fluorophore labeling for single molecule fluorescence spectroscopy smfs
Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
Ngày tải lên :
06/08/2014, 18:20
... evanescent wave (singlesided total internal reflection fluorescence, TIRF) Prior attempts to marry optical trapping with single- molecule fluorescence failed to achieve spatially coincident fluorescence ... trapping and singlemolecule fluorescence supply information beyond what can be learned with either technique alone, permitting structure and mechanics to be probed within a single macromolecule Traces ... simultaneous optical trapping and single- molecule fluorescence measurements supply information about the sequence of molecular events Relative timing information can be used, for example, to establish...
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Báo cáo sinh học: "Efficient unfolding pattern recognition in single molecule force spectroscopy data" pps
Ngày tải lên :
12/08/2014, 17:20
... effective as Punias and Hooke Conclusions Single- molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells SMFS can analyze membrane proteins ... Benedetti F, Brucale M, Gomez-Casado A, Samori B: Hooke: an open software platform for force spectroscopy Bioinformatics (Oxford, England) 2009, 25(11):1428-30 13 Müller DJ, Sass HJ, Müller SA, Büldt ... proteins Bioinformatics (Oxford, England) 2010 Bosshart PD, Casagrande F, Frederix PLTM, Ratera M, Bippes CA, Mueller DJ, Palacin M, Engel A, Fotiadis D: High-throughput single- molecule force spectroscopy...
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POSSIBLE USE OF DNAS IN SINGLE MOLECULE FORCE SPECTROSCOPY TO PROBE SINGLE PROTEIN UNFOLDING SIGNATURES
Ngày tải lên :
01/10/2015, 17:27
... characterizing the “relevant” unfolding structures under force for single molecule -Catenin and 2) determining unfolding rates as a function of force for single molecule -Catenin A description of a chemical ... very small forces ( ( ) on this macromolecule ) and see how it reacts This is to simulate typical forces experienced by molecules in our cells To this, we used two different single- molecule techniques ... error in force measurements For Magnetic Tweezers, the force calibration is actually more subtle than discussed previously and could lead to more serious overestimation of the force for short molecules...
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Báo cáo sinh học: " Combined single-molecule force and fluorescence measurements for biology" ppt
Ngày tải lên :
06/08/2014, 18:20
... existing single- molecule techniques, in particular those that measure optical and mechanical signals Single- molecule fluorescence Single -fluorophore detection entails the illumination of a molecule ... the behavior of individual molecules inside living cells [16-18] Single- molecule forces Two principal methods are available for resolving the forces exerted by single molecules [19], optical traps ... sensitivity of single- molecule studies makes them ideal for studying systems in which the event or molecule of interest is rare, as is the case for molecules present at only a single copy or...
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Báo cáo hóa học: " Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level" ppt
Ngày tải lên :
22/06/2014, 18:20
... imaged For example, the group of Steven Chu [21] visualized single tethered DNA molecules stretched in a uniform flow to investigate viscous forces and hydrodynamic interactions between the DNA molecule ... reviewed different methods of stretching and immobilizing DNA molecules for studying single- molecule DNA–protein interactions Single- molecule experiments overcome the problem of ensemble-averaging ... (‘‘GATATC’’ for EcoRV and ‘‘GGATCC’’ for BamHI) which was registered by a sudden drop in the tension This study showed that the cleavage rate for EcoRV decreases with DNA tension whereas that for BamHI...
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USE OF MINIMAL LEXICAL CONCEPTUAL STRUCTURES FOR SINGLE-DOCUMENT SUMMARIZATION doc
Ngày tải lên :
07/03/2014, 11:20
... a license for English and Spanish parsers Update the host/port in the files fdges-client.pl (for Spanish) and fdgen-client.pl (for English) The current values should look like this for fdges-client.pl: ... Automatic Generation of Informative Cross-Lingual Headlines for Text and Speech Thesis Proposal, University of Maryland, 2003 5.3 OTHER PRODUCTS Trimmer: Trimmer generates a headline for a news story ... capabilities useful for intelligence analysts, such as cross-lingual summarization and data mining 6.3 CONTRIBUTIONS TO RESOURCES FOR RESEARCH This work provides an integral part for many NLP applications...
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Báo cáo khoa học: "Semantic Role Labeling for Coreference Resolution" pot
Ngày tải lên :
08/03/2014, 21:20
... overall performance Table shows the performance variations obtained by leaving out each feature in turn Again, it can be seen that removing both I and J SEMROLE induces a relatively high performance ... label to a RE only in case the two phrases share the same head Labels have the form “ARG1 pred1 ARGn predn ” for n semantic roles filled by a constituent, where each semantic argument label ... +SRL role 3.1 argument- Experiments Performance Metrics We report in the following tables the MUC score (Vilain et al., 1995) Scores in Table are computed for all noun phrases appearing in either...
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Laser Fluorescence Spectroscopy with 5-Aminolevulinic Acid in Operative Gynecology pdf
Ngày tải lên :
14/03/2014, 14:20
... light for a short period of time For comparative analysis, we perform a preoperative measurement of the accumulation of the Alasens-induced PP IX in the skin of the internal surface of the forearm ... Pathologies For endometrial cancer (n = 11), the mean fluorescence coefficient is kf = 37.3 For the local form of byendometrial cancer, the diagnostic contrast coefficient is kdc = 5.9 (Fig 3) For the ... spectrum analyzer allowing for fast spectral measurements, and (v) a PC The data obtained are processed using original computer codes Method for Intraoperative FD Patients were informed about the procedure...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
Ngày tải lên :
16/03/2014, 23:20
... with single- molecule atomic force microscopy Nat Biotechnol 19, 258–262 11 Camesano, T & Wilkinson, K (2001) Single molecule study of xanthan conformation using atomic force microscopy Biomacromolecules ... R.M (1999) Single- molecule biomechanics with optical methods Science 238, 1689–1695 Ludwig, M., Rief, M., Schmidt, L., Li, H., Oesterhelt, F & Gautel, M (1999) AFM, a tool for single- molecule experiments ... bind several polyanionic molecules like sulfated polysaccharides and also DNA, but with the opposite effects of either inhibition or activation, respectively Using single- molecule observation of...
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Báo cáo khoa học: "Semantic Discourse Segmentation and Labeling for Route Instructions" potx
Ngày tải lên :
17/03/2014, 04:20
... DECODE PATH (x, n, start, go) for each label y1 node[0][y1 ].cost ← s( y1 , ) node[0][y1 ].end ← start; for j ← to n − for each label yj+1 bestc ← −∞; end ← null; for each label yj cost ← node[j][yj ... mentions of LEFT/RIGHT, • For each occurrence of LEFT/RIGHT, look for an ordinal number, LAST, or END (= end of the hallway) nearby, • Also, for each LEFT/RIGHT, look for a mention of DOOR If ... exactly once for each episode For example, in ”go straight and make the first left you can, then go into the first door on the right side and stop” , LEFT and FIRST occur exactly once for the first...
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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx
Ngày tải lên :
22/03/2014, 18:20
... folding.[47] Therefore, in order to determine the role of solvent hydrogen bonds and hydrogen bond strength in protein folding, we use single- molecule force spectroscopy to measure the force-dependent ... of unfolding events measured using force-clamp spectroscopy for I27 in H2O for constant force measurements at 200 pN, 180 pN, 160 pN, 140 pN and 120 pN C) Force-clamp unfolding of I27 in D2O ... unfolding events measured using force-clamp spectroscopy for I27 in D2O for constant force measurements at 200 pN, 180 pN, 160 pN and 140 pN Extending a polyprotein at constant force gives a very different...
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Báo cáo khoa học: "Japanese Dependency Parsing Using Sequential Labeling for Semi-spoken Language" ppt
Ngày tải lên :
31/03/2014, 01:20
... segments for which dependencies are already determined and which no other segments depend on CaboCha constructs a tree structure by repeating the above process 2.2 Sequential Labeling Sequential labeling ... it is applied to, for example, English part-of-speech tagging and named entity recognition Hidden Markov models or conditional random fields (Lafferty et al., 2001) are used for labeling In this ... of parsed tree Table 1: Label List Used by Sequential Labeling (Window Size: 2) other sequential labeling methods However, our sequential labeling has the following characteristics since this...
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báo cáo hóa học:" Emerging applications of fluorescence spectroscopy in medical microbiology field" potx
Ngày tải lên :
18/06/2014, 15:20
... fluorescent molecule, it is possible to study the complex formation between a small fluorescent labeled and a big unlabelled molecule [7] Fluorescence correlation spectroscopy (FCS) Fluorescence ... sensitivity and specificity for microorganisms associated diseases diagnosis with the help of spectroscopic fingerprints Also, Fluorescence spectroscopy and Fluorescence correlation spectroscopy (FCS) ... technique At present, Fluorescence spectroscopy is being applied in medical microbiology field for various purposes There are many studies which indicate that Fluorescence spectroscopy is promising...
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Báo cáo hóa học: " Record Endurance for Single-Walled Carbon Nanotube–Based Memory Cell" pot
Ngày tải lên :
21/06/2014, 08:20
... charge For the latter reason, low pressure favours current stability as can be seen by comparing the IDS–t curves at 760 Torr and 10-4 Torr [13, 23] This suggests that for better device performance ... ever reported for SWCNT memory is 1.8 104 cycles [22] before device failure Our devices showed remarkable endurance beyond 105 cycles in air, which can be considered as a breakthrough for CNT memory ... with the same fabrication process used for the samples discussed here Because we use nanotubes with a small bandgap, we can test memory device properties for the same sample, with very different...
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báo cáo hóa học:" Stability of SiNX/SiNX double stack antireflection coating for single crystalline silicon solar cells" pdf
Ngày tải lên :
21/06/2014, 17:20
... [HF] solution for 30 s The drive-in process is conducted for 25 at 860°C Next, a second doping process at 810°C is followed for PSG is removed by dipping the wafer in 10% HF solution for 30 s SiNx ... source first at 830°C for PSG is removed by dipping the wafer in 10% HF solution for 30 s Then, the drive-in process is conducted at 860°C for 25 The second doping is done at 810°C for The SiNX film ... stability against ultraviolet rays First, they are exposed to the UV light for six times; 15 for the next six times; 30 for the next six times; h, five times; and then 10 h, five times Finally,...
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Báo cáo hóa học: "Research Article Diversity Techniques for Single-Carrier Packet Retransmissions over Frequency-Selective Channels" docx
Ngày tải lên :
21/06/2014, 23:20
... which is the same for all schemes For 16-QAM modulation, the MFB for the BID scheme gives the best performance, but the better performance for the CFSD scheme is due to better performance of the ... termination is performed for convolutional codes Figure shows the FER performance at the last HARQ round for separate detection and decoding, while Figure shows the corresponding FER performance for a turbo-equalizer ... corresponds to the system performance before channel decoding for coded systems Figure shows the FER performance versus the average SNR after the last HARQ round (T = 4) for QPSK and 16-QAM modulations...
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Báo cáo hóa học: " Research Article Inequalities for Single Crystal Tube Growth by Edge-Defined Film-Fed Growth Technique" ppt
Ngày tải lên :
22/06/2014, 02:20
... Young-Laplace 1.1 in its differential form This form of the 1.1 can be obtained as a necessary condition for the minimum of the free energy of the melt column For the growth of a single crystal tube of inner ... small Therefore, it is essential to obtain accurate solution for the free surface of the meniscus, the temperature, and the liquid-crystal interface position in this tinny region For single crystal ... on the pressure difference p for small and large bond numbers, in the case of the growth of single crystal rods by E.F.G technique, are summarized in According to , for the general differential...
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Báo cáo hóa học: " Research Article Interference Cancellation Schemes for Single-Carrier Block Transmission with Insufficient Cyclic Prefix" potx
Ngày tải lên :
22/06/2014, 19:20
... cancellation is also employed in order to improve the performance By substituting r(n) for Rcp H0 Tcp s(n), sfde (n) for s(n), and sub s fde (n) for ssub (n) in (34), we have ⎛ ⎡ 0(M −L)×1 ⎤⎞ ⎜ ⎥⎟ ... shortening for discrete multitone transceivers,” IEEE Transactions on Communications, vol 44, no 12, pp 1662– 1672, 1996 [6] K Hayashi, T Kojima, and H Sakai, “A new beamforming method for single ... performance than the conventional FDE, however, it still suffers from performance degradation due to the defective channel matrix Rcp H0 Tcp (= C − CISI ) In order to further improve the performance...
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Báo cáo hóa học: " Research Article Design and Implementation of an Enhanced 802.11 MAC Architecture for Single-Hop " ppt
Ngày tải lên :
22/06/2014, 22:20
... were forced to implement the 802.11 MAC standard from scratch In addition, we needed a development platform ensuring a great flexibility For these reasons, we tried to find a development platform ... PT according to the following formula: PT = − 1, SU ACL(q) N A , (1) where N A is the number of unsuccessful transmission attempts already performed by the station for the transmission of the current ... equivalent Using formulas (2a) and (2b) we compute a single sample of the slot utilization However, to avoid sharp fluctuations in the slot utilization estimates we should average these single measures...
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Báo cáo hóa học: " Research Article A Maximum Likelihood Estimation of Vocal-Tract-Related Filter Characteristics for Single Channel Speech Separation" doc
Ngày tải lên :
22/06/2014, 22:20
... techniques for SCSS are CASAbased approaches which exploit psychoacoustic clues for separation [5–13] In CASA methods, after an appropriate transform (such as the short-time Fourier transform (STFT) ... current methods are unable to separate unvoiced speech and second, the formant information is not included in the discriminative cues for separation Besides the above techniques, there have been other ... are included in the source signals as they not provide us with useful information for the separation process Generally for underdetermined BSS, a priori knowledge of source signals is used in...
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