... region,substitution of Nef with the doxycyclline-dependent transactivator rtTA,mutational inactivaion of Tat and TAR and deletion of the RT and IN reading frames. HeLa cells were transduced and screened ... Molecular Genetics, University of Toronto,Toronto, Ontario, Canada.Authors’ contributionsRW was responsible for performing all experiments and analyses hereinwhich include examining the effect of ... function alone, alterations in the relative activities of the individual CLKs present, or pos-sibly modulation of another host factor. Altering relativeactivities among the individual CLKs by overexpressionor...