Tài liệu Báo cáo khoa học: Switching of the homooligomeric ATP-binding cassette transport complex MDL1 from post-translational mitochondrial import to endoplasmic reticulum insertion pptx

... The Authors Journal compilation ª 2007 FEBS Switching of the homooligomeric ATP-binding cassette transport complex MDL1 from post-translational mitochondrial import to endoplasmic reticulum insertion Simone ... in the cytoplasm. In conclusion, the NBDs of mitochondrial MDL1 are located in the matrix, whereas the NBDs of the leaderless MDL1 targeted to the ER membraneface the cytosol. MDL1 forms homooligomeric ... proteins [14]. The mitochondrial targeting signal (MTS) of pro-teins directed to the IMM is recognized by receptors of the translocase of the outer mitochondrial mem-brane (TOM complex) . The classical...

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... N-terminaldomain relative to the C-terminal domain could not bedeﬁned owing to the absence of inter-domain NOEs. To conﬁrm the molecular organization of the tandemarrangement, expression vectors were constructed ... of SAILFig. 3. Secondary structure of At3g16450.1. (A) Ribbon representa-tion of the NMR structure of At3g16450.1. These ﬁgures were pre-pared usingMOLMOL [25]. Due to the lack of NOEs, the ... arising from inter-nal dynamics of these residues on the intermediate timescale for chemical shifts. As a result, these are the leastwell-deﬁned regions of the N-terminal domain. The C-terminal...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... as the ATP-unbound and Ca2+-boundE1Ca2+form. In the histogram (Fig. 2A) of the dis-tribution of the height of the projection of the embed-ded molecule above the ﬂat membrane surface, the average ... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy-ses of SERCA were performed with the data obtained in the pres-ence of ... 5.4 ± 0.7 nmand 7.2 ± 1.0 nm (Fig. 2C). The mean value of the taller peak (7.2 nm) corresponds well to the height of the cytoplasmic domain of SERCA in the E2 state[16]. Although we used puriﬁed...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... by T-pilus assembly. The assem-bled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu. ... into target cells under speciﬁc conditions in vivo.Second, cagPAI-independent signalling events mightstimulate the transcytosis of integrin molecules from the basal to the apical side of the ... reported to induce the disrup-tion of cell–cell junctions, a loss of cell polarity and the induction of pro-inﬂammatory and mitogenicresponses (Fig. 3C). The ﬁrst detected interaction part-ner of...

Tài liệu Báo cáo khoa học: Upregulation of the a-secretase ADAM10 – risk or reason for hope? docx

... Acetylcholine esterase inhibitors,which are already used in the symptomatic treatment of AD, enhance the transport of ADAM10 to the cellsurface and the non-amylodogenic cleavage of APP[91,92].ADAM10 ... 2).A ﬁrst point of intervention within the biosyntheticpathway of ADAM10 is provided by directly interfer-ing with the expression of the gene for ADAM10: the promoter region of the gene for ADAM10 ... substrate of ADAM10 [59]. Furthermore, the GPI-anchored neuro-nal guidance molecule ephrin A5 is cleaved byADAM10 upon binding to its receptor EphA3, leading to termination of the receptor–ligand...

Tài liệu Báo cáo khoa học: Myristoylation of the dual-speciﬁcity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

... Notably, JSP1 seemed to stimulate preferentially the phosphorylation of the p46 isoform of JNK,whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms. To conﬁrm functionalactivation ... permeabilization of the outer mitochondrial membrane. Release of mitochon-drial cytochrome c triggers assembly of a caspase-9-activating complex and subsequent activation of the downstream caspase ... acid. The goal of the present study was to demonstrate directly that JSP1was myristoylated, to apply an MS approach to iden-tify the residue in JSP1 that was modiﬁed and to ana-lyze whether...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... and post-translational modiﬁcationsHSF1 is the prototype of all HSFs and the mammaliancounterpart of the single HSF of yeasts, nematodesand fruit ﬂies [3,27–29]. Deletion experiments of the Drosophila ... how the developmental activity of HSF1 isregulated. The identity of the developmental signalthat promotes a monomer -to- trimer transition of HSF1 in the olfactory epithelium warrants furtherinvestigations. ... accordance with the fundamentalrole of HSF1 in the heat shock response, the require-ment of HSF1 and HSF4 in development of the lensand olfactory epithelium is limited to the postnatalperiod,...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... Besides the cross peaks resulting from the connectivity of the vinyl protons (at5.35 p.p.m.) to the allylic protons (at 2.05 p.p.m.) inmonounsaturated chains and to the bis-allylic protons(at ... restoration of TG storage when cells approachconﬂuence. The timing of this equilibrium is inﬂuencedby cell seeding conditions. On the other hand, whencells stop growth due to the effects of ... arrest either in the G1 phase, due to the cell checkpoint mechanism under the control of p53 protein, or in G2. After a time delaythat depends on the extent of the damage, cells mayenter mitosis,...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... analyses of Olvas–GFP in the PGC. FCS wasused to measure the movement of Olvas–GFP into the cytosol out of the nuage region of the PGC. Representative correlation curves areshown (A). The measurement ... poorly understood. Recently, it was reportedthat, in Drosophila, the function of the nuage might berelated to the protection of the genome via repression of the selﬁsh genetic elements in the female ... employed to examine slow processes of replace-ment of molecules in granules from other parts of the same granules or from the cytosol. We performed ableaching experiment in a whole part of a single...

Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... in the protein stability of our mutants. There-fore, these conclusions strengthen our hypothesis that the catalytic efﬁciencies of the recombinant enzymesused in our study were related to the ... transfer from the two-electron substrate NADPH to Fd pro-ceeds in an ordered pathway, in which the observedKmvalue results from the sum of the Kmvalues for the successive interaction of the ... displaced to allow the sub-strate to move into the correct position (named ‘in’conformation). The interaction of the phenol ring of Y308 with the isoalloxazine should be preciselyadjusted to facilitate...

Xem thêm