handbook of fluorescence spectroscopy and imaging from single molecules to ensembles

... ACFs of single pixels obtained from background measurements, the autofluorescence of human Neuroblastoma (SHSY5Y) cells, and SBD-TMR labeled cells 94 Figure 5.2: 1x1 binned number of particle and ... main role of the raft in this case is to concentrate the toxin receptor, to ensure maximal binding capacity of the toxin to the cell surface [101] The interaction of tetanus and botulinum toxins ... two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectroscopy (ITIR-FCS) and Imaging Total Internal Reflection -Fluorescence Cross Correlation Spectroscopy (ITIR-FCCS)...

Ngày tải lên: 12/09/2015, 09:55

... angular position of the detector arm have to be optimized The sample is mounted into the setup and the sample motor moved to 45° while the detector arm moves to 90° according to the reflection ... state of S1 is complete prior to emission of photons and consequently fluorescence starts from the lowest vibrational level in S1 (Kasha’s rule) [26] From there the molecule can decay to different ... and the subsequent decay of the fluorophore to higher vibrational levels of S0 This shift is fundamental to the sensitivity of fluorescence techniques, because it allows the emitted photons to...

Ngày tải lên: 08/11/2015, 17:01

... publication of ASHRAE Standard 90.1-1999 and ASHRAE Standard 62-1999, often called the Energy standard and Indoor Air Quality standard, the second edition of Handbook of Air Conditioning and Refrigeration ... Director of the Research Institute of Air Conditioning in Beijing from 1963 to 1966 and from 1973 to 1974 He helped to design space diffusion for the air conditioning system in the Capital and ... the type and size of the windows and the construction of external walls and roofs Location of the ductwork and piping to avoid interference with each other, or with other trades Layout of the diffusers...

Ngày tải lên: 29/10/2013, 20:15

... resistance of a thermistor may drop from approximately 3800 to 3250 ⍀ when the sensed air temperature increases from 68 to 77°F (20 to 25°C) Recently developed high-quality thermistors are accurate, ... calculation, the enthalpy of moist air is taken to be equal to the enthalpy of a mixture of dry air and water vapor in which the amount of dry air is exactly equal to lb (0.454 kg) Based on the ... temperature of heat source of reversible engine, °R (K) TRo ϭ temperature of heat sink of reversible engine, °R (K) Two of the ASHRAE basic tables, “Thermodynamic Properties of Moist Air ” and “Thermodynamic...

Ngày tải lên: 29/10/2013, 20:15

... photo/acid etched and polished tools’ Choosing filler wires There are thousands of different types of tool steel, and many types of tool copper and tool aluminum, but only a limited number of tool ... 24 Handbook of mold, tool and die repair welding ' Table 3.2 Types of tool and their possible material type Types of tool Possible types of material Anvils Axes Blades flying shear granulator ... risk and from time to time you have to take that risk Because of customer demands I have welded hundreds of tools this way and I will continue to so, but in this situation, it is important to use...

Ngày tải lên: 05/01/2014, 19:45

... 31% of the riboflavin, 33% of the phosphorus, 19% of the protein, 16% of the magnesium, 21% of the vitamin B12 , 17% of the vitamin A, 10% of the vitamin B6, and 6% of the thiamin in addition to ... from 2.5 to 9.1 mg/g fat In cheeses, the CLA content is reported to range from 3.6 to mg/g fat; in fluid milks from 3.3 to 6.4 mg/g fat; and in butter from 5.5 to 6.5 mg/g fat Moreover, 90% of ... gland Lactose accounts for about 54% of the total solids-not-fat content of milk and contributes about 30% of the energy (calories) of whole milk Cow’s milk contains about 4.8% lactose (12 to...

Ngày tải lên: 15/02/2014, 16:20

... stimulation of various effector enzymes and formation of second messengers, leading to aggregation and secretion of granule contents Specific mechanisms involved in the process of centralization of granules ... stimulation of phospholipases and formation of second messengers that facilitate mobilization of cytosolic free calcium, centralization of granules, assembly of filamentous actin, expression of binding ... athrombin, TLP and its homologs induced less expression of the activated, bindingcompetent form of GPIIb/IIIa, failed to cause association of GPIIb and pp60gtc with the cytoskeleton, failed to sustain...

Ngày tải lên: 17/02/2014, 05:20

... nearer to a site where it can best be neutralized by protonation – the N5 position Electron transfer from PSI to flavodoxin Fd and Fld differ in size and in the chemical nature of their redox cofactors ... between the FMN N(5) and the NH of V59, in favour of a stronger H-bond between the carbonyl of N58 (‘O-up’ conformation) and FMN N(5)H [41] The semiquinone states of A nidulans and Anabaena Flds ... resonance and 1D and 2D electron spin echo envelope modulation spectroscopies applied to AnFldsq also led to assignment of the interaction parameters of N(1), N(3), H(5), H(6), CH3(8) and N(10)...

Ngày tải lên: 18/02/2014, 11:20

... the land diverted from food to fuel production, and for food prices Chapters in this handbook use economic modeling tools to provide insights into these issues The introductory part of this handbook ... carbon and on VMT The extent to which a biofuel mandate leads to displacement of gasoline and reduction in GHG emissions and VMT depends on the elasticity of supply of gasoline and the elasticity of ... biorefineries, and processing and distribution of ethanol and coproducts The model aims to minimize the total system costs for transportation and processing of feedstock, transportation of ethanol from...

Ngày tải lên: 18/02/2014, 14:20

... banana and a handful of dry cereal A cereal bar and a container of yoghurt A bagel with a light spread of peanut butter A sports gel or small packet of honey and a glass of water A glass of juice and ... internationally The editor and contributing authors of this Handbook have covered in detail all of the basic science, the clinical aspects of injuries and other health concerns, and the practical ... unusual assemblage of knowledge and skill on the part of every health professional involved This Handbook not only presents basic scientific and clinical information, but the editor and authors address...

Ngày tải lên: 21/02/2014, 17:20

... role of an ingredient in a product is needed, of its limitations, mechanism of action, and regulatory constraints Part four, the largest one, is the core of the handbook and provides guidance to ... book Details of most of the above fields are covered in the different volumes of the ‘‘Cosmetic Science and Technology’’ series In the first edition of the ‘ Handbook of Cosmetic Sciences and Technologies,’’ ... Encapsulation to Deliver Topical Actives 125 ´ Jocelia Jansen and Howard I Maibach Introduction 125 Design Aspects of a Vector 125 Properties of a Vector 131 Dermatological and...

Ngày tải lên: 03/03/2014, 11:56

... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L ... identity of the C region of LdUSP is also very high among all sequences (89–95%) Both D and E ⁄ F regions are also highly homologous to those of T molitor and L migratoria (96% and 75%, 88% and 69%, ... homologous to those of other EcRs (> 60%), especially to EcR-A from coleopteran Tenebrio molitor (TmEcR-A, 91%) and orthopteran Locusta migratoria (LmEcR, 89%) The D region is homologous to those of...

Ngày tải lên: 07/03/2014, 21:20

Ngày tải lên: 17/03/2014, 13:36

... inhibited and no cell was observed at 0.8%w/v According to Cao Xuan Thang (2004) [9] this may due to inhibition of PEG on cell and dissolve of oxygen Table 2: Effect of surfactants to the bioremediator ... milling, drying and sterile at 121oC for 15 minutes, 0.4% w/v of CF-18 and 0.2 %w/v of PVA were added to mixed starter Mixed solution was incubated and dried to 10% of moisture Table Effect of concentration ... added The increasing of cell density may due to attachment of cell to the PVA network Optimal concentration was advised as 0.2 %w/v Storage and application of bioremediator Straw was used for...

Ngày tải lên: 22/03/2014, 09:20

... Nagel 54 Handbook of Regulation and Administrative Law, edited by David H Rosenbloom and Richard D Schwartz 55 Handbook of Bureaucracy, edited by Ali Farazmand 56 Handbook of Public Sector Labor ... TRACON area and enters a sector, another hand off occurs These hand offs continue as the aircraft lies through sectors and centers eventually into another TRACON and then an airport area to land In ... Charney 122 Handbook of Technology Management in Public Administration, edited by David Greisler and Ronald J Stupak ˘ 123 Handbook of Decision Making, edited by Göktug Morçöl 124 Handbook of Public...

Ngày tải lên: 22/03/2014, 19:20

... setup of isotonic sodium chloride to one end of the stopcock, and to the other end of the stopcock attach a piece of intravenous tubing, which should be taped vertically to a pole or a piece of ... alleviation of symptoms, such as reduction of inflammation; (3) cleansing and debridement; (4) protection; (5) restoration of hydration; and (6) reduction of scaling and callus Many different forms of ... instill to drops of 0.5% proparacaine in the nostril of the cat or small dog; 0.5 to 1.0 mL of 2% lidocaine instilled into the nostril of a larger breed dog may be required to achieve the level of topical...

Ngày tải lên: 23/03/2014, 00:20

... Committee of the American Society of Appraisers He has served as president of the board of directors of the Chicago Chapter of the American Society of Appraisers and as an adjunct professor of finance ... School of Continuing and Professional Studies Mr Lee is a chartered financial analyst and received his BSE in economics from the Wharton School of Finance and Commerce of the University of Pennsylvania ... related to the topical issues in business and intangible asset valuation to be added Instead of simply updating the Handbook of Advanced Business Valuation, this text expands the scope of our...

Ngày tải lên: 30/03/2014, 10:20

... is the mean activity of the salt, and LU and LF are the number of salt ions bound by the unfolded and folded states of the apoprotein, respectively Determination of kinetic and thermodynamic constants ... conformational stabilities of the two forms of the protein at high concentrations of salt are probably due to a combination of factors including the preferential binding of salt ions to the folded protein, ... Kinetics and Mechanism, 2nd edn John Wiley, New York 26 Burstein, E.A., Vedenkina, N.S & Ivkova, M.N (1973) Fluorescence and the location of tryptophan residues in protein molecules Photochem Photobiol...

Ngày tải lên: 31/03/2014, 15:20

... examples to aid understanding Extensive current and retrospective production and consumption data has been maintained and expanded from the first edition to give an idea of the xxiii Hocking: Handbook ... company to employ chemists, engineers, biologists, and other professionals to help ensure the continuing discovery and development of new products for its success From 2.5 to 3.5% of the value of ... )n , and selected from those of Peters and Timmerhaus [15] and Allen [16] a Hocking: Handbook of Chemical Technology and Pollution Control 0120887967_chap01 1.5 CHEMICAL PROCESSING Final Proof...

Ngày tải lên: 01/04/2014, 11:46

... Handbook, and Handbook of Plastics, Elastomers, and Composites, this Handbook of Ceramics, Glasses, and Diamonds has been prepared as a thorough sourcebook of practical data for all ranges of interests ... manufacture, and fabricate materials and forms made with ceramics, glasses, and diamonds A companion to my other series handbooks, namely, Handbook of Materials for Product Design, Modern Plastics Handbook, ... referred to as toughening, can increase the strength and toughness of alumina Schwartz discusses the properties of alumina toughened by additions of various forms of zirconia, and by additions of silicon...

Ngày tải lên: 02/04/2014, 15:40