... respectively, in other serralysins, ser-ralysin numbering.) Because these positions areinvolved mainly in formation of the S1¢ and S2 ¢ sites[20,25], in PrtA the differences may cause an increase in ... (1995) Themetzincins – topological and sequential relationsbetween the astacins, adamalysins, serralysins, andmatrixins (collagenases) define a superfamily of zinc-peptidases. Protein Sci 4, 823–840.20 ... effect of several inhibitors on PrtA activity. ND, notdetermined. For the pretreatment of PrtA with the inhibitors,0.4 nM enzyme was incubated for 20 min in the presence of 1.0 mM inhibitor....