TổNG QUAN Tà LIệU ...
BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC BÁCH KHOA HÀ NỘI LUẬN VĂN THẠC SĨ KHOA HỌC NGÀNH: CÔNG NGHỆ SINH HỌC NGHIÊN CỨU TÁCH DÒNG KHÁNG THỂ GẮN ĐẶC HIỆU VỚI LISTERIA MONOCYTOGENES BẰNG KỸ THUẬT PHAGE DISPLAY NGUYỄN XUÂN HƯNG HÀ NỘI 2006 Tai ngay!!! Ban co the xoa dong chu nay!!! 17061131528451000000 BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC BÁCH KHOA HÀ NỘI LUẬN VĂN THẠC SĨ KHOA HỌC NGHIÊN CỨU TÁCH DÒNG KHÁNG THỂ GẮN ĐẶC HIỆU VỚI LISTERIA MONOCYTOGENES BẰNG KỸ THUẬT PHAGE DISPLAY NGÀNH: CÔNG NGHỆ SINH HỌC NGUYỄN XUÂN HƯNG Ngườ i hướng dẫn khoa học: PGS TS TÔ KIM ANH HÀ NỘI 2006 i MôC LôC MôC LôC i LờI CảM ƠN ii THUậT NGữ VIếT TắT iii LờI Mở ĐầU iv I TỉNG QUAN TµI LIƯU !"Listeria monocytogenes #$%"&'()*+"&'$&"&'$,"'-.*"Listeria monocytogenes 17 /0",'12,"&'3+4"5-6&738 24 II NộI DUNG Và PHƯƠNG PHáP NGHIÊN CứU 37 92,"7-.1 37 :! ;'()*+"&'$&"*+'-H+&#*+*#7/T&'#OU#A/+* 79 /A,"712* 80 WX"*+'@ 81 TàI LIệU THAM KHảO 83 Phô lôc 97 ii " LêI CảM ƠN N2V9*#/W7#7T3#X3%Y#7Z# (-&'#53W7#[&#*/E/%&/#8%#OEB#O\*#793#K]$!#N$!# NT# ^3G# _&/# `# K/-&'# ab0# O3&/# 8%# $3&/# /)*# /E&# 7c# `# d36&# LT&'# &'/6# $3&/# /)*# 8%# LT&'# &'/6# N/e*# /CG`# N2Vf&'# gh3# /)*# i+*/# ^/H0# a%# Dj3k# N$!# l4# mB0&'#aBQ&#`#K/-&'#LT&'#&'/6#NW#5%H#gj&'#8S7#`#d36&#^/H0#/)*#LT&'#&'/6# d367#D0G# >?# /V9&'# ,n&# 7S&# 7o&/# 8%# ,oB# >\7# 7T3# 72H&'# pB+#72o&/# &'/34&#*qB# 8%# /H%/%&/#(BS&#+&!# N2H&'# pB+#72o&/# (%G# (BS&# 8r&# 8s0# pB0k# 7T3# >?# &/S&# >Vt*#Oe# '3R.#>u# 8%# 7So&/#*/v#5FH#*@0#*+*#*+j#./-&'#LT&'#&'/6#NW#5%H#gj&'#8S7k#>w*#5367#(%# N/$!#dx#N/y#iz*/#aVf&'!## D/E&# ,y.# &%Y# 7T3#X3&#*FG# [&# *+*# 7/{Yk#*T# 7/Bj*# d36&#LT&'# &'/6#$3&/# /)*# 8%#LT&'# &'/6# N/e*# /CG# >?# '3R.# >u# 8%# ,hY# 5FH# 7T3# 72H&'# 7/f3# '30&# /)*# 7S.k#&'/34&#*qB!# i4&#*h&/# >b# 7T3#X3&#*FG# [&# '30# >o&/# 7T3#>?# 7hH# G)3# >3|B# A36&# 8%#>j&'# 834T3#/H%/%&/#(BSr&}# iii THUËT NG÷ VIÕT T¾T G,," /Y"'-.1" Lbk# 2Q7# &/3|B#&'/34&# *qB#&ỗ# (e*# */W# 7hH# *+*#Xé7# &'/36G# &/JG# /+7# /36&# &/0&/# 83# O3&/#8S7#'EY#56&/#72H&'#7/e*#./CG#&/JG#7U3#7/3IB#/b0#Gỗ3#&'BY#/h3#*/H#&'Vf3# 734B#,ù&'!## L monocytogenes#(%#Gj7# 72H&'#&/ữ&'#7/@#./hG#'EY#56&/#&'/34G#72)&'# */H# &'Vf3# 8%# >j&'# 8S7!# d3# A/BC&# &%Y# >Vt*# pB0&# 7EG# >w*# 5367# 5ë3# A/F# &r&'# /+7# 723I&#ë# &/367# >j# 7/Q.# 8%#7û# (6# 'EY# */W7# A/3# &/3ÔG# (4&# 793# :%!# MQ7# &/3|B# /V[&'# /+.# /+7# /36&# &/0&/# L monocytogenes# >?# >Vt*# /+7# 723I&k#72H&'# >b# *+*# /V[&'# /+.# G3Ô&# ,y*/k# >w*#5367#(%#O\*#Aý# G3Ô&# ,y*/# */H# 7/QY# 73|G# &r&'# q&'#,ô&'# 7H# (9ë3# 7z&/# >[&# '3F&k# >j# &/hYk# >j#*/z&/# X+*# *0H# 8%# A/F# &r&'#+.# ,ô&'#7h3#7/e*#>y0#*@0#&b!#L/o0#A/b0#7/%&/#*T&'#*@0#Aü#7/BS7#&%Y#&JG#ë#A/+&'# 7/I#'\&#>w*#/36B#893#L monocytogenes!## # gI#7hH#A/+&'#7/I#*b#&/3|B#./V[&'#./+.k#7BY#&/34&#./0'1#,3O.(0Y#(%#Gj7# 72H&'#&/÷&'#A ü#7/BS7#/36B#pBF#>I#*/)&#()*#*+*#A/+&'#7/I#7+3#7ỉ#/t.#7s#7/V#836&# A/+&'# 7/I!# g|# 7%3# (BS&# 8r&# 7/h*#Oü# A/H0# /)*#“[+'-Vt*" ` MT#./Z&'#*QB#72R*#*@0#A/+&'#7/I#7/B#>Vt*!## _ " I TỉNG QUAN TµI LIÖU # "!#LISTERIA MONOCYTOGENES" ! `-F-",'-.1"%'1*+" Listeria monocytogenes ({&# >{B# 734&# >Vt*# GT# 7F#5ë3#MB220Y# 8%# *j&'#Oe# &rG#"9=6#["]!#N2V9*#>b#Gj7#OU#5+H#*+H#>?#>|#*S.#836*#./E&#(S.#>Vt*#*/@&'#*b# A/F# &r&'# (%#Listeria [=]k# >+&'# 73&#*SY# &/Q7# (%# 5+H#*+H#*@0# aB(./12O# [ ]!# NBY# &/34&k# 7+*# '3F# *@0# *+*# 5+H# *+H# &%Y# A/T&'# 'c3#*/@&'# ,H# /)# /E&# (S.# 793#D'E&# /%&'# '3U&'# */BC&# &4&# O0B# >b# *+*# */@&'# &%Y# A/T&'# >Vt*# &'/34&# *qB# 8%# OH# O+&/#893#*+*#*/@&'#A/+*#[å&'# /F&# 536&k#ñY# 50&# /6# 7/U&'# 83# A/BC&# pBU*# 7W# >?# 7s#*/U3# 74&#*/3#“Listerella” &4&#K3231#>|#XBQ7#74&#*/3#(%#Listeria &rG#"9U3#Xq&'k# A/T&'# 7hH# 5%H# 7ck# ,h&'# pB1k# ]20G# ,V[&'k# •5F&'#")#[I# 83&/# ,0&/# &/%# 83# O3&/# 8S7# gq*k# a!# K!# M!# $11(3'12)# &rG# "98 !# ]{&# >EYk# *+*# /V[&'# /+.# O3&/# /)*# /E&# 7c# '3R.# >+&/# '3+# 7z&/# >0# ,h&'# *@0# */3# Listeria# */z&/# X+*# /[&k# */H# 7/QY# &b# 'åG# 6# (H%3:# L monocytogenes, L ivanovii, L innocua, L welshimeri, L seeligeri, 8%# L grayi [8] K/E&# 7z*/#./+7#O3&/# (H%3# ,e0# 724&# *+*# '1&# G?# /b0#"6$# 2MD_k# = $# 2MD_# *x&'# &/V# prs, ldh, vclA, vclB# 8%# iap# */v# 20# 2J&'# *b# 7/I# */30# P# 72H&'# OU# 6# (H%3# 7/Bj*# */3# Listeria 7/%&/# /03# &/+&/:# L monocytogenes# 8%#L innocua &JG# 72H&'#*ï&'# Gj7# &/bG# 72H&'# A/3# &/bG#7/q#/03#'åG#L welshimerik#L ivanoviik#8%#L seeligeri •/o&/#") [9]!## # gN*'" d# LEY# /+7# O3&/# (H%3# *@0# */3# Listeria ,e0# 724&# *+*# '1&# "6$# 2MD_k# = $# 2MD_k# iapk# prsk# vclB 8% ldh!# L/@&'# A/T&'# 56&/:# G%B# 'EY# X0&/k# */@&'# 'EY# 56&/:# G%B# >Z#[N/1H#9]!# " ;'a*"B>" Lb# 7/I# /E&# (S.# L monocytogenes# 7s# &/3|B# &'Bå&# A/+*# &/0B# 50H#'åG# >Q7k# 7/q*# r&# '30#OR*k# &V9*k# &V9*# 7/F3k# 7/e*# /CG# 8%# /E&#[":`"=]!# N/q*# r&# '30# OR*# @# Aỵ# A/z# *b# A/F# &r&'# (%# &'Bå&# */z&/# 'EY# (EY# &/3ÔG# L monocytogenes# 72H&'#*/r&# &BT3# '30#OR*# *x&'# &/V# &'Bå&#'U*#5ï&'# /+7# (EY# &/3ÔG# 83#O3&/# 8S7# 'EY#56&/#&%Y#72H&'#*/Bỗ3#7/q*#r&#[" ]!# # !h WJ%",e*'"%)"Bc*"%U3"Listeria monocytogenes" !h! 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