Sàng lọc vi sinh vật sinh Enzym Cellulase từ ruột mối và tối ưu hóa môi trường nhằm nâng cao hoạt tính Enzym

 T  T    H Chí Minh         p cùng kinh  , các em    , cô h, t . T cô  và   em.  TP. HChí Minh   ,    luôn ng  mà em     C  luôn sát cánh   .    gi con . Kính chúc                TP.      i   IV  V  VI   VI  1  3 1.1.  QUAN  LOÀI  4 1.1.1.  4 1.1.2.   5 1.1.3.  5 1.2. CELLULOSE 5 1.2.1.  5 1.2.2.  5 1.3. ENZYM CELLULASE 8 1.3.1.  8 1.3.2.  9 1.3.3.  10 1.4. VI SINH    ENZYM CELLULASE 13 1.4.1.  13 1.4.2.  13 1.4.3.  13 1.5. TÌNH HÌNH NGHIÊN   NAY 13 1.5.1.  13 1.5.2.  14 1.6. QUY HOACH C NGHIÊM 14 1.6.1.  15 1.6.2.  15 1.6.3. 15   ii 1.6. 17 1.6.5. 19 1.6.6.  24   26 2.1.  GIAN VÀ      TÀI 27 2.2.   27  27  27  28  28 2.3.  PHÁP NGHIÊN  279 2.3.1.  29 2.3.2.  29 2.3.3.  30 2.3.4.  30 2.3.5.   31 2.3.6. Xác  31 2.3.7.  35 2.3.8.  37 N 44 3.1.   PHÂN    CÁC  VI SINH  CÓ   SINH ENZYM CELLULASE 45 3.1.1.  45 3.1.2.             cellulase. 46 3.2.   XÁC   TÍNH ENZYM CELLULASE  CÁCH   KÍNH VÒNG  PHÂN 48 3.3.   XÁC   TÍNH CMCASE 50   iii 3.4.    DANH 51 3.5.   TÔI  HOA MÔI NG CHUNG MB23A  CAC NGUÔN PHUPHÊPHÂM 53 3.5.1. 610   bào 53 3.5.2.Bacillus MB23a 54 3.5.3. 56 3.5.4.               Burman 60 3.5.5. 64 3.5.6.  Behnken 67  73 4.1.   74 4.2.   75  76 P 81   iv  ANOVA One  way analysis of variance o C Celsius degree CFU Colony forming unit CMC Cacboxylmethyl cellulose CMCase Cacboxylmethyl cellulase mL Milli liter µL Microliter NA Nutrient Agar NB Nutrient Broth nm Nanomet Nxb  PDA Potato D-glucose Agar pH puisauce de Hydrogen sp. Species   v   18 dung  33  34  45 g sinh enzym cellulase 46  48  50   52   610  53 Bacillus MB23a 54  56   57     59  Burman 60   61  655  Behnken 67   Behnken 688   vi   4    6  7 gian 8 trong không gian 8 enzym cellulase theo Erikson 10  16 Hình 1.8 18 Hình 1.9  22  Behnken 23  25   45  47 Hình 3.3  47 Hình 3.4  49 Hình 3.5  722   vii    49   50  51  610  53 Bacillus  55                55   57   58   59  63  71  71   1      9     - 95%. -- 2.500.000     hoàn toàn.  và , 2010).    e -   cellulose.             cellulose  (Ngu                   trong                                 Cellulomonas flavigena, Bacillus megaterium, Bacillus alcalophilus, Clostridium sp., Bacillus amyloliquefaciens  phân       -     2     Cellulomanas, Enterobacter và Citrobacter (Subodh   , 2012), Chryseobacterium kwangyangense, Acinetobacter, B. cereus (Ramin    vi s     enzym cellulase     -     -    cellulase  -   sinh enzym cellulase -  . -   [...]... nhi enzym Enzym Cx: c thành cellobiose - Edocellulase hay e -1,4 - Exocellulase hay e n thành disaccarit o CBH amin Enz amin o g tác nh 4,4 -90 Anh, 2007 và , 2010) 1.3.2 cellulase là - 9 thành glucose và , 2010) Hình 1.6 và , 2010) 1.3.3 , 2010) 10 nhà khoa , 2010) ellulose n tham gia vào không nhai l 2010) gia súc 11 06) ; , 2006) Trong 12 1.4 1.4.1 n còn khiêm Cellulosemonas persica sp Nov và Cellulomonas... và , 2000); vi Clostridium thermocellum, Clostridium cellulovorans (Murashima, 2002), Clostridium celluloliticus (Parsiegla, 1998) 1.4.2 cellulase n Actinomyces verne, Thermonospora curvata và Streptomyces sp và Micromonospora sp (Pasti và , 1985) 1.4.3 Các enzym Aspergillus, Alternaria tenuis, Celluvirio gilvus, Clasdosporium, Penicillium, Fusarium, Myrotherticum, Trichoderma 1.5 1.5.1 13 Subodh và. .. Schaefer và (1996), Bacillus, Paenibacillus, Streptomyces Actinobacteria Pseudomonas, Acinetobacter và Ochrobactrum 1.2 CELLULOSE 1.2.1 là 9 95% , 2010) 1.2.2 5 D glucosep 1,4 glucoside o Hình 1.2 , 2010) 1,4 r hydrogen 1,4 nm Các - 40 nm, dài 100 - 6 40000 nm - - dài phân so v quan Hình 1.3 ( Hòa, 2012) 7 Hình 1.4 Anh, 2007) 1.3 ENZYM CELLULASE 1.3.1 nh thành glucose Hình 1.5 2007) 8 Trong thiên nhi enzym. .. 1.4.3 Các enzym Aspergillus, Alternaria tenuis, Celluvirio gilvus, Clasdosporium, Penicillium, Fusarium, Myrotherticum, Trichoderma 1.5 1.5.1 13 Subodh và ( 2012) Cellulomanas, Enterobacter và Citrobacter Ramin và curvignathus (2009 Coptotermes Chryseobacterium kwangyangense, Acinetobacter, Bacillus cereus 1.5.2 (2003), (2005) 1.6 , 2011) 14 1.6.1 , 2011) 1.6.2 , 2011) - - 2009) 1.6.3 15 t Hình 1.7 ... Experiment 19 value) , 2011) 1.6.5.2 tên là l tiêu, xu , 2011) c j x 20 , , t t l 1.6.5.3 , 2011) Y: h 0 21 i i ii i ij: i và Xj Central Composite Behnken (Box và Behnke , 2011) : 2k , 2011) Hình 1.9 , 2010) th là Behnken 22 , 2011) , 2009) N = 2k + 2k + n0 Hình 1.10 Behnken (Box và Behnken, 1960) So v i thi t k CCD, thi t k Box Behnken có nh m: S l n thí nghi m cho m i l n l p ít m thí nghi cho m i... 27 - Singapore - ® - Anh - Máy Vortex Velp - Model: ZX3 Italia 300VF, STURDY Máy - mLus CK 30 - Lò vi sóng Sharp - - Cân phân tích 2.2.4 - ng NA b sung 1 % CMC - ng Gause 1, b sung 1 % CMC - ng Potato D-glucose agar (PDA) b sung 1 % CMC - B thu c nhu m Gram g m: Crystal violet, Lugol, Safranin, c n 96o Hóa ch t: Congo red, d u soi, KOH, KNaC4H4O6.4H2O, NaCl, Liên 28 ... cho m i bi t ra ngoài kho ng gi a 2 m t tr i c a Box tl p Behnken so v i CCD Do m thí nghi m d c tr c nên nhi u khi không th th c hi n 23 c thí nghi kh o sát (Nguy t ra ngoài vùng gi i h n ho ng c ng và c ng s , 2011) 1.6.6 Minit , 2011) dán Ngoài ra Minitab 24 Hình 1.11 25 PHÁP 26 2 -T 05/2014 - P H Chí Minh Bình , T - Bình Schotle - Gi y th m - Bercher - Gi y b c - B micropipet (50 - Kéo - K pg p .  5 1.3. ENZYM CELLULASE 8 1.3.1.  8 1.3.2.  9 1.3.3.  10 1.4. VI SINH    ENZYM CELLULASE 13 1.4.1 N 44 3.1.   PHÂN    CÁC  VI SINH  CÓ   SINH ENZYM CELLULASE 45 3.1.1.  45.      cellulase. 46 3.2.   XÁC   TÍNH ENZYM CELLULASE  CÁCH   KÍNH VÒNG  PHÂN 48 3.3.   XÁC   TÍNH CMCASE 50 