Kd't qua thu dugc cho thiy dinh LVl sau khi qua cdt lpc gel da tach thanh hai dinh protein, trong dd Pllb cd ham lugng cao nhung lai cd hoat do tuong ddi tha'p (do chiia da phin la cac protein tap). Plla cd ham lugng protein thap hon nhung lai triing vdi dinh hoat dp cao nhat, do dd dugc coi la che pham (CP) chinh ciia quy trinh tinh sach. Che pham cd hidu suit la 17 % va cd dp tinh sach tang ldn 18 lln so vdi DCT.
3.4 J.3 Kiem tra do tinh sach cua che phdm lectin lpc vitng
Ke't qua SDS-PAGE (hinh 3) cho tha'y tir rat nhieu protein d miu tho (cot 2), qua sac ky trao doi ion cdn lai bdn bang polypeptide (cpt 3), sau dd qua sic ky lpc gel chi cdn mot bang duy nhat cd khdi lugng phan tu khoang 45 kDa (cot 4), chiing to che' phdm thu dugc cudi cilng da hoan toan tinh sach.
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Hinh 3- Anh dien di bien tinh (SDS-PAGE) cac phan doan lectin lpc vimg
Chti thich: 1- Cac protein chuan (BSA- 66, ovalbumin - 45, glyceraldehyde-3-phosphate
dehydrogenase - 36, trypsinogen- 24; trypsin inhibitor - 21 va a- lactalbumin - 14,2 kDa); 2- Dich chiet thd (DCT); 3 - Dinh DEAE; 4 - Che pham (CP)
3.4.2 Tinh che lectin hat dao bien {Canavalia maritima)
Trong khao sat ban diu, chiing toi da phat hien 15 trong sd 20 loai cay hp dau dugc nghien ciiu cd hoat tinh gay ngimg ket hdng cau (chie'm mpt ti le rat cao la 75%). Lectin dugc phan bd trong cac bp phan khac nhau ciia cay, nhidu nhat la d hat (thdng thudng lectin hat dau chie'm den 10% lugng protein hoa tan hoac han), nhung cung cd thd d vd than, la hoac hoa...Vi du nhu d cay hoe bui
{Sophora tomentosa L.), dich chiet tir qua cd hoat dp lectin cao han dich chiet tu
hat. Ham lugng ciia lectin d tiitig loai cung rat khac nhau, cd the' rat cao hoac rat
tha'p. Cac loai dau cd hoat tinh lectin cao nhu cam thao day {Abrtts precatorius L.), dao bie'n {Canavalia maritima)... da dugc lua chpn nghien ciiu tiep.
Kft qua xac dinh hoat dd lectin cho thay dich chiet thd (DCT) hat dao
hiin cd kha gay ngung kft hdng clu nhdm mau A on dinh nhat trong sd cac
nhdm mau, cdn PBS pH 7,4 la ddm thich hgp nhat cho vide tach va tinh che' lectin.
3.4,2 J Sdc ky tren cpt trao ddi ion DEAE A'52
Dich chi^t thd hat dao bien da dugc str dung de tinh chd' lectin bing sic ky trdn cdt trao ddi ion DEAE 52. Ket qua dugc tdm tit trdn hinh 4 va bang 7.
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