leca and lecb the unveiling of their 3 d crystal structures and the revelation of rsl rs iil and cv iil
The 11th form non-english majors’ level of satisfaction with their reading comprehension lessons at phan boi chau specialized upper secondary school, nghe an
... test papers after that were collected and marked, the scores from the pre-test and post-test were calculated to compare and find out Means (M), standard deviation (SD), Degree of Freedom (df), Statistic ... exhibiting the findings of the study as well as the pedagogical implications and suggestions for further studies as the result of the discussion of those findings CHAPTER 3: FINDINGS AND DISCUSSIONS ... background knowledge and improve the other language skills And the majority of the subjects (75%) indicated their confidence in gaining knowledge of words better Most of them supposed that they learnt...
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THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
... reading They motivate, and keep students’ interest in learning and therefore, better their understanding and improve their reading skills Reading activities are considered good if: - The students ... are fond of the topic and eager to read - The students find the reading text easier to read and understand - The students can obtain or remember something about the reading text - The students ... occurs, the students tend to be bored and inattentive, poorly on tests, get discouraged about the course and may conclude that they are not good at the subjects of the course and give up (Oxford...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... ABC digestion of tendon decorin showed a high percentage of monosulfated species, the 6-sulfated one prevailing: 8% of unsulfated disaccharide, 56 and 31 % of 6- and 4-sulfated disaccharides, ... o-phenylenediamine dihydrochloride and sulfosuccinimidobiotin were obtained from Pierce, avidin conjugated with horseradish peroxidase and a 30 -kDa heparin-binding fragment of fibronectin were purchased ... presence of chondroitin/dermatan sulfate PGs at the d and e bands in the gap zone of the fibrils formed by the quarter staggered array of type I collagen molecules, and the presence of keratan...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... recognized both the 38 kDa and the 35 kDa proteins (Fig 3A, low Ca2+ eluate), and antiserum against annexin A2 also detected the 38 kDa and the 35 kDa proteins Antiserum against annexin A5 detected ... were 38 kDa and 35 kDa proteins The latter could be one of the binding proteins detected in our previous dicalcin-overlay analysis [ 13] In control studies, we did not see the binding of these ... stained with silver Amino acid sequence analysis of 38 kDa and 35 kDa proteins During the course of this study, we realized that 35 kDa proteins contained proteolytic fragments of 38 kDa proteins:...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... Materials and methods and illustrated in Fig 1A,B The conformational changes induced by DAPI on the different templates were evaluated to exclude unexpected binding and structural effects due to the ... of trinucleotide repeats, the secondary structures of individual strands are considered to be the main cause of their expansion, and are associated with a number of human genetic diseases [28] ... catalyzed on the AAT- and GC-rich random-templates The synthesis was stalled by DAPI on the ATT-bearing template giving rise to a prevalent product of about 31 33 bases (Fig 7) indicating that, in addition...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... at °C If indicated, lL of RNase A (10 mgÆmL)1) was added together with the antibody Immunopellets were washed with ice-cold IPP145, mixed with 30 lL of · SDS loading dye and analyzed by western ... family of RNA binding proteins [31 33 ] Together with the findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs ... be part of one and the same complex; on the basis of the experimental results obtained in the present study, we cannot exclude the possibility that ePAB and FRGY2 are indeed part of different...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... [EW29Ch] = 0.5 3) and the resonance signal of the side-chain NH of Trp 33 disappeared The broadened signals sharpened at the position of the second signal at [sugar] ⁄ [EW29Ch] = 3 4 and the resonance ... side-chain amide and indole groups, of residues within or adjacent to the sugar-binding site of subdomain c, identified from the crystal structure of lactose-liganded EW29Ch In the case of other ... increasing amounts of some sugars Peak movements of main chain amide and amide proton of Asp18, and side chain amide and amide proton (marked by ‘sc’) of Trp 33 in EW29Ch during the titration of lactose...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... reduced state of INT, most likely to the INT radical The rate of transition from the oxidized state to the fully reduced state upon addition of sodium dithionite depended on the medium When the ... turbid, absolute spectra of oxidized and reduced hemin in the absence and presence of inhibitors were recorded directly (Fig 3A, trace a, control, and trace b, presence of benzylimidazole) The difference ... refers to the effect of 5-deaza FAD used at a concentration that inhibited % 80% of the NADPHoxidase activity The rate and the extent of the NADPHdependent reduction of heme b were both largely decreased...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
... levels correlated positively with the degree of joint destruction over a 2-year period [16] These data extend the aforementioned observations and link IL-16 to the disease process of RA In this ... leads to alteration in their apoptotic response, the attachment of these cells to articular cartilage and subsequently to the degradation of the cartilage matrix [11, 13] This notion has been derived ... predominantly in Th1 cells and is physically associated with CD4 [20] RASFs have been identified as major producers of IL-16 in the rheumatoid joint, and it has been demonstrated that inflammatory cytokines...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
... PPAP [32 ], the Conserved Domain Database CDD [33 ], Clusters of orthologous groups KOG [34 ] and InterProScan [35 ] was also performed The results of all searches were compiled into one table and using ... Approximately g of Cy3 and g of Cy5-labelled aRNA was mixed, fragmented, volume brought to 100 L with hybridization buffer and denatured Hybridization was then conduced 16 h at 37 ‰ with medium agitation ... weeks before and three weeks after in the 20 03, 2005 and 2006 seasons Data representing individual years (20 03, 2005 and 2006) were analyzed independently in order to obtain one set of differentially...
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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation
... to isolate and examine the intrinsic dynamics of the protein, relatively unfettered and unclouded by dynamics driving unfolding and folding events An understanding of the underlying dynamics in ... when Jacobs and Monod propounded the theory of the operon in 1961 [8] Their theory was based on their observations of induction of the lac gene The isolation and characterization of the lac repressor, ... chemical nature of a gene was and what precisely it did The answers to these questions were slowly revealed with the discovery of DNA as the genetic material [4] and the discovery of the double-helical...
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Interaction of scribble with zonula occludens and intermediate filament proteins
... loss and tumorigenesis remained uncertain However the discovery of the Scrib tumor suppressor and cell polarity complex has bridged this and allowed a clearer understanding of cell polarity and ... factors and other ECM-associated ligands engage their cognate receptors like Epidermal Growth Factor Receptor (EGFR) and integrin adhesion molecules Stimulation of these receptors leads to their ... and hDlg However, unlike the modus operandi of E6, Tax does not inactivate these two proteins by targeted degradation Instead, in HTLV-1-infected T-cells, Tax binds and inactivates hScrib and...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... [15,20– 23] Here, we describe the changes associated with the aggregation states of hRad51 and hRad52 in the presence of 121-mer ssDNA and hRad52 mediated assembly of the ssDNA–hRad51 complex The ... of only ssDNA level of larger ones, thereby corroborating the effect of ssDNA induced disaggregation of hRad51 DNA/ATP induced changes in hRad51 We tested whether DNA induced disaggregation of ... had hRad52 (10 lM) with DNA The position of asterisk indicates the formation of large complex in the presence of hRad51, hRad52 and ssDNA presence of hRad52 renders much better binding of hRad51...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... elucidation of the splicing mechanism and the identification of the key amino acid residues involved in the scission and ligation of the peptide bonds have facilitated the molecular engineering of ... containing DTT The 27 kDa GST band in lane appeared smeared due to the lack of reducing agent (i.e DTT) in the SDS-loading buffer A small amount of background bands (indicated by *) in lane which disappeared ... “proteome”. [32 ] Over the past few years, a variety of proteomic techniques have been developed, allowing many thousands of proteins to be studied based on either their relative abundance, [33 ] or their...
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Báo cáo y học: " Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)" potx
... described in the Andersen's study is ANT-independent and ANT-dependent apoptosis was documented previously [ 53] Additional difference between the apoptosis described by Andersen et al from others ... were first captured on a Leica microscope and the cell length was determined using OpenLab software Average and standard deviation of cell length were calculated based on three independent experiments, ... drafted the manuscript RYZ designed, directed and wrote the manuscript Acknowledgements This study was supported in part by grants from National Institute of Health AI40891 and GM 630 80 (RYZ), and the...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... concentrations were determined by the method of Bradford [ 43] , with BSA as standard Steady-state initial velocity studies were performed at 37 °C and all assays were initiated by adding the enzyme to ... His120 and His145, and the corresponding His101 and His126 in arginase I, were described among the ligands for coordination of Mn2+A and Mn2+B, respectively [9,24] However, the volume of the active ... cluster and, consequently, by a higher concentration of the nucleophilic metal-bound hydroxide [33 35 ] and increased stabilization of the transition state afforded by the more weakly bound metal...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... (3) 10% of pyrene-labeled protein for h at 43 and 60 °C and analyzed polymerization and aggregation induced by the addition of KCl and MgCl2 (Fig 5) As expected, salt addition induced rapid polymerization ... U-actin where D1 - and D2 -actins represent two states of denatured actin and the reversibility or irreversibility of transitions between N, D1 and D2 are unknown Although D1 and D2 states of actin ... independent domains of approximately 11 and 31 kDa Addition of a high concentration of guanidine hydrochloride or urea can reversibly convert D (or partially folded) actin to the completely unfolded...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... Interaction of TnrA with GlnK and GS (Kd3 = ± 0.05 mm) No binding of other nucleotides (ADP, AMP, and GTP) was detectable (Fig S1), confirming their weak effect on the GlnK–TnrA interaction (Fig 3) These ... respectively The ligand (5 lL) was injected 35 times into the 1.4285-mL cell with stirring at 35 0 r.p.m The binding isotherms were calculated from received data, and fitted to a three-site binding model ... and dimethyl pimelimidate dihydrochloride was added to a final concentration of 20 mm The incubation was continued for 30 at 24 °C with gentle shaking The Protein A Sepharose beads were washed...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... 10 mM DTT for h at 25 °C Solid iodoacetamide was then added and alkylation was allowed to proceed in the dark at 25 °C for 30 The reduced and alkylated protein was dialyzed into NaCl/Pi and analyzed ... with mgÆmL)1 of SSAia mixed with a volume of complete Freund’s adjuvant Boosts were administered on day 7, 14 and 28 Sera obtained on day 35 were diluted 10-fold and tested by ELISA and immunoblot ... Blue stained SDS/PAGE, using different concentrations of BSA and then diluted : in M guanidine, 10 mM acetate buffer, pH 4.2, and 10 mM EDTA Denatured b chain was added dropwise to the renaturation...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... both H2O2 and at least one additional ROI were generated during the tyrosinase-mediated oxidations of DOPA and dopamine The identity of the ROI could not be determined by inhibition studies using ... performed with HPLC-ED to determine if, and to what extent, H2O2 was generated during the autoxidations and tyrosinase-mediated oxidations of tyrosine, DOPA, and dopamine This sensitive and specific ... extremely sensitive and specific electrochemical methods detected and quantitatively measured, in real time, the production of H2O2 during tyrosinase-mediate oxidations of the DOPA and dopamine Comparative...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25