Bài viết xác định điều kiện thủy phân tối ưu sụn ức gà bằng enzyme Alcalase 2.4L để hiệu suất thi nhận Glycosaminoglycans sulphate đạt cao nhất.
117 (2017) 086-092 Nguy n Th Ng c Bình*, Tr nh Th Th a Ân, Nguy n Uy Th , ng Th i h c Bách k i h c Qu c gia Thành ph H Chí Minh n Tịa so n: 20-6-2016; ch p nh -02-2017 Tóm t t Glycosaminoglycans sulphate (GAGs) s Chondroitin sulfate (CS) Glycosaminoglycans sulphate sulphate K o 4%v/wpro ian = 212 phút 27,7 Glycosaminoglycans sulfate 53,61%; Glycosaminoglycans sulfate Glycosaminoglycans sulfate Chondroitin sulfate Abstract Glycosaminoglycans sulphate (GAGs) belonging to a family of proteoglycans are complex natural polysacchrides in animals Chondroitin sulfate (CS) is a major class of Glycosaminoglycans sulphate used for treatment of arthritis, especial osteoarthritis Glycosaminoglycans sulphate can be extracted from cartilage of as cows, pigs, chicken, sharks, etc The extraction of Glycosaminoglycans sulphate from agricultural waste sources for food supplements is meaningful for Vietnam to substitute the exported material Our research is aimed to determine the optimal condition of hydrolysis of chicken keel cartilage by Alcalase 2.4 L to obtain Glycosaminoglycans sulphate The optimal values for the hydrolysis process are pH at 7,0; temperature at 540C; the ratio of enzyme and substances at %v/wpro and hydrolysis time of 212 minutes Meanwhile, Glycosaminoglycans sulphate sulfate content are obtained according to the predictions of the model at 27,78% (dry weight of raw material) Glycosaminoglycans sulphate powder product was found to be Glycosaminoglycans sulphate: 53,61% (dry basis); Chondroitin sulfate determined by HPLC: 37,32% (dry basis) Keywords: Chondroitin sulfate, Glycosaminoglycan, Alcalase, chicken keel cartilage, hydrosis * * 117 (2017) 086-092 2.3.2 nh h at tính c a enzyme Alcalase 2.4L o o Carcharhinus 2.3.3 ng GAGs sulfate Sorrah Dasyatis Kuhlii 2.1 V t li u o Bacillus Licheniformis 2.3.4 Phân tích th ng kê s li u 2.2 B trí thí nghi m 3.1 Thành ph o pro 2.3 2.3.1 tích nh thành ph n c a s n c gà n c a s n c gà 117 (2017) 086-092 3.3 GAGs ng c a pH n ng GAGs GAGs 3.2 ng c a t l s c n ng GAGs GAGs GAGs GAGs GAG GAGs 117 (2017) 086-092 3.4 ng c a nhi n ng thu GAGs 0 GAGs 0 3.7 T ut th y phân s n c gà n trình GAGs 0 3.5 ng c a ng th t n pro pro GAGs k pro 0 pro 3.6 ng c a th i gian n ng thu GAGs GAGs GAGs 12 2 1 13 2 2 1 14 2 3 2 3 23 2 4 24 4 34 3 2 3 2 117 (2017) 086-092 2 1 2 3 Source pro Sum of Mean Degree of Squares Squares Freedom (DF) (SS) (MS) F value P value 0.000 Regression 14 145.087 10.3634 22.274 Residual 16 7.4442 0.465262 Lack of Fit 10 6.45082 0.645082 3.8963 Pure Error 0.99337 0.165562 Total Corrected 30 152.531 0.055 Có Khơng 5.08437 3.8 K t qu ki m ch ng giá tr d HLGAGs (%) t mơ hình v i giá tr th c nghi m 4 pro GAGs 117 (2017) 086-092 pro 117 (2017) 086-092 [5] D Uebelhart et al (2004) Intermittent treatment of knee osteoarthritis with oral chondroitin sulfate: a one-year, randomized, double-blind, multicenter study versus placebo Osteoarthritis Cartilage, vol 12, 269-276 [6] M.T Osterman et al (2007) Current and future antiTNF therapy for inflammatory bowel disease Curr Treat Options Gastroenterol, vol 10, 195-207 [7] J.W Cho et al (2007) Curcumin attenuates the expression of IL-6, and TNFcyclin E in TNF- -treated HaCaT cells; NFMAPKs as potential upstream targets International journal of molecular medicine, vol 19, 469-474 pro [8] C Jomphe et al (2007) Chondroitin sulfate inhibits the nuclear translocation of nuclear factor-kappaB in interleukin-1beta stimulated chondrocytes Basic Clin Pharmacol Toxicol, vol 102, 59-65 [9] kuhlii) cá nhám ( , vol 16, 26-30 [10] W.R Farndale, D.J Buttle, A.J Barrett (1986) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue Biochimca Biophysica Acta 883, 173 177 [11] S.C Shin et al (2006) Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage AsianAustralasian Journal of Animal Sciences, vol 19, 601-604 Tài li u tham kh o [1] Johanne Martel-Pelletier, Aina Farran, Eulàlia Montell, Josep Vergés and Jean-Pierre Pelletier (2015) Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate Molecules 20, 4277-4289 [12] C.Y Cheng et al, "Optimization of microwaveassisted extraction of chondroitin sulfate from tilapia byproduct by response surface methodology," Consumer Electronics, Communications and Networks (CECNet), 2011 International Conference on, pp 1462-1465, 2011 [2] J.C.F Kwok et al (2012) Chondroitin sulfate: A key molecule in the brain matrix The International Journal of Biochemistry & Cell Biology, vol 44, 582586 [13] C Kun-Nan and C Ming-Ju, "Statistical Optimization: Response Surface Methodology Optimization in Food Engineering," in Optimization in Food Engineering, Ferruh Erdogdu, Ed New York, USA: Taylor & Francis Group, 2008, ch 6, pp 115141 [3] Tadahisa Mikami et al (2013) Biosynthesis and function of chondroitin sulfate Biochimica et Biophysica Acta, vol 1830, 4719 4733 [14] AOAC, Official Methods of Analysis: The Association of Offiial Analytical Chemists Washington DC, USA, 1999 [4] D Uebelhart et al (1998) Effects of oral chondroitin sulfate on the progression of knee osteoarthritis: a pilot study Osteoarthritis and cartilage, 39-466 ... (2017) 086-092 2.3.2 nh h at tính c a enzyme Alcalase 2.4L o o Carcharhinus 2.3.3 ng GAGs sulfate Sorrah Dasyatis Kuhlii 2.1 V t li u o Bacillus Licheniformis 2.3.4 Phân tích th ng kê s li u 2.2 B... Effects of Different Formulations of Chondroitin Sulfate Molecules 20, 4277-4289 [12] C.Y Cheng et al, "Optimization of microwaveassisted extraction of chondroitin sulfate from tilapia byproduct by... c a nhi n ng thu GAGs 0 GAGs 0 3.7 T ut th y phân s n c gà n trình GAGs 0 3.5 ng c a ng th t n pro pro GAGs k pro 0 pro 3.6 ng c a th i gian n ng thu GAGs GAGs GAGs 12 2 1 13 2 2 1 14 2 3 2 3