... proteins in two-hybrid experiments On the other hand, the use of antibodies against AFP inin vitro and in vivo experiments investigating hexamerin uptake by the anterior fat body may give insights into ... further experiments in two distinct areas On the one hand, the binding domains of AFP and the hexamerin receptor could be mapped in detail by functional dissection using truncated prey proteins ... because of the interaction with AFP, most of the hexamerin receptor is inactivated inthe anterior fat body preventing uptake of storage protein in this part of the tissue This study opens the way...
... simulation of wind velocity inthe Hon Dau station for the two storms [11] Therefore, this model is used to calculate the meteorology field which is input for the wave model and wave setup in storm ... deepwater waves impinges almost directly on the coastline The surf zones during these conditions would vary with wave parameters but would be several hundreds of meters wide These formulas are ... However, in these studies, authors did not considered all the effects in breaking wave zone due to using wave model for large area (WAM) Another approach, Shibaki et al (2001) showed that, by adding...
... r theories: Inthe lexical entries of the wordforms there is a pointer to the rules w h i c h can be a p p l i e d withthe c o n c e r n e d w o r d f o r m as top node Our approach goes farther ... (3) the subject is obligatory, but in (2) only the direct object Inthe other cases the prepositional objects are obligatory, thus the d i s t i n c t i o n of the d i f f e r e n t meanings ... on his opinion.) (5) Die Wand besteht aus Steinen (The wall consists of stones 316 As we have seen inthe first paragraph the bundles (i.e the logical formulas) have their place inthe lexicon...
... differences are the protease-binding loop and the N-terminal part of the first b strand The alterations furthest from the binding site, in segment Gly7–Lys10, are reflected inthechangesinthe chemical ... unobservable inthe HSQC spectra compared withthe initial state The linewidths of the peaks arising from the complex were greater than those of the uncomplexed inhibitor (linewidths for the complex ... and the corresponding restraint is violated inthe complex structure lending support for the relevance of this conformational change Other violated restraints indicate changesinthe protease-binding...
... associate withthe Shb–PTB-Pro– GST fusion protein, containing both the PTB domain and two of the N-terminal proline-rich domains This interaction was only slightly inhibited by phosphotyrosine To ... phosphorylation of these proteins compared to the total amount of SLP-76 inthe CD3 treated lanes The 75-kDa protein comigrated exactly with SLP-76 These results indicate that both the SH2 domain and the PTBTyr ... interaction and the binding could be blocked out with phosphotyrosine (Fig 4A,B) To identify a putative binding site for Shb inthe Vav protein, we searched the Vav sequence for the PTB domain...
... replication of the leading strand was thought to begin first, producing an expanding displacement (D)-loop prior to the initiation of lagging strand synthesis Instead, synthesis of leading and lagging strands ... and protein with 900 mM NaCl releases a fraction of mtDNA replicative proteins and DNA binding proteins from the mtDNA These proteins (now soluble) are recovered inthe supernatant following centrifugation ... [7] The two other domains, the N-terminal and central domains, contain the ATPase activity and active site tyrosine residue, respectively These latter motifs are highly conserved among all of the...
... significantly decreased inthe ASU-treated group compared withthe placebo-treated group, withthe exception of the Safranin-O staining and the pannus on the plateaus (Table 1) Available online http://arthritis-research.com/content/11/2/R41 ... the abnormal biomechanical forces present in OA Inthe present study, treatment with ASU reduced the level of MMP-13 synthesis inthe deep zone of cartilage These findings are in accordance with ... staining positive in each zone for the specific antigen were determined The final results were expressed as the percentage of chondrocytes staining positive for the antigen (cell score), with the...
... simulation of wind velocity inthe Hon Dau station for the two storms [11] Therefore, this model is used to calculate the meteorology field which is input for the wave model and wave setup in storm ... deepwater waves impinges almost directly on the coastline The surf zones during these conditions would vary with wave parameters but would be several hundreds of meters wide These formulas are ... However, in these studies, authors did not considered all the effects in breaking wave zone due to using wave model for large area (WAM) Another approach, Shibaki et al (2001) showed that, by adding...
... treatment with glucocorticoids and use of a continuous infusion could account for the positive findings in their trial compared with previous ones and that further investigation is warranted Competing ... online http://ccforum.com/content/9/3/238 compared with placebo (P = 0.004) However, when these groups were compared individually with placebo, only inthethe group receiving 160 µg/kg was the ... placebo group (P = 0.12) Inthe placebo group these were all venous in origin and not serious, whereas inthe rVIIa group most were arterial Of these the most common were cerebral infarction, of which...
... enhanced protein catabolism in these patients Other authors have proposed alternative hypotheses to explain how the 'missing' ions inthe elevated anion gap may be generated These include the choice ... group were seen inthe patients within the lactic acidosis and 'unexplained acidosis' groups In these two groups, the concentrations of most of the acids studied were present inthe plasma ultrafiltrate ... previously thought inthe generation of the anion gap in 'classic' lactic acidosis In addition, these anions appear to have a significant role inthe generation of the anion gap in patients with acidosis...
... guidance into the IJV A guidewire was then placed through the needle into the vein, and the needle was removed A catheter or sheath was placed over the wire and advanced into the IJV (Figure 2) The ... result in a lower incidence of CVC-BSI compared withthe landmark technique ods inthe intensive care unit setting, and helped to draft the manuscript GK performed both methods inthe intensive ... haemodialysis in critical care patients The safe puncture of the IJV is achieved by using anatomical landmarks on the skin's surface and thus passing the needle along the anticipated line of the vein Many...
... of the cardinal symptoms of PTSD Examining these processes in critically ill patients will require advances in brain imaging or non-invasive measures of brain injury or inflammation embedded in ... in longitudinal studies with high follow-up rates; in our opinion, that is where medical PTSD research needs to go Competing interests The authors declare that they have no competing interests ... We predict that they are inthe brain [12] Exciting advances in neuroscience are linking fundamental psychological processes of likely relevance to PTSD such as fear, arousal, extinction, and reward...
... endangering themselves To date, even within the modern Indian subcontinent – the closest place to „home‟, the Sindhis remain somewhat a displaced people This is despite India being „home‟ to the ... ages range from the early 50s to early 70s The sons of these merchants, now in their 20s and 30s, form the third generation within the life cycle of the firm Then there is the intermediate group ... „diasporas‟ In line with my personal knowledge of „Sindhi‟, the term in all its forms (i.e „Sindh‟, „Sindhwork‟ etc.), purposefully ends with an „h‟ unlike the prevalent spelling in existing literature...
... reductases and an amino-terminal oxygenase domain containing a haem prosthetic group, which are linked roughly inthe middle of the protein by a calmodulin-binding domain 10 Figure 1.2 The general structure ... roles in inflammation, the functions of H2S in inflammation, either pro- or anti-inflammatory, are controversial Whether neutrophil infiltration is involved inthe effect of H2S inthe inflammation ... distinct roles inthe biological system eNOS eNOS which is originally characterized in large conduit vessel endothelium is now known to be expressed within the heart, inthe endothelium in both the...
... damaged protein occuring with aging We found chemically modified proteins even inthe mitochondrial matrix of adult rats, the concentration of which increased significantly with aging On the one hand ... matrix We identified the major proteins inthe mitochondrial matrix by performing SDS/PAGE separation and staining the gel with Coomassie blue or Western blotting Analysis of the SDS/PAGE revealed ... impairment inthe inner membrane The decrease in enzymatic Lon protease activity may be the result of either a loss of efficiency in relation to damaged protein and/or a decrease inthe amount...
... the coordinating K100, withthe H-bonded water molecule found inthe X-ray structure increasing the electron donating power of the coordinating amino group which in turn can decrease the reduction ... side chain slithers along the side of the protein surface, whereas inthe M100K structure it points out into the solvent (Fig 4C) The position of the K99 side chain is also different Inthe wt ... structural changes being minimal the trapping of two main-chain conformations and increased B-factors at 295 K suggests that the presence of the coordinating Lys in some way can in uence the dynamics...
... attended training sessions on the specific themes that were to be covered inthe computer assisted interviews During the interviews, the respondents were handed an information leaflet, an informed ... equal setting, while 416 took part inthe health status examination at home or in an institution Overall, 84% participated either inthe health status examination proper or inthe examination at ... was measured using two instruments, the 15D and the GHQ-12 The 15D instrument measures 15 dimensions including mobility, vision, hearing, breathing, sleeping, eating, speech, elimination, usual...
... of infection is dependent on the host immune response to the virus [20] Important changes are occurring which are impacting on the natural history of HBV infection These changes include vaccination ... is endemic inThe Gambia with 15–20% unvaccinated Gambians chronically infected [13] Liver cancer remains the commonest cause of death in adult males inThe Gambia [14] Studies of HBV infection ... sampling of HBVinfected persons with lengthy longitudinal follow-up, similar to the current study but with disease end points will allow investigators to fill inthe gaps in our knowledge of the time...
... repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix J Mol Biol 2006, 356:72-85 Kirik V, Bäumlein H: A novel leaf-specific myb-related protein with a single binding ... corresponds to the telomeric repeat-binding protein (TRP1), which also contains the typical MYB motifs [26] Eight out of these nine genes were also included inthe response to auxin stimulus, being At1g71030 ... computing the log2 of the ratio between the gene expression level in TEV-infected plants and control plants (mock inoculated) and normalized by the number of genes inthe set To minimize the number...
... fast variables, all the information needed to determine the criticality of the bifurcation is contained inthe fast subsystem but in other cases there is crucial information inthe slow dynamics ... bifurcation inthe full system will persist inthe singular limit as a Hopf bifurcation inthe layer problem It might be tempting to proceed by determining the nature of the Hopf bifurcation inthe layer ... fast compared with other processes In these cases, a classic first step is to set the fast gating variables to their quasi-steady-state values, and thereby reduce the dimension of the model by the...