isolation and differentiation potential of fibroblast like stromal cells derived from human skin
Báo cáo y học: "Proliferation and differentiation potential of chondrocytes from osteoarthritic patients" ppt
... the average number of cells per milligram of cartilage between the affected and unaffected sides (Table 1) These numbers did not differ from the average number of cells isolated from ACT patients ... flattened cells on the surface and round cells in the centre (Fig 3) Spindle-shaped cells were found in the central part of the pellet in some donors, and the frequency of spindleshaped cells was ... affect the proliferation ability and disappeared after the first change of medium The cells from the unaffected and affected sides expanded with, on average, 0.21 and 0.22 cell doublings per day,...
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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx
... role of PfPNP and its functional relationships with PfMTAP, we carried Table Purification of recombinant purine nucleoside phosphorylase from P furiosus A typical purification from 10 g of wet cells ... responsible for the different substrate specificity of hPNP and hMTAP (Glu201, Asn243 and Val245 of hPNP instead of Ser178, Asp220 and Asp222 of hMTAP, respectively) The last important substitution ... and the elution was carried out with : 95 (v ⁄ v) mixture of 95% methanol and 0.1% trifluoroacetic acid in H2O The retention times of inosine and hypoxantine, guanosine and guanine were 10.5 and...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... pH 7.4, and washed by the same buffer The proteins were eluted in two phases with 300 mL of linear gradient of 0–0.6 M NaCl and 50 mL of linear gradient of 0.6–1 M NaCl at a flow rate of 0.8 mLÆmin)1 ... formation of Fe(II) from the Fe(III)–siderophore complex upon incubation with NADH, succinate and an ultracentrifuged and dialyzed cell-free extract while Dailey and Lascelles [25] noticed reduction of ... calibration of the column with standard proteins of known molecular masses, the molecular masses of native FerA and FerB were estimated as 25 kDa and Table Purification of P denitrificans Fe(III) reductases...
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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx
... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult -derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... ectopic granule -like cells and MF sprouting [52] Hence, neurogenesis is enhanced in the DG and SVZ in animal models of epilepsy and seizure-induced ectopic granule -like cells and MF sprouting ... only from newborn neuronal cells, but also from mature dentate granule cells Immunohistochemistry and confocal microscopy analysis of autopsies for markers of the cell cycle and neuronal differentiation, ...
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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt
... expression, and thus can be a valuable tool in understanding carotenoid metabolism in tomato Methods Isolation and cloning of ShCYC-B promoter Isolation of 5' flanking region of CYC-B gene from S ... gene in S lycopersicum and S habrochaites The mRNA levels of CYC-B gene in leaf and flower, and at different stages of fruit development of S lycopersicum cv Pusa Ruby and S habrochaites genotype ... mature green, breaker, orange and red ripe stages of fruit ripening The images of different stages of fruits were derived from one representative line harboring single copy of transgene for each ShCYC-B...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... muscle actin; hESECs+MCs: a mixture of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hESMCs: MCs derived from human ES cells http://www.translational-medicine.com/content/6/1/54 ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of...
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Báo cáo y học: "MLN51 and GM-CSF involvement in the proliferation of fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis" pps
... development of new RA therapeutics Materials and methods Isolation and establishment of RA FLSs from patients with RA FLS cells (designated RA s-2, 2–6, 2–14, 2–18, 2–36 and 2– 38) were prepared from ... and discussion of the data JJ, YEC and YJ performed most of the studies and were responsible for the execution of most of the experiments SAY and WUK provided the patients' FLS and SF samples, ... OA FLSs resulted from one (2–43) of the three OA FLSs (b) Western blot analysis of MLN51 in FLS samples RA FLSs (2–18, 2–36 and 2–38) and OA FLSs (2– 43, 2–46 and 2–47) isolated from each patient...
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Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx
... Preparation of fibroblast- like synovial cells Explant cultures of FLS cells were prepared from the knee joints of normal rats or from rats days (acute phase) or 20 to 28 days (chronic phase of inflammation) ... different from co-cultures of DRG neurones and FLS cells from normal knee joints) Figure 4a,b displays a cover slip with a co-culture of DRG neurones and FLS cells isolated from knee joint from rats ... the effect of FLS cells from chronically inflamed knee joints on NK1 receptor expression Unlike the presence of FLS cells from chronically inflamed joints, the supernatant of FLS cells from chronically...
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Báo cáo y học: "Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation" pot
... resulting from following comparisons: scaffold and monolayer cultures from ND chondrocytes; scaffold and monolayer cultures from OA chondrocytes; chondrocytes from OA and ND cultured in monolayer; and ... Rosova I, Nolta JA, Myckatyn TM, Sandell LJ, McAlinden A: Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose -derived stromal cells Biochem Biophys Res Commun 2008, ... factors and degradative enzymes could be of interest for the establishment of a non-destructive detection assay to ensure cell and culture quality, identity and purity Increased expression of MMP1 and...
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Tài liệu Medicinal Chemistry and Pharmacological Potential of Fullerenes and Carbon Nanotubes docx
... delivery of TERTsiRNA into tumor cells (murine and human) and silence the TERT gene, which is critical for the development and growth of tumours They observed that treatment of different tumour cells ... already published reports of cellular internalisation using multiple types of cells (fibroblasts, epithelial and cancer cells, phagocytes, bacteria and fungi) in various studies and under different ... shown the delivery of siRNA molecules able to silence the expression of the cellsurface receptors CD4 and co-receptors CXCR4 in human T -cells and peripheral blood mononuclear cells by CNTs Because...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... performed using T-COFFEE [44] Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzed ... 0.2 characterization and Jannaschia sp CCS1 evaluation of HYDJs from Characterization of HYDJs To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH to N-carbamoylp-hydroxyphenylglycine...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... release more than one mole of adenine from each mole of ribosomes in 10 and even up to 12 mol of adenines in 40 (Fig 4B) Base and position specificity of lamjapin in depurination of SRD RNA Synthetic ... 10 lL of a solution of 0.2 M NaCl containing 100 lL of carrier tRNA and 40 lL of ethanol was added to the sample and kept at °C for 60 The mixture was centrifuged (15 000 g for 20 min) and the ... biosynthesis, and still in the range of the IC50 (0.03–4 nM) of type I RIPs The IC90 (the concentration of RIP causing 90% inhibition of translation) of lamjapin in the cell-free system of rabbit...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
... linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolated from the peel of young lemon fruits resulted in the identification of nine putative ... (featuring a Km of 3.1 lM and an apparent Vmax of 28.49 lmolÆh)1Æmg)1) and a substrate inhibition curve (featuring a Km of 13.5 lM, an apparent Vmax of 89.47 lmolÆh)1Æmg)1 and a Ksi of 5.65 lM) were ... picked 1–3 lL of the resulting phagemids was used each time to infect 200 lL of SOLR cells and the next day single colonies were picked from Luria–Bertani plates DNA isolation and sequencing...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... types of plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
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Báo cáo khoa học: Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua) docx
... Institute of Iceland and 50 specimens were caught randomly After killing the fish by a concussion of the brain by striking of the cranium, mucus samples were collected by scraping the skin and then ... Antimicrobial components of cod mucus G Bergsson et al Fig Purification of an antimicrobial component from 12 mg protein ⁄ peptide extract, prepared from skin mucus of cod, by use of HPLC The antimicrobial ... parasin I from histone H2A in the skin mucosa of fish FASEB J 16, 429–431 Cole AM, Weis P & Diamond G (1997) Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions...
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Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx
... immunostaining of apoptotic and differentiated HeLa cells A, C, E and G show control cells, B, D, and F paclitaxel (10 nM for 24 h) treated cells, and H dbcAMP (0.5 mM for 72 h) treated cells In A, B, G and ... Western blot of PP17 variant distribution in HeLa cells and human milk (A) In control cells, small amounts of PP17a, PP17b and PP17c (PP17a dimer) was present in the buoyant fraction and moderate ... purified from milk and its cDNA was isolated from a mammary gland clone collection [57], while human mammary gland and mammary adenocarcinoma ESTs similar to PP17b cDNA was found by BLAST, and subsequently...
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Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx
... of the structural and functional properties of the native proteins and thus may offer a useful basis for the design of agents for therapeutic intervention [7] Studies of different fragments and ... lipid lateral organization of GUVs prepared from surfactant -like lipids in the absence and presence of Ca2+ The yield of individual GUVs was very low in the presence of KL4, and the GUVs formed displayed ... effect of these lipids on the physical properties of the membrane Effect of KL4 on the lipid order of surfactant -like membranes To evaluate KL4 effects on the lipid order of surfactant -like liposomes,...
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Báo cáo sinh học: "The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer’s disease" pdf
... important feature of functional anti-Ab antibody is the binding to all species of Ab42 peptide and inhibition of cytotoxic effect of Ab42 oligomers and fibrils on human neuroblastoma SH-SY5Y cells We ... surface of VLP in a repetitive and ordered array Such organization of the epitope may induce T cell-independent B cell activation and production of anti-Ab antibodies of IgM isotype On the other hand, ... analyses and interpretation of data and statistical analysis She helped to draft the manuscript RC participated in preparation of chimeric viruses, purification of viral proteins and performing of...
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báo cáo hóa học: " Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells" docx
... immunosuppressive activity of standard MSCs despite the appearance of a small number of senescent -like cells Materials and methods Production of MSCs and SB623 cells MSC and SB623 cells were produced ... priming and activating naïve and memory T cells and analyzed on the BD FACS Calibur using CellQuestPro Page of 14 Ex vivo culture of human peripheral blood T cells Human T cells were enriched from ... cells having various features of regulatory T cells SB623 cells are derived from NICD-transfected MSCs and expanded in the absence of exogenous cytokines As such, SB623 cells retained the standard...
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- current status and future potential of nuclide discoveries
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- depletion degradation and recovery potential of estuaries and coastal seas 1806
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- prediction and temporal persistence of psychotic like experiences
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- comparison of neural differentiation potential of human pluripotent stem cell lines using a quantitative neural differentiation protocol
- cellular molecular and biological insight into chemopreventive and therapeutic potential of 3 3 apos diindolylmethane dim
- isolation and functional assessment of mitochondria from small amounts of mouse brain tissue
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