orf orf3 was found to consist of 507 nucleotides and encode a protein of 168 amino acids designated lbp with a calculated molecular mass of 17 kda
Construction of bacterial artificial chromosome library for kineosphaera limosa strain lpha5t and screening of genes involved in polyhydroxyalkanoate synthesis
... detoxification of media due to removal of fatty acids H Analysis of genome sequence analysis and application of PCR technique 34 Chapter Table 2.6 Applications using the BAC library as a tool Application ... presence of Gram-negative cocci in clusters and tetrad formation in an activated sludge laboratory scale reactor operated under alternating anaerobic and aerobic periods So far, a number of GAO have ... Kineosphaera limosa strain Lpha5T was constructed in vector pBeloBAC11 Lpha5T BAC library contains 7680 BAC clones with an average insert of 23.5 kb BAC library of Kineosphaera limosa strain Lpha5T was...
- 116
- 492
- 0
The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
... is a 45 kD protein consisting of an N-terminal region of about 200 amino acid containing an activation domain, a hinge region and a C-terminal DNA binding domain of about 70 amino acids E2 proteins ... (Akiyama et al., 2003) in gastric carcinoma, MN/CA9 overexpression in human renal cell carcinoma (Cho et al., 2001) and S10 0A4 metastasis associated gene in colon cancer (Nakamura and Takenaga, ... Decitabine 5AzaC 5-aza-2'-cytidine aa amino acids ADP adenosine diphosphate ACI, ACIII Albuquerque CIN I, CIN III samples AN Albuqurque normal samples AT Albuquerque tumour samples ATCC American...
- 282
- 278
- 0
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... ATPase6 COX-I COX-II COX-III Cyt b Forward primer (5’→3’) ATTCTAACCTGAATCGGAGG CCCGGACGTCTAAACCAAACC AATTACCCCCATACTCCTTACACT CCTCGGAGCTGGTAAAAA ACTACCCCGATGCATACACCACA GCCGTACGCCTAACCGCTAACA ... CGCACGGACTACAACCACGAC Reverse primer (5’→3’) GATGCTTGCATGTGTAATCT GGGGATCAATAGAGGGGGAAATA GGGTCATGGGCTGGGTTTTACTAT GGGGGTTCGATTCCTTC GGGCAATGAATGAAGCGAACAG TCGTAAGGGGTGGTTTTTCTATG GGACAGGCCCATTTGAGTATTTTG ... ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 kDa) were expressed...
- 12
- 557
- 2
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... to anoxia as described for anoxia group once and after removal of animals from desiccator, they were allowed to stay at normal conditions and were given normal diet for days and decapitated and ... glutamic acid causing excitotoxicity leading to neuronal damage in anoxia The increased concentration of TBARS and decreased concentration of TAS supports the oxidative stress in anoxia and reperfusion ... ischemia can lead to excessive release of glutamate and its accumulation in the extracellular space, which initiates the pathway of neuronal death known as excitotoxicity (35-36) Neuronal excitation...
- 8
- 622
- 0
Tài liệu .An ARCO Book ARCO is a registered trademark of Thomson Learning, Inc., and is used herein under pptx
... reelected with as wide of a margin as any candidate in the state’s history (A) she was reelected with as wide of a margin as any candidate in the state’s history (B) she had been reelected with as wide ... wide of a margin as any candidate in the state’s history (C) having been reelected with as wide a margin as any candidate in the state’s history (D) she was reelected with as wide a margin as any ... Graduate Management Admission Test, which is a standardized exam given at various locations in the United States and Canada and around the world Throughout North America and in many international...
- 696
- 1,001
- 1
Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
... thermal denaturation of DNA: average length and composition of denatured areas Nucleic Acids Res 1, 959–978 12 Musto H, Naya H, Zavala A, Romero H, AlvarezValin F & Bernardi G (2004) Correlations ... M, Nishioka M, Kakihara H, Kitabayashi M, Inoue H, Kawakami B, Oka M & Imanaka T (1997) Characterization of DNA polymerase from Pyrococcus sp strain KOD1 and its application to PCR Appl Environ ... Yamamoto T, Fujiwara S, Takagi M & Imanaka T (1999) Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon,...
- 13
- 468
- 1
Báo cáo khoa học: "Case markers and Morphology: Addressing the crux of the fluency problem in English-Hindi SMT" pot
... translation compared to medium length and long sentences Reorder: Ú я gaandhii darshana va gaandhii raashtriiya sangrahaalaya raajaghaata men hai Semantic: Conclusion Ú я gaandhii darshana va ... va gaandhii raashtriiya sangrahaalaya raajaghaata ke paara hai Here, the use of semantic relations produces the correct meaning that the locations mentioned are across ( (ke paara)) Rajghat, and ... sabase prasiddha pikanika sthala men jalon men daudatii hai gloss: in a waterway of most popular picnic spot in waters runs Reorder: a я a º pr d e antahsthaliiya jalamaarga aalapuzaa ke sabase...
- 9
- 465
- 0
DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf
... survival in cases of endometrial carcinoma Padmanabhan et al [69] revealed that MCM was associated with tumor stage and perineural invasion in prostatic intraepithelial neoplasia and invasive adenocarcinoma ... and C) and nuclei in the intestine of an early L4 larva (A , B’, and C’) are indicated by arrows Panels show DNA staining by DAPI (A and A ), EdU staining (B and B’) and merged images (C and C’) ... The Absence of the “GATC - Binding Protein SeqA” Affects DNA Replication in Salmonella enterica Serovar Typhimurium 283 Aloui Amine, Kouass Sahbani Saloua, Mihoub Mouadh, El May Alya and Landoulsi...
- 312
- 518
- 0
Lecture 3 DNA RNA and protein synthesis great
... phosphate, a sugar, and a nitrogen base DNA is in a double strand The nitrogen bases have compliment partners Adenine-Thymine Cytosine-Guanine Just a note about RNARNA is single-stranded and acts ... single-stranded and acts as a code for protein synthesis RNA is still made of nucleotides that have a phosphate, a sugar, and a nitrogen base The sugar is different and the basepairing is also different ... letters are put together) produce messenger RNA sequence codes The messenger RNA leaves the nucleus and attaches to a ribosome in the cytoplasm Transfer RNA brings amino acids to the ribosome to...
- 17
- 671
- 2
DNA, RNA and proteins
... same order as the codons , creating a protein chain • Just like linking letters to make words, linking amino acids makes proteins Amino Acid Amino Acid Amino Acid DNA and Mutations Mutations are ... DNA and Protein Synthesis • DNA contains the genetic information to make amino acids • Amino acids combine to make proteins • These proteins determine the physical traits of an organism and ... ribosome releases the protein chain DNA and Protein Synthesis - Summary DNA and Protein Synthesis Practice making mRNA using the DNA template DNA and Protein Synthesis • Amino acids are linked together...
- 18
- 1,010
- 1
Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx
... a specific amount of water was placed in a dodecahedral box with a minimum distance between the protein and box wall of 0.8 nm, and solvated with an equilibrated configuration of organic solvent ... Acknowledgements The authors acknowledge helpful discussions with Dr ´ Antonio M Baptista and Professor Susana Barreiros, and financial support from Fundacao para a Ciencia e ¸ ˜ ˆ a Tecnologia, Portugal, through ... interactions were calculated using a twinrange method [50] with short-range and long-range cut-offs ˚ ˚ of A and 14 A, respectively The SPC water model [60] was used in aqueous and in nonaqueous...
- 13
- 433
- 0
Biomass for renewable energy, fuels and chemicals
... than 60% of the state area was devoted to cropland at the time of the study and about 52% of state area was under active cultivation The major agricultural crop and farm animal wastes as well as ... Potential of Biomass TABLE 2.6 Land and Water Areas of United States a Area classification 106 Percent Nonfederal land Forest Rangeland Other land Transition land Total 179 .41 178 .66 279.09 14.41 ... the waste type The amount of available MSW, for example, is larger than the total amounts of available agricultural wastes even though much larger quantities of agricultural wastes are generated...
- 649
- 535
- 0
Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... RNA strand of the RNA:DNA hybrid formed during synthesis of the minus () strand DNA that uses (+)-strand RNA as template [2,3] HIV-1 RNase H, similar to all the RNase Hs and together with transposases, ... substrate Therefore, as described previously [20], we evaluated the ability of K-49 and KNA-53 analogues to bind to calf thymus DNA in solution and found that they are not able to intercalate into ... Alizarines as new dual HIV-1 RT inhibitors 13 14 15 16 17 18 19 20 21 22 23 F Esposito et al A new class of antiviral agents Bis-basic ethers of anthraquinone and bisalkamine esters of anthraquinonedicarboxylic...
- 14
- 425
- 0
Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc
... TGACGGGCTATCGTAAGTTTATG CACGCACTCACTACCGATCACA GAAAAGACGGTGGGGAACTACGTGTCG CGACACGTAGTTCCCCACCGTCTTTTC GAAAAGACGGTGGCGAACTACGTGTCG CGACACGTAGTTCGCCACCGTCTTTTC TGACGAATAAGGCGTTTGGTCCTTTCC GGAAAGGACCAAACGCCTTATTCGTCA ... GGAAAGGACCAAACGCCTTATTCGTCA TGACGAATAAGGCCATTGGTCCTTTCC GGAAAGGACCAATGGCCTTATTCGTCA TGACGAATAAGGCGATTGGTCCTTTCC GGAAAGGACCAATCGCCTTATTCGTCA This study S D Majumdar, PhD thesis submitted to AIIMS, 2010 ... Kaur K, Taneja NK, Chauhan S, Challu VK, Ramanathan VD, Balasangameshwara V, Kumar P et al (2010) Co-expression of DevR and DevR(N)-Aph proteins is associated with hypoxic adaptation defect and...
- 9
- 351
- 0
Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx
... substantially Namely, the DNA preincubated with l-NAP showed a diffuse migration pattern, whereas the DNA interacting with s-NAP and m-NAP migrated in a compact form similar to that of naked DNA (lane a) , ... phosphate group s-NAP a Spontaneous aggregation in vitro Pick of absorbance at 280 nmb Simplest formulac Estimated molecular mass (Da)d Calculated molecular mass (Da)e Number of monomers Estimated ... polyamines As the amino groups of polyamines are already engaged in ionic bonds with the phosphates of NAPs, secondary amino groups are those available to establish interstrand interaction with...
- 11
- 380
- 0
Methylation - From DNA, RNA and Histones to Diseases and Treatment by Anica Dricu ppt
... Hongchuan Jin, Yanning Ma, Qi Shen and Xian Wang Chapter DNA Methylation, Stem Cells and Cancer 153 Anica Dricu, Stefana Oana Purcaru, Alice Sandra Buteica, Daniela Elise Tache, Oana Daianu, Bogdan ... (Cytosine-5)-DNA Methyltransferases: Structural and Functional Characterization A Yu Ryazanova, L A Abrosimova, T S Oretskaya and E A Kubareva Additional information is available at the end of the chapter ... Bogdan Stoleru, Amelia Mihaela Dobrescu, Tiberiu Daianu and Ligia Gabriela Tataranu Chapter DNA Methylation in the Pathogenesis of Head and Neck Cancer 185 Zvonko Magić, Gordana Supić, Mirjana Branković-Magić...
- 310
- 344
- 1
Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx
... precursor of pea SiR consisting of 685 amino acids Two candidates for a poly (A) signal, ATAAA and ATAAT, were found 12 bases and 55 bases upstream of the poly (A) start site, respectively (data not ... mature PsSiR was 91% to Nicotiana tabacum SiR, 85% to Arabidopsis thaliana SiR, 79% to Zea mays SiR, 79% to Oryza sativa SiR, 59% to Cyanidioschyzon merolae SiRA, 57% to SiRB, 67% to Anabaena sp PCC7120 ... staining pattern of a 95 -kDa major band (putative heat shock protein) is shown below (D) DNA blot analysis of the pea genome Genomic DNA of pea was digested with EcoRI and HindIII and separated...
- 16
- 328
- 0
Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
... CATGTACGTTGCTATCCAGGC B-ACT-R 21 CTCCTTAATGTCACGCACGAT ASAT-D 22 TCTTTGTGATGTGTGCATTCAA ASAT-R 20 TATTCCCGTTTCCAACGAAG ALU-D 20 ACGAGGTCAGGAGATCGAGA ALU-R 19 GATCTCGGCTCACTGCAAG S3T-D 19 AATCAACCCGAGTGCAATC ... ACCTCTTGAGACGTCGATGACGTCTCAAGAGGTTC TCTTTTT (top strand) and CTAGAAAAAGAGAA CCTCTTGAGACGTCATCGACGTCTCAAGAGGTTCT (bottom strand) Targeted segments are present in all splicing variants of PRDX5 mRNA and ... GAAAAAGAAGAAGTCGTGCTGCTTCCATAAGCAG CACGACTTCTT (bottom strand) Bacterial clones with insertions in these oligonucleotides were identified using PCR and sequenced with vector primers GCTACATTTTA...
- 11
- 463
- 0
Báo cáo khoa học: "Local constraints on sentence markers and focus in Somali" doc
... waa waa s-marker (11) waxaan waxa aan waxa I doonayaa doonayaa want lacag lacag money The key things to note about (11) are as follow: • There is no sentence marker Or at any rate, the standard ... stylistic/phonological considerations): (6) baraha baa ninka sugaa ‘baa’/‘ayaa’ and ‘waa’ are in complementary distribution: every main clause has to have a sentence marker, which is nearly always one of these ... subject, with the +nom NP that appears before the sentence marker acting as a topic waxu waa baabuurka (9) wax ka I waa baabuur ka thing the +NOM s-marker truck the Any normal NP can appear as the topic...
- 8
- 366
- 0
Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc
... K., Fujiwara, M., Nakazato, E., Tanaka, K & Takahashi, H (2000) Characterization of two plastid r factors, SigA1 and SigA2, that mainly function in matured chloroplasts in Nicotiana tabacum Gene ... the PCR primers 5¢-CACA CAAGGGGTTACAAGTTCTCCACG-3¢ and 5¢-ACCA GCCAATTGGTTCCAAAAATCTATCT-3¢ and ligation into pQE31 (Qiagen) Following transformation of M15, the recombinant proteins were purified ... putative plastid RNA polymerase o factors in Arabidopsis thaliana FEBS Lett 481, 47–52 37 Tanaka, K., Tozawa, Y., Mochizuki, N., Shinozaki, K., Nagatani, A. , Wakasa, K & Takahashi, H (1997) Characterization...
- 13
- 304
- 0
Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25