... effects ofa combination of key residues Further analysis of these and other mutant SODs is currently underway ACKNOWLEDGEMENTS We are indebted to G Peplow, F Yamakura and T Matsumoto for the analyses ... Hiraoka, B .Y. , Nakayama, K., Fujimura, T., Taka, H & Murayama, K (1998) Inactivation and destruction of conserved Trp159 of Fesuperoxide dismutase from Porphyromonas gingivalis by hydrogen peroxide ... S., Tamagawa, H., Iwakura, K., Tsunasawa, S & Tsunemitsu, A (1990) Characterization of superoxide dismutases purified from either anaerobically maintained or aerated Bacteroides gingivalis J Bacteriol...
... thorax and abdomen (data not shown) Discussion The precise qualitative and quantitative regulation of eukaryotic gene transcription is an extremely complex process A large body of experimental ... critical for the activation ofthe a- F1-ATPase promoter Computer analysis revealed the presence of two DNA sequence motifs in this region potentially recognized bythe GAGA factor (GAF) andthe alcohol ... complexes For this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic subunits have been often used as markers for mitochondrial biogenesis [6,31,44,45] The Drosophila...
... stage andthe overall availability of transcription factors Gly We made a comparative analysis of two tRNA1 gene copies, which belonged to the highly and poorly transcribed groups The lower stability ... downregulated or completely shut off By contrast, when there is demand for large excesses ofa particular type of tRNA, as in the PSG, and sufficient quantities of transcription factors are available, ... and 100·) ofthe unlabelled fragment (Fig 2D, right, lanes and 4), but not bythe fragment from whichthe TATATAA sequences were mutated to GATATCA, at the same concentrations (lanes and 6) These...
... fact that 'holes' in the assembly - that is, Table Comparison between initial, assisted, and theoretical 2× canine assemblies Canis familiaris - 2× assembly Initial draft Assisted Theoretical ... initial 2× draft and 97.9% in the 2× assisted assembly 2× mammalian assemblies A major application for the assisted assembly algorithm isthe 2× mammalian genomes sequenced for annotation ofthe ... References Validation: assembly proximity test This section defines what a 'valid' pair of k-mers is, for the proximity validation test We start by fixing a target assembly (for example, one ofthe 2×...
... Tschesche, H (1994) The X- ray crystal structure ofthe catalytic domain of human neutrophil collagenase inhibited bya substrate analogue reveals the essentials for catalysis and specificity EMBO J 13, ... (magenta) after the binding ofa hydroxamate anda barbiturate inhibitor, respectively [22,46] All these diagrams are in the same scale, produced using GRASP synthesized by linking the P)1 and P)2 ... chemical synthesis of inhibitor analogues We thank Dr Yuch-Cheng Jean ofthe Synchrotron Radiation Research Center (Hsinchu, Taiwan) and Dr Hideaki Moriyama ofthe SPring-8 (Hyogo, Japan) for assistance...
... 110(5):743-751 Nakao F, Ihara K, Ahmed S, Sasaki Y, Kusuhara K, Takabayashi A, Nishima S, Hara T: Lack of association between CD28/CTLA-4 gene polymorphisms and atopic asthma in the Japanese population Exp ... genotyping in the SLSJ study, integration of datasets and was primary author ofthe manuscript ML performed statistical analyses in SLSJ, SAGE, CAPPS, and BHS AHP carried out statistical analyses ... ratio matrix is calculated and visually represented The y- axis shows the odds ratio on a log2 scale, which makes the odds ratio above and below on the same visual scale The IL10 genotypes are illustrated...
... Self-efficacy towards an activity is an appraisal of actual physical ability, the additional pain anticipated in performing the task andthe individual’s belief in their ability to tolerate this extra ... inevitably negative about back pain, and in a secondary analysis ofthe data from the BEAM UK [28] study (comparing manipulation, exercise and GP care) Underwood et al [29] reported that patients ... were calculated (Table 5) The result ofthis analysis suggests that no significant correlation exits between change in pain and changes in either catastrophising or fear-avoidance beliefs This supports...
... extracellular loop (EL2) of about 24 residues, anda carboxy-terminal cytoplasmic tail (Figure 2) The size ofthe carboxy-terminal tail is more variable in length; it is typically between 21 and ... carboxy-terminal tail isthe target of various posttranslational modifications, such as serine/threonine and tyrosine phosphorylation [16] and palmitoylation [17], that can significantly alter claudin localization ... on the basis of hydropathy plots, to have four transmembrane helices with their amino- and carboxyterminal tails extending into the cytoplasm [1,8] (Figure 2) The typical claudin protein contains...
... head surgery17 In our case, the lack ofa previous history of trauma, infection and head surgery leads us to believe that the AC was due to a congenital anomaly Mirror images in MZ and AC are ... et al Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts Cerebrospinal Fluid Research 2010; 7: 6-13 20 Helland CA, Aarhus M, Knappskog ... poorly understood One explanation is that later fission of MZ makes them particularly prone to this process13 The cleavage ofa single fertilized ovum usually occurs between the 3rd and 8th days of...
... contains varying amounts of collagen, extra cellular mucin and elastic tissue [9] This tissue replaces fat as the tumour invades it Thus, carcinoma is typically characterized bythe lack of isolated ... pockets of fat within its mass andthisis demonstrated in these results bythe lack of any adipose peak in the carcinoma diffraction profile The peak positions for the adipose and fibrocystic changes ... dilate, untwist, and unfold to produce a solitary locule that then enlarges as a cyst Understandably, fibrosis is often reported by pathologists in an attempt to explain clinical palpability or...
... sympatric populations of Asclepias exaltata andA syriaca (Asclepiadaceae) Am J Bot 83: 1580–1584 Burke, J M., S E Carney, and M L Arnold 1998 Hybrid fitness in the Louisiana irises: analysis of ... bands andby quantifying longdistance pollen and seed dispersal The nonrandom distribution of M lewisii and M cardinalis suggests either that the species are isolated by intrinsic aspects of their ... Hiesey et al (1971) demonstrated physiological and life-history adaptation of M cardinalis and M lewisii to the elevations at which they normally occur These species are distinguished bya number...
... deprivationinduced PC12 cell death and DNA fragmentation Survival assays (MTT-based assay) and DNA fragmentation ELISAs were performed as described in Materials and methods, andthe data are presented ... from the calculation of kd/ka, where ka isthe association rate and kd isthe dissociation rate Relative Kd is equal to Kd of IGF-I/Kd of IGF analogue Dashes indicate data inappropriate for assessing ... tightly with overall receptor af®nity but rather with the dissociation rate or occupancy time of receptor [31,32] In the present study, the analysis of association and dissociation rates of the...
... AACEVAPD1 AACEVAPD3 AACEVAPD2 AACEVAPD5 AACEVAPD4 AACEVAPD6 Vma3p Vma11p Vma16p 100 97 80 80 80 80 50 50 32 100 94 93 93 54 51 28 AACEVAPD5 AACEVAPD2 AACEVAPD3 AACEVAPD1 Table Alignments ofthe ... preparation of recombinants in yeast expression vector In the case of AACEVAPD1, and 6, TAA is used as an Acetabularia-specific codon usage (translated as Gln) Conversion of TAA to CAA was performed by ... transformant Western blot analysis was carried out to examine the assembly of V-ATPase complex in the vacuolar-membraneenriched fraction ofthe respective transformant The antibody against the...
... by evaluation of association and dissociation phases (I) and equilibrium binding data (II) as described in Materials and methods kon (MÆs))1 Gla Gla–EGFN Gla–EGFN,C Factor X Factor Xa DEGR-factor ... the fragment has a conformation that is commensurate with membrane-binding The on-rates for Gla–EGFN and Gla–EGFNC are about a factor of five higher than for the Gla-domain This can presumably ... binding isotherms The concentration dependence of factor X binding is shown in Fig It is apparent that the adsorption is rapid and that Fig Equilibrium isotherms of factor Xandits Gla-containing...
... govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes Proc Natl Acad Sci USA 96, 1240–1245 17 Takenaka A, Kizawa K, Hata T, Sato S, Misaka E-J, Tamura C & Sasida Y ... representing the PSBD ofthe dihydrosuccinyltransferase chain ofthe 2-oxoglutarate dehydrogenase complex of E coli [12] andofthe dihydroacetyltransferase chain ofthe PDH complex of B stearothermophilus ... Assembly of pyruvate dehydrogenase complex metabolite in the synthesis of fatty acids, cholesterol, steroids in eukaryotes and N-acetyl-derived carbohydrates The structural and mechanistic...
... (Novagen) Strains and media Yeast strain Ray 3A- a (a type haploid of RAY 3A- D leu2/ leu2, his3/his3, ura3/ura3, trp1/trp1) was used to isolate mitoribosome An MRP4 disruptant was constructed by transforming ... be an easy task because the number of yeast mrps as well as that of E coli ribosomal proteins may still increase [23] andthe phylogenetic identity is not always clear due to the lack of data ... Cross-genomic analysis ofthe translational systems of various organisms J Ind Microbiol Biotechnol 27, 163–169 39 Matsushita, Y. , Kitakawa, M & Isono, K (1989) Cloning and analysis ofthe nuclear genes...
... In an item analysis, the candidate items are completed by participants from the target population and analysed statistically This analysis can suggest items that may not be appropriate for the ... and IRT analysis are initially reported by construct and then the reliability and validity of final measures are explored together A) IMPAIRMENT Classical test theory approach The mean item difficulties ... the study, the analysis andthe drafting and revision ofthe manuscript MJ participated in the conception and design ofthe study andthe drafting and revision ofthe manuscript PD and DD contributed...