Nghiên ứu táh dòng kháng thể gắn đặ hiệu với listeria monoytogenes bằng kỹ thuật phage display

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p t in giố g int n lin ủro e n er a c a L mono to n cy ge es, đóng v i ò t ng qu t ì a tr ro á r nh xâm nhiễm v nh n là â n t ng nê ro o ủ sã c avi i hn vật n y [ ] à 43

!"#$% & 5 nhóm pr tein o bề m tặ chính của L monocytogenes: pr tein o gắn vớithành

tế bà thông qua tín hiệu sắp ếp o x LPXT otG; pr ein có đuôi kỵ nZớc (pr tei o n

ne với thành tế bà thông qua c c tZơng t c cộng hóa trị); GW pr tein; o o á á o

pr tein 60 và lip pr teino P o o Phần số ch biết ố lZợng gen mã h a ch từn o s ó o g

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11 p t in ủ ro e c a L mono cy tog n e es ầ C c a có đ u hứ mi n kỵ nZớề c a, s à u đó l

c c aá xit min tía ch i đ n dZơngệ Ph n đuôi” n y giúp p t in gầ “ à ro e ắn với b m t t ề ặ ế

bào vi khu n 44 A tA một p t in b m t ủẩ [ ] c , ro e ề ặ c a L mono tog n cy e es với v i t a rò

pB & 7 )&'0 # 2 #72H #&' A/ & &' ,3 j&' 7/F# r # #> # 1 0*H# 7 #* 0# #3& @ 83 A/B &# << ( Gj7C [ ]# %# #.2H 1 &#7 3

&/V 8SY ] & ># !# { # EY# ?> # X+ # & #* >y / >Vt*# 2H 1 # $7 3& 8._ @0## * L mono cy tog n e es

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*{&#> 83 A/BI# # C&#7/ 7 A/Z3 7/H+ # # I#7/e #5 H# @ #> 3 7/e* % * 0 h# * %#5 H#[<P !#]

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7 &'#2H L inno ua c # P !#[ ]

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7+*#893#0X37#(3 7 3 / 3H 1 * H *#•lN_ ! &( ( Gj 7 3& 5 G 7 7V[&') "I i# %# 7# 2H 1 # |# w # #>å&' 893#/# )#3&7 & ( & @12 0 3 # * 0# L mono tog n cy e es# 5ë3 &b b > &# 73, 7z& /3 B 8 l# # * # Hh 1 1# # 6 # %# MMk#

&/V&' A/T&' b# # * # GH73 lK N]# 8f# X %#8ï # Aþ#&' &V9* XBY &# 4 G &'# ;# % [ ]! = "# # 0X 7#3

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>j&' 8S7 b 8R# <# #* # [ 8]#7/T&' pB /# 0# E&# Hh&#]W#> •]W G ,B(1 !# H ) NV[&' 7+* & Y## # # %A/ Y B# +# W [ 6]# w*#,ï#G &/3 B| / &# E > &# Hh #]W#Ọ#'3R.#]W# 7 3&#' &2H 1 \ * w# / 7 /[&##

893 5 G 7 7 5# |# w # W# %H# <[ 9]!#K/ &E 7z*/ 7 o&/# # 2 # #7e 5j# ' &#1 L mono tog n cy e es#>? X+*##

>y&/ 7/ G ; 7 3& A# 4 # # 2H 1 # /+* *# /q0 / & ># E # Hh&#]W!#I&( ( 7 3& BY &/ 7 7 Bji# %# 2H 1 #, # Q # / *#

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pB 7 o&/#./ & /@Y 7/ &/ 7 5+# 2 E # # % # W# %H# #83 A/B &# P" !#C [ ]

~e0 7 & e /3 & ,3 & @ 3, 7z& 3# 24 #O # 6 # 6 #* 0# 1 7 1# #/ 6B# %#8 Gj7#G | #D3 & lKL K : >/ 6 k# ?#./ 7 /3 &#>Vt 5 7 o&/ e '3U&'+ # 6 *# 0# 2 #7 # #K6 # +*#72H #: A/ &' 5j#' &# @1 * 0#L mono tog n cy e es!#

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li o p protein

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> B > q&' G3 & ,y / 5 G 3&/ 8 G3 & ,y / 7/B >Vt{ # + # # Ô # * # C #O # %# Ô # * # # *#ë#>j&' 8S7 b 8R# P # #* # [ ]!#l3.H H2 1 # \ #7 3& ' & 893 5 G 7 7 5# |# w # W# %H#7/T&' B > B#p 0# { #Dk Hh ##/ 7 /b Gj7 U ( 30# #O # Hh #7W#5%H#•50H#'åG#5 /h* #*{ #B >[& &/# E&k# h #> 3 7/e 5*# %Hk# h* #*{ # 25 / B 7 B&' 7z&/#8# % * *# + # W#75%H#i)#& &4 2Q# 7#pB & 7 )&'0 # 2 #72H&' O3&/ 5 &# # 6 / /)# *!#68 1 •* W#' &# /3 G#=kP% æ&'#OU##7' &# @1 * 0# L mono tog n cy e es)# ?#G /b (3.0# H H.2 1 k#7 3& &/3|B#&/ 7# 7 &' UQ 2H # O #* *+ #83#A/B & ] G ,V[&' ' Y 5 &/# !#C # 20 # # E # 6 [ 6] L #/v Gj7# O # #U z7 (3 7 3&H 2H 1 # * 0# L @

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D/3 G#Ơ L mono cy tog n e es#ë#&'Vf3 ' Y 5 &/## E # 6 li t io is s er s !#Mw*#,ï#&'BY *[##

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O9G 5 &' A/ &'# J # + # 3&/# PO [ 8]!#L+* 2#7 Vf&' /# t.# \*#li t io isG s er s # { # 4 #* 0#> B 73 & @ &'Vf3#

>Vt 5*# +H#* H# r #"9=9#ë#+ & G g &#0 Mh* [ 9]!# 2H/# P N &'# #P 7/S.#Aû#O0B >b# k#li t io is s er s #

XFY# 2 #0 A/T&' 7/Vf '# & #XBY &#4 [6 ]: !#NBY &/3 & 7s BU3 &/ &' & G# 4 # # * # ÷ # r # "9;:k# L mono cy tog n e es &ỉ3 (# & (4 # #% Gj7 O3&/ 8S7 ' Y 5# # # E # 6& &'B /3/# Y# IG#pB0 >Vf&' 7/e*## #./ G# < 893C [ ]# #G #O #8ơ#5ï&' / 7 5 &/ , 73 Bj7 U # + # 6 # H# 4 #,ï&' 7/e / G / 53 &# *# C #* W# W #O\&#

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7 Gj7F# #* *+ # + # + # 1H#O #>å ë/ A/ 3 pB 7 7/ [ # #/o&/#P!#

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&/3 G#Ơ L mono cy tog n e es#ë#GS7 >j# #*0H Hw*# ##/ 5y &/3 G 8Ơ # %H# 6# { #/ 7/ & A3&/ 7 B&'## 2V[&' 5k# 0H#'åG &/3 G# Ơ #72ï&'#G+ k# 4 # %B 83 G G &' &# ?Hk# %#8 &/3 G 8Ơ # %H# #*7c B&' @#* 0#./ơ# &÷#G &'0 #7/ 30 ' Y# E OCY 7/ 3# # 0 /Hw* 7/ 3 / 7# # 0 # * W & &# P# H [ 8]! L/B ú# ## # A @ 5 &/6 #*@0#

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&' Y > & 8 3 7B &# " % # W # % # { [ ]!#

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