controlling and assessing cell biomaterial interactions at the micro and nanoscale applications in tissue engineering
Synthesis and characterization of collagen terpolymer fibers for applications in tissue engineering
... extracellular matrix proteins and new tissue formation Therefore, the properties of such biomaterials are crucial in determining the success of many tissue engineering applications Tissue engineering ... challenging to produce large, vascularised tissues that can integrate with the host native tissues 2.2 Biomaterials for Tissue Engineering Biomaterials is one important component in tissue engineering ... extracellular matrix proteins and new tissue formation Hence, the properties of the scaffolding materials are crucial in determining the success of many tissue engineering applications The general...
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Stochastics global optimization methods and their applications in chemical engineering
... comparing them with the candidate solutions in the tabu list and excluding the candidate solutions that are nearer to the points in the tabu list Thus TS avoids repeated visits to the same points in ... Motivation and Scope of the Work Since the advent of non-linear optimization in late 60's, several developments and applications of global optimization can be found in engineering discipline including ... The zero of the tunneling function ’x2’ is used as the starting point for the next minimization phase and the second lowest minimum (point c) will be located The cycle of minimization phase and...
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Báo cáo hóa học: " Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level" ppt
... concentrations despite the apparent decrease in binding lifetimes (Fig 11B), suggesting that during the sliding the protein maintains registry with the DNA, and does not hop on and off, since the rate ... used in their experiments, both of which exhibit frequent pauses during the synthesis and show a decrease in the replication rate when the force exceeds pN The decrease in the replication rate ... solution on top of this, incubate the solution in the dark for 20 min, and then illuminate and image the DNA quickly, to avoid photocleavage EcoRI recognizes and cleaves DNA at the Nanoscale Res Lett...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... were incubated with indicated concentration of insulin for 10 Mean ± SE, n ¼ subjects In this and the following figures, the insulin-stimulated effect was obtained by setting the value with no insulin ... overnight incubation When the insulin-responsiveness of the cells was examined at different concentrations of insulin, we found that insulin enhanced the phosphorylation of PKB at lower concentrations ... effect of insulin in adipocytes from controls subjects and type diabetic patients after overnight incubation Cells were incubated overnight and then with the indicated concentration of insulin for...
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Báo cáo khoa học: Roles of adenine anchoring and ion pairing at the coenzyme B12-binding site in diol dehydratase catalysis pptx
... (S)-1,2-propanediol in this drawing Pink and green colors indicate the a- and b-subunits, respectively, darkening continuously from the N-terminal to the C-terminal sides (B) Residues interacting with the adenine ... phosphate group of cobalamin (Fig 1C) [18] Residues involved in coenzyme B12 binding In this article, we report the roles of adenine anchoring and ion pairing at the AdoCbl-binding site in diol ... Fate of the adenosyl group of AdoCbl in inactivation of a mutant holoenzyme during catalysis To study the fate of the adenosyl group, the upper axial ligand of AdoCbl, in the inactivation of the...
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Báo cáo khoa học: Vps4 regulates a subset of protein interactions at the multivesicular endosome doc
... MIT domain To determine whether there is any correlation between the binding sites on Vps4p and the response of the interacting proteins to ATP binding and hydrolysis, we determined the region ... [32,33] and in addition increased binding to the N-terminal interactors, including Vps2p, Vps20p and Did2p None of the N-terminal MIT domain mutations or the C-terminal b domain mutations diminished ... N-terminal and C-terminal Vps4p domains Our finding that the interaction of Bro1p with Vps4p is regulated by ATP binding alone is intriguing Ubiquitinated cargo proteins are sorted and then deubiquinated...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... 2I–L) The same sequence of cell separation was observed in the wild type line JI116 with the cell separation process starting in the centre and extending outwards towards the epidermis of the AZ and ... seed at stage 8.1 and (B) In mature pea seed at 2.1 (C) Higher magnification of the AZ development in the young pea seed in (A) (D) Higher magnification of the AZ in the mature pea seed in (B) There ... type pea seeds and the absence of the double palisade and non abscission lines carrying the def mutant allele These findings underline key regulation of the Def locus in controlling the abscission...
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Báo cáo y học: "Natural history and clinical significance of MRI-detected bone marrow lesions at the knee: a prospective study in community dwelling older adults" pot
... pain was assessed using the five sub-scales, which included knee pain while walking on a flat surface, going up and down stairs, at night while in bed, sitting or lying, and standing upright These ... TW participated in interpretation of the data, and critically revised the manuscript GZ carried out data collection, participated in interpretation of the data, and critically revised the manuscript ... account for the correlated readings within an individual and examine the association between changes in WOMAC scores (pain, function, and stiffness) and continuous changes in BML size Standard diagnostic...
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Estrogen receptor a mediated long rang chromatin interactions at the ret gene locus in breast cancer
... the dependence on ligand activated ERα, these recent data indicates a key role of ERα in the mediation of long range chromatin interactions 11 Using the ChIA-PET assay, a genome-wide chromatin ... superfamily, the ERs have three major domains, the Activation Function domain, the DNA Binding Domain and the Ligand Binding Domain (Shiau, Barstad et al 1998) After estrogen activation, the estrogen ... long range chromatin interactions around the RET 3.3.2 Long range chromatin interactions at the RET gene requires ERα 46 50 3.4 Role of other co-factors in long range chromatin interactions around...
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Customer satisfaction, return intention and word of mouth at the coffee bean tea leaf in the vietnamese market
... plays an important role in increasing sale revenue of the company and expanding market share in the industry due to maintaining of old customers (repurchasing) and wining new ones (word-of-mouth ... these Customer Satisfaction, Return Intention & Word-of-mouth international trends and more and more new standards and lifestyles have developed Instead of gathering and dining at home after work, ... research is quantitative and cross-sectional in nature Quantitative associates numbers and statistics whereas the second measures a sample at a single point in time (data are gathered on variables...
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Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... and endothelium and resulted in the formation of atherosclerosis (9) Javid et al also found that in the early stage of atherosclerosis, the number of Cx43 gap junction plaques increased and the ... inner and outer internal elastic laminas were clear and intact in the normal group The nucleus of endothelial cells was blue and arranged regularly, and the nucleus of smooth muscle cells in the ... junction, is expressed in the abdominal aortic smooth muscle cells in rabbits and up-regulated in the early proliferation of atherosclerosis The study showed that Cx43 and Cx40 protein can not form...
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Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
... results in the downregulation of several genes that are rapidly upregulated in the shade avoidance response, including the auxinrelated genes IAA19 and IAA29 [63] The negative regulator of the shade ... mutator strains with the highest rates of mutagenesis – in the range of 10–100 times that of the average mutation rate – have been found to have mutations in the mismatch repair genes mutS and ... variation is important in the adaptation of Arabidopsis to different winter climates [32] To determine whether the variation in vernalization response is an adaptation specific for each microclimate,...
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Báo cáo khoa học: ‘Big frog, small frog’ – maintaining proportions in embryonic development Delivered on 2 July 2008 at the 33 rd FEBS Congress in Athens, Greece pot
... for tissue pattern This was demonstrated most extensively in the Drosophila wing imaginal disc Scaling is maintained upon mutations in components of the insulin-signaling pathway, which alter tissue ... by the protease, and the ligands are released This process allocates the ligands and concentrates them in the ventral side such that the total BMP ligands distribution, free and in complex, is ... determined by intrinsic parameters (e.g the diffusion and degradation rates of the morphogen) in a way that is independent of embryo size How then can one account for the clear scaling of pattern...
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Báo cáo hóa học: " Some new nonlinear integral inequalities and their applications in the qualitative analysis of differential equations" potx
... article as: Zheng and Feng: Some new nonlinear integral inequalities and their applications in the qualitative analysis of differential equations Journal of Inequalities and Applications 2011 2011:20 ... blow up in finite time Then T = ∞, and the solutions of (46) are global Zheng and Feng Journal of Inequalities and Applications 2011, 2011:20 http://www.journalofinequalitiesandapplications.com/content/2011/1/20 ... Zheng and Feng Journal of Inequalities and Applications 2011, 2011:20 http://www.journalofinequalitiesandapplications.com/content/2011/1/20 Page of 15 Theorem B: Under the hypothesis of Theorem...
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Báo cáo hóa học: " Research Article New Trace Bounds for the Product of Two Matrices and Their Applications in the Algebraic Riccati Equation" potx
... 3.1 In this section, we obtain the application in the algebraic Riccati equation of our results including 3.1 Some of our results and 3.1 cannot contain each other Theorem 3.1 If 1/p and K is the ... Majorization and Its Applications, vol 143 of Mathematics in Science and Engineering, Academic Press, New York, NY, USA, 1979 17 C.-L Wang, “On development of inverses of the Cauchy and Holder inequalities,” ... Solving 3.14 for tr K yields the right-hand side of the inequality 3.2 Similarly, we can obtain the left-hand side of the inequality 3.2 14 λi Journal of Inequalities and Applications Note that...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
... at various concentrations for 24 h The cell labeling effect was evaluated by staining cells with Prussian blue and measuring the iron-loading of cells The Prussian blue staining showed that the ... that the intensity of signal in the liver region and kidneys was closely related to the dose of the intravenously injected IRDy800CW-MNPs In comparison, the intensity of the NIRF signals in the ... atmosphere, and maintained at that temperature for 30 The resulting solution was cooled and precipitated by an addition of excess ethanol and centrifugation The precipitate containing Fe O nanoparticles...
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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx
... inflammatory cytokines could reduce the activation of hepatic stellate cells Inhibition of the proliferation of T cells thereby modulating the pro-inflammatory cytokines such as TNF-a and IL1bby MSC ... Hepatic injury activates the secretion of cytokines of inflammatory cascade from the multiple inflammatory as well as parenchymal cells, which involve the healing process Suppression of inflammatory ... examined in control and treated groups by immunostaining Alb and CK18 proteins increasingly expressed in the MSC/+HGF-CNP treated group compared to other groups (Figure 2A and 2B) despite continuous...
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The syntheses of bis guanidium salts and their applications in asymmetric mukaiyama type SN2 alkylation reactions
... on the model benzylation reaction, the catalyst could only provided moderate yield and ee But there were some innovations in the catalyst design, one was the usage C3-symmetric structure rather ... than that of C2-symmetric; the other innovation was the great flexibility of the catalyst Bifunctional character of the catalyst was the key to ee induction (Scheme 1.13) 22 Chapter The Syntheses ... 1.1) The catalyst could distribute in both organic and aqueous layers, and exchanged the anions in the aqueous phase and took them back to the organic phase Without the phosphosium salt as the catalyst,...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... N-terminal Cys containing proteins at the C-terminus of the Ssp DnaB mini intein (17kDa) using the pTWIN vector (NEB, USA) Intein-mediated cleavage occurs between the last amino acid of the mini intein ... USA) and cloned into pTWIN1 vector The genes were inserted in frame after the C-terminal of the Ssp DnaB intein (Intein1) in the pTWIN1 and pTWIN2 vectors, with the first amino acid of the protein ... live cell bioimaging In the first application for live cell bioimaging, a protein of interest having an Nterminal cysteine was expressed inside a live cell using intein-mediated protein splicing...
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Hydraulic modeling of open channel flows over an arbitrary 3-d surface and its applications in amenity hydraulic engineering
... equations, usually called the 13 Saint-Vernant equations The new equations possess terms containing explicitly the curvature and the derivative of the curvature The resulting streamlines were therefore ... Kelvin’s theorem, the circulation is conserved therefore the velocity at the center is infinite If the pressures remain hydrostatic, the depression of the free surface is also infinite But the ... moves with the flow At a point on the path, one particle may have velocity components Vn and Vt normal and tangential to the path For the path to remain unbroken and assuming the flow is incompressible,...
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- the o fid and its applications in petroleum product analysis
- m3 2 1 e3 2 1 at the t matrix pole found in the framework of dispersion relations
- murmur best heard at the second right intercostal space in an 82 year old asymptomatic man
- a unique single centre experience at the scientific research pediatric centre in the russian federation
- enthalpy of noncovalent interactions at the carboxylate moieties of the ligand
- adipose derived stem cells and future applications on tissue engineering
- to study allelochemical interactions at the molecular level
- cell biomaterial interactions at the micro and nanoscale
- protein lipid interactions at the interface
- protein lipid interactions at the oil aqueous interface
- tumor host interactions at the metastatic site mkk4 signal transduction and the stress response
- rf 6a cell vegf expression at the hypoxic condition
- the potential of stem cells in tissue engineering nicholas d evans and julia m polak
- biomedical imaging and image processing in tissue engineering
- 4 experimental and clinical applications of tissue engineering in omfs
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