in vitro adult rat adipose tissue derived stromal cell isolation and differentiation
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver ... candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large number of AT-MSCs can be obtained easily with minimal invasiveness by liposuction ... M and dimethyl sulfoxide [13] These cells expressed albumin and a-fetoprotein during differentiation and demonstrated low-density lipoprotein (LDL) uptake and production of urea Further studies...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... support tissue regeneration and minimize tissue damage [30] Engrafted ASCs and SCI-induced chemotactic factors play important roles in proliferation, migration and differentiation of endogenous spinal ... New canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose- derived adult stem cells: Transplantation of ASCs in spinal...
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Investigation of osteogenic characteristics of human adipose derived stromal cells
... promise in tissue repair and regeneration is the adipose tissue Adipose tissue is a mesoderm derivative and contains a varied stromal population, encompassing microvascular endothelial cells and ... adhesion; through their interaction with 11 extracellular matrix proteins like laminins, collagen, vitronectin, they play a key role in regulate cell proliferation and directing differentiation (Klees ... muscle cells and a type of precursor cells called Adipose tissue derived cells (ADSCs) (Zuk PA et al., 2001) These cells have variably been known as Processed Lipoaspirate (PLA), adipose tissue derived...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... hADSCs by using collagenase digestion and adherence screening Isolated hADSCs proliferated at a high rate and maintained a multipotentdifferentiation capacity in vitro for up to 12 passages Since no ... containing 1000 U/ml penicillin and 1000 μg/ml streptomycin to remove contaminating blood cells The specimen was then cut carefully Connective tissue and blood vessels were removed and the tissue ... contribute to tissue engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose- derived mesenchymal stem cells; MSCs: mesenchymal stem cells; ALP: alkaline phosphatase;...
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Characterization of human adipose tissue derived adult multipotent precursor cells
... Chen Fulin Polysaccharides in Tissue Engineering Applications, Handbook of Carbohydrate Engineering In press Intellectual Competition Finalist in Tan Kah Kee Young Inventors Awards 04 Making bone ... scaffold /tissue construct should maintain sufficient structural integrity during the in vitro and/ or in vivo growth and remodelling process (Hutmacher DW 2000), Bioactive materials incorporated into ... counting data for Alizarin Red S and Masson Trichrome stains Fig 8.7 Semi-quantitative stain counting data for Collagen Type I, Osteopontin and Osteonectin staining Fig 8.8 Mechanical testing...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... we reactivated the resting T cells with ionomycin and PMA, and subjected the T cells to ICCS using antibodies against IFN-γ and IL-4 We found that the IFN-γ-producing Th1 cell populations were ... two washes, the cells were resuspended and fixed in 1% paraformaldehyde in PBS and analyzed using a FACSCalibur flow cytometer and the CELLQUEST program (Becton Dickinson) The live cells were gated ... cells co-expressing nuclear lacZ gene were separated from un-transduced cells by staining with fluorescent LacZ substrate and sorted by FACS To label the lacZ-positive cells, we resuspended cells...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... S100 calcium binding protein A3 S100A3 1.92 1.55 Chromosome 17 open reading frame 27 C17orf27 1.63 1.80 RAS and EF-hand domain containing RASEF 2.31 1.71 Integrin, alpha V (vitronectin receptor, ... JAK-STAT signaling; cell adhesion and extracellular matrix-interactions; focal adhesion signaling; and WNT signaling There is a substantial body of work linking Wnt signaling with stemness and malignant ... starting material was limiting cDNA generated from the cell lines P4E6 [47] and PC346C (kindly provided by Nefkens Institute, Erasmus University, Rotterdam) was combined in a 1:1 ratio and used...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... primary cells change during passaging, and cells become increasingly senescent Furthermore, dedifferentiation or transdifferentiation processes can occur during the in vitro culture of primary cells ... BAKs in clinical trials The distinguishing factor between BAKs and hemofiltration devices is the bioreactor unit containing renal cells As explained above, renal proximal tubule -derived cells ... in vitro applications The underlying reason behind such a great interest in tissue models that contain more than one cell type is that a multiple cell type-system enables the study of cells in...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
... Objectives Literature review 2.1 Tissue engineering and regenerative medicine 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter ... showing a general sequence of steps involved in tissue engineering and regenerative medicine strategies Cells are isolated from the donor tissue sections obtained through biopsies which are expanded ... in tissue engineering and regenerative medicine strategies Cells are isolated from the donor tissue sections obtained through biopsies which are expanded in vitro and seeded on 3D cell culture...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... brought into an operating room and placed in a supine position She was then sedated with propofol mg intravenously (push) and a 20 mg/hour rate of continuous infusion After cleaning her abdominal ... Food and Drug Administration: Cell therapy Korean Food and Drug Administration Rules and Regulations Seoul, Korea: Food and Drug Administration;, Chapter 2, Section 12 Arnoczky SP: Building a ... for injection of the mixture into the joint In order to inject the stem cell and PRP mixture, she was first placed in a supine position with her right knee bent at 90° After cleaning with povodine-iodine...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... modulatory cytokines for groups of MDSC-inducing and non-inducing human cancer cell lines [16] These studies suggested multiple mechanisms of MDSC induction amongst tumor cell lines, including inflammatory ... elevated in many of the MDSC-inducing cell lines, particularly ovarian and cervical cancer cell lines, and PGE in combination with GM-CSF induced weak suppressive function in CD33+ cells ([16], ... suppressor cell inducing and non-inducing cell line groups, a linear regression analysis was performed to evaluate for a linear correlation between strength of suppressor cell induction and gene...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... suggested that the defining vital characteristics of a 4- or 8 -cell human embryo must include continued and integrated cellular division, growth, and differentiation [16] And by extension, embryos ... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... using comparative genomic hybridization Hum Genet 2000, 106:210-217 Eckert J, Tao T, Niemann H: Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos...
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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
... properties of cord lining cells using in vitro assays of T lymphocyte activation and dendritic cell maturation To investigate the mechanisms of cord lining cell and T lymphocyte interactions To study ... of cord lining cells in vivo using an immunocompetent mouse model To test the therapeutic application of cord lining cells in ex vivo insulin gene therapy using a streptozocin (STZ) induced type ... depending on their specific cell types According to Drukker’s study, MHC I expression in human ESCs increased during differentiation, both in vitro into embryoid bodies and in vivo into 12 teratomas...
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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
... cells induced for myogenic differentiation 1A) Dystrophin expression in muscle control and in the induced hFTs cells 1B) Skeletal myosin expression in muscle control and in the induced hFTs cells ... resuspended in PBS (Gibco – Invitrogen, Carlsbad, CA) at a concentration of 1.0 × 105 cells/mL and stained with saturating concentration of antibodies After 45 minute incubation in the dark at ... cells 1C) Myosin band observed in the muscle control and in the induced hFTs cells by Ponceau S membrane dyeing 1D) IF assay, indicating dystrophin expression (in green fluorescence) in myotubes...
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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx
... in streptavidin-peroxidase complex (1:500; Dako), rinsed in distilled water, and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma-Aldrich) for minutes and counterstained ... used for cell proliferation rate studies, cell surface epitope characterisation, and cell aggregate culture Cell proliferation rates Cell proliferation rates were measured for passage cells plated ... (αSMA) staining in the infrapatellar fat pad (IPFP) tissue vasculature 3G5, von Willebrand factor (vWF), and alpha smooth muscle actin (αSMA) staining in the infrapatellar fat pad (IPFP) tissue...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... determined at the hypoxanthine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus by plating in medium containing 6-thioguanine (6-TG) or diaminopurine ... differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend the rodent cell studies, and to identify cellular factors in...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... demonstrated that the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding ... medium was drained off The cells were rinsed times for in phosphate buffer and post-fixed in osmium tetroxide for h The samples were then rinsed with distilled water and dehydrated using a graded ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... pancreatic juice and intestine: inadequate for role in zinc absorption Am J Clin Nutr 35, 1–9 Evans GW & Johnson PE (1980) Characterization and quantitation of a zinc binding ligand in human milk ... pathway Indeed, most of the intracellular NAD in liver is synthesized from the amino acid rather than from dietary niacin and the formed dinucleotide is the main vitamin source for extrahepatic tissues ... al hydrophobic index of the residues within the window centered on amino acids involved in metal binding, it can be noticed that regions around His6 and His8 in the human protein are predicted...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... slow-migrating major band and several faster-migrating faint bands Alternative splicing occurs in many genes, and so DNA fragments were purified from the major band and cloned into a sequencing vector ... thick lines indicate intron of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron in fragments C and D, and 3¢ portion of 25 bp in intron in fragments F and I The number at the right of each ... splicing variants initiated from both exons 1a and 1, as shown in Fig 2B Alternative splicing transcripts without exon shift the reading frame and generate a stop codon in exon 4, resulting in...
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