fluorophore selection for single molecule fluorescence spectroscopy smfs and photobleaching pathways
Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... trapping and singlemolecule fluorescence supply information beyond what can be learned with either technique alone, permitting structure and mechanics to be probed within a single macromolecule ... wave (singlesided total internal reflection fluorescence, TIRF) Prior attempts to marry optical trapping with single- molecule fluorescence failed to achieve spatially coincident fluorescence and ... simultaneous singlemolecule fluorescence and optical trapping The choices of dyes and excitation wavelengths were optimized to minimize two-photon excitation and achieve acceptably long fluorescence...
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Báo cáo sinh học: "Efficient unfolding pattern recognition in single molecule force spectroscopy data" pps
... as Punias and Hooke Conclusions Single- molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells SMFS can analyze membrane proteins in ... proteins Bioinformatics (Oxford, England) 2010 Bosshart PD, Casagrande F, Frederix PLTM, Ratera M, Bippes CA, Mueller DJ, Palacin M, Engel A, Fotiadis D: High-throughput single- molecule force spectroscopy ... F, Brucale M, Gomez-Casado A, Samori B: Hooke: an open software platform for force spectroscopy Bioinformatics (Oxford, England) 2009, 25(11):1428-30 13 Müller DJ, Sass HJ, Müller SA, Büldt G,...
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POSSIBLE USE OF DNAS IN SINGLE MOLECULE FORCE SPECTROSCOPY TO PROBE SINGLE PROTEIN UNFOLDING SIGNATURES
... characterizing the “relevant” unfolding structures under force for single molecule -Catenin and 2) determining unfolding rates as a function of force for single molecule -Catenin A description of a chemical ... were first done only with DNAs and without proteins, and the results showed encouraging features which were important for its efficient use in single molecule force spectroscopy: 1) short tip-surface ... error in force measurements For Magnetic Tweezers, the force calibration is actually more subtle than discussed previously and could lead to more serious overestimation of the force for short molecules...
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Báo cáo sinh học: " Combined single-molecule force and fluorescence measurements for biology" ppt
... existing single- molecule techniques, in particular those that measure optical and mechanical signals Single- molecule fluorescence Single -fluorophore detection entails the illumination of a molecule ... individual molecules inside living cells [16-18] Single- molecule forces Two principal methods are available for resolving the forces exerted by single molecules [19], optical traps (or tweezers) and ... single- molecule force and fluorescence measurements, and in the foreseeable future this is likely to be the major barrier to non-specialists New families of fluorescence probes and new strategies for...
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Báo cáo hóa học: " Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level" ppt
... imaged For example, the group of Steven Chu [21] visualized single tethered DNA molecules stretched in a uniform flow to investigate viscous forces and hydrodynamic interactions between the DNA molecule ... We have reviewed different methods of stretching and immobilizing DNA molecules for studying single- molecule DNA–protein interactions Single- molecule experiments overcome the problem of ensemble-averaging ... (WLC) model [13, 14], and the information on the force applied to the DNA can be directly converted into extension, and vice versa Methods of stretching and immobilizing DNA molecules have been...
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Efficient core selection for multicast routing in mobile ad hoc networks
... abandon the role query IGMPv2 support backward compatibility with IGMPv1 Code for the type of query and report messages of IGMPv2 and IGMPv1 are the same as 0x11 and 0x12 This allows hosts and ... ad hoc networks maintain only approximate and minimal topology information, and therefore designing a center selection heuristics require more precise information than what is available The median ... children and therefore not mesh created multicast message types c) The node at the periphery of the grid: The nodes are more likely to move in and out by the mobile network, and therefore create...
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Tài liệu Data Center—Site Selection for Business Continuance ppt
... comes to a standstill until the single data center is rebuilt and the applications and data are restored OL-14895-01 Chapter 1—Site Selection Overview Business Goals and Requirements Before going ... loss of data and its full recovery and availability for use For example, if data from a sales order is lost, it represents a loss for the business unless the information is recovered and processed ... Using IGP and BGP In a hot standby solution, both sites are active Each site is active for certain applications and acts as a standby for applications which are not active on that site Hot Standby...
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Báo cáo "Photochemical smog introduction and episode selection for the ground-level ozone in Hanoi, Vietnam " docx
... leading to O3 formation and build up Photochemical smog pollution Smog is a synchrony of two words - smoke and fog Smog can be of two types - industrial or winter smog (e.g London smog) and photochemical ... acid (HCOOH), and other trace substances They are collectively termed photochemical oxidants with ozone and PAN, and are present in the highest concentrations In addition, the aerosols formed during ... can irritate the eyes, causing them to water and sting 2.1 Condition for photochemical smog development of Certain conditions are required for the formation of photochemical smog These conditions...
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Gene Selection for Cancer Classification using Support Vector Machines pot
... corresponds to a single experiment for SVM For MSE, the dashed and solid lines overlap For LDA we could not compute the dashed line, for computational reasons The baseline method performs slightly ... features (thousands of genes) and a small number of training patterns (dozens of patients) They integrate pattern selection and feature selection in a single consistent framework We proposed and applied ... and clustering (Ben-Hur, 2000) It is not limited to RFE type of search It can be used, for instance, for forward feature selection (progressively adding features), a strategy recently used, for...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... CDRs of heavy chain and CDR3 of light chain [ref 39] Fv Randomization in CDR loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local ... A-domain Target molecule Target molecule (XGGGS)n Target molecule Fig Selection of multispecific binders with multiple binding sites for different epitopes The red loops are randomized to select ... design of and screening for bispecific high-affinity proteins recognizing different epitopes in a target molecule The recent explosive increase in new genomic and protein structural information...
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USE OF MINIMAL LEXICAL CONCEPTUAL STRUCTURES FOR SINGLE-DOCUMENT SUMMARIZATION doc
... at Maryland: Translation Selection and Document Selection , Proceedings of the Interactive track for the Cross-Language Evaluation Forum Workshop, Trondheim, Norway, 2003 Nizar Habash and Bonnie ... a license for English and Spanish parsers Update the host/port in the files fdges-client.pl (for Spanish) and fdgen-client.pl (for English) The current values should look like this for fdges-client.pl: ... information management and processing systems, which facilitates laymen and professionals in accessing information that is authored in a language they not understand PLANS FOR THE NEXT YEAR, IF...
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Báo cáo khoa học: "General-to-Specific Model Selection for Subcategorization Preference*" pot
... data and is used in the procedure of section 4.4, and iii) the test data C ts which consists of positive and negative data and is used for evaluating the selected models in terms of the performance ... number of the active feature set is 126 for "Generalto-Specific" method and 800 for "One-by-one Feature Adding" method Therefore, general-tospecific feature selection algorithm achieves significant ... Now, we assume that S contains n feature functions For each feature fi(E S), the sets Vzi and Vyi indicate the sets of the values of x and y for that feature According to those sets, each feature...
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Laser Fluorescence Spectroscopy with 5-Aminolevulinic Acid in Operative Gynecology pdf
... 2004 1213 the one hand, benign cysts and cystomas and, on the other hand, ovarian cancer and borderline ovarian tumors and to diagnose micrometastases in the ovary, peritoneum, and omentum In the ... peritoneum, and greater omentum, on the one hand, and the normal LASER PHYSICS Vol 14 No 2004 unchanged tissues, on the other hand The method under consideration demonstrated 100% sensitivity and specificity ... study, and the detecting and measuring system is used to detect fluorescence and scattered laser light and to transmit it to the detector), (iii) a set of color filters with a transmission band of...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... and C Sinquin for the extraction and the preparation of the fucoidan fraction and for their experimental advices We are grateful to Professor G J Arlaud for his generous gift of purified C1q and ... protein For this purpose we took advantage of the binding properties of C1q toward DNA and of an emerging technique allowing the molecular combing of DNA strands and its observation by single- molecule ... P., Li, H & Fernandez, J (2001) Fingerprinting polysaccharides with single- molecule atomic force microscopy Nat Biotechnol 19, 258–262 11 Camesano, T & Wilkinson, K (2001) Single molecule study...
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Báo cáo khoa học: "Data point selection for cross-language adaptation of dependency parsers" pot
... Data point selection The key idea in our experiments is that we can use a simple form of instance weighting, similar to what is often used for correcting sample selection bias or for domain adaptation, ... part-of-speech, and the first three as fine-grained part-of-speech 683 pings for Arabic, Bulgarian, Czech, Danish, Portuguese and Swedish We only use four of these treebanks, since Bulgarian and Czech ... reestimate parameters in EM performs even better The authors report good results (65%-70%) for somewhat related languages, training on English and testing on German and Spanish, but they modified...
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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx
... collapse[42] and the dynamics of protein folding.[47] Therefore, in order to determine the role of solvent hydrogen bonds and hydrogen bond strength in protein folding, we use single- molecule force spectroscopy ... unfolding events measured using force-clamp spectroscopy for I27 in D2O for constant force measurements at 200 pN, 180 pN, 160 pN and 140 pN Extending a polyprotein at constant force gives a very different ... of b-strands A’ and G for unfolding the I27 protein in D2O The pulling coordinate for the separating b-strands is defined as the distance between the first amino acid of strand A’ (Y9) and the...
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Báo cáo khoa học: "Convolution Kernels with Feature Selection for Natural Language Processing Tasks" docx
... correlation coefficient and mutual information (Rogati and Yang, 2002) reported that chi-squared feature selection is the most effective method for text classification Following this information, we use ... question type We evaluated the performance by using data provided by (Li and Roth, 2002) for English and (Suzuki et al., 2003b) for Japanese question classification and followed the experimental setting ... LOCATION, NUMEX, ORGANIZATION, and TIME TOP for JQA, and “coarse” and “fine” classes for EQC We used the one-vs-rest classifier of SVM as the multi-class classification method for EQC Figure shows examples...
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Báo cáo khoa học: "Active Sample Selection for Named Entity Transliteration" pptx
... network weights without training and training with weights The results clearly show that using weights for training improve the classifier’s performance for both Russian and Hebrew It can also be observed ... Linear concepts and hidden variables ML, 42 A Klementiev and D Roth 2006 Weakly supervised named entity transliteration and discovery from multilingual comparable corpora In ACL K Knight and J Graehl ... classifier’s confidence score is used for ranking of positively tagged transliteration candidates Our initial feature extraction scheme follows the one presented in (Klementiev and Roth, 2006), in which...
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dynamical symmetries for nanostructures implicit symmetries in single-electron transport through real and artificial molecules
... levels for SO(6) dynamical symmetry group (triplet T and three singlets S, E2 , E3 ); (b) the same for SO(5) group (triplet T and two singlets S, E2 ); (c) the same for SO(4) group (triplet T and singlet ... −I (2.30) which form the algebra of the conformal group SO(4, 2) (see Section 9.2.2) and obey the commutation relations (9.32) 14 HIDDEN AND DYNAMICAL SYMMETRIES OF ATOMS AND MOLECULES The operators ... (ground state S and exciton E2 ) and the spin triplet T , 20 HIDDEN AND DYNAMICAL SYMMETRIES OF ATOMS AND MOLECULES Fig 2.2(b) In this case, three irreducible vectors S, R1 , R2 and one scalar...
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- immobilization of proteins for single molecule fluorescence resonance energy transfer measurements of conformation and dynam
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