3. 5.1 Anh hudng cua pH
3.5.3.1Tdc dung ciia cdc loai saccharide len lectin lpc vicng
Tren bang 10 la sd lieu thu dugc khi xir ly che phim lectin hat lpc vimg va dich chiet hat lpc vimg thd vdi cac saccharide d cac ndng dp khac nhau.
K6t qua thu dugc cho thiy tinh dac hidu dudng ciia lectin ldc vCmg la kha rdng. Bdn canh fucose, galactose (Gal) va disaccharide cd chiia Gal trong thanh phin la lactose diu cd kha nang lic chd' boat dp lectin (d dang che phim hay trong dich chifit thd) d ndng do rit tha'p la 3,15 mM. IgG (cd chiia Gal trong thanh phin oligosaccharide) cung cd tac dung len lectin d ndng dd 31 |J.g/ml (vdi dich chieft tho) va 62 ng/ml (vdi che phim).
Nhu vay, cd thi xep lectin ldc vimg vao nhdm II theo su phan nhdm lectin dua trdn tinh dac hidu dudng [16,21].
Bang 10- Tinh dac hidu dudng cua lectin hat loc vimg
A-C^c mono- va disaccharide
Galactose (Gal) Fucose Lactose Arabinose B- Glycoprotein IgG
Nong do tdi thieu con gay ixc che (mM) Dich chie't lectin tho
3,15
3,15
3,15 100
Che pham lectin
3,15 3,15 3,15 100
Nong do toi thieu con gay lie che (pg/ml)
31 62
Ghi chu: Nhimg saccharide khong co hoat tfnh lic che o n6ng dp thii nghiem cao nha't
(200 mM): glucose, glucosamine, N-acetyl D-glucosamine, mannose, mannosamine,
fructose, L-rhamnose.
3.5.3.2 Tdc dung cua cdc loai saccharide len lectin dao Men
Kd't qua trdn bang 11 cho thay lectin dao bien cd pho dac hieu dudng kha rdng. Trong sd 12 loai dudng thir nghiem, chi cd ba loai (glucosamine, arabinose, L-rhamnose) la khdng cd tac dung len lectin (dii d ndng do rit cao 200 mMJ. Sd dudng cdn lai diu gay lie che hoat dp lectin dao bien, va phin ldn vdi miic dp ra't manh (d ndng dp 3,15 mM ddi vdi cac loai dudng va din xuat la Glc, Man, ManN, Fru va Lac; 16 ^ig/ml ddi vdi IgG). Tinh chat nay ciia lectin dao biln cho phep du doan nd cd the tuang tac vdi nhieu loai vi khuin vdi cac clu tnic dudng khac nhau trdn bl mat te bao.
Bang 11. Tinh dac hieu dudng ciia lectin hat dao bien
A-Cac mono- va disaccharide Glucose (Glc) Galactose (Gal) Mannose (Man) Mannosamine (ManN) Fructose (Fru) Fucose (Fuc) N-acetyl D- glucosamine Lactose (Lac) B- Glycoprotein IgG
Nong dp to'i thieu con gay urc che (mM) Djch chiet tho 3,15 25 3,15 3,15 3,15 25 50 3,15
Che pham lectin 3,15 25 3,15 3,15 3,15 25 50 3,15
Nong dp toi thieu con gay lie che' (pg/ml)
16 16
Ghi chu: Nhirng saccharide khOng co hoat tinh lic ch^ a n6ng do thur nghidm cao nhit
(200 mM): glucosamine, arabinose, L-rhamnose.