... sacrificed at the end of experiment by cervical dislocation The liver, lung, brain, heart, and spleen were isolated, and organ weights of all mice were measured Preparation of Gold Nanoparticles Gold ... 1064 nm The wavelengths ofthe pump andthe Stokes lasers (Pump = 870 nm and Stokes = 1064 nm) were tuned to match a Raman shift (*2100 cm-1) which falls in the socalled ‘‘silent region’’ ofthe ... Modification of GNP The highly immunogenic peptides pFMDV and pH5N1 were designed and synthesized based on viral protein of foot -and- mouth disease virus type O and matrix protein of influenza A...
... dilution ofthe spores) and then vortexed vigorously for 10 s and incubated at 37°C After 1, and h, 0.5 ml ofthe spore culture was sampled to determine the number of surviving spores The spore ... passes through the stomach of a pig The fact that the viable counts of most ofthe six Bacillus strains did not decrease after h of incubation suggests that a reasonable percentage of these Bacillus ... turbid while the other isolates andthe control did not change On the fifth day, the incubations of isolates B subtilis MA139 and Bacillus spp 634 were sampled and many spores had germinated The tolerance...
... performed most ofthe analysis and compiled the manuscript FC performed part ofthe immunoassays HTK carried out the renal histological analysis RAL contributed to the development of some ofthe assays, ... during the most active stage of disease [29] The discrepancy between the two studies could be due to a number of factors, including the longevity ofthe injected protein andthe level of free ... and sequenced (data not shown) to confirm the presence ofthe wt.DNase I or ash.DNase I construct To confirm the expression and secretion of DNase I from the T cells in the transgenic mice, the...
... and SQH participated in the design ofthe study HMY conceived ofthe study, and participated in its design and coordination GPZ and JL provided some intellectual recommendation and reviewed the ... irradiated the tumor cancer cells simulating the IMRT plans They delivered a total dose of Gy to the cell lines over min, and 20 min, and found that compared with theand group, the survival fraction of ... Page of Figure The effects of different interfraction interval time on the TGDT The mean ± SE of tumor growth time in the group ofthe control is 8.09 ± 0.61 days P < 0.05 as compared with the...
... at the end ofthe reaction The Ct was manually set up at the level that reflected the best kinetic PCR parameters, and melting curves were analyzed The average and standard deviation (SD) ofthe ... cells and participated in the RT-PCR and DNA methylation assay KS participated in the establishment ofthe mouse model system and data analysis JF directed and supervised the experiments and interpretation ... inhibit their transcription and their generation of new viruses Thus, the transcriptional silencing ofthe Tax gene has been studied extensively in terms of DNA methylation ofthe 5'-LTR, which is the...
... Figure The incorporation of GagPol and tRNALys3into wild-type and mutant HIV-1 The incorporation of GagPol and tRNALys3into wild-type and mutant HIV-1 A Schematic showing the deletions made in the ... Gag and homologous sequences in the Gag part of GagPol, the interaction of RT sequences in GagPol with Gag p6 could place the RT-bound tRNALys3 closer to Gag NC sequences and viral RNA in the ... the 5' part ofthe pol gene of Moloney murine leukemia virus blocks proteolytic processing ofthe gag and pol polyproteins J Virol 1985, 53:899-907 Stewart L, Schatz G, Vogt VM: Properties of avian...
... Energy, the Department ofthe Interior, the Department of State, the Department of Transportation andthe Environmental Protection Agency.43 Canada andthe United States in protecting the Arctic ... among them the National Science Foundation, the Department of Commerce (especially the National Oceanic and Atmospheric Administration), the Department of Defense, the Department of Energy, the ... capable of year-round sailings between Alaska andthe east coast ofthe United States The voyage was only the eleventh complete transit ofthe Northwest Passage, andthe first since the end of World...
... areas ofthe Arctic.35 This, then, provided the terms of reference for the PAME working group The scope of its work was defined as the examination ofthe activities/sources of pollution and their ... strengthen recognition ofthe particularly sensitive character of ice-covered parts ofthe Arctic Ocean.73 They also noted the need for strict standards in the transportation of oil in the Arctic, ... of State, 1994), pp 2,016–18 Artand Annex I ofthe Protocol; see the discussion by Joyner, Chapter in this book A Stateofthe Arctic Environment Report, p vii Para 2(3) ofthe 1996 Report of...
... understanding ofthe entity being audited and its environment, including its internal control, sufficient to identify and assess the risks of material misstatement ofthe financial statements whether ... those risks, andthe results thereof Due to the complex nature of certain financial instruments, it is vital that both the entity andthe auditor understand the instruments in which the entity ... gained an understanding ofthe entity and its environment ISA 545 requires the auditor to determine the need to use the work of an expert and, when the use of such an expert is planned, the auditor...
... heating and power generation is considered the main source of SO2 and particulate contamination, which damage human health.52) Domestic sources of SO2 are associated with the use of coal and other ... Occupants of apartment buildings and condominiums with damp problems could have their health affected by microbial contamination.35–37) On the other hand, physiological and psychological effects of ... MATERIALS In 2003, the Ministry of Land, Infrastructure and Transport of Japan amended the Building Standard Law to control indoor chemical concentrations This regulation restricts the use of formaldehydeemitting...
... according to theof cial nomenclature [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, ... value of each set The sum ofthe scores of each wing is divided by the number of limbs in each set approximately 15 It should also be noted that S-4o9cDH-RA did not evoke the loss of hand plate ... the expression of shh is restricted to the posterior half ofthe limb buds However, both retinoids induced the expression of shh in the anterior section As there is no endogenous expression of...
... andthe EGF receptor at high levels, we performed a comparative analysis, in time studies, ofthe in vivo effects of insulin and EGF on the contents of Grb7 and Grb14 in the plasma membrane and ... homology (PH) and SH2] or phosphorylated insulin receptor-interacting region (PIR) [6] and inhibits the tyrosine kinase activity ofthe IR [7] On the basis ofthe crystal structure ofthe tyrosine ... on the role of this adaptor protein The endosomal colocalization and ⁄ or cointernalization ofthe activated IR and Grb14 raises the question as to whether Grb14 could, directly or via its partners,...
... helices and at the junction crossover – andthe unpairing of four base-pairs in the centre ofthe junctions [19] Detailed understanding ofthe functional role of these interactions would require the ... Binding of CCE1 results in the complete unpairing ofthe four base-pairs in the centre ofthe junctions with a lesser disruption ofthe next base-pairs [19], which is reminiscent ofthe structure of ... positioning ofthe SAP motifs in relation to the rest ofthe protein as well as for the stability ofthe protein The truncated Ydc2 mutant proteins localize to the mitochondria in S pombe Fig Binding of...
... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability ofthe structure ofthe protein The difference ofthe increments ... on the surface On the other hand, anionic surfactants such as SDS and LAS bind to the surface regions of protein, andthe surface regions would be unfolded and removed from the core structure of ... 60% ofthe original activity after h, and 30% after h The D266NALP I showed 40 and 20% ofthe original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and...
... move the picture to the side, and then push another sequence of buttons to open the safe One floor tile is alarmed, and players lose the game if they step on this tile without deactivating the ... separates the user andthe NLG system and connects them over the Internet To achieve this, the GIVE software infrastructure consists of three components: the client, which displays the 3D world ... sequence of actions leading to the goal When a user starts the client, it connects over the Internet to the Matchmaker The Matchmaker then selects a game world and an NLG server at random, and requests...
... anti-GroEL Ig [27] The high yield ofthe UP12-GroEL complex isolationandthe ATP-mediated dissociation ofthe complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact ... GroEL from 15 and 30 lg ofthe cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) ofthe cell extract ... recognition site and two flanking unrelated short sequences The DNA sequencing andthe deoxyoligonucleotide synthesis were performed by the Scientific Services Department ofthe Weizmann Institute of Science...
... value); Vt is the value ofthe indicator i in the year t; Vp is the prospective value ofthe indicator i ofthe last year ofthe plan (minimum value) The UNDP’s HPI is an indicator of poverty in ... assess the quality ofthe water andthe likelihood of diarrhoea, the answer it provides should not change (randomly or not) every time that the test is run on the same sample; Valid or relevant: the ... poverty andthe poor who live in those areas the environmental poor The environmental poverty 3.1 The categories ofthe environmental poverty from ADB’s point of view The concept of poverty of ADB,...
... Criteria, and in 2004 on the PART The five agencies are the Department of Defense, the Department of Energy, the National Aeronautics and Space Administration, the Department of Health and Human ... reputation ofthe first agency and diminish the credibility ofthe evaluation process itself Because the framework of PART is virtually the same for all agencies and because the principles of scientific ... scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare Upon the authority ofthe charter granted to it by the Congress...
... management andthe promotion process, recruiting, the mix of officers and civilians in the department, and morale (For more on the RAND analysis, see Rostker, Hix, and Wilson, 2007.) Prior to the RAND ... [RAND] partnership [was] to get the leadership ofthe city to really understand the needs ofthe police department,” Riley told the summit The mayor andthe city council, hearing it from RAND’s ... between the taking of an exam and a promotion, during which time some ambitious and promising officers left the department Such delays often meant the department had only those at the bottom of the...
... activation of these genes within the same time window The activator-binding domains in the Swi1 and Snf5 subunits ofthe SWI ⁄ SNF complex are essential for recruitment ofthe SWI ⁄ SNF complex to these ... properties ofthe SWI ⁄ SNF complex, but the phenotype of a mutant lacking both domains differs from the wild-type [33] Thus, it is possible that the phenotypic defects are a result ofthe inability of ... for understanding the roles played by the different SWI ⁄ SNF subunits and, from the results obtained in the present study, we can now add that other regions of SWI ⁄ SNF cannot replace the recruiting...