.1.6. 31  32  33  33  35  36  40  40  40  41  42  42  42  ARN Acid ribonucleic DMHC    HTKS  IR  ISP International Streptomyces Project Streptomyces  MT  MTdt  NADH Nicotinamid adenin dinucleotid SLNN  UV    4  15  16  17  17  27  28  29  30  31   32  32  33  34  36  36  37  37  38  39  39 0669g 39  40 1        Florey),      Streptomyces   .      Streptomyces 184.29  t  1. C               sinh. 2.  3.  2   1.1.  1.1.1.  et  (+)      (amylase, cellulase, glucoisomerase, protease ) [5],[15]. 1.1.2. Streptomyces 1.1.2.1.   -1, -   [5],[12],[15].    [5].   b                     [16].  ti kh-1, [...]... zac Streptomyces 184. 29 28oC, sau 6- -3o 18 2.3.2 - si o 7 -108 / - -50o Petri / - Petri o 05 ml o Petri - 0,02 mm : 19 ̅=∑ ̅ ∑ s=√ , ̅ ; Di i; s ; n 2.3.3 - HTKS - Streptomyces 184. 29 Petri zig zac MT1, MT2, MT5, MT6, MT7, 28oC – 29o HTKS 2.3.4 2.3.4.1 - Streptomyces 184. 29 -1 -6 -6 -5 Petri o C– 29oC 20 zig zac o C – 29o Petri 2.3.4.2 - Streptomyces 184. 29 ml ng 10-1 -6 -6 - Petri o C – 29o -1... 1.7 1.7.1 Streptomyces sp K01-0031 9 Streptomyces - Vân sman Trong qu antimycin A3a, A3b, A4, A7 9 Caenorhabditis elegans Artemia salina 1.7.2 Streptomyces purpurascens Anthracyclin Streptomyces 7,8,9,10-tetrahydrotetracen-5,12-quinon Streptomyces n Streptomyces purpurascens α2- 13 2-deoxy-L[18] 1.7.3 Actinomycin X2 Streptomyces microflavus Streptomyces Streptomyces Streptomyces microflavus Streptomyces. .. S aureus MT7 s B subtilis MT6 s B pumilus MT5 s B cereus MT2 0,37 1,47 1,30 1,23 0,46 ̅ (mm) 17,81 18,32 19,4 18,27 18,37 27 s h n 0,56 0,79 0,79 0,21 0,64 t Streptomyces 184. 29 -) theo B cereus 3.1.2 E coli Streptomyces 184. 29 Streptomyces 184. 29 d HTKS 3.2 1: n 3.2 t qu th ho t t nh h n s nh sau s n B cereus n u nh n E coli ̅ (mm) s ̅ (mm) s SLNN.1 20,77 0,83 21,07 0,57 SLNN.2 22,39 1,20 21,12 0,51...…) - ) [5],[13] 1.1.2.2 Streptomyces Streptomyces -30o th 6,8-7,5 S Streptomyces [5],[13] 1.1.3 Streptomyces … Streptomyces Streptomyces tli [5],[20] 3 (International Streptomyces 1.2 ng sinh 1.2.1 … [15] 1.2.2 … [1],[2],[4] n 1.1 Ph n o h n s nh β – lactam Penicillin, cefoperazon… Phenicol Chloramphenicol,... sulfamethoxazol 1.2.3 ] Streptomycin: Streptomyces griseus, - streptomy 2],[8] Neomycin: Streptomyces fradiae - olym [2],[8] Gentamicin: Micromonospora purpurera, - Staphylococcus aureus ethicillin, Pseudomonas aeruginosa [2],[8] Erythromycin: Saccharopolyspora erythraea, [2] Chloramphenicol: Streptomyces venezuelae, -) , chlorampheni nuôi, th [2] 5 Tetracyclin: Streptomyces aureofaciens Streptomyces viridofaciens... - Streptomyces 184. 29 ml ng 10-1 -6 -6 - Petri o C – 29o -1 - Petri  = 254 nm -5 - 1 -5 , 10-4 Petri 28oC – 29oC, Nm x 10-6+k x 100% N0 Nm N0 10-k 21 u nhiên HTKS = D mi x 100% D0 D mi D0 - HTKS 2.3.5 - 2.3.5.1 - HTKS HTKS 2.3.5.2 -7 NaOH 0, HCl 0,1N HTKS HTKS 22 2.3.6 - Streptomyces 184. 29 o / C ± 0, ml :V o C ± 0, / HTKS HTKS - HTKS HTKS 2.3.7 - - - HTKS - HTKS 2.3.8 pha dung môi -0,09 atm, 60o... ng cách t v ch xu n tâm c a v t Ddm: Kho ng cách t v ch xu n v ch dung u 2.3.9.2 Dung môi: P -0, 254 o -8 g / o methanol, C cho bay / - ml - c f 2.3.10 -60o 25 2.3.11   26 3 3.1 3.1.1 Streptomyces 184. 29 HTKS 3.1: n 3.1 t qu a h n tr n n n t MT1 S typhi 19,93 0,73 1,04 0,25 0,87 1,43 ̅ (mm) 20,13 21,11 17,40 20,93 19,57 0,60 0,18 1,21 0,92 1,64 ̅ (mm) 23,56 27,61 26,31 26,67 25,37 0,92 0,35... purpurascens α2- 13 2-deoxy-L[18] 1.7.3 Actinomycin X2 Streptomyces microflavus Streptomyces Streptomyces Streptomyces microflavus Streptomyces actinomycin X2, 2 14 [14] 2 G G G 2.1 2.1.1 G g g Streptomyces 184. 29 - g - n 2.1 C v s nh v t nh Bacillus cereus ATCC 9946 Bacillus pumilus ATCC 6633 S lutea Staphylococcus aureus ATCC 1128 S aureus Escherichia coli ATCC 25992 E coli Proteus mirabilis BV 108... Saccharopolyspora erythraea, [2] Chloramphenicol: Streptomyces venezuelae, -) , chlorampheni nuôi, th [2] 5 Tetracyclin: Streptomyces aureofaciens Streptomyces viridofaciens Clt rickettsia [2],[4] Vancomycin: Streptomyces orientalis, Staphylococcus aureus [1] 1.2.4 1.2.4.1 aunorubicin, d … [5],[15] 1.2.4.2 Sau khi p h (f Protozoa … - 6 n riseofulvin, trichoxetin, blasticidin S, … kasugamycin, polyoxin, validamycin,... n khác nhau các thành ph n c a c tách riêng bi t thành d i trên s nh tính ng   ] 11 1.6 1.6.1 1.6.2 -VIS) Nguyên t c: D a trên s h p th ng các b c x ánh sáng vùng UV - VIS b i các phân t c a ch t nghiên c im n t (E0, E1, E2 ) Gi a c u trúc hóa h c và quang ph h p th có m i quan h ch t ch , ch có nh ng phân t n t d b kích thích m i h tr ng chuy n t n lên tr ng thái b kích thích (phân t có n t N, .  27  Streptomyces 184. 29 28  29  30 .     Streptomyces 184. 29    .     Streptomyces 184. 29  - 2015  