... could mean that Gremlin and Follistatin perform distinct functions in the mediating BMP responses or they are involved in different stages of signaling This point of view is supported by studies ... kinase (ALK)-3 or BMP-RIA, transcription factors Id proteins to 4, and a binding protein Gremlin, indicating that this could be a primary route for OP-1 signaling We also found that another binding ... compression In these studies, OP-1 injections reduced hyperalgesia and inhibited elevation of IL-1, TNF-a, substance P, bradykinin and their receptors in various disc tissues including spinal cord...
... routine clinical cancer diagnosis We examined the characteristics of four histopathological subtypes in lung cancer cell lines using both statistical analysis and biological network analysis In ... when examining clinical tissues with DNA microarrays or qPCR First, tissues Page of 12 contain varying amounts of contamination from neighboring stromal cells Second, RNA amplification is required ... amount of clinical tissue is limited, for instance when samples are obtained by microdissection of cancer cells While these issues are not problematic for analyzing lung cancer cell lines, they...
... phosphokinase C pathways [10], leading to a massive production of proinflammatory cytokines including interleukin-2 and interferon-g [11], resulting in extensive proliferation of T cells bearing ... respectively, using preparative isoelectric focusing as described previously [17-19] Other toxins used in this study were produced in recombinant form using SE genes cloned in this study as follows ... consisting of a single gene, as well as G3PDH and Cb genes, showed single peak A representative result obtained for Vb1 is shown in Figure 1C The melting curve for the Vb1 reaction contained a single...
... percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined by QPCR ... seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though the expression pattern of reference genes in HELA and HEP2 cells infected ... reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other HIV infections...
... ranked actin at the last position, indicating that it is an unsuitable reference gene in virus infected cells The actin gene shows significant variations with increasing degree of infection The ... highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the highest expressional stability for of viruses To find a general ... 11.28), confirming to be the most inappropriate reference gene It is remarkable that the obtained BestKeeper index values are low, despite the inclusion of Act in the calculation Calculating BestKeeper...
... percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined by QPCR ... seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though the expression pattern of reference genes in HELA and HEP2 cells infected ... reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other HIV infections...
... ranked actin at the last position, indicating that it is an unsuitable reference gene in virus infected cells The actin gene shows significant variations with increasing degree of infection The ... highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the highest expressional stability for of viruses To find a general ... 11.28), confirming to be the most inappropriate reference gene It is remarkable that the obtained BestKeeper index values are low, despite the inclusion of Act in the calculation Calculating BestKeeper...
... asexually through seeds by apomixis [3], which is classified as a pseudogamous aposporic type [4-9] Apomixis is present in more than 300 angiosperm species [10] and is being investigated by many groups ... pathways involved in both modes of reproduction The identification of genes involved in apomictic development will open the possibility of controlling the expression of this trait and engineering ... System (BioRadđ) using SYBRđ Green to detect dsDNA synthesis Reactions were done in 20 L volumes containing PCR Buffer (Invitrogen), 1.5 mM MgCl2, 0.1 mM dNTPs, 0.25 U Taq Platinum (Invitrogen), 0.1ì...
... normalization or routinely used as controls for northern blots or RT-PCR From the original list we developed a short list of nine, including genes encoding Actin-11 (ACT), Cyclophilin-2 (CYP) [10], ... Petunia lines (Figure 3) The range of values was consistently narrower in Mitchell than in V30 This could indicate that gene expression in general is less variable in Mitchell than in V30, however ... cell division and expansion in petals, or other flower tissues during this developmental stage, might explain the increased noise An alternative nonexclusive explanation is that the intermediate...
... organs in a short time by intramuscular injection of DNA vaccine [51,52] In this study, AHV-1 gC can be detected in all analyzed tissues at hour postinoculation, and the concentration of gC maintained ... been investigated in AHV-1 Cha strain-infected ducks by Qi [50] This assay was similar with Qi’s report about the distribution of the different kinds of tissues AHV-1 attenuated vaccine can be distributed ... pcDNA3.1-gC DNA vaccine We are currently studying the dynamic distribution of gC in AHV-1-infected and DNA vaccine-inoculated ducks by using this method We believe that this approach could expedite...
... Viremia peaka Outcome 30 9,130 Discharged from ICU 141 63,400 Died in ICU 133 130,000 Discharged from ICU 105 137,000 Died in ICU ICU stay (days) Viremia peak is expressed in copies/ml DBSA, deep burn ... http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics Patient Age (years) TBSA (%) 80 76 a DBSA (%)...
... presence in consumable parts of DNA vaccinated livestock, mainly in muscles Nowadays, there are no definitive guidelines available to approve usage of DNA vaccines in food- producing animals In this ... entrapped into liposomes; this compound was synthetised at the Institute of Organic Chemistry and Biochemistry, Prague), complex pDNAX:CDAN/DOPE) was administered by i.m single needle injection into ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-lissamine rhodamine B (PE-rd)(1 mol % of total lipids) Lipids used in this study were purchased from Avanti Polar Lipids, Inc., USA The lipid mixture was dissolved in freshly distilled...
... Lin School of Medicine, National University of Singapore, Singapore, Singapore ROBERTO BIASSONI • Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Istituto Giannina Gaslini, ... Medicina Molecolare Dipartimento Medicina Traslazionale Istituto Giannina Gaslini Genova, Italy Alessandro Raso Laboratorio U.O.C Neurochirurgia Istituto Giannina Gaslini Genova, Italy ISSN 1064-3745 ... (RT)-qPCR in publications associating measles virus with novel gut pathology and autism [2] A public dissection of published data at the Washington DC autism trial in 1997 revealed a catalogue of inconsistencies,...
... very small synovial tissue specimens that can potentially be used for small proof-of-concept clinical trials R358 Biomarker measurement in arthritis patients originally relied on protein analysis ... various proteins can be evaluated using IHC in specific regions such as the synovial lining, lymphoid aggregates, blood vessels and the sublining [16–18] While providing excellent spatial resolution, ... (Q-PCR) on very small synovial tissue samples using a novel cell-based standard curve technique This method is ideally suited for small proof-of-concept clinical trials designed to determine a biomarker...
... Cytokeratine (theo thứ tự protein axit bazo), sợi trung gian nhóm III gồm protein axit dạng sợi desmin, vimentin glial Loại IV bao gồm sợi protein thần kinh (NF-L, NF-M NF-H) internexin, protein nhóm ... nhóm V gọi lamin nhân (chỉ cở nhân tế bào) Các protein cytokeratin lại xếp vào nhóm VI, gồm có felensin phakinin Cytokeratin biểu mô (sợi trung gian nhóm I II) hình thành trình phát sinh có liên ... nguyên phân tử protein có khối lượng phân tử lớn (kể protein cộng hợp glycoprotein, lipoprotein nucleoprotein) polysaccharide (kể lipopolysaccharide) Các kháng nguyên protein polysaccharide tìm...
... để biết tình trạng huyết học thực trẻ sinh từ bà mẹ HIV(+) Nếu trẻ không phát sớm điều trị thuốc kháng virus Trẻ có nguy tử vong 50% 02 năm sống Kỹ thuật kinh điển chẩn đóan nhiễm HIV trẻ -RT PCR/b-DNA ... thấp trẻ sinh từ mẹ nhiễm HIV Agence Nationale de recherches sur le Sida et les hépatites virales Kỹ thuật « Real-time PCR » có độ nhạy, đặc hiệu cao, giá thành thấp so với kỹ thuật kinh điển, ... trẻ sinh từ mẹ nhiễm HIV Agence Nationale de recherches sur le Sida et les hépatites virales Tiếp cận kỹ thuật • Phương pháp: • So sánh phương pháp chẩn đoán HIV trẻ em • • Phương pháp kinh điển...
... 240 nm (theo Graindorge & Flegel, 1999)) (Nguồn: Bùi Quang Tề, 2003) 2.3.1.3 Đặc tính sinh học Virus đốm trắng ký sinh tổ chức ngoại bì, trung bì, mang, quan lymphoid biểu bì vỏ kitin Virus xâm ... tơm sú (P mondon); tơm thẻ (P indicus); tơm rảo (Metapenaeus ensis); tơm đất (M lysianassa); tơm chân trắng (L vannamei) nhập vào ni Việt Nam (Tạp chí thơng tin KHCN Kinh Tế Thủy Sản, số – 2004) ... khoảng cách 100 bp Bio - Rad Bộ ly trích Instagene Matrix cơng ty Bio – Rad: gồm có 20 ml dung dịch Instagene Matrix có hạt nhỏ lơ lửng tích điện âm protein kinase Các hạt nhỏ gắn kết với ion hóa...
... Minh City, VietNam Aug 2005 ESTABLISHING A PROCESS OF GMOs (Genetically Modified Organisms) QUANTIFICATION BY REAL-TIME PCR METHOD Instructors: Le Dinh Don and Nguyen Thai Thuy Recently, GMO is ... quantification in order to determine the percentage ratio of GMO presentation in food and feed need to be developed So, in this thesis, a process for GMO quantification will be researched and established ... Nâng cao giá trị dinh dưỡng : Mục tiêu chuyển nạp gene mã hóa protein có chứa hàm lượng cao amino acid thiết yếu: lysine, tyrosine, tryptophan vào trồng giúp nâng cao giá trị dinh dưỡng chúng [17]...
... dùng INF để điều trị VGB mạn [6] Thuốc tăng cường miễn dịch không INF Tăng cường tế bào miễn dịch chống nhiễm tế bào T sản xuất INF tự nhiên thể Bao gồm: Theradigm, Thymosin α- 1 (Zadaxin), vacxin ... triển điều trị VGB gồm: Interferon (INF), chất tương tự nucleotide, thuốc kháng virus không nucleotide, thuốc tăng cường miễn dịch không INF Interferon Interferon họ protein tự nhiên, có hoạt tính ... HIV-2, chứng minh có hoạt tính in vitro chống lại đột biến HBV kháng LAM hoạt tính in vitro in vivo chống lại HBV nguyên sinh [6] Trong thử nghiệm lâm sàng giai đoạn II, ADV chứng minh làm giảm...
... - 75% Guanin+ Cytocine Với gen > 240.000bp, có tiềm mã hoá protein cho 200 khung đọc mở (opening reading frames) có khả tổng hợp khoảng 100 protein virus Hiện nay, xác định vài protein từ gen ... thành, xin cảm ơn: Quý thầy, cô Khoa Sinh Trường Đại học Sư Phạm Thành phố Hồ Chí Minh cung cấp cho kiến thức quý báu suốt khóa học Cô TS Trần Thanh Thủy, trưởng môn Vi sinh vật, khoa Sinh, trường ... (envelope) (hình 1.6) - Là lớp lipit kép gồm lipoprotein có 33 protein cấu trúc, vài protein cấu trúc glycoprotein (glycosylate) - Những phân tử glycoprotein nằm bề mặt có phần lồi Phần lồi giúp cho virus...