single cell and single molecule analyses using microfluidic devices

... 293T/CD20 cell line, but not to the control 293T cell line In contrast, the lentiviral vector FUW/VSVG was able to bind to both 293T and 293T/ CD20 cell lines In addition, a quantitative virus -cell ... internalization of the viral particle Cells were then stained for the presence of viral particles on the cell surface using an anti-FM antibody and quantified using flow cytometry As shown in Fig ... transfected cells Solid line, analysis on transfected 293T/CD20 cells; shaded area, analysis on 293T cells (B) Analysis of co-expression of the FM and antibody on gated GFP-positive cells FM was...

Ngày tải lên: 12/08/2014, 04:21

... histone-fold extensions of H3 and H2B are labeled as αN and αC, respectively; the interface between the H2A docking domain and the H4 C terminus as b; and iv N- and C- terminal tail regions as ... H4; histone-fold extensions of H3 and H2B are labeled as αN and αC, respectively; the interface between the H2A docking domain and the H4 C terminus as b; and N- and Cterminal tail regions as N ... support and encouragement when I was in his group for the past years Without these, I would not have made so many achievements and it is an interesting and enriching experience for doing research and...

Ngày tải lên: 14/09/2015, 08:39

... spectrofluorimeter using a · cm pathlength cuvette, using the same conditions as in the CD experiments, with excitation at 280 nm and a bandpass of nm, and emission at 320 nm with a bandpass of nm ... spectrum (Fig 2B and Table 1) Functional analyses The functional characteristics of the chimeras were analysed by cosedimentation with actin and tropomyosin followed by SDS ⁄ PAGE, and by their ability ... and TnI regulatory domains Fig (A) TnC(1–91)–TnI(98–182) experimental data and GNOM fitting Inset: pair distance distribution function p(r) The experimental values ˚ ˚ for Dmax and Rg, 110 A and...

Ngày tải lên: 16/03/2014, 18:20

... properties of C1q toward DNA and of an emerging technique allowing the molecular combing of DNA strands and its observation by single- molecule spectroscopy Double- and single- stranded DNA has been demonstrated ... polyanionic molecules like sulfated polysaccharides and also DNA, but with the opposite effects of either inhibition or activation, respectively Using single- molecule observation of immobilized DNA strands, ... P., Li, H & Fernandez, J (2001) Fingerprinting polysaccharides with single- molecule atomic force microscopy Nat Biotechnol 19, 258–262 11 Camesano, T & Wilkinson, K (2001) Single molecule study...

Ngày tải lên: 16/03/2014, 23:20

... the rupture of A’ and G b-strands can be facilitated by the breaking of interstrand hydrogen bonds by D2O molecules These molecules form bridges between the two separating strands (Figure 5) One ... Snapshot of the bstrands A’ and G of the I27 protein showing D2O molecules bridging the protein backbone Steered molecular dynamics simulations measure the elongation of b-strands A’ and G for unfolding ... biological function, where they play a key role in cell adhesion and motility, formation and stability of proteins structures and receptor– ligand interactions.[3] To further explore the role of...

Ngày tải lên: 22/03/2014, 18:20

... for SCFDMA and OFDMA, which is a generalization of the result in [3], and it allows for the calculation of the achievable rate using arbitrary sub-carrier assignment methods in both the single user ... via a signalling channel in the downlink For simplicity, we start with the single- user single- input single- output system and then extend it to the multi-user case with multiple antennas at the BS ... different transmission bandwidths, while for SC-FDMA it decreases as the transmission bandwidth increases This may due to the fact that as the transmission bandwidth increases and when it is much...

Ngày tải lên: 21/06/2014, 23:20

... We have reviewed different methods of stretching and immobilizing DNA molecules for studying single- molecule DNA–protein interactions Single- molecule experiments overcome the problem of ensemble-averaging ... (2007) 2:185–201 base sequence information from a single- stranded template and synthesizes the complimentary strand The differences between DNAP and RNAP include (1) catalyzing of addition of a ... out using an optically trapped DNA molecule [33] Bensimon and coworkers [34] followed in real time the interaction between a type II DNA topoisomerase (topo) and dsDNA that had been stretched and...

Ngày tải lên: 22/06/2014, 18:20

... Thermal-Activated Single Molecule Diffusion 4.1.2 Laser-Activated Single Molecule Diffusion 4.1.3 Field-Induced Diffusion of Single Atoms 4.2 Single Atom and Molecule Manipulations ... of Single Molecules in Solutions 8.4.3 Single Molecule Diffusions in Living Cells 8.4.4 Single- Pair FRET 8.5 Single Molecules ... of Single Molecules at Interfaces 6.3 Intramolecular Interactions of Single Molecules 6.3.1 Elasticity of DNA Molecules 6.3.2 Folding and Refolding of Single...

Ngày tải lên: 27/06/2014, 10:20

... existing single- molecule techniques, in particular those that measure optical and mechanical signals Single- molecule fluorescence Single- fluorophore detection entails the illumination of a molecule ... of single molecules Annu Rev Biophys Biomol Struct 1997, 26:567-596 Deniz AA, Laurence TA, Dahan M, Chemla DS, Schultz PG, Weiss S: Ratiometric single- molecule studies of freely diffusing biomolecules ... structural dynamics and function in single ribozyme molecules Science 2002, 296:1473-1476 Funatsu T, Harada Y, Tokunaga M, Saito K, Yanagida T: Imaging of single fluorescent molecules and individual...

Ngày tải lên: 06/08/2014, 18:20

... ATPase and mechanical events by a single myosin molecule during interaction with actin Cell 1998, 92:161-171 Harada Y, Funatsu T, Murakami K, Nonoyama Y, Ishihama A, Yanagida T: Single- molecule ... to permit simultaneous singlemolecule fluorescence and optical trapping The choices of dyes and excitation wavelengths were optimized to minimize two-photon excitation and achieve acceptably ... several seconds, this dye photobleached in a single step and the light signal returned to background levels When suitably combined, optical trapping and singlemolecule fluorescence supply information...

Ngày tải lên: 06/08/2014, 18:20

... positioned over a single SNP, seven (11.3%) were positioned over an INDEL, two (3.2%) spanned one SNP and one INDEL, one (1.6%) spanned > SNP and no INDEL, and one spanned > SNP and > INDEL From ... et al (2004) [11] and Bonilla et al (2002) [8] are shown in (A) and (B), respectively (C) The segment of pseudomolecule map showing the physical positions of the SKC1 gene [12] and loci with SFPs ... thousands of SFP markers from a pairwise rice genotype comparison Because a number of researchers have been using Affymetrix microarrays for transcriptome analyses in a range of rice RILs, NILs and...

Ngày tải lên: 12/08/2014, 03:20

... samples were collected and analyzed Besides yield and concentrations (Fig 1A and 2A), the output rate of each parameter during the time since the Figure somatic cell of 24 (SCC) and concentration ... afternoon and morning milking Day milking interval = the time between morning and afternoon milking Data represent the LS-means SE for yield, SCC and PMN in morning and afternoon milk was 0.59 and ... Acta Veterinaria Scandinavica 2009, 51:4 http://www.actavetscand.com/content/51/1/4 Figure during the interval of fat, protein and lactose B) before and afternoon milk (column A) and the calculated...

Ngày tải lên: 12/08/2014, 18:22

... backgrounds All analyses were carried out with the complete data set and with a subset containing only genotyped females and three generations of pedigree (5,090 animals) Records were analyzed using an ... G05 and GMF despite different averages but with similar differences between average diagonal and off-diagonal elements, 0.66 and 0.68, respectively Estimates in the smaller data were similar using ... pedigree and genomic relationship coefficients Table Variance components estimates for litter size using pedigree and genomic relationship coefficients Additive Variance (ste1) Breeding values and...

Ngày tải lên: 14/08/2014, 13:21

... me the opportunity to work in Biophysics and Single Molecule Manipulation Lab and his professional guidance, mentorship and numerous help in my research and life I also would like to appreciate ... 59 iii Abstract Single molecule manipulation techniques have become versatile tools to study the force and motions of biological molecules such as DNA and proteins, and DNA-protein interaction ... strand has its own polarity One strand starts with 5’ and ends with 3’ while the other strand runs towards the opposite direction As a result, DNA double strands have opposite polarities and...

Ngày tải lên: 12/10/2015, 17:34

... bi,k+1 then n = bik + (n − bik ) and gi (n) = gi⊕bik (n − bik ), and (b) if bik < n and Bi is finite and bik is the largest member of Bi , then n = bik + (n − bik ) and gi (n) = gi⊕bik (n − bik ) ... bi.k+1 then n = bik + (n − bik ) and gi (n) = gi⊕bik (n − bik ) and (b) if bik < n and Bi is finite and bik is the largest member of Bi , then n = bik + (n − bik ) and gi (n) = gi⊕bik (n − bik ) ... bi⊕bik ,j and f (i ⊕ bik ⊕ bi⊕bik ,j , bi⊕bik ,j ) ≥ Θi (bik ) if such a bi⊕bik ,j exists Also, once bi⊕bik ,j and bi,k+1 are computed, we define Θi (bi,k+1 ) = {bi⊕bik ,j + x : ≤ x ≤ bik and f (i...

Ngày tải lên: 07/08/2014, 13:21

... Preparation of donor cells Four types of somatic cells were used as donor cells: 1) denuded cumulus cells (DCCs); 2) cultured cumulus cells (CCCs); 3) cultured follicular cells (CFCs); and 4) adult skin ... CCC-derived SCNT embryos (55 ± 6% and ± 1%, respectively) The number of blastocyst cells was not altered by the type of donor cell (28 ± and 33 ± cells/blastocyst for DCCs and CCCs, respectively) In ... pig than from SCNT using autologous follicular cells and heterologous skin fibroblasts (5 ± 2% and 10 ± 1%) There was no significant difference in blastocyst cell number (22-35 cells) among the...

Ngày tải lên: 07/08/2014, 20:23

... time was clearly shorter using RA for all patients This fact results from fewer patient positioning procedures (1 time for RA; time for every single arc using CAT) and single arc irradiation technique ... study and participated in study design HAW carried out the clinical evaluation and performed the statistical analysis DMW and HV performed physical evaluation and technical implementing HC and ... Monitor Units (MU) for CAT (Conformal Arc Therapy) in black and RA (Rapid Arc) in white The MU for each single arc using CAT were summed up and displayed For RA only one arc was used Page of led to...

Ngày tải lên: 09/08/2014, 09:20

... conceptualised and implemented the methods, performed the experiments and wrote most of the paper DL provided the datasets and supervised the work and the development of ideas All authors read and approved ... effective as Punias and Hooke Conclusions Single- molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells SMFS can analyze membrane proteins ... residue-residue contacts and membrane topology Acknowledgements We thank Daniel Mueller and his group for providing the experimental data and fruitful discussions We thank Alexander Andreopoulos for...

Ngày tải lên: 12/08/2014, 17:20

... came from SG and MDP SG, MDP, EVDC, FT, FM and IH collected data and prepared the article YVB, SB and FDS critically reviewed the paper SG and MDP finalized the text All authors read and approved ... Zamorano JL: ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure 2008: the Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2008 of the European ... Acute and critically ill peripartum cardiomyopathy and ‘bridge to’ therapeutic options: a single center experience with intra-aortic balloon pump, extra corporeal membrane oxygenation and continuous-flow...

Ngày tải lên: 14/08/2014, 07:21

... properties of these important proteins at the singlemolecule level Single- molecule manipulation using magnetic tweezers and single- molecule imaging using atomic force microscopy were utilized in ... experiments Single- molecule manipulation techniques that allows us to manipulate single DNA molecule and exert forces have enabled us to better understand the underlying molecular mechanism Single molecule ... interactions are investigated to a large extent with single- molecule manipulation methods using magnetic tweezers and single- molecule imaging using atomic force microscopy (AFM) 1.2 Literature...

Ngày tải lên: 09/09/2015, 11:26