... that this new delivery system can help tissue regeneration and wound healing by releasing the growth factors in a controlled and temporal manner Base a longer release period, around weeks [13] ... internalization of MSNs, indicating that the MSNs are not only a biocompatible delivery system for growth factors/ proteins, but also an ideal biomaterial for the application in bio-imaging Conclusions ... Bioactive Materials 25:996–997 13 S.H Choi, T.G Park, Int J Pharm 311, 223 228 (2006) 14 R.H Muller, C Jacobs, Int J Pharm 237 , 151–161 (2002) 15 D.D Hile, M.L Amirpour, A Akgerman, M.V Pishko,...
... normoxia; (c) hypoxia) and presence ((b) normoxia; (d) hypoxia) of fibroblastgrowthfactor (FGF2) Scale bar, 100 µm FGF2, fibroblastgrowthfactor specific type II collagen gene promoter activity [35] ... tension (n = 3) *p < 0.05; **p < 0.001 (Student's unpaired t test) in cell aggregates from fibroblastgrowthfactor (FGF2)-expanded versus non-FGF2-expanded cells P2, passage 2; P3, passage AGCTTCTGTGGAACCATGGAA-3' ... under hypoxia after 14 days in culture AGG, aggrecan; BGN, biglycan; DCN, decorin; FGF2, fibroblastgrowthfactor 2; FMOD, fibromodulin; P2, passage 2; P3, passage Page of (page number not for citation...
... proinflammatory cytokines and growth factors such as IL-1 [5,11,12] – and potentially fibroblastgrowthfactor (FGF2) (otherwise known as basic fibroblastgrowth factor) [13] – both of which have ... Arthritis Rheum 1982, 25:1228- 1238 Posever J, Phillips FM, Pottenger LA: Effects of basic fibroblastgrowth factor, transforming growth factor- beta 1, insulin-like growth factor- 1, and insulin on human ... disc tissue An immunohistochemical analysis of transforming growthfactor beta, fibroblastgrowthfactor and platelet-derived growthfactor Eur Spine J 2006, 15:588-596 Melrose J, Smith S, Little...
... present in the IPFP tissue of fibroblastgrowth factor- 2 (FGF-2) expansion Chondrogenic cultures of infrapatellar fat pad (IPFP) cells and effects of fibroblastgrowth factor- 2 (FGF-2) expansion ... ascorbate 2-phosphate, 100 nM dexamethasone, 10 ng/mL transforming growthfactor (TGF)-β3, and 100 ng/mL insulinlike growth factor- 1 (all from Sigma-Aldrich) Gene expression analysis Quantitative ... of differentiation potential Fibroblastgrowth factor- 2 (FGF-2) is a potent mitogen for a variety of cell types derived from the mesoderm, including chondrocytes [23, 24] It has been shown to enhance...
... signaling is transduced through the formation of a complex of a growth factor, a proteoglycan, and a high-affinity fibroblastgrowthfactor receptor (FGFR), which is a transmembrane tyrosine kinase ... Arthritis Research & Therapy Vol 10 No Uchii et al Fibroblastgrowthfactor (FGF) is a family of at least 24 growthregulatory proteins – sharing 35% to 50% amino acid sequence ... osteoarthritis Ann Rheum Dis 2005, 64:694-698 Szebenyi G, Fallon JF: Fibroblastgrowth factors as multifunctional signaling factors Int Rev Cytol 1999, 185:45-106 Draper BW, Stock DW, Kimmel CB:...
... endothelial growthfactor (VEGF) and basic growthfactor (bFGF) In angiogenesis, a number of growth factors (for example Angiopoietin-1, Platelet derived growth factor, Hypoxia inducing factor- 1a, ... basic fibroblastgrowthfactor and vascular endothelial growthfactor in sera of cancer patients Br J Cancer 1997, 76 :238 -43 27 Kondo S, Asano M, Suzuki H: Significance of vascular endothelial growth ... endothelial growthfactor J Biol Chem 1994, 269:26988-95 20 Dietz A, Rudat V, Vanselow B, Conradt C, Möhler T: [Predictive value of serum levels of basic fibroblastgrowth factor, vascular endothelial growth...
... Fibroblastgrowthfactor receptor is principally responsible for fibroblastgrowthfactor 2-induced catabolic activities in human articular ... chondrocytes Matrix Biol 2001, 20 :233 -242 15 Sah RL, Trippel SB, Grodzinsky AJ: Differential effects of serum, insulin-like growth factor- I, and fibroblastgrowth factor- 2 on the maintenance of ... 44:585-594 Loeser RF, Chubinskaya S, Pacione C, Im HJ: Basic fibroblastgrowthfactor inhibits the anabolic activity of insulin-like growthfactor and osteogenic protein in adult human articular chondrocytes...
... Bovin Serum fgf FibroblastGrowthFactor Gene FGF FibroblastGrowthFactor FGFR FibroblastGrowthFactor Receptor HRP Horseradish peroxidase iFGF Intracellular FibroblastGrowthFactor IPTG Isopropyl-β-D-thio-galactoside ... -1- LỜI MỞ ĐẦU Nhân tố tăng trƣởng nguyên bào sợi FGF-2 (Fibroblast Growth Factor- 2) protein đa chức năng, tham gia điều hòa hoạt động nhiều trình thể nhƣ cân nội ... khác FHF1-FHF4 (FGF homologous factors 1-4), chúng không bám vào FGFR nhƣng tham gia vào trình hoạt hóa nội bào, nên đƣợc gọi iFGF (intracellular FGF) - FGF16 đến FGF23 protein chƣa biết rõ đặc...
... 10,9 Lƣợng FGF-2 (g/l) (T) 0 ,23 ± 0,03 Hiệu thu nhận FGF-2 (E%) 4,42 ± 0,97 Kết ghi nhận đƣợc bảng 3.3 cho thấy trình lên men chủng BL21(DE3)/pET-FGF thu đƣợc 0 ,23 ± 0,03 g protein FGF-2 dạng ... trao đổi cation Tinh chế lực heparin Độ tinh (%) Hiệu suất riêng bƣớc (%) Hiệu suất thu hồi (%) 230 ,33 6,90 100 100 138,34 78,50 60,06 60,06 106,15 97,10 76,73 46,08 - 75 - Luận văn thạc sĩ sinh...
... BL21(DE3) mang vector pET-fgf2 có khả biểu rhFGF-2 tế bào chất - Đã tiến hành lên men thu nhận đƣợc 0,23g protein rhFGF-2 từ 1L dịch lên men - Đã tinh chế đƣợc protein rhFGF-2 từ dịch lên men sắc ký...
... Tổng quan tài liệu 1.1 HỌ NHÂN TỐ TĂNG TRƢỞNG NGUYÊN BÀO SỢI FGF (FIBROBLAST GROWTH FACTOR) 1.1.1 Giới thiệu chung Nhân tố tăng trƣởng nguyên bào sợi FGF đƣợc Armelin tìm ... đầu codon CUG có trọng lƣợng phân tử cao (HMW) lần lƣợt 22; 22,5; 24; 34kDa [23] Hình 1.4 Các dạng FGF-2 tạo thành [23] Sự khác biệt cấu trúc FGF-2 có trọng lƣợng phân tử lớn với FGF-2 có trọng ... FGF3, FGF7, FGF10, FGF22; FGF4, FGF5 FGF6; FGF8, FGF17và FGF18; FGF9, FGF16 FGF20; FGF19, FGF21 FGF23 Những nhân tố FGFs không đƣợc xếp vào họ (FGF11-FGF14) có tƣơng đồng trình tự cao với họ FGF...
... 5ml, Hitrap Heparin HP 5ml (GE Healthcare) - Hệ thống lên men tự động BioTron-LiFlus GX GX-05- 1230 2 thiết bị phụ trợ nhƣ: máy nén khí, hệ thống nƣớc giải nhiệt… - Hệ thống tinh chế AKTA (GE Healthcare)...
... 25 PADERA, R F (2000) Cell signaling by fibroblastgrowthfactor 2: investigations into interactions between fibroblastgrowthfactor 2, fibroblastgrowthfactor receptor and heparin-like glycosaminoglycans ... of fibroblastgrowth factorfibroblast growthfactor receptorheparin-like glycosaminoglycan complex The National Academy of Sciences 33 WANG, D I K (2000) The effects of fibroblastgrowthfactor ... AL., (1997) Biological Roles of FibroblastGrowth Factor- 2, Endocrine Reviews, vol 18, no 1, pp 26-45 A LOGAN, A BAIRD, (1996) "Fibroblast growth factors," Growth Factors and Cytokines in Health...
... SN: Identification of the major fibroblastgrowth factors released spontaneously in inflammatory arthritis as platelet derived growthfactor and tumour necrosis factor- alpha Clin Exp Immunol 1991, ... platelet derived growthfactor B chain (PDGFB/c-sis) mrna and immunoreactive PDGF B-like polypeptide by rheumatoid synovium: coexpression with heparin binding acidic fibroblastgrowth factor- 1 J Rheumatol ... signaling and fibrogenesis in cultured fibroblasts [26,27] Hence, the reversal of the growth factor- induced synergy by imatinib indicates involvement of specific growthfactor signaling pathways With...
... as a potent inhibitor of hepatocyte growthfactor activator (HGFA), a blood coagulation factor XII-like serine protease that converts pro-hepatocyte growthfactor (pro-HGF) to the active form ... Morimoto Y & Kitamura N (1995) Activation of hepatocyte growthfactor by two homologous proteases, blood coagulation factor XIIa and hepatocyte growthfactor activator Eur J Biochem 229, 257–261 Miyazawa ... human hepatocyte growthfactor Biochem Biophys Res Commun 163, 967– 973 Brinkmann V, Foroutan H, Sachs H, Weidner KM & Birchmeier W (1995) Hepatocyte growthfactor ⁄ scatter factor induces a...
... for amphiregulin, EGF and transforming growth factor- a are viable [4] These ligands are synthesized as transmembrane proteins, and soluble ligands (growth factors) are released into the extracellular ... activation of the kinase domain of epidermal growthfactor receptor Cell 125, 1137–1149 Voldborg BR, Damstrup L, Spang-Thomsen M & Poulsen HS (1997) Epidermal growthfactor receptor (EGFR) and EGFR mutations, ... Mitsudomi T & Yatabe Y (2007) Mutations of the epidermal growthfactor receptor gene and related genes as determinants of epidermal growthfactor receptor tyrosine kinase inhibitors sensitivity...