cloning sequence analysis and tissue expression of translationally controlled tumour protein fromthe wssv infected indian shrimp penaeus indicus
Báo cáo khoa học: Induction of translationally controlled tumor protein (TCTP) by transcriptional and post-transcriptional mechanisms pot
... structure and complete sequence of the rabbit gene coding for the translationally controlled tumor protein (TCTP) P23 Eur J Biochem 257, 62–68 Thiele H, Berger M & Thiele BJ (2000) Expression of the ... Effect of TCTP mRNA 5¢- and 3¢-UTR on luciferase reporter gene expression in the presence of TCTP-inducing substances.The complete 5¢- and 3¢-UTR of human TCTP mRNA were cloned upstream and downstream ... northern blot and rRNA staining (B) Statistical analysis of three independent experiments For mRNA quantification, the sum of both TCTP transcripts of 0.8 and 1.2 kb [22] was used Inhibition of transcription...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... phosphorylase-a and activation of glycogen synthase [28] Overexpression of PTG caused inactivation of phosphorylase (Fig 4A), activation of glycogen synthase and stimulation of glycogen synthe- ... varying titres of adenovirus for expression of PTG and cultured for 18 h (A) Hepatocytes were incubated for determination of glycogen synthesis and the activities of phosphorylase and glycogen ... phosphorylase-a and glycogen synthesis Using three independent methods involving either expression of the muscle isoform of glycogen phosphorylase, or expression of the glycogen-targeting protein PTG...
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Báo cáo y học: "Several clinical interests regarding lung volume reduction surgery for severe emphysema: meta-analysis and systematic review of randomized controlled trials." docx
... meta -analysis and systematic review of randomized controlled trials (RCTs) Methods: Eight RCTs published from 1999 to 2010 were identified and synthesized to compare the efficacy and safety of ... conflicts of interest that are directly related to the content of the study Authors' contributions: BD and WH carried out to study design and RCTs assessment WH, RWW and BD carried out data analysis and ... the 6th month and 12th month were improved significantly than of group B, despite no difference on the 3rd month Quality of life (QOL) of group A was better than of group B in and 12 months VATS...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... parotid gland, the uterus, the seminal vesicle, the thyroid and parathyroid glands and the Fig Immunohistochemical analysis of the A and B antigens expression in BDIX rat tissues Frozen BDIX rat tissues ... Availability of the rat A gene sequence will allow the search of genetic polymorphisms at the Abo locus in this species Comparisons of the tissue expression across species, as well as of the sequences and ... cells of the neck area and on the parietal cells of the gastric glands whereas the B antigen was present on the pits epithelial cells and the chief cells of the glands In the large intestine and...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... cycles of 94 °C for s and 72 °C for min; five cycles of 94 °C for s and 70 °C for min; and 20 cycles of 94 °C for s and 68 °C for The RACE–PCR product was then cloned into the PCR-ScriptTM Amp Cloning ... rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression levels of the ... transcript is abundant in human brain and skeletal muscle (Fig 2A), and present at a very low level Fig Northern blot analysis of the gene expression of human b1B (A,B) and b1 (C,D) subunits, using human...
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Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
... elements of rmGP [4] and EcMalP [3] onto the linear sequence of StP and thus measures the extent of conservation of the respective ÔsitesÕ in StP As in other GPs, the catalytic site and the PLP ... structural analysis of phosphate and sulphate binding sites in proteins Estimation of propensities for binding and conservation of phosphate binding sites J Mol Biol 242, 321–329 22 Nandi, C.L., ... incorporation of cofactor during folding of the recombinant proteins had taken place correctly Circular dichroism (Fig 3) and Fourier-transform infrared spectra (not shown) of the purified recombinant proteins...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... BmKAEP and BmKIT2), but Arg6 or Lys6 and Thr57 are conserved in other scorpion species Cloning and analysis of genomic sequence of BmKIM We have isolated, cloned and sequenced the genomic regions encoding ... Construction of expression vectors and expression of the fusion proteins The cDNA encoding BmKIM was amplified by PCR with forward and reverse primers The noncoding regions, the signal peptide and the ... cleavage of fusion protein and purification of the recombinant BmKIM The fusion protein was cleaved completely with an enterokinase/substrate ratio of 50% at 26 °C for 10 on the Fig Expression and...
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báo cáo khoa học: " Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca)" pot
... Figure Alignment of predicted MYB domain protein sequences from spruce and pine Alignment of predicted MYB domain protein sequences from spruce and pine Amino acid sequence alignments of the 21 conifer ... secondary xylem and are also upregulated during the formation of compression wood Results Isolation and sequence analysis of 18 R2R3-MYB genes from spruce and pine We isolated and sequenced 18 full-length ... sequences were assigned to of the 22 subgroups previously defined based upon Arabidopsis sequences [18] Four of the conifer sequences (PgMYB2 and 4; PtMYB2 and 4) clustered with Arabidopsis sequences...
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Expression analysis and functional significance of chondroitin sulphate proteoglycans and heparan sulphate proteoglycans in prostrate cancer
... Materials and Methods 35 2.1 Genome-wide expression profiling of prostate 35 adenocarcinoma tissues 2.1.1 Collection of prostate adenocarcinoma tissues 35 2.1.2 Haematoxylin and eosin staining of prostate ... cytometry 59 2.3.15 Microarray analysis 60 Chapter Expression Profiling of Prostate Adenocarcinoma Tissues 62 3.1 Selection of tissues to be used for study 62 3.2 RNA quality of tissue samples used for ... prostatic and adenocarcinoma tissues 4.1.2.3 Comparison of non-sulphated chondroitin expression 117 in different grades of prostate adenocarcinoma tissues 4.1.3 Expression analysis of chondroitin-4-sulphate...
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EXPRESSION ANALYSIS AND FUNCTIONAL STUDY OF HS3ST3B1 IN HUMAN PROSTATE CANCER
... Statistical analysis 50 Chapter Results 51 3.1 Expression and functional analysis of HS3ST3B1 in 51 prostate cancer 3.1.1 Expression of HS3ST3B1 in prostate cell lines 51 and tissues 3.2 Functional analysis ... Extraction of protein 41 2.1.11.2 Preparation of resolving gel 41 2.1.11.3 Preparation of stacking gel 42 2.1.11.4 Separation and eventual visualization of 42 proteins 2.1.11.5 Densitometric analysis of ... to offer his support and advice I would also like to thank Professor Bay Boon Huat and Associate Professor Tay Sam Wah, Samuel for their timely encouragement and advice Professor Bay and Professor...
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Rate-Distortion Analysis and Traffic Modelling of Scalable Video Coders
... ACF of {εI (n)} and that of {φI (n)} in Star Wars IV (b) The ACF of {εP (n)} and that of {φP (n)} in The Silence of 1 the Lambs 125 63 The ACF of {A3 (ε)} and ... mean sizes of P and B-frames of each GOP given the size of the corresponding I-frame in (a) the single-layer Star Wars IV and (b) the base layer of the spatially scalable The Silence of the Lambs ... gratitude and respect first go to my advisors Prof Andrew Chan and Prof Dmitri Loguinov This work would never have been done without their support and guidance I would like to thank my co-advisor Prof...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... subtypes of TRHR cDNA (xTRHR1, xTRHR2 and xTRHR3) from Xenopus brain These encode the entire sequences of the proteins The amino acid sequence of xTRHR1 is very similar (74–78% identity) to that of ... bladder and stomach The xTRHR subtypes were identified by purifying the 550-bp PCR product from all the Xenopus tissues, cloning them in the pGEM-T easy vector, and sequencing Sequence analysis of ... tail of xTRHR3 (Cys342) The complete nucleotide sequences of xTRHR2 and xTRHR1 were obtained with the same strategy as that used for xTRHR3 The nucleotide sequences of the translated region of...
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Tài liệu RATE-DISTORTION ANALYSIS AND TRAFFIC MODELING OF SCALABLE VIDEO CODERS ppt
... ACF of {εI (n)} and that of {φI (n)} in Star Wars IV (b) The ACF of {εP (n)} and that of {φP (n)} in The Silence of 1 the Lambs 125 63 The ACF of {A3 (ε)} and ... mean sizes of P and B-frames of each GOP given the size of the corresponding I-frame in (a) the single-layer Star Wars IV and (b) the base layer of the spatially scalable The Silence of the Lambs ... gratitude and respect first go to my advisors Prof Andrew Chan and Prof Dmitri Loguinov This work would never have been done without their support and guidance I would like to thank my co-advisor Prof...
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Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf
... splicing A variety of tools are available to us for this purpose and further investigation of these sequence variants will not only further our understanding of the splicing mechanism and improve clinical ... for the weak donor site [9] Translationally silent variations that affect splicing and evolution With increasing understanding of the importance of cis regulatory sequences located either in ... (mouse–rat and human–dog) [10] This analysis showed a strong purifying selection at silent sites in a critical region of this gene [10], spanning the 3¢ end of exon 10 and the 5¢ end of exon 11...
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NATIONAL REPORT OF MALAYSIA ON THE FORMULATION OF A TRANSBOUNDARY DIAGNOSTIC ANALYSIS AND PRELIMINARY FRAMEWORK OF A STRATEGIC ACTION PROGRAMME FOR THE BAY OF BENGAL potx
... existing information on the use of, and threats to, the Malaysian coastal and marine resources off the Straits of Malacca and the adjacent waters of the Andaman Sea and the Indian Ocean In the process, ... and sand, caused widespread degradation of areas with mineral deposits leaving behind sandy, barren and unfertile land, unattended water bodies, and silted waterways Landslips, particularly of ... Malaysia destruction of rainforests and water catchments, and the subsequent erosion of soils together with the heavily silted run-offs, pollutes the rivers These and other sources of land-based pollution...
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Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx
... Gene expression pattern Sequence analysis Sequence analysis of the deduced protein showed that this protein has one of the longest stem region (around 200 amino acid residues) whereas the size of ... marker bands are indicated on the left side of the blot Sizes of the detected mRNA are indicated on the right (B) Expression array analysis of the expression of hST6Gal II in various human tissues ... Fig Nucleotide and predicted amino acid sequence (A) and hydropathy profile (B) of human ST6Gal II, and (C) comparison of the sialylmotifs L, S and VS of hST6Gal II with those of previously cloned...
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A meta analysis and risk assessment of heavy metal uptake in common garden vegestable
... hyperkeratosis and the formation of hyperkeratotic warts and corns on the palms and the soles of the feet are caused by chronic arsenic ingestion Discoloration of the skin of the face, neck, and back ... assessment of risk to human health, identification of potential exposure pathways, the characteristics of the affected community, the short and longterm health effects for each chemical, and analysis of ... days, and carrot-twice every days Based on the frequency of ingestion of lettuce and the relative infrequent consumption of radish, an ingestion frequency of once per week was assumed and considered...
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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot
... the analysis of the calculated structures revealed a short distances between NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 residue and C¼O of Leu5 ... [38,39] Analysis of hydrogen bonds and dihedral angles (F, C) for the average structures generated from members of cis and trans family of cyclo128 [D -Leu5]OP showed that the residues of the cis and ... analogs of OP and to use the N- and C-terminus for cyclization in order to keep the side chains unmodified We have taken advantage of the presence of an aspartic acid residue in the core sequence of...
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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt
... amount of transcripts Analysis of the amount of luciferase mRNA of transfected cells by semiquantitative RT-PCR resulted in an approximately 60% reduction of transcripts Therefore, reduced expression ... appearance of a peak pair with retention times of 8.3 and 9.5 min, and (b) the presence and (c) the correct proportion of the three major mass transitions of CEdGA,B that were used for MS ⁄ MS detection ... thorough removal of RNA from the samples and the use of negative ionization for Fig Restriction digests of CEdG-modified and unmodified pGL3 Control vector For the introduction of CEdG adducts,...
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Báo cáo khoa học: Identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum) ppt
... whereas expression of WFYT and WF2 was readily detectable in premetamorphic larvae and juveniles Tissue- specific gene expression of pleurocidin genes The results of RT-PCR expression assays of each ... locations of the first introns and exons of Y1, Y2 and Y3 An alignment of the peptides predicted from the pleurocidin genes and pseudogenes is shown in Fig All of the genes, and even most of the ... brain, muscle and skin (20–50 mg tissue) of an adult winter flounder, and from pooled samples of 20 whole larvae (hatch and d.p.h.), 10 whole larvae (15, 20, 25 30 and 36 d.p.h.) and five whole...
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