- 01 Thac Sl, bao ve nam 2005, vdi de tai lu$n van:
vLPng (Barringtonia acutagula L.)
Bui Phifcng Thuan*, Ngo Ba Binh Tru'd'ng Oai hpc KHTN, Dai hgc Qudc gia HS Npi MO DAU
Sy phong phu ve sd lu'O'ng va da dang v4 chung loai cua dong- thue vat Viet Nam la nguon cung d p
nhOng chkt cd hoat tinh sinh hoc quy gia, trong sd dd cd lectin. Viec dieu tra khai thac va nghien cuu tinh
chit cua lectin tren thd gidi ndi chung va Viet nam ndi rieng van dang du'OC ti§n hanh manh me va thu dup'c nhi>ng ket qua kha quan. Do cd kha nang lien k§t vdi du'dng va eac hop chat chO-a du-dng (thanh
phin cau t^o quan trpng tren bk mat td bao), lectin cd ti^m nang i^ng dung rit ldn trong sinh- y hpc [2,5,6].
Vi^c nghien cCfu lua chon lectin tuong tac dac hieu vdi cac logi vi khuan cd the giiip ich trong viec dinh danh cac vi sinh vat nay [1,4]. Do cd tac dung gay ngung ket dac hieu cac vi khuan Salmonella gay doc
thyc pham, lectin hat loc vung Barringtonia acutagula L. da dup'c chung tdi lua chon cho d4 tai nghien
cuu nay.
NGUYEN LIEU VA PHU'ONG PHAP Nguyen lieu Nguyen lieu
Miu hat ldc vung {Barringtonia acutagula L.) dup'c thu tai Ha Ngi.
Cac vi khuan diJng trong nghien cuu chu yeu do Vien Ve sinh dich te Trung uong cung cap, bao pom cae
chiing E. coli. Staphylococcus aureus: Salmonella va Shigella (duoc phan lap iiy ngudn benh pham, thyc
pham hay gia sue bi nhiem khuan). Ngoai ra cdn cd rppt so chiing vi khuan ciia eae Trung tam gidng quoc t^ do Trung tam Cdng nghe Sinh hoc (OHQG Ha noi) cung d p .
Phuong phap nghien ciru: Xac dinh hoat
dp lectin bing phan i^nggay ngung kk
hdng c l u ; nudi cay va chuan bi huyen dich vi khuin, nghien ci/u tuong tae lectin- vi khuan bing phan u'ng gay ngung k^t; tinh sach lectin hat loc vi>ng bing s i c ky tren cot trao dot ion DEAE 52 va cot Ipc gel Sephadex G-75; dinh luong protein bing ky thugt Bradford; xac dinh do sach cua che pham lectin bing dien di bi§n tinh tren gel polyacrylamide (SDS-PAGE) [4]; nghien eifu anh huong eiia cac yeu td:
nhiet do, pH, tac dung (yc che eua eae loai
dudng va glycoprotein (IgG) len hoat do ciia che pham lectin.
KET QUA, THAO LUAN Tinh s^ch lectin hat loc vi>ng Tinh s^ch lectin hat loc vi>ng
K^t qua xac dinh hoat do ngung ket hong clu ciia dich chiet thd hat loc vii-ng eho thiy lectin bieu hien hoat do cao (>1600 dvnk/mg bot hat), dn dinh trong cac loai dem cd pH g i n trung tinh hoac ki4m nhe va khdng dac hieu nhdm mau.
": ' . . • ^ - . • - -:. - ^ . =^y*i^^t:.- . =^y*i^^t:.- Ir '*»--'5'-V • '-"^, V"- : . " ^ ^ ^ : • • , r : " ' - ^ - VA ?4JH ' ' > H 3 -^-•^^M.3 ^^'/afii - • * ^ ky^^^ ' ^?'fT^is5^ -••: > ^ ^*-,Tf^Sl ••-'a u « ' £ @l^' m W-^ . i ^ n
Hinh 1. Anh dien di bi4n tinh (SDS-PAGE) cac phan doan lectin loc vung
1. Cac prctem chu4n (BSA - 65, ovalbumin - 45, glyceraldehyde-3-phospnate dehydrogerase - 36, trypsinogen- 24, trypsin