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Cụng ty TNHH Bayer Vit Nam CNG HềA X HI CH NGHA VIT NAM c lp T Hnh phỳc BO CO TểM TT NH GI RI RO CA THC VT BIN I GEN I VI SC KHE CA CON NGI V VT NUễI BễNG CHNG CHU THUC TR C GLYPHOSATE GHB614 CễNG TY TNHH BAYER VIT NAM THNG 4, 2015 Ton b d liu h s ng ký ny c bo v bn quyn v khụng c s dng, chộp hay a cho bờn th ba nu khụng cú c s ng ý ca cỏc tỏc gi Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN I THễNG TIN CHUNG T chc cỏ nhõn ng ký: - Cụng ty TNHH Bayer Vit Nam Ngi i din ca t chc: ễng David John Champion Tng Giỏm c u mi liờn lc ca t chc: TS ng Ngc Chi Giỏm c ng ký ht ging a ch: Lụ 118/4 Khu cụng nghip Amata, Thnh ph Biờn Hũa, Tnh ng Nai in thoi: (+84-8) 3845 0828 Fax: (+84-8) 3997 9206 Email: chi.dang@bayer.com Tờn thc vt bin i gen - Tờn thụng thng: Bụng Tờn khoa hc : Gossypium hirsutum Tờn thng mi: GlyTol Tờn s kin chuyn gen: GHB614 Tớnh trng liờn quan n gen c chuyn: Chng chu thuc tr c glyphosate Mó nhn din nht (nu cú): BCS-GHỉỉ2-5 Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN II THễNG TIN V CY CH NHN GEN Tờn cõy ch nhn gen: (a) Tờn khoa hc: Gossypium hirsutum (b) Tờn thụng thng: Cõy bụng (c) V trớ phõn loi: Tc: Gossypieae H: Malvaceae Chi: Gossypium Loi: hirsutum Thụng tin liờn quan n sinh vt nhn Lch s canh tỏc v phỏt trin Bụng, tờn khoa hc l Gossypium hirsutum, c trng nhiu thiờn niờn k nhiu ni trờn th gii Khong 90% lng bụng th gii l G hirsutum Bụng ch yu c s dng rng rói nh si ca nú X si c to trờn v ht vũng quanh thnh si chc mnh Ch cú M v mt vi nc khỏc phỏt trin cỏc sn phm thng mi t ht bụng Ht bụng thụ cú th c s dng lm thc n cho i gia sỳc dng cỏc khụ ht bụng, v ht hoc ht cú th c ch bin thu du, mt thnh phn chớnh c s dng lm thc phm Linters (x ngn) cũn li trờn v sau loi b si di cú th s dng cho c mc ớch thc phm/thc n chn nuụi hoc cỏc mc ớch khỏc Bụng thuc vo chi Gossypium, nm h Malvaceae hoc Mallow Cỏc thnh viờn khỏc ca h ny bao gm mp tõy, thc qu, hoa hng sharon, hay thm l cỏc cõy trng nh c tr li l nhng cõy xp vo nhúm c cú anh hng xu ti bụng Ch cú chi Gossypium v mt vi chng phõn lp khỏc thuc cỏc chi khỏc, c xỏc nh c tớnh thụng qua si (lụng), ht lụng (seed hair) hay cu trỳc to lụng, phỏt trin ngoi biu bỡ ca v ht Cú 50 loi phõn b chi Gossypium, nhng ch s chỳng c s dng sn xut x vi thng mi (Fryxell, 1992) Nhúm Gossypieae cú c im c trng: s to thnh phụi (phc hn so vi cỏc nhúm khỏc h Malvaceae) v s cú mt ca cỏc mch h khỏc bit nhiu phn ca cõy nhng c bit lỏ mm Cỏc mch h c bit l cỏc tuyn cha gossypol v khỏc bit v c im sinh thỏi v thnh phn hoỏ hc õy l nhng c im c trng cho nhúm ny (Fryxell, 1979) Bụng cú ngun gc t cõy g lõu niờn, sau ú c thun hoỏ v chuyn thnh cõy trng thu hoch hng nm v thng khụng mang cỏc c im ca cõy thuc nhúm c di Bụng c trng t ht Nu vng mt cỏc cụn trựng h tr th phn, bụng cú th t th phn, nhng cỏc tỏc nhõn th phn cú mt, vic th phn chộo s din ỏng k (Oosterhuis v Jernstedt, 1999) Vic trng bụng b hn ch v mt a lý hn bt k cõy trng no khỏc Chõu u Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt bi nú ch cú th phỏt trin c cỏc vựng cú di 180 ngy lnh giỏ nm (180 frost-free days per year) Bụng cn lng nc ma trung bỡnh ớt nht l 50 cm/nm, nhng ma ln v thi tit cú m ln giai on cui quỏ trỡnh phỏt trin d lm gim nng sut cng nh tng nguy c tn cụng ca sõu bnh.Ti thi im thu hoch, thi tit cn khụ rỏo, ma s lm bin mu si v nh hng ti cht lng x vi Bụng c trng v thu hoch M mt cỏch c gii hoỏ Cỏc k thut trng, bao gm ti tiờu v luõn canh cõy trng, thuc tr c c s dng kim soỏt c di Vic kim soỏt c di cú nh hng ln ti nng sut trng bụng, ú hu ht cỏc vựng trng bụng M u s dng thuc tr c Ngi trng bụng cú th s dng thuc tr c vi ph tỏc dng rng ti nhiu loi c di, nhng khụng nh hng ti cõy bụng, cú tớnh kinh t v khụng nh hng ti mụi trng Nhiu loi thuc tr c cú ph tỏc dng rng, nhng cú th tiờu dit bụng c vi lng khuyn cỏo cho vic kim soỏt c Cỏc c im tớnh trng cung cp thụng tin liờn quan n tớnh bn ca cỏc ging mi c phõn phi thng mi húa Vic chn lc cỏc ging mi ban u da vo cỏc d liu ca cõy trng b m Cỏc nh chn to ging ỏnh giỏ cỏc ging bụng mi chn to qua nhiu ch tiờu ti cỏc thi k sinh trng khỏc ton b quỏ trỡnh phỏt trin ging giai on sm ca quỏ trỡnh sinh trng, cỏc nh to ging ỏnh giỏ s cõy tn ti v khe ca cõy ging Khi cõy bc vo thi k chớn, kh nng khỏng sõu v bnh c tip tc ỏnh giỏ Ht bụng thu hoch c phõn tớch cỏc ch tiờu nh nng sut, si bụng di v khe Trong mt s trng hp, cõy trng c ci to nõng cao mt s thnh phn c bit, v cỏc nh chn to ging cõy trng thng chỳ ý phõn tớch cỏc thnh phn ú Thụng tin v s an ton ca cõy nhn gen Bụng khụng c coi l cõy trng gõy hi, gõy d ng v gõy bnh cho ngi, nhiờn nú to cỏc cht khỏng dinh dng nh gossypol v axit bộo cyclopropenoid (CPFA) Quỏ trỡnh ch bin ht bụng cú th bao gm cỏc cụng on: tỏch si, tỏch v, nghin, ộp tm, chit, ty mu, ty mựi thu du bộo thc phm Sn phm ph ca quy trỡnh ch bin cú th s dng lm thc phm cho ngi nh x ngn, hay thc n chn nuụi nh v hay khụ du bụng (Freyssinet, 2007; OECD,2009) Du ht bụng c sn xut u tiờn Hoa K Du thụ cú cha khong 2% cỏc vt liu nonglyceride nhng c loi b gn nh hon ton quỏ trỡnh ch bin Trong cỏc vt liu ny cú phytoalexin, axit bộo cyclopropenoid (CPFA), phospholipid, cỏc sterol, resins, carbohydrate v cỏc sc t liờn quan Thnh phn ỏng chỳ ý nht ca terpenoid phytoalexin l gossypol Quỏ trỡnh ch bin du loi tr hu ht thnh phn gossypol, quỏ trỡnh kh mựi s loi b hu ht cỏc CPFA Khụ du ht bụng, v, x ngn bụng l cỏc sn phm ph ca ngnh cụng nghip du ht bụng Trong nhng sn phm ny, khụ du ht bụng l nhiu nht v c sn xut bng cụng ngh ộp du v s dng húa cht tỏch chit cú th s dng lm thng phm vi cỏc Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt sn phm cú hm lng gossypol thp, gii hn ca thnh phn gossypol l di 0,04% hay (400 ppm) X ngn bụng hu ht ch cha cellulose tinh cht X ngn bụng cú cht lng cao nht c tinh sch cỏc x lý húa hc tiờu húa, ty ra, git v lm khụ V cú hm lng ln cỏc cht x khụng tiờu húa c Gossypol Bụng cú cha mt lng tng i cỏc cht terpenoid phytoalexin Cỏc Phytoalexin l cỏc cht khỏng sinh, bụng thỡ cỏc cht ny c tớch tr cỏc tuyn sc t Cỏc cht ny úng vai trũ quan trng vic chng chu vi mt s loi bnh cú th gõy hi trờn cõy bụng Cht terpenoid phytoalexin thụng dng cõy bụng gm cú gossypol, hemigossypol, desoxyhemigossypol, 2,7-dihydroxy cadalene, hemigossypolone v heliocides H1 v H2 Gossypol l cht ỏng chỳ ý nht cỏc cht terpenoid phytoalexin v c phõn lp u tiờn t cỏc tuyn sc t ca ht bụng Cht ny nhỡn chung l c tc cho cỏc loi khụng phi ng vt nhai li v mang chc nng bt dc c Gossypol cú th thy dng t hoc kt hp Nghiờn cu cho rng khong 18mg gossypol t (tng ng vi 0,1% gossypol t do) l hm lng cao nht m bũ sa cú th c cho n hng ngy Cỏc nh khoa hc cng ó tỡm rng gossypol l cht cú th chng virus v chng li ung th Cỏc axit bộo Cyclopropenoid Bụng cha mt s axit bộo cyclopropenoid (CPFA) cú du Cỏc loi cú th o t c gm cú axit malvalic, sterculic v dihydrosterculic Cỏc CPFA nõng im hũa tan (melting point) ca cỏc cht bộo ng vt c cho n bng ht bụng v khụ du ht bụng C ch ca cỏc hot ng ny xut hin c ch ca vic lm khụng no cỏc axit bộo no bóo hũa Trong g, lũng trng mt mu v gim kh nng n l hai nh hng bt li, v ú, cỏc ngnh cụng nghip rt hn ch vic s dng khụ du ht bụng ch dinh dng cho gia cm Cỏc CPFA cng cú s liờn kt vi cỏc t l cao ca ung th gan cỏ hi cho n ht bụng, mc dự ó bit rng aflatoxi, mt c t mycotoxin b nhim ht bụng, cng gõy ung th i vi cỏ hi cu vng (rainbow trout) Cỏc thnh phn khỏc Lỏ cõy bụng mang mt s cht flavonoid, cỏc tannin v anthocyanin Mt s lỏ bụng c thu hoch cựng vi qu bụng v b loi b quỏ trỡnh ta ht (ginning) Trong mt s iu kin ngoi l, nh iu kin hn, cỏc phn loi b sau ta ht hay gc cõy bụng c s dng lm thc n chn nuụi cho i gia sỳc Tuy nhiờn, vỡ hn ch vic s dng, flavonoid, tannin v anthocyanin khụng c cho l cỏc cht chng dinh dng chớnh hay cỏc cht c t nhiờn (OECD, 2009) Lch s s dng cõy ch lm thc phm, thc n chn nuụi Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt Bụng, Gossypium spp c trng thu si t hng ngn nm Vic trng v sn xut vi si c phỏt trin c lp gia bỏn cu Nam v Bc Mt nhng ghi chộp lõu i nht v cụng nghip dt may thỡ cỏch õy 5000 nm cụng nghip vi si t bụng ó cú thung lng sụng Indus, thuc Pakistan Kho c Peru v Mexico ó phỏt hin nhiu mu vi bụng cú ti 4000 -7000 nm tui Vi bụng cng c tỡm thy nhiu nn minh Ai Cp c hay cỏc du tớch ca ngi da Indian khu vc Tõy Nam nc M t hng trm nm trc cụng nguyờn Ht bụng, sn phm nụng nghip thụ trc õy phn ln c loi b, c chuyn thnh thc phm cho ngi, ng vt, phõn bún cho thc vt, si cho sn xut tm lút ni tht, cellulose cho nhiu ngnh cụng nghip khỏc t thuc n ti bo mch chip mỏy vi tớnh Ngoi bụng l thc phm ca t nhiờn v cõy trng to si Mc dự si bụng l sn phm cú giỏ tr nht lnh vc bụng vi nhng cng phi chỳ ý rng cõy trng ny cng l ngun cung cp du thc vt quan trng iu ny cú ngha, bụng cng l cõy trng s dng cho mc ớch thc phm Bụng c sn xut chớnh Trung Quc, M, n , Pakistan v Uzbekistan, vi tng sn lng chim gn 75% sn lng ton th gii Ch cú qu nang ca bụng l c dựng thu si hoc lm thc phm/thc n chn nuụi Phn cũn li ca cõy c s dng lm phõn bún ti rung trng sau quỏ trỡnh phõn hu (OECD, 2009) Ht bụng l ngun cung cp du bộo v protein rt tt bi hm lng cao cỏc thnh phn ny ht cng nh cht lng axit bộo v axit amin ca nú Bn sn phm chớnh t quỏ trỡnh ch bin ht bụng l du bộo, khụ du, v v x ngn bụng T l cỏc sn phm cụng nghip ch bin ht bụng M vũng nm gm cú: 16,14% du, 25,15% v ; 46,0% khụ du bụng; 8,51% x ngn v 4,21% cht thi (Jones v King, 1993) Ngy bụng ti cỏc nc cụng nghip phỏt trin c thu hỏi mt cỏch c gii hoỏ Sau tỏch bụng, nang qu bụng thng c ộp c hc, chuyn vo dõy chuyn ch bin c gi l quy trỡnh ta bụng Trong quỏ trỡnh ta nang qu bụng, si cho cụng nghip dt may c tỏch riờng t ht Ht ó c tỏch bụng s c gi l ht x (fuzzy seed) (hay cú th gi l ht tỏch si di), s c s dng ch bin hoc dựng trc tip lm thc n gia sỳc Du bụng l ngun du thc vt quan trng, õy l mt cỏc loi du bộo cha nhiu axit cha bóo ho nht, cựng vi du ci, ngụ, bụng , hoa rum , hng dng, du bụng cng cú cht lng cao bi hm lng vitamin E v bn ca nú c s dng lm du rỏn X x ngn ht bụng (cottonseed linter fiber), bao gm trờn 99% l cht x cng c dựng lm thc phm sau c x lý hoỏ hc phõn gii, lm trng, v sy khụ Sn phm ny dng bt trng, khụng mựi, mu v bn hoỏ hc vy khụng phn ng vi cỏc thnh phn khỏc gõy bin mựi, mu cho thc phm Do vy nú cú th c dựng nhiu thc phm nh bỏnh, dressing, n nhanh hoc tht ch bin Khụ du ht bụng Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt Phng phỏp ch bin du bộo nh hng ti tớnh hu dng ca khụ du bụng (cottonseed meal) So vi cỏc phng phỏp ch bin khỏc, vic ộp vớt ti gim hm lng gossypol t do, gim Nit ho tan nhng li cú c hm lng du ln v tiờu tn nng lng S dng cho nuụi g, hm lng du cao cú th nh hng ti s mt mu ca trng quỏ trỡnh bo qun (do s cú mt ca axit bộo cyclopropenoid); i vi gia sỳc, hm lng du cao to nhiu nng lng cho vt nuụi Phng phỏp chit bng dung mụi trc tip s to khụ du bụng cú hm lng gossypol t v nit ho tan cao Nu da trờn quan im v cht lng protein thỡ khụ du bụng sn xut theo phng phỏp ny rt tt cho ng vt cú d dy n (ng vt nhai li) Phng phỏp ch bin s dng dung mụi tỏch chit cú ộp s b to sn phm khụ du bụng cú c im trung gian gia phng phỏp tỏch du l ộp v s dng húa cht Nú cú hm lng gossyol t thp, ho tan nito cao hn mc trung bỡnh, hm lng cht bộo thp Loi khụ du bụng ny c s dng cho g trng (Ensminger et al., 1994) Ht bụng c ch bin thnh cỏc khụ du bụng cú cha hm lung protein khỏc 36%; 41%; 44% v 48-50% Khụ du bụng cú cha 36% protein thu c t phng phỏp ộp bng trc vớt, ú phng phỏp chit dung mụi trc tip hoc chit dung mụi trờn ht bụng ó c ộp s b to khụ du bụng cú hm lng protein cao hn 44% (Ensminger v cng s, 1994) Ngoi mc ớch s dng cung cp protein cho cỏc sn phm thc phm/thc n chn nuụi, khụ du bụng cũn l ngun cung cp protein k thut v nú c s dng lm cht kt dớnh Protein ny c ộp ựn to vt liu si s dng cho cụng nghip dt may Cỏc sn phm protein t ht bụng Trc õy, sn phm protein t ht bụng c s dng ch yu cho mc ớch phõn bún hoc lm thc n chn nuụi T u nhng nm 60, ngi ta bt u mi phỏt trin cỏc sn phm protein t ht bụng lm thc phm cho ngi Sn phm u tiờn c thng mi hoỏ l bt ht bụng s dng b sung vo bỏnh mỡ hay mụi trng nuụi cy vi sinh vt (trong cỏc nghiờn cu) Nam M, thc phm n dm cha hm lng protein cao cú tờn l Incaparinha c sn xut s dng cho tr thiu dinh dng õy l hn hp gia bt ngụ v ht bụng vi t l 60:40 (Bressani, 1997) X ngn ht bụng (Cottonseed linters) Ba loi x ngn bụng chớnh c thu hi quỏ trỡnh tỏch si: cụng on ct th nht: 0,15-0,38 kg / 10 kg ht; cụng on ct th 2, 0,90-1,05 kg / 10 kg ht; v thu t cụng on nghin, 0,35-1,00 kg / 10 kg ht Sn phm t cụng on ct th nht c s dng to cỏc sn phm s dng cho phu thut, giy, bao gúi; sn phm t cụng on ct th s dng sn xut si tỏi sinh, phim nh, thuc n, cht do, giy, v sn phm thu t cụng on nghin s dng cụng nghip to cellulose hoỏ hc v cỏc sn phm nm lút (Cherry v Leffler, 1984) Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt X ngn bụng - Linter, cha 90-95% cellulose, khụng cha lignin, cú hm lng sỏp thp, pectin, axit hu c, cỏc cht tro vụ c (Jones v King, 1993) Sau quỏ trỡnh ch bin pH kim, v nhit cao, x ngn t ht bụng cú th c s dng nh l mt sn phm cú hm lng cht x cao Cht x ny dựng cho thc phm cú cha trờn 99% hm lng s tng (Freyssinet, 2007) V ht V l phn bờn ngoi ht cú tỏc dng bo v ht, phn ny phi c tỏch l phn bờn ht cho quỏ trỡnh tỏch chit du S cú mt v ht khụ ht lm gim hiu sut thu hi du cú th hp ph mt lng du t Khong 1% ca v ht tớnh theo lng l du v 3% l protein V cú cha hm lng cht x cao, c bit l cellulose v lignin (NCPA, 2000; Jones v King, 1993) V ht bụng c s dng lm thc n thụ cho chn nuụi, hay cú th dựng lm nhiờn liu, vt liu cỏch nhit, lm m t, s dng du khoan, b sung vo cht cha phenol tng bn, cung cp cellulose cho cỏc loi x tỏi sinh v cng l ngun to xylose v furfural (Cherry v Leffler, 1984) Lecithin Photpho lipit Trong cỏc loi du t ht núi chung, tr bụng thỡ ht bụng cú hm lng photpho lipit l cao nht Phõn on cha lng photpho lipit thu t quỏ trỡnh tinh ch du bộo c x lý loi cỏc lipit trung tớnh v cỏc protein tp, sau ú c phõn on tip thnh photpho lipit v glycolipit Do s cú mt ca gossypol liờn kt vi photpho lipit quỏ trỡnh tỏch du, photpho lipit t ht bụng ch c s dng cho cỏc mc ớch cụng nghip Photpho lipit bụng cú nhiu u im hn so vi photpho lipit t cỏc loi ht khỏc bi hu ht cỏc axit bộo cú mt u ch cú di liờn kt ụi vy chỳng ớt b oxy hoỏ v b ụi hn (Cherry v Leffler, 1984) T ging bụng khụng to gossypol (glandless cotton), cú th cú c sn phm photpho lipit t ht bụng dựng cho thc phm (Cherry v Leffler, 1984) Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN III THễNG TIN LIấN QUAN N SINH VT CHO GEN Tờn ca sinh vt cho gen Tờn khoa hc: Zea mays Tờn thụng thng: Ngụ, bp V trớ phõn loi: Tc: Maydeae H: Poaceae Chi: Zea Loi: mays Loi ph: mays Thụng tin liờn quan n sinh vt cho gen S kin chuyn gen bụng GHB614 mang mt tớnh trng n l chng chu thuc tr c gen 5-enol-pyruvylshikimate-3-phosphate synthase b bin i, 2mepsps, cú ngun gc t ngụ c chốn vo h gen cõy bụng Ngụ cú mt lch s s dng an ton cho vic trng trt cng nh s dng lm thc phm v thc n chn nuụi Ngụ l loi cõy ng cc ng th ba trờn th gii sau lỳa m v go Ngụ c trng thng mi rng rói ti trờn 25 quc gia Ngụ cụng nghip c trng t khong 8000 nm trc ti Mexico v Trung M v khong 500 nm Chõu u T nhng nm 1995 1997, 66% ngụ sn xut trờn th gii c dựng lm thc n chn nuụi v 17% cho ngi tiờu th cỏc nc ang phỏt trin, 30% ngụ c sn xut lm thc phm v khong 57% lm thc n chn nuụi, ú Chõu u, Bc M v cỏc quc gia thu nhp u ngi cao, 4% ngụ c s dng cho ngi tiờu th v 76% l cho thc n chn nuụi Ngụ cú lch s s dng an ton lm thc phm v thc n chn nuụi Mt lng khụng ỏng k ca cỏc cht chng dinh dng v gõy d ng cú mt ht ngụ c loi b qua quy trỡnh ch bin thụng thng cụng nghip Ngụ t nhiờn ó mang cỏc chng dinh dng v cht gõy d ng Axit phytic cú mt ngụ tn ti dng liờn kt vi phụtpho vo khong 60-70% (phytate) Do tn ti ch yu dng phytate, nờn lng cung cp phtpho sn cú t nhiờn ngụ ch cú di 15% cho ng vt khụng nhai li Thụng thng ngi ta thng a thờm phytase vo khu phn dinh dng cho ln v gia cm nõng cao vic s dng phtpho Thnh phn axit phytic ht ngụ thay i t 0,45 1% trng lng khụ Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt Raffinose l mt oligosaccharide khụng tiờu hoỏ c Raffinose c xp vo mt cht khỏng dinh dng vic to khớ v lm cho y hi s dng thnh phn ny Raffinose khụng phi l cht gõy c nhng cú th gõy s khú chu Cú th loi tr thnh phn ny thc phm v thc n chn nuụi bng cỏch ngõm, nu, x lý vi enzyme, dung mụi hay tia hng ngoi T l ca raffinose ngụ l 0,21 0,31%; hm lng tiờu th mi ngy 15g c cho l an ton Ngụ mang hm lng thp cỏc cht c ch trypsin v chymotrypsin, khụng cht no cú ý ngha v mt dinh dng Ngụ thụng thng khụng phi l mt loi thc phm gõy d ng, dự vi trng hp, mt s phn ng d ng ó c ghi nhn Cỏc bỏo cỏo v nh hng d ng ca ngụ gm cú d ng trờn da, h tiờu hoỏ, v cỏc v h hụ hp (OECD, 2002) Ngụ cú mt lch s s dng an ton cho vic trng trt cng nh s dng lm thc phm v thc n chn nuụi Ngụ l loi cõy ng cc ng th ba trờn th gii sau lỳa m v go Ngụ c trng thng mi rng rói ti trờn 25 quc gia Ngụ cụng nghip c trng t khong 8000 nm trc ti Mexico v Trung M v khong 500 nm Chõu u T nhng nm 1995 1997, 66% ngụ sn xut trờn th gii c dựng lm thc n chn nuụi v 17% cho ngi tiờu th cỏc nc ang phỏt trin, 30% ngụ c sn xut lm thc phm v khong 57% lm thc n chn nuụi, ú Chõu u, Bc M v cỏc quc gia thu nhp u ngi cao, 4% ngụ c s dng cho ngi tiờu th v 76% l cho thc n chn nuụi Ngụ mt kh nng tn ti ngoi t nhiờn quỏ trỡnh thun hoỏ lõu di v cn cú s can thip ca ngi phỏt tỏn ht cho nú Mc dự ngụ t cỏc v trc cú th chng chu qua ụng v ny mm vo nm tip theo, nhng nú khụng th sng c nh c di Ngụ khụng cú kh nng tỏi sinh ngoi iu kin trng trt ó c thun hoỏ Cõy ngụ khụng phi l loi xõm ln t nhiờn Trỏi ngc vi c di, ngụ cú nhy hoa cỏi (bp) vi bp c bao bc bi cỏc lỏ bi Chớnh vỡ vy m vic phỏt tỏn ht n l khụng xy mt cỏch t nhiờn (OECD, 2003) Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN IV THễNG TIN V THC VT BIN I GEN Mụ ta qua trinh tao thc vt biờn ụi gen Quỏ trỡnh chuyn gen on ADN t plasmid pTEM2 c chuyn vo h gen ca bụng GHB614 bng vi khun Agrobacterium Vi phng phỏp chuyn gen ny, on ADN nm gia b phi v b trỏi ca T-DNA t plasmid pTEM2 c chuyn lờn h gen ca cõy trng Cỏc vựng b phi v trỏi ca A tumefaciens, nh mụ t bng cng c chốn vo h gen bụng s kin Glytol GHB614 Mc dự mt s gen s dng quỏ trỡnh chuyn gen cú xut x t A tumefaciens, c bit l vi sinh vt gõy bnh cho cõy, to u cho cõy c loi b v khụng hp nht vo h gen cõy nhn (Deblaere v cng s, 1985) Gen 2mepsps c s dng mt s cõy trng nụng nghip to kh nng khỏng thuc tr c glyphosate cho cõy trng Cỏc thụng tin chi tit v quỏ trỡnh chuyn gen, s nhn din cỏc vt liu di truyn a vo cõy ch, phõn tớch cỏc d liu xỏc inh c tớnh ca on chốn c mụ t cỏc phn IV.A.2a, IV.A.2.b, v IV.A.2.d, di õy Phng phỏp chuyn gen Mu mụ Gossypium hirsutum t bụng Coker312 c chuyn gen vi vector pTEM2 (Habex, 2011) thụng qua phng phỏp chuyn gen s dng Agrobacterium tumefaciens T bo mụ bụng c tip xỳc vi canh trng chng A tumefaciens C58C1Rif ó c lm mt c tớnh (Van Larebeke v cng s, 1974) cú cha vector pTEM2 Sau quỏ trỡnh cựng nuụi cy, cỏc t bo mụ bụng c tỏi sinh thnh cõy trờn mụi trng cú s dng 500 mg/l claforan tiờu dit Agrobacterium cũn li, sau ú tip tc c chn lc vi glyphosate Cỏc chi cõy to c chuyn vo nh kớnh, sau ú c ỏnh giỏ kh nng khỏng thuc tr c glyphosate, c tip tc trng ti hoa v to ht Nờu nhng tinh trng v c iờm mi ca thc vt bin ụi gen so vi loi thc vt thụng thng tng ng Tớnh trng c a vo cõy bụng l tớnh chng chu vi thuc tr c glyphosate Bụng GHB614 cha gen ci bin 2mepsps c hp nht n nh vo h gen cõy trng, gen ny mó hoỏ cho enzyme ci bin 5-enolpyruvylshikimate 3-phosphate synthase (2mEPSPS) Glyphosate l loi thuc tr c rng rói cú tỏc ng c ch cỏc enzyme t nhiờn 5enolpyruvylshikimate 3-phosphate synthase (EPSPS), tham gia vo quỏ trỡnh chuyn hoỏ axit shikimic sinh tng hp axit amin vũng thm cõy trng v vi sinh vt (OECD, 1999) Tuy nhiờn, enzyme 2mEPSPS nh s bin i cú ch ớch trỡnh t, nú khụng b c ch bi glyphosate v s biu hin ca nú to hot tớnh m bo s khỏng li thuc tr c glyphosate cho bụng GHB614 Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt S thay i kiu hỡnh tng ng vi s chuyn gen ú l bụng GHB614 phỏt trin tt s dng thuc tr c glyphosate Tớnh trng ny khụng nh hng ti mụi trng vụ sinh cng nh hu sinh xung quanh Thụng qua cỏc nghiờn cu kho nghim trờn rung ca bụng GHB614, vic chuyn vo on chốn cha gen 2mepsps khụng cú cỏc nh hng a kiu hỡnh gõy s khỏc bit no c quan sỏt c Bụng mang s kin GHB614 khụng khỏc bit so vi cỏc dũng b m hay cỏc bụng thụng thng khỏc v cỏc c im dinh dng, nụng hc v kh nng sinh sn Lch s cp phộp lm thc phm, thc n chn nuụi Bụng GHB614 ó c cp chng nhn an ton cho vic s dng lm thc phm v thc n chn nuụi cng nh an ton cho mụi trng 10 quc gia Cỏc quc gia ó cp chng nhn an ton cho s kin GHB614 Thc phm Quc gia Australia New Zealand Brazil Canada Trung Quc Colombia Liờn minh Chõu u Nht Bn Mexico Hn Quc Hoa K x x x x x x x x Thc n chn nuụi x x x x x x x Nm cp 2009 2010 2008 2010 2012 2011 2010 2008 2010 2009 n v cp chng nhn FSANZ CTNBio, CNBS CFIA, CaH MOA INVIMA, ICA EFSA MHLW, MAFF SdS MFDS (KFDA); RDA FDA, USDA Vit tt: FDA: B lng thc v thuc Hoa K USDA: B Nụng nghip Hoa K MFDS: B An ton thc phm v thuc, Hn Quc (Tờn gi trc õy l KFDA: Korea Food and Drug Administration) RDA: B Nụng nghip nụng thụn Hn Quc MHLW: B Y t, lao ng v phỳc li Nht Bn MAFF: B nụng nghip, lõm nghip v ngh cỏ INVIMA: Institute Nacional de Vigilancia de Medicamentos y Alimentos FSANZ: C quan tiờu chun thc phm MOA: B nụng nghip CFIA: C quan kim tra thc phm Canada CaH: B Y t Canada EFSA: C quan an ton thc phm liờn minh chõu u SdS: Secretaria de Salud CTNBio: Hi ng cụng ngh quc gia v an ton sinh hc (Comissóo Tộcnica Nacional de Biosseguranỗa) CNBS Hi ng b trng ICA: Instituto Colombiano Agropecuario Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN V NH GI NGUY C NH HNG CA THC VT BIN I GEN I VI SC KHE CON NGI V VT NUễI Ngoi tớnh trng khỏng glyphosate c a vo cú ch ớch, cú th thy khụng cú bt k thay i no khỏc hoc cú thay i xut hin mt cỏch khụng ch ớch v thnh phn ca bụng GHB614 so vi i chng khụng chuyn gen Coker 312 Cỏc d liu v axit bộo, proximate, axit amin, vitamin E, cht chng dinh dng u tng ng gia bụng chuyn gen v i chng khụng chuyn gen Coker 312 So sỏnh v mt dinh dng gia bụng chuyn gen GHB614 v i chng khụng chuyn gen Coker 312 Mc ớch s dng c bn ca bụng l cho dt may Tuy nhiờn cỏc sn phm ph thu ca bụng t cụng nghip s si cú th c s dng lm thc phm hoc thc n chn nuụi Thnh phn dinh dng ca ht, x ngn v cỏc sn phm ch bin khỏc c phõn tớch v cho thy s tng ng v thnh phn vi cỏc sn phm tng ng thu t bụng i chng khụng chuyn gen Coker312 (Currier, 2007) S tng ng v mt dinh dng cho thc phm v thc n chn nuụi ca bụng GHB614 c ỏnh giỏ v c kt lun l bụng GHB614 khụng cú cỏc tỏc ng bt li ti ngi v vt nuụi c s dng ỳng mc ớch bi nhiu c quan chc nng mt s quc gia trờn th gii nh Chõu u, Hoa K, c, New-Zealand, Nht Bn Cỏc sn phm ph ca quỏ trỡnh ch bin ht bụng (v v khụ du bụng) cú th c s dng lm thc n chn nuụi Bụng cha mt s thnh phn chng dinh dng, hu ht l trung khụ du bụng Cỏc thnh phn ny gm gossypol v axit bộo cyclopropenoid, u d b bin tớnh bi nhit Khụ du bụng thng tri quỏ quỏ trỡnh x lý nhit loi nc v d cho vic tỏch du Vic x lý ny s vụ hot protein v loi b nh hng ca gossypol Cỏc thnh phn khỏng dinh dng thụng thng ca bụng c xỏc nh nhiu nht sn phm khụ du bụng cú sy v hm lng cỏc thnh phn ny u thp hn ngng cho phộp T l úng gúp ca bụng chuyn gen GHB614 vo thc n chn nuụi cng c ỏnh giỏ Hm lng cao nht ca protein 2mEPSPS l 9,6 g / g thc n c xỏc nh cho bũ v ln Tuy nhiờn, tớnh toỏn ny da trờn trng hp 100% thc n chn nuụi c ch bin t bụng GHB614 (Oberdửrfer, 2010c) Phõn tớch thnh phn nguyờn liu thụ ó c tin hnh (Oberdửrfer, 2010a ) theo hng dn ca OECD i vi cỏc thnh phn dinh dng chớnh bụng (OECD, 2009) Kt lun v s tng ng v thnh phn v dinh dng gia bụng chuyn gen v i chng khụng chuyn gen cng nh vi bụng thng mi ó c a Khụng cú nh hng no ca gen c chốn vo ti giỏ trnh dinh dng ca ht bụng, du, khụ v v ht bụng Vic thờm gen mi vo h gen ca bụng khụng lm thay i thnh phn v cht lng dinh dng Trờn phng din dinh dng, thc phm v thc n chn nuụi t bụng GHB614 ging vi thc phm v thc n chn nuụi t bt k loi bụng thụng thng no Cỏc thụng tin chi tit hn, xem phn V.1 bỏo cỏo ỏnh giỏ ri ro Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt ỏnh giỏ kh nng gõy c v gõy d ng sn phm ca gen chuyờn gõy S tng ng c bn ó c chng minh cho bụng chuyn gen GHB614 Ht t bụng chuyn gen ch cú s khỏc bit v thnh phn protein mi l 5- enolpyruvylshikimate-3phosphate synthase (2mEPSPS) so vi i chng khụng chuyn gen õy l protein khụng mn cm vi glyphosate, v cú hot tớnh enzyme to tớnh khỏng thuc tr c (Van der Klis et al., 2006) Enzyme tng hp EPSP (EC.2.5.1.19) cú mt ph bin t nhiờn v vi hn 30 nm nghiờn cu, cu trỳc v chc nng ca nú ó c xem xột v hiu rừ gn nh hon chnh õy l enzyme th v l enzyme gn cui cựng ng chuyn hoỏ shikimate sinh tng hp cỏc sn phm mch vũng c tỡm thy vi sinh vt v cõy trng (Herrmann, 1995; Steinrỹcken v Amrhein, 1980) Phn ng c xỳc tỏc bi enzyme ny l phn ng chuyn hp phn enolpyruvyl ca phosphoenol pyruvate (PEP) ti 5-hydroxyl of shikimate-3-phosphate (S3P), to sn phm 5-enolpyruvyl-3-shikimate phosphate (EPSP) Trong cõy trng thụng thng, EPSPS b c ch chn lc bi glyphosate lm thiu cỏc amino thm, kt qu l lm cht cõy Gen 2mepsps c chốn vo ó c gii trỡnh t hon ton v mó hoỏ cho protein 47kDa gm 445 axit amin Gen bin i to protein cú 99,5 % ging so vi protein t ngụ Protein bin i ny tng ng cao so vi cỏc enzyme EPSPS khỏc c tỡm thy cỏc loi cõy trng ó c s dng lm thc phm mt cỏch an ton lõu i (go, lỳa mỡ, nho, v c chua) Khụng cú bỏo cỏo no v c tớnh v tớnh di ng ca protein enzyme EPSPS S cú mt protein 2mEPSPS chui thc phm v thc n chn nuụi khụng phi l mi v cng khụng cú nguy c xy ri ro Protein EPSPS cú vai trũ sinh hoỏ c bit v c bit n rt rừ cõy trng Cỏc c im hoỏ sinh ca enzyme 2mEPSPS ó c xỏc nh chi tit s so sỏnh vi protein t nhiờn wtEPSPS Tr tớnh khỏng li glyphosate, s khỏc bit v axit amin khụng to nhng khỏc bit v c im sinh hoỏ Cỏc nh hng ti trao i cht ca 2mEPSPS cõy trng ging nh cỏc protein EPSPS ni sinh khỏc tr tớnh chng chu vi glyphosate Protein 2mEPSPS cng cú mt s kin ngụ khỏng glyphosate GA21 (mó nhn din MON-ỉỉỉ21-9) ó c cp chng nhn an ton cho canh tỏc v s dng lm thc phm/thc n chn nuụi nhiu vựng lónh th khỏc Kt lun v kh nng gõy c ca thnh phn mi: Bayer CropScience cho rng cú bng chng khng nh protein 2mEPSPS an ton cho ngi v vt nuụi Cỏch thc ỏnh giỏ tớnh an ton ca mt protein mi l so sỏnh cỏc c im ca nú vi cỏc cht gõy d ng thụng thng thc phm Mt cỏch khỏc khỏc l so sỏnh s tng ng v trỡnh t vi trỡnh t cỏc cht gõy d ng ó bit Bi hu ht cỏc cht gõy d ng u bn mụi trng axit d dy v cỏc protease phõn gii h tiờu hoỏ, ng thi bn vi iu kin ch bin (nh nhit cao), vy bn ca protein 2mEPSPS di cỏc iu kin trờn cng c ỏnh giỏ Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt Cỏc kt qu trờn khng nh s an ton ca protein 2mEPSPS i vi ngi v ng vt bi vi s phõn gii nhanh chúng, nh vy, c hi protein ny tr thnh cht gõy c v gõy d ng l khụng cú Quỏ trỡnh ch bin s dng nhit, chng hn nu, x lý hi ỏp sut cao, x lý dung mụi, kim s phõn gii protein 2mEPSPS protein Trong khụ du bụng khụng sy, protein 2mEPSPS tn ti, nhng sn phm khụ sy, du thụ hay du tinh ch, nú khụng th phỏt hin c, hay c vi si bụng di v x ngn cng khụng th phỏt hin c protein ny (Currier, 2007 ) DNA thng khụng cú mt du thc phm hay x ngn bụng sau ó tri qua quỏ trỡnh ch bin k cng BSC tin hnh cỏc nghiờn cu v khng nh s tng ng v thnh phn ca bụng chuyn gen vi i chng khụng chuyn gen ca nú Coker312 Thờm vo ú, cỏc d liu t ht bụng ó ch bin nh ht tỏch si di, t, v ht, khụ du bụng sy hoc khụng sy v t du thụ cng nh du tinh t bụng chuyn gen GHB614 v i chng khụng chuyn gen c ỏnh giỏ v ch s tng ng cú ý ngha Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt PHN VI XUT CC BIN PHP QUN Lí RI RO CA THC VT BIN I GEN I VI SC KHE CON NGI V VT NUễI Bayer CropScience ó tin hnh ỏnh giỏ an ton ca thc vt bin i gen v thc phm, thc n chn nuụi cú ngun gc t bụng GHB614 Cỏc nghiờn cu v bỏo cỏo cho n khụng xỏc nh c bt k nguy c tim n no vi bụng chuyn gen mang s kin GHB614 Kt lun v ỏnh giỏ ri ro i vi bụng mang s kin GHB614 l an ton v cú thnh phn tng ng vi i chng truyn thng v cỏc ging bụng thng mi K hoch giỏm sỏt xut a vic giỏm sỏt chung cỏc nh hng bt li cú th cú, lp tc hay chm tr, trc tip hay giỏn tip ca bụng chuyn gen GHB614 n sc kho ca ngi v mụi trng PHN VII KT LUN V KIN NGH Khụng cú ri ro no liờn quan n bụng chuyn gen GHB614 c tỡm thy sau phõn tớch v ỏnh giỏ i chng truyn thng c s dng phõn tớch so sỏnh Vic thay i di truyn cú ch ớch l tng li ớch v mt nụng hc, khụng lm thay i cỏc thnh phn dinh dng hay giỏ tr ca chỳng Khụng cú quan ngi cú ch ớch no liờn quan n sc kho ca ngi Thc phm v thc n chn nuụi cú ngun gc t GHB614 s khụng thay th hay lm thay i thc phm v thc n chn nuụi truyn thng GHB614 khụng mang nhng c im c bit no lm tng ch dinh dng so sỏnh vi bụng truyn thng Khụng cú bng chng no cho thy nh hng v lõu di i vi dinh dng v sc kho vi nhng sn phm thc phm cú ngun gc t GHB614 Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe ngi v ng vt TI LIU THAM KHO AGBIOS 2006 AGBIOS Database Product Description MON-ỉỉỉ21-9 (GA21) pages http://www.agbios.com/static/cropdb/LONG_GA21_printer.html M-278133-01-1 Bolivar F; 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Cunny H; Herouet-Guicheney, 2006 Safety assessment of the double mutant 5enol- Pyruvylshikimate-3-phosphate synthase (2mepsps) protein, Bayer CropScience S.A., Sophia Antipolis, France, 43 pages, M-278169-01 -1 Zhu J; Oger P M; Schrammeijer B; Hooykaas P J J; Farrand S K; Winans S C, 2000 The bases of crown gall tumorigenesis, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Cornell University, Ithaca, NY; Institute of Molecular Plant Sciences, Leiden, Netherland, 11 pages, M-290743-01-1 [...]... tinh t bụng chuyn gen GHB614 v i chng khụng chuyn gen c ỏnh giỏ v ch ra s tng ng cú ý ngha Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con ngi v ng vt PHN VI XUT CC BIN PHP QUN Lí RI RO CA THC VT BIN I GEN I VI SC KHE CON NGI V VT NUễI Bayer CropScience ó tin hnh ỏnh giỏ an ton ca thc vt bin i gen v thc phm, thc n chn nuụi cú ngun gc t bụng GHB614 Cỏc nghiờn cu v bỏo cỏo cho n nay khụng... con ngi v vt nuụi Cỏch thc ỏnh giỏ tớnh an ton ca mt protein mi l so sỏnh cỏc c im ca nú vi cỏc cht gõy d ng thụng thng trong thc phm Mt cỏch khỏc khỏc l so sỏnh s tng ng v trỡnh t vi trỡnh t cỏc cht gõy d ng ó bit Bi hu ht cỏc cht gõy d ng u bn trong mụi trng axit d dy v cỏc protease phõn gii trong h tiờu hoỏ, ng thi bn vi iu kin ch bin (nh nhit cao), do vy bn ca protein 2mEPSPS di cỏc iu kin trờn...Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con ngi v ng vt PHN IV THễNG TIN V THC VT BIN I GEN Mụ ta qua trinh tao ra thc vt biờn ụi gen Quỏ trỡnh chuyn gen on ADN t plasmid pTEM2 c chuyn vo h gen ca bụng GHB614 bng vi khun Agrobacterium Vi phng phỏp chuyn gen ny, on ADN nm gia b phi v b trỏi ca T-DNA t plasmid pTEM2 c chuyn lờn h gen ca cõy trng Cỏc vựng b phi v trỏi... lm thiu cỏc amino thm, kt qu l lm cht cõy Gen 2mepsps c chốn vo ó c gii trỡnh t hon ton v mó hoỏ cho protein 47kDa gm 445 axit amin Gen bin i to ra protein cú 99,5 % ging so vi protein t ngụ Protein bin i ny tng ng cao so vi cỏc enzyme EPSPS khỏc c tỡm thy trong cỏc loi cõy trng ó c s dng lm thc phm mt cỏch an ton lõu i (go, lỳa mỡ, nho, v c chua) Khụng cú bỏo cỏo no v c tớnh v tớnh di ng ca protein... ca gen c chốn vo ti giỏ trnh dinh dng ca ht bụng, du, khụ v v ht bụng Vic thờm gen mi vo h gen ca bụng khụng lm thay i thnh phn v cht lng dinh dng Trờn phng din dinh dng, thc phm v thc n chn nuụi t bụng GHB614 ging vi thc phm v thc n chn nuụi t bt k loi bụng thụng thng no Cỏc thụng tin chi tit hn, xem phn V.1 trong bỏo cỏo ỏnh giỏ ri ro Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con. .. trong bng 2 cng c chốn vo h gen bụng s kin Glytol GHB614 Mc dự mt s gen s dng trong quỏ trỡnh chuyn gen cú xut x t A tumefaciens, vn c bit l vi sinh vt gõy bnh cho cõy, to ra khi u cho cõy c loi b v khụng hp nht vo h gen cõy nhn (Deblaere v cng s, 1985) Gen 2mepsps c s dng trong mt s cõy trng nụng nghip to ra kh nng khỏng thuc tr c glyphosate cho cõy trng Cỏc thụng tin chi tit v quỏ trỡnh chuyn gen, ... ớch trong trỡnh t, nú khụng b c ch bi glyphosate v s biu hin ca nú to ra hot tớnh m bo s khỏng li thuc tr c glyphosate cho bụng GHB614 Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con ngi v ng vt S thay i kiu hỡnh tng ng vi s chuyn gen ú l bụng GHB614 phỏt trin tt khi s dng thuc tr c glyphosate Tớnh trng ny khụng nh hng ti mụi trng vụ sinh cng nh hu sinh xung quanh Thụng qua cỏc nghiờn... Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con ngi v ng vt Cỏc kt qu trờn khng nh s an ton ca protein 2mEPSPS i vi con ngi v ng vt bi vi s phõn gii nhanh chúng, nh vy, c hi protein ny tr thnh cht gõy c v gõy d ng l khụng cú Quỏ trỡnh ch bin s dng nhit, chng hn nu, x lý hi ỏp sut cao, x lý dung mụi, kim s phõn gii protein 2mEPSPS protein Trong khụ du bụng khụng sy, protein 2mEPSPS... Agropecuario Túm tt bỏo cỏo ỏnh giỏ ri ro bụng mang s kin GHB614 ti sc khe con ngi v ng vt PHN V NH GI NGUY C NH HNG CA THC VT BIN I GEN I VI SC KHE CON NGI V VT NUễI Ngoi tớnh trng khỏng glyphosate c a vo cú ch ớch, cú th thy khụng cú bt k thay i no khỏc hoc cú thay i xut hin mt cỏch khụng ch ớch v thnh phn ca bụng GHB614 so vi i chng khụng chuyn gen Coker 312 Cỏc d liu v axit bộo, proximate, axit amin,... bụng chuyn gen mang s kin GHB614 Kt lun v ỏnh giỏ ri ro i vi bụng mang s kin GHB614 l an ton v cú thnh phn tng ng vi i chng truyn thng v cỏc ging bụng thng mi K hoch giỏm sỏt xut a vic giỏm sỏt chung cỏc nh hng bt li cú th cú, lp tc hay chm tr, trc tip hay giỏn tip ca bụng chuyn gen GHB614 n sc kho ca con ngi v mụi trng PHN VII KT LUN V KIN NGH Khụng cú ri ro no liờn quan n bụng chuyn gen GHB614 c