immunophenotype and differentiation potential of msc

... to 10 ng of RNA, 15 µl of TaqMan Universal PCR master mixture (Applied Biosystems), 1× assayon-demand mixes of primers and TaqMan MGB probes All samples were analysed in triplicate and PCR was ... stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The added serum contains high levels of growth factors released by cells and platelets ... blue/van Gieson stain and the presence of type II collagen is indicated by red staining within the scaffold Panels (b), (e) and (h) are higher magnifications of portions of (a), (d) and (g), respectively...

Ngày tải lên: 09/08/2014, 06:22

... factors and degradative enzymes could be of interest for the establishment of a non-destructive detection assay to ensure cell and culture quality, identity and purity Increased expression of MMP1 and ... comprehensive molecular analysis of the differentiation process of OA Page of 14 (page number not for citation purposes) chondrocytes, expanded chondrocytes and chondrocytes in scaffold cultures ... observations of each assessed ND and OA sample were averaged and used for statistical analysis Differentiation was also studied by immunohistochemical localization of collagen types I and II as...

Ngày tải lên: 09/08/2014, 14:22

... considered both one of the most promising applications and one of the most studied since the publication of the fundamental works of Dugan and coworkers, who analyzed fullerene capability of scavenging ... with diameters in the range of 0.5–1.5 nm and 2–100 nm and lengths in the range of 20–1,000 nm, and 1–50 μm for SWNTs and MWNTs, respectively 2 Biomedical Applications of Functionalised Carbon ... Milan, Italy Volume 1: Medicinal Chemistry and Pharmacological Potential of Fullerenes and Carbon Nanotubes Volume Editors Dr Prof Franco Cataldo Director of Lupi Chemical Research Institute Via...

Ngày tải lên: 17/02/2014, 05:20

... perilipins and adipophilin, the latter of which was also involved in adipose cell differentiation Taken altogether, a comparison of PP17b and its gene to perilipins and adipophilin, members of the ... splicing of PP17 variants, the importance of protein kinase A (PKA)- and protein kinase C (PKC)dependent pathways for the regulation of PP17 gene expression was demonstrated, and the effect of the ... the phase of cell cycle, differentiation and apoptosis on expression of this gene was also studied Furthermore, evidence was presented for the association of PP17b to lipid droplets and milk lipid...

Ngày tải lên: 23/03/2014, 21:20

... surface of VLP in a repetitive and ordered array Such organization of the epitope may induce T cell-independent B cell activation and production of anti-Ab antibodies of IgM isotype On the other hand, ... analyses and interpretation of data and statistical analysis She helped to draft the manuscript RC participated in preparation of chimeric viruses, purification of viral proteins and performing of ... Detection of anti-Ab and anti-HA antibody responses using ELISA Concentration of anti-Ab antibody in sera of immunized and control mice was measured as described previously [21] Briefly, wells of 96-well...

Ngày tải lên: 18/06/2014, 22:20

... beginning of the steep increase of Fo (Fig 1), [4] 2.3 Seasonal time course of thermostability of photochemistry During 2000, Tc was recorded four times (beginning of April, beginning of May, end of ... (beginning of April, beginning of May, end of August and end of October, i.e., days of year 109, 138, 241, and 297) on needles of potted seedlings of C sempervirens, C atlantica, P halepensis et P ... seedlings of cedar (Cedrus atlantica and C libani), J Exp Bot 48 (1997) 1835–1841 [8] Epron D., The temperature dependence of photoinhibition in leaves of Phaseolus vulgaris L Influence of CO2 and...

Ngày tải lên: 08/08/2014, 01:22

... and confocal microscopy analysis of autopsies for markers of the cell cycle and neuronal differentiation, like proliferating cell nuclear antigen and β-tubulin, show that cell proliferation and ... in a broad range of neurological diseases and disorders (fig 1) The contribution and significance of this modulation to the etiology and pathogenesis of neurological diseases and disorders remain ... degenerating regions of the CNS [65] Some of the data observed by mean of immunohistochemistry for cell cycle proteins and BrdU labeling in the brains of AD patients and in animal models of the disease,...

Ngày tải lên: 08/08/2014, 17:20

... Inhibition of c-Src upregulates maspin transcription and protein levels 70 Figure 33: Model of c-Src phosphorylation of Mdm2 and its downstream effects 76 x Figure 34: Analysis of Mdm2 and p53 protein ... protein levels in shGFP and shMdm2 TMD-231 cells 81 Figure 35: Growth and cell cycle analysis of TMD-231shGFP and shMdm2 cells 82 Figure 36: In vitro invasion potential of shGFP and shMdm2 TMD-231 ... chemotherapeutics, and thus help to activate p53 (34) There are numerous splice variants of mdm2 mRNA and multiple isoforms of the Mdm2 protein have been identified in tumors and normal tissues (35) Two of these...

Ngày tải lên: 24/08/2014, 09:46

... shrinking of cytoplasm and Begins with swelling of cytoplasm and condensation of nucleus mitochondria Blebbing of plasma membrane without loss Loss of membrane integrity of integrity Aggregation of ... evasion of programmed cell death, limitless replicative potential, sustained angiogenesis, and tissue invasion and metastasis” (Hanahan and Weinberg 2000) Cancer affects all parts of the body and ... successful development of CDDO and its derivatives ascertains the importance of the rich pool of triterpenoids serving as valuable sources of precursors and compounds for development into potential anticancer...

Ngày tải lên: 11/09/2015, 10:01

... culturing and activation of dendritic cells (DCs) 64 CD4+ T cell selection and proliferation 65 Molecular biology 66 2.3.1 Design and synthesis of EV71-specific primers and probes 66 2.3.2 Design and ... vaccine candidate is of great interest as it provides a better understanding of the mechanisms involved in protective immunity and may therefore help in the design of effective vaccines and diagnostic ... Isolation of plasmid DNA 72 72 2.3.4.1.1 Preparation of plasmids by the modified alkaline lysis method of Birnbolm and Doly (1979) 72 2.3.4.1.2 Preparation of plasmids by the modified boiling method of...

Ngày tải lên: 12/09/2015, 08:19

... values of 0.09 and 0.18 for hSGLT1 and hSGLT2, respectively These data clearly show the electrogenic uptake of thioglycoside I and VII across the plasma membrane Discussion Figure Correlation of ... membrane potential induced by D-glucose, thioglycosides I and VII and phlorizin As expected, the maximal effect was observed with D-glucose with FRET values of 5.62 and 2.29 for hSGLT1 and hSGLT2, ... therapies and emerging targets for the treatment of diabetes Current Pharmaceutical Design 2001; 7: 417-450 Wood IS, and Trayhurn P Glucose transporters (GLUT and SGLT): expanded families of sugar...

Ngày tải lên: 26/10/2012, 10:04

... During extrusion, a screw speed of the extruder of 100 rpm and a barrel temperature of 100°C was maintained [28] 2.3 Sample collection Samples of yard waste compost (YWC) and finished yard waste compost ... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using Microsoft Excel and results were presented as average ± SD Results and discussion...

Ngày tải lên: 05/09/2013, 16:11

... of all oils tested The main component of one of the oils is pulegone The other is not yet identified and it is called SEM76 (Shaaya and Kostyukovsky, 2006) In our study of the mode of action of ... because of the pesticidal properties of these chemicals (Fenwick at al., 1983) and because of the potential use of methyl ITC as fumigant for wheat (Ducom, 1994) In our study on the rates of sorption ... mortality of all adults tested at very low concentrations of 0.5 μL·L−1 air and exposure time of 24 h A higher concentration of μL·L−1 air was needed to kill larvae of Tribolium, Trogoderma, and Plodia...

Ngày tải lên: 25/10/2013, 05:20

... some of the weaknesses and problems that can affect the quality of studies investigating the potential causes of cryptorchidism and hypospadias Quality assessment of the various studies and of ... prevalence, and evidence for changing incidence, of human TDS disorders; and (2) the range of TDS-like effects of ECs and EC mixtures in animal studies, together with new understanding about when and ... father’s nose; Sperm and egg; Baby’s face; Father and son at sunset [Andrew Penner]; all courtesy of [©iStockphoto.com] Male Reproductive Health Disorders and the Potential Role of Environmental...

Ngày tải lên: 13/02/2014, 10:20

... and the percentage of the oxidative derivative of the monoester and of MIDP at the same doses were, respectively, 25 and 30%, 14 and 26%, and 13 and 13% The data suggest a metabolic scheme comparable ... reduced on pnd and in all treated groups, and also on pnd and at weaning in the high-dose group An unusually high incidence of pup deaths in 4, 2, and litters of the low-, mid-, and highdose groups ... gestational doses of 127–151 mg/kg bw/day and higher and a lactational dose of 166−377 mg/kg bw/day and higher; the developmental NOAEL is in the range of 38–44 mg/kg bw/day (gestational) and 52−114...

Ngày tải lên: 13/02/2014, 10:20

... opportunities and their merits and limitation Moreover, the nature of gearing and potential effected of high gearing on perceived risk and cost of capital I-Analysis of the reasons behind takeovers and ... not reliable NPV must be used for this type of project III- The nature of gearing and the potential effects of high gearing on perceived risk and cost of capital 11 There are some ways to increase ... corporate gearing Two of these, the advantage of debt being ‘cheaper’ than equity and the disadvantage of the cost of equity rising because of increase financial risk, exactly offset each other This...

Ngày tải lên: 17/02/2014, 13:02

... mutant enzymes, F133N and F133A, were expressed, purified and characterized With mm UMP and ATP as substrates, the activities of F133N and F133A were only 50 and 20% of that of the wild-type enzyme ... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... Glu204 (Fig 3A,C), and a hydrophobic interaction between Thr131 and Phe133 (Fig 3D) The central channel of the hexamer is made up of two layers of electrostatic forces on top of each other, with...

Ngày tải lên: 18/02/2014, 16:20

... longitudinal and radial directions of one, three, and five year old bamboo and determine the correlation between SG and bending properties, and (5) compressive strength of bamboo at different ages and ... [Leithoff and Peek 2001] Since the amount of each chemical composition of bamboo varies with age, height, and layer, the chemical compositions of bamboo are correlated with its physical and mechanical ... Department of Science and Technology Misra, M.K., K.W Ragland, and A.J Baker 1993 Wood ash composition as a function of furnace temperature Biomass and bioenergy 4(2): 103-116 Mathew, G and K.S.S...

Ngày tải lên: 22/02/2014, 09:20