Giáo trình ký sinh trùng học thú y (giáo trình dành cho bậc cao học) phần 2

Chitcmg M T SO CHUYfcN DE BENH GIUN SAN GIA SUC BENH SAN LA GAN TRAU, BO, DE (Fasciolosis) Benh san la gan d trau, bo, de hai loai san la ky sinh d ong dan mat va gan gay {Fasciola hepatica va Fasciola gigantica) Ngoai trau, bo, de, hai loai san gay benh cho cac dong vat nhai lai khac, doi thay ca d ngudi F hepatica va F giantica la hai loai san la ph6 bien d cac vung thuoc chau A va Chau Phi Theo Stemphenson (1947) va Urquhat (1956), tac hai cua san la gan doi vdi gia sue nhai lai rat Ion, bidu hien r5 nhat la gay thieu mau, viem va xo gan gia sue bi nhidm san la gan d muc nang DAC DIEM SINH HOC CUA SAN LA FASCIOLA 1.1 Vi tri ciia san la Fasciola he thong phan loai dong vat hoc Theo Skrjabin va cs (1977), Nguydn Thi Le va cs (1996), san la gan ky sinh va gay benh cho gia sue nhai lai dugc xep he thong phan loai dong vat nhu sau: Nganh Plathelminthes Schneider, 1873 Phan nganh Platodes Leuckart, 1854 Lop Trematoda Rudolphi, 1808 Phan lop Prosostomadidea Skrjabin va Guschanskaja, 1962 Bo Fasciolida Skrjabin et Schulz, 1937 Phan bo Fasciolata Skrjabin et Schulz, 1937 Ho Fasciolidae Railliet, 1895 Phan ho Fasciolinae Stiles et Hassall, 1898 Giong Fasciola Linnaeus, 1758 Loai Fasciola hepatica (Linnaeus, 1758) Loai Fasciola gigantica (Cobbold, 1885) 1.2 Dac diem hinh thai cua san la Fasciola Cung nhu nhieu loai san la khac, san la gan luong ti'nh, co the thu tinh cheo hoac tu thu tinh San co giac mieng va giac bung, giac bung khong noi voi co quan tidu hoa San khong co he ho hap, tuan hoan va co quan thi giac (d giai doan mao au co dau vet sSc to mat) He sinh due rat phat trien voi ca bo phan sinh due due va cai cung mot san Tu cung san chua day trung Co thd phan biet hai loai san la gan thuoc giong Fasciola nhu sau: F gigantica (Linnaeus, 1758): co chieu dai than gap lan chieu rong, “vai” khong co hoac nhin khong ro ret, nhanh ruot chia toa nhieu nhanh ngang 123 F gigantica (nghla la san la “khong lo”) dai 25 - 75 mm, rdng - mm, u 16i hinh non cua d£u la phzin tiep theo cua than, vi vay no khdng c “vai” nhu loai khac cua gidng Fasciola Hai ria ben than san song song vdi nhau, d£u cudi cua than tu Giic bung tron ldi Rudt, tuyen noan hoang, buong trung va tinh hoan ddu phan nhanh Trung hinh bau due, mau vang nau, phdi bao to ddu va xep km vo Kfch thudc trung: 0,125 - 0,170 x 0,06 - 0,10 mm F hepatica (Linnaeus, 1758): trai vdi loai trfen, loai than rdng, dau ldi va nhd phia trudc lam cho san co “vai”, nhanh ruot chia it nhanh ngang hon F hepatica (nghla la san la d gan) dai 18 - 51 mm, rdng - mm, phin trudc than nhd ra, tao cho san co vai be hai ben Hai ria ben than san khdng song song vdi ma phinh d chd vai roi thot lai d doan cudi than Nhiing dng dan tuyen noan hoang chay ngang, chia vung giua cua san phan trudc va ph£n sau than Ph£n sau than co tinh hoan va bo phan sinh due due Tinh hoan phan nhidu nhanh xep phia sau than Tu cung d phctn giua than trudc tao nen mot mang ludi rd'i nhu to vo Budng trung phan nhanh nam d sau tu cung Trumg san cd hinh thai, mau sac tucmg tu trung cua loai F gigantica, kich thudc 0,13 - 0,145 x 0,07 - 0,09 mm F hepatica F gigantica F gigantica Hinh 44 San la Fasciola 124 Tri'cng Fasciola 1.3 Chu ky sinh hoc cua san la Fasciola Chu ky sinh hoc cua san la Fasciola da duoc Leukart (1882) nghien curu d Dure va Thomas (1882) nghidn curu d Anh Fasciola trucmg ky sinh dng dan mat cua trau, bo, de Sau thu tinh, m6 i san de hang chuc van trumg Nhung trumg cung dich mat vao ruot, sau theo phan ngoai Neu gap didu kien thuan loi: duoc nude mua cud'n troi xuong cac vung nude, ho, ao, sud'i, ruong nude nhiet 15 - 30°C, pH = - 7,7, co anh sang thfch hop sau 10 - 25 trumg nd Miracidium boi tir nude Neu thieu anh sang, Miracidium khong co kha nang thoat vo nhung van ton tai den thang vo, Miracidium co hinh tam giac, xung quanh than co long va di chuyen duoc nuoc Khi gap vat chu trung gian thfch hop (oc Lymnaea), Miracidium xam nhap co thd oc va phat trien bao a'u (Sporocyst) Nhung Miracidium khong gap vat chu trung gian thi runglong, rtfa dan va chet Bao a'u (Sporocyst) hinh tui, mau sang, duoc bao boc bdi ldp mang mdng, cac te bao ngon lua hoat dong hinh hau, ong ruot va cac dam phdi Trong oc cd the cd - au trung Khoang - ngay, bao au sinh san vo tfnh cho nhidu Redia (loi a'u) Mot bao a'u sinh - 15 ldi a'u Redia hinh suot chi, ft hoat dong, cd mieng, hau, ruot, hinh tui don gian Cd hai he: Redia the he I va Redia the' he II cung phat tridn oc - vat chu trung gian nhiet 16°C hoac tha'p hon, ldi a'u chi san sinh Redia I va dung phat trien d nhiet phu hop (20 - 30°C), sau 29 - 35 ngay, ldi au bien vi a'u (Cercaria) Mot Redia cd the sinh -2 Cercaria Cercaria (vi au) la au trung d pha sdng tu cua san la gan, cd cau tao than hinh trdn lech, dudi dai hom than giup vi a'u van chuyen duoc dd dang nude Ca'u tao cua vi a'u gom giac mieng, giac bung, hau, thuc quan va ruot phan hai nhanh Theo Ginyecisz - Kaija (1960), co the Cercaria cdn cd nhung hat Glycogen cung cap nang lucmg cho hoat dong song ciia au triing, dac biet la cho su van dong khdng ngimg cua dudi Dudi la co quan van dong ciia vi a'u Theo mot sd tac gia, dudi lam nhiem vu thay doi vi tri ciia au triing mdi trudng nude Lizz (1922) cho biet, nhd sir hoat dong tfch cuc cua dudi ma vi au tid'p can dd bam vao cac cay thuy sinh, tao ken (Adolescaria) Tir Miracidium chui vao oc den phat trien Cercaria can khoang 50 80 Sau thuc, Cercaria thoat khoi oc, mdi trudng ngoai, boi tu nude, cd kfch thudc 0,28 - 0,30 mm chieu dai va 0.23 mm chieu rong Sau vai gid boi nude, Cercaria rung dudi, tiet chat nhay xung quanh than, chat nhay gap khdng khf kho rat nhanh Luc Cercaria da bien Adolescaria 125 Hinh 45 Cdc dang du triing ciia san Fasciola 1a Mao au nc) tir trimg san; 1b Bao au 2a Loi au non; 2b Loi au gia co vT au non 3a Loi au me phong nhieu loi au 3b V7 au au triing san la gan chui khoi oc 4a V7 au rung duoi tao ken 4b Ken san la gan Adolescaria hinh khoi tron, ben chura phoi hoat dong Phoi co giac mieng, giac bung, ruot phan nhanh va tui bai tiet Adolescaria thudng d nude hoac bam vao cay cd thuy sinh Neu trau, bd, de nuot phai Adolescaria, vao den da day va ruot ldp vo ngoai bi phan huy, au trung duoc giai phong va di chuyen den dng mat bang dudng: - Mot sd a'u trung dung tuyen xuyen chui qua niem mac ruot, vao tmh mach ruot qua tinh mach cua vao gan, xuyen qua nhu mo vao dng mat - Mot sd a'u trung khac cung dung tuyen xuyen chui qua ruot vao xoang bung, den gan, xuyen qua vd gan vao dng mat 126 - Mdt sd a'u trung tir ta trang ngugc dong dich mat d6 len ong dan mat Sau vao ong dan mat, a'u trung ky sinh d va phat trien san la gan trudng Theo Skerman (1966), thdi gian hoan vong ddi la 92 - 117 Fasciola trudng cd the ky sinh dng dan mat cua sue vat nhai lai - nam, cd tdi 11 nam Hinh 46 Vdng dai phat trien cua san Fasciola Trung nd Miracidium nude Miracidium vao oc - vat chu trung gian Cercaria rdi khoi oc Adolescaria bam tren cay co thuy sinh Sue vat nhai lai nuot Adolescaria San la gan tri/dng ky sinh ong dan mat I Theo Phan Dich Lan (2004), khoa hoc thu y nude ta da nghien cuu cong vdng ddi cua san la gan Trong dieu kien nhiet thfch hop (28 - 30°C), cd oc vat chu trung gian (Lymnae swinhoei va Lymnae viridis), cd vat chu cudi cung (trau, bd, de, ciru) thi vdng ddi cua san la gan d nude ta duoc xac dinh vdi cac mure thdi gian sau: - O ngoai thien nhien: trung san la gan nd mao a'u (Miracidium ) khoang - - O oc vat chu trung gian: Mao a'u (Miracidium ) phat trien bao au (Sporocyst) can Bao a'u (Sporocyst) phat trien ldi a'u (Redia) can - Ldi a'u (Redia) phat trien vT au (Cercaria ) non can - ngay, v! au trudng can - ngav - ngoai oc vat chu trung gian: vT a'u phat trien ken (Adolescaria) sau gid - trau, bd: trau bd, be nghe nuot phai Adolescaria , sau 79 - 88 dng dan mat cua trau bd da cd san la gan trudng de trung theo phan nsoai 127 Dieu kien nhiet dd va am cua nuoc ta ra't thuan lpi cho su nhiem va gay benh cua san la gan (ke ca gay nhiem va nhiem tu nhien) C) nhung vung co mam benh ton tai, cu trung binh thang san la gan lai hoan vong ddi co the trau, bo, nghla la trau bo lai tao mot ddi san mdi Con vat da cd san la gan ky sinh lai tiep tuc nhidm them mam benh mdi, gay tinh trang b6 i nhiem san la gan vi vay cudng nhifem tang len theo tuoi trau, bd DAC D IEM D ICH T £ HO C BENH SAN LA FASCIOLA Benh san la gan bien d khap cac chau luc va nhieu nude tren the gidi Viet Nam, benh tha'y d khap cac tinh ca nude Houmeder (1938) da dieu tra va tha'y, trau, bd, de, ciru, thd d mien Bac Viet Nam deu nhiem san la F gigantica theo ty le thur tu la 64,7%; 23,5%; 37%; 52,94%; 14,28%; dac biet cd trudng hop ngudi nhidm san Trinh Van Thinh (1978) cho bie't, trau trudng mac benh san la gan F gigantica, ty le nhiem tdi 50 - 70% Theo Phan Dich Lan (1980), mo kham 1043 trau d Thai Nguyen, sd trau nhiem san la gan la 57%, dd cd nhieu gan phai huy bd sd luong san qua nhieu Ket qua dieu tra d huyen Binh Luc - Nam Ha, ty le nhidm san la gan d trau la 51,2 - 57,5% Doan Van Phuc (1980) da kiem tra 64 bd tai trai bd sua Ha Noi, tha'y ty le nhidm san Fasciola la 73,43% Tac gia cdn cho biet, benh san la gan da anh hdng rd ret den sUc khoe va san luong sua cua dan bd Nguydn Due Duong (1995) da xet nghiem phan cua 537 huou tai cac trai nuoi huou d Ha Tinh, Quynh Luu, Didn Chau (Nghe An) va cong vien thu le Ha Noi, phat hien 13,22% sd huou nhidm san F gigantica Theo Nguydn Quang Sue va Nguydn The Hung (1995), dan dd Bach Thao sau hon nam nudi tai Trung tam nghidn cuu dd, thd Son Tay va mot sd gia dinh nuoi de nhidm san la gan vdi ty le 30,4% Doan Van Phuc, Vuong Due Chat, Duong Thanh Ha (1995) cho bie't, trau bd thuoc khu vuc Ha Noi nhidm san la gan ty le 53,41% Ket qua kiem tra trau, bd d mot sd dia phuong xung quanh Ha Noi, Bac Giang Thai Nguydn, Hoa Binh, ty le nhidm san la gan la 44,53% Trong dd, trau nhidm 33.92% bd nhidm 54,21% (Luong Td Thu va Bui Khanh Linh, 1996) Nguyen Thi Kim Lan (2000) da nghidn cuu va cho biet, dan dd dia phuons nuoi d cac tinh Thai Nguydn, Bac Kan, Tuyen Quang va Cao Bang nhidm san la gan bien dong tir 5,3% den 27,9% theo dia phuong Ty le va cudng nhidm san la gan d gia sue nhai lai phu thuoc vao nhung veu td sau: - Yeu td thdi tiet, hau va mua vu 128 Thai tiet, hau cua mot vung, mot khu vuc co lien quan true tiep den su ton tai va phat trien cua oc - vat chu trung gian cua san la gan Dieu kien am udt, mua nhidu tao moi truong nude, giup oc nude ngot song va sinh san thuan loi Trinh Van Thinh (1963), Trinh Van Thinh va cs (1978), Pham Van Khue va Phan Luc (1996), Nguydn Thi Kim Lan va cs (1999) ddu cho biet, gia sue nhai lai nhidm san la gan thudng tang ldn vao mua vat chu trung gian phat trien Nhung nam mua nhieu, ty le nhidm san la gan tang len so vdi nhirng nam nang rao va kho han Mua vu gan lidn vdi su thay doi thdi tiet khf hau Miia he thu, sd gia sue bi nhidm san la gan tang cao hon cac miia khac nam Cudi miia thu va miia dong benh thudng phat - Yeu td viing va dia hinh Viing va dia hinh la hai khai niem khac nhau, song cd lidn quan chat che vdi Cac viing khac cd dia hinh khong gidng Dia hinh la yeu td quan quyet dinh su khac giua cac vung Cac viing khac trdn the gidi ndi chung va d Viet Nam ndi rieng deu thuoc bon loai dia hinh: ven bien, ddng bang, trung du va mien nui Hau het cac nha ky sinh triing hoc thong nhat rang, gia sue nhai lai d viing ddng bang nhidm san la gan nhieu nhat, ty le va cudng nhidm giam dan ddi vdi dan gia sue nhai lai d viing ven bie’n, vung trung du va viing nui Ve nguydn nhan dan den quy luat nay, cac tac gia (Trinh Van Thinh, 1963; Pham Van Khue va cs, 1996; Soulsby, 1982; Kaufmann, 1996 ) deu giai thfch: viing ddng bang cd nhieu hd, ao, kenh, rach, cd dieu kien cho oc - vat chu trung gian sdng va sinh san Cac kieu dia hinh khac thi van dd han che hon so vdi ddng bang Hinh 47 Dan bd chan thd d bdi chan cd nguy ca nhiem san Fasciola Phan Dich Lan (1994) da dieu tra 7.359 trau, bd d qua thay: trau bd d vung ddng bang nhidm san la gan du, viing ven bidn va mien nui (binh quan ty le nhidm viing ddng bang tir 19,6% den 61,3%, viing trung du tir 26 tinh mien Bac Viet Nam, ket cao nhat, sau dd den vung trung san d cac viing dieu tra nhu sau: 16,4% den 50,2%, viing ven bien 129 tir 13,7% den 39,6%, vung nui tCr 14,7% d£n 44,0%) Theo Nguydn Dang Khai (1996), ty Id nhidm san la gan trau bo vung ddng bang qua m kham la 88 %, vung trung du lk 77,6% - Yeu td loai va tudi vat chu cudi cung Sue vat nhai lai da duoc thuan hoa nhu trau, bo, de, ciru ddu nhidm san la gan Fasciola Ngoai ra, sue vat hoang da cung nhidm san la (hucru, nai, hoang ) Cung co nhiing trudng hop tho, ngua, Ion nhiem Fasciola, ca ngudi cung co the nhiem san nude ta, theo Trinh Van Thinh va cs (1978), Pham Van Khue va cs (1996), loai sue vat nhidm san la Fasciola nhieu nhat la trau (79,6%), bo it hon (36%), dd ft nhat (20%) So di trau nhidm san la gan nhidu la dac tfnh ua nude cua chung (thfch an g£n ch6 co nude, dam tam nude va udng nude vung, ao, kenh rach), dac diem cua bd va dd ft ua nude hon Theo Nguydn Thi Kim Lan va cs (1998), dd dia phuong Thai Nguydn, Bac Kan, Tuyen Quang va Cao Bang nhidm san la gan d cac lura tu 6i la 14,88%, d dd truong la 22,86% Tac gia nhan xet: thieu thufc an, ca dan dd cd th£ loi xuong ruong nude d£ an co sdng dudi nude, dd dd dang nuot phai ken gay bdnh Vd mdi lidn quan giua ty le, cudng nhidm san la gan va tuoi v |t chu, cac tac gia deu thong nhat rang, tuoi sue vat cang cao thi ty le va cudng nhidm cang tang ldn Mot dieu dd nhan tha'y la, sue vat tuoi cang tang ldn (thdi gian so'ng cang dai) thi su tid'p xuc vdi mdi trudng ngoai canh cang nhieu, co hoi gap va nuot phai nang a'u (Adolescaria) cang cao Mat khac, san Fasciola truong cd thdi gian ky sinh d sue vat nhai lai tuong doi dai ( - nam, tham chf tdi 11 nam) Dd chfnh la cd sd khoa hoc giai thfch cho quy luat nhidm theo tuoi vat chu cua san la Fasciola sp Phan Dich Lan (1994, 2004) cho bie't, trau dudi nam tuoi chi nhidm san la gan 17,2 - 22,0%; trau - nam tuoi nhidm san la gan 31,2 - 40,2%, trau - nam tuoi nhidm 42,4 - 57,5%, trau trdn nam tuoi nhidm 56,8 - 66,3%, trau o tuoi phe' canh (loai thai) mo kham thay ty le nhidm cao tdi 84,6% (nhung trau bi benh ra't nang, gan phai huy bo toan bo cd qua nhieu san ky sinh) Theo Nguydn Thi Kim Lan (2000 ), ty le va cudng nhidm san la gan cung tang ldn theo tuoi dd Nguon gieo rac benh chu yeu la sue vat nudi (trau, bd, dd, ciru ) va nhung da thu mang Fasciola Trung san la gan theo phan cua sue vat ngoai tu nhidn Hang nam, mdi san la gan ky sinh de khoang 6000 trung Vi vay, mdi sue vat mang san hang nam thai khoi luong trung kha ldn ddng co va cac bai chan tha Nhung dong co am thap, lay loi la nhung noi c£n thiet de mam benh phat trien va xam nhap vao sue vat, dong thdi thuan loi cho trirng no Miracidium va thuan loi cho vat chu trung gian cua Fasciola ton tai va phat trien - Vat chu trung gian cua Fasciola sp Su phan bo cac loai oc - vat chu trung gian cua san la gan phu thuoc vao cac \iing dia ly khac Theo Pham Van Khue va cs (1996), Kaufmann (1996), vat chu trung 130 gian cua san la Fasciola la cac loai 6'c nude ngot ho Lymnaea: L auricularia, L swinhoei, L viridis, Galba truncatula, Radix ovata Phan Dich Lan (1980) da tong hop va cho biet: loai oc - vat chu trung gian cua F A/ gigantica b An Do la L acuminata, b chau Phi la L natalensis, b Pakistan la L permisca, b Apganixtan la L luteola, b Malaixia la L auricularia, b Nhat Ban la L pervia, b Indonexia va Philippine la L viridis, b Hungari la G truncatula v.v A/ Oc G truncatula la loai oc nho, dai 10 mm, rong mm, vo mau xam; dau dai, co gai thit Nhiet tot nhat d£ loai oc phat trien la 20 - 22°C, pH: 6,6 - 8,6 , sinh san manh tir thang den thang Theo Jorgen Hansen va cs (1994), nhiet thfch hgp nhat cho su phat trien cua oc la 15 - 26°C, b nhiet chung de so lugng trung ra't Ion Trung no vong tuan va sau thang oc trudng Mot 6c vong -1 tuan co the sinh san hang ngan oc O dudi 10°C, 6c khong phat trien va ngimg hoat dong Tuy nhien, 6c co the chui vao bun va song dieu kien ba't lgi nhieu thang Phan Dich Lan va cs (1985) da khao sat dac diem sinh hoc cua oc - vat chii trung gian ciia F gigantica b mien Bac Viet Nam Tac gia cho biet, vat chii trung gian ciia san la Fasciola la hai loai oc nude ngot thuoc giong Lymnae vdi ten goi la oc vanh tai (L swinhoei) va oc chanh (L viridis) Loai L swinhoei co vo mong, dd vd, khong co nap mieng, kfch thudc 20 mm, vong xoan cuoi ciing rat ldn, chiem gan het phan than, vo loe nhu vanh tai Loai L viridis cung co vo mong, khong co nap mieng, kfch thudc 10 mm, vd d£ vd, co - vong xoan, vong xoan cuoi cung ldn (hinh 48) Hinh 48 1,3 L viridis a' O c k y c h it tr u n g g ia n 2,4 L swinhoei G truncatula 131 A/ / Oc L viridis thucmg sdng nhung noi nude xam x 2fp, de trimg - trung, sau no 6c Oc L swinhoei thucmg s6ng trfti n i d c 6ng ranh, ao, h , de trumg quanh nam, m i co 60 - 150 trung Trong didu kidn nhiet d d nude ta, 6c de quanh nam va quanh nam co 6'c dupe nd Phan Dich Lan (1994, 2004) cho bidt: 6c L swinhoei phan b6 nhidu hem d vung dong bang, 6c L viridis phan bo nhidu hom d vung nui, trung du va ven bi&i Theo ket qua nghien cun cua tac gia thi hai loai 6c ddu xua't hidn ca 12 thang ciia nam, nhung mat (tmh tren m2) khac theo vung: viing d 6ng bang, mat dd 6c L swinhoei cao hem va phan bo ddu nam, oc L viridis thi xua't hien vdi mat d6 cao hem d cac viing nui, trung du, ven bien Tir do, tac gia nhan xet rang, L swinhoei chiu nude hom, L viridis chiu can hon Su t6n tai va phat trien quanh nam, d ta't ca cac vimg cua 6c - vat chu trung gian la didu kien quan nhat lam cho ty le nhidm san la gan ciia sue vat nhai lai d nude ta cao va bien d tat ca cac viing Phan Djch Lan (1994, 2004) da tong hop va cho biet, nude ta dupe xep vao mot nude d chau A lua nude cd dan trau, bd nhidm benh san la gan vdi ty le cao nhat Ket qua dieu tra d mot so viing cho tha'y: l l tinh midn nui, ty le nhidm san la gan cua trau bo la 39% CJ tinh trung du, ty le nhidm san la gan ciia trau bo la 42,2% O tinh thuoc viing dong bang, ty le nhidm san la gan la 57,5% O tinh thuoc viing ven bien, ty le nhidm san la gan la 36,7% Theo Nguydn Trong Kim (1997), giua ty Id nhidm a'u triing san la gan cua 6c - vat chu trung gian vdi ty le nhidm san la gan cua trau, bo co mdi tuong quan thuan, nghla la, ne'u ty le nhidm a'u triing san la gan d 6c nude ngot cao thi ty le nhidm san la gan cua trau, bo d khu vuc cung cao - Sure de khang cua trumg va a'u triing san la gan Trumg san la gan dupe thai theo phan sue vat nhai lai m i trudng ngoai canh Trumg san ra't nhay cam vdi dieu kien kho han va tac dong true tiep ciia anh sang mat trdi O phan kho, phoi ngimg phat trie’n, trumg bi chet sau - Trong dieu kien kho han, vd trung bi nhan nheo, bien doi hinh dang, Miracidium bi chet vo trung sau -1 ,5 moi trudng 3m udt, trumg cd kha nang song kha lau (trong phan hai am, trung ton tai den thang) Dudi anh nang chi£u true tiep, trumg chet nhanh Phoi bi chet sau d nhiet thap (-5°C -> - 15°C) Nhiet 10 - 20° C, trung ngimg phat trien Nhiet 40 - 50°C, phoi chet sau vai phut (Pham Van Khue va cs, 1996) Khi phat trien den giai doan nang au (Adolescaria), sue dd khang ciia chung tang len ro ret Adolescaria co kha nang ton tai d nhiet -4°C -> -6°C dieu kien nhiet 132 D A C D I ^ M B $ N H L t V A L A M S A N G B fe N H C A U T R U N G L O N 3.1 D dc d ie m benh ly Theo Kolapxki N.A va cs (1980), mang nidm mac rudt, cdu triing phat tridn manh bang sinh san vd tinh va lam cho hang loat td bao bidu bi bi chdt Ngudi ta xac dinh rdng, mdt vdt mdc bdnh cdu trung thai mdi trudng bdn ngoai hang til tridu ddn 980 trieu nang triing Didu co nghla la co thd vdt 6m, hang bi chdt trdn 500 tridu t£ bao bidu bi rudt Khdng nhung chi cdc td bao cdu trung sinh san manh me, ma hinh nhu ca nhung td bao bdn canh, nhiing mao mach va mach quan bi pha huy Su pha huy hang loat cdc td bao cua ky chu lam cho tinh toan ven cua vach rudt bj tdn thuong Nhung vung rudt bi phd huy se bi vi sinh vdt xdm nhdp vdo lam phuc tap them cho qua trinh sinh bdnh va gdy nhiing huy hoai ldn cho mang nidm mac Vi vdy, nhieu doan rudt khdng tham gia dupe vao qua trinh tidu hoa Didu lam cho vdt doi dai dang, ddn tdi su ngung dong va phu nd cdc co quan va md bao khac Qua trinh bdnh thudng thd hien loang mau, giam bach cdu, mach ddp chdm Su sinh san manh me cua cdu trung nidm mac rudt va su phd huy cdc td bao bidu md rudt ddn tdi hdu qua la trdn cdc vung protit bi chdt, hd vi khudn gdy mu se sinh san Cac loai vi khudn cdn lam nang them qua trinh vidm rudt, gdy rdi loan churc nang hd'p thu va vdn dong cua rudt, ddn den vdt ia chay Conway D.P, Mackenzie va Dayton (1999) cho bid't, chinh tdn thuong rudt cdu triing gdy da lam anh hudng ddn kha nang tang cua vdt nudi Gobzem V.P (1972) cho rdng, mat 10 - 15% nude co thd se lam cho vdt chdt Theo tac gia, su rdi loan trao doi nude se lam tang dinh cua mau, lam cho tim hoat ddng kho khan hon Williams R.B., Busshell A.C va cs (1996) cho thdy, qua trinh gdy benh cua cdu triing gidng Eimeria nhu sau: thur nha't d ruot, dudi tac ddng cua dich da day, dich rudt va dich mdt, Oocyst bi phd vd va giai phdng bao tu cdu trung (Sporocyst) Chung ldp turc chui vao cac te' bao bieu bi de ky sinh va hinh Schizont 1, giai phong cdc Merozoit Cdc Merozoit tiep tuc xdm nhdp vao te? bao mdi Ngay thii hai va ba, sau hoac the' he Schizont hoac Schizont 3, cac Merozoit the he cudi cung se phat trien biet hoa trd giao tu due, giao tu cai te' bao nidm mac ruot va tuye'n Luc nay, hien tupng xung huyd't nidm mac ruot la bieu hidn can ban, hidn tupng xud't huyet cdn it Ngay thur tu giao tir due kd't hop vdi giao tu cai hinh hop tu, rdi trd Oocyst Ngay thur nam hang loat te' bao nidm mac rudt bi phd vd, giai phong Oocyst vao xoang ruot, gdy hien tupng xud't huyet lan tran, te' bao bieu md long troc, lam cho ruot trd nen mong Den thur bdt dau xuat hien Oocyst thai qua phan Nhung xet nghidm mau ve hoa sinh va hinh thai cho thd'y, bi benh cdu trung, lupng hong cau va hemoglobin giam, vat bi thieu mau Ngoai ra, vao thdi kv benh tien trien cap tinh tha'y giam lupng dudng du tru mau, giam catalaza va lupng kiem du tru Do la nguyen nhan ddn de'n benh sue nhanh chdng kiet sue va chet (Kolapxki N.A va cs, 1980) 290 Gay benh c^u trung cho 1cm tren 30 tudi Thai Nguyen, Nguydn Thi Kim Lan va L i Minh (2006 - 2008) tha'y: thdi gian u benh la - ngay, thdi gian 1cm bat dau thai Oocyst la - ngay, sd lucmg Oocyst/gam phan cao nhat d 15 - 21 sau gay nhiem, giam d 22 - 27 va tir thur 28 trd di khong Oocyst phan 3.2 Trieu chumg benh cau trung lorn Trieu chung lam sang cua Ion bi benh cau trung thay doi thuoc vao tuoi vat, loai c3u trung, sd lugng Oocyst co mat timg co the 1cm - C) 1cm con: Theo Nguyen Due Luu va cs (2004), ty le Ion mac benh tir 50 - 70% Benh thudng xay d the ca'p tfnh hoac man tmh, ty le tir vong tir 10 - 20% neu khong dieu tri kip thdi Sau - ii benh, 1cm dot nhien u ru, met moi, hay nam, it bu va bo bu Sau khong lau chung ia chay manh, phan loang hoac nhay, mau tir vang den trang, mui kham va co lan mau (trong trudng hop nang mau chiem phan ldn phan) (Truong Van Dung va cs, 2002) Quan sat ky 1pm benh tha'y 1cm bi chudng hoi, day bung, kho chiu, non, mat nude va co hien tuomg dau bung, nam cong lung Ngoai ra, co co bieu hien than kinh nhu di khong vumg, di vo hudng hoac nam co giat Theo Nguyln Thi Kim Lan (2003), 1cm nhiem Isospora suis co the bi nhiem cac Rotavirus, gay benh Ion ia phan trang Dao Trong Dat va cs (1964) cho biet, ty le nhiem cau triing d 1cm la 7,29%, lorn ia phan trang la ,2 % Theo Biester H.E va Muray (1934), Ion bi nhiem E debliecki vdi sd lucmg Ion se gay ia chay kem an, sinh trudng kem va mot sd Ion bi chet Andrew va cs (1952) cho bie't, quan sat phan ciia Ion co trieu Oocyst loai E spinosa khong thay lpn co bieu hien trieu chung lam sang Nhung, bang thuc nghiem gay nhiem cho Ion 12.000 Oocyst E spinosa , Wiesenhiitter (1962) da thay trieu chung ia chay, sdt nhe d Ion Alicataz J.E va Wilier E.L (1946) cung cho r^ng: Ion nhiem 20 - 30 trieu Oocyst E debliecki gay Ion ia chay, giam an vao thu sau gay nhiem va chet sau 15 Gan day, Wiesnhiitter E va cs (1962), Wiesnhiitter E (1963) nghien cuu thay, lgn nhiem 10.000 E debliecki gay cho lgn ia chay, gay yeu Lam Thi Thu Huong (2002) da xet nghiem 128 lgn co trang thai phan long, thav ty le lgn nhiem Cryptosporidium la 37%, gay cho lgn tieu chay, coi coc, cham ldn, sue de khang giam 291 K^t qua nghidn curu cua Nguyen Thi Kim Lan v i cs (2006) cho th£y, ly le dd nhiftm cdu trung d Ion phan binh thudng v i phan long khac ro ret Ldn bi tieu chay co ty le nhiem cdu trung la 56,32% cao hon ty le nhiem cdu trung cua Ion co trang thai binh thudng (36,50%) Xet v6 mure dd nhiem, Ion bi tieu chay nhiem cau trimg nang hon nhieu so vdi Ion binh thudng - C) Ion trudng thanh: Ion choai va Ion trudng thanh, benh thudng th£ hien man tinh Lem gay rdc, khdng tang trong, nudi dudng kem cd the’ ia chay, va chi cd Ion mdi chet benh cdu trung Theo Pham Sy Lang va cs (2006), Ion mac benh d th£ man tinh, tinh them an thay d 6i khdng ldn, tdc dd suy ye'u co the cham Lon nai va Ion trudng bj nhiem cdu trung nhung khdng bi£u hien trieu chumg lam sdng, dd chung ld ngudn tang tri? va truydn ba mdm benh tu nhien 3.3 Benh tich cua Ion bi benh cdu trung Ki£m tra Ion chet cdu trung thudng thdy: xac chet gdy com, bdn, niem mac nhot nhat, trdng bech hoac xanh tai M6 kham Ion chet thdy benh tich tap trung chu ye'u d dudng rudt, dac biet d doan ta trang, dudi ta trang va rudt gia Benh tich thudng thdy la: - Trong ruot non chiia mot chat nude long, mau da cam vdi nhung soi chdt nhdy 7, - O ta trang va dudi ta trang: niem mac ruot bi viem tir cataz den xudt huyet va hoai tu Nao niem mac va kiem tra ruot ve mat md hoc, thdy bieu bi hdu nhu hoan toan duoc thay the' bdi mot ldp cdu trung - O ruot gia: ruot gia bi day len, niem mac ruot gia cd mang gia hoai tu, mang gia cd the thay phan - Cac hach mang treo ruot sung Ngoai ra, cung thdy nhung benh tich viem phoi, cd le ke phat cac vi khuan sinh mu gdy Theo Kolapxki N.A va cs (1980), mang niem mac ruot non viem cataz, bj benh keo dai co the bj viem xuat huyet khdng chi d ruot non ma ca d ruot gia Tai chd ruot bj viem thay nhung not to bang hat ke, xem kmh hien vi cac not thdy cd cac nang trung, cac the’ phan lap va the’ phdn doan Gay benh cau trung cho Ion roi mo kham Ion mdc benh, Nguyen Thj Kim Lan va Le Minh (2006 - 2008) cho biet, cdu trung ky sinh va gay benh tich d ruot non cua I o n , khong thay ky sinh va gay benh tich d ruot gia Lam tieu ban vi the, tac gia nhan thay nhung bien doi benh ly vi the’ d rudt non 1cm cau triing gay Nhung bien doi chii yeu duoc ghi la id cac hinh 140, 141, 142, 143, 144, 145 292 Hinh 141 Hinh 140 m6 ruot bi bong troc cung Hinh 142 Hinh 143 ' Long nhung u y - f.;- t _• Hinh 144 bi ton'^thucmg ' W » Hinh 145 MlfiN DICH HOC TRONG BENH CAU TRUNG 4.1 Nghien curu ve mi 6n dich eau trung d vat nuoi Tyzzer (1929) da chung minh bang thuc nghiem la co muc midn dich benl cau trung: Muc 1: phat sinh sau vat nhidm mot luong nho cau trung Khi se tao r; midn dich yeu va neu gay nhidm cho chung mot lieu cau trung cao hon (lieu sieu nhidm thi chiina se mac bdnh lai 29 Mure 2: vat nhidm mdt lupng lPn c£u trung Trong trudng hpp se co midn dich vit m ic bdnh lai Tac gia cho ring, cudng dd midn dich co lidn quan ddn sd lupng ciu trung xam nhap vao co thd Nhin dinh niy dupe Beyer xac nhan thi nghidm trdn tho, vi Paskin xac nhin thf nghidm trdn g i Bachman (1930) cho ring, midn dich theo tudi hinh thinh gia sue chung tai nhidm ciu trung nhidu lin Horton Smith (1963) cung chung minh didu do, tic gia nudi cich ly g i den thang tudi, khdng cho tidp xuc vPi ciu trung Sau thang tudi, cho nhidm tu nhidn thiy g i rit cam thu vdi E tenella, nhung sau nudi binh thudng thi g i khdng bi nhidm E tenella nua Wiesnhiiter E v i cs (1962) da cho gay nhidm thuc nghidm E debliecki, thiy lpn thai Oocyst til ngiy thur ddn ngiy thur 14, rdi khdng thiy thai Oocyst Sau - tuin lai cho nudt mot sd lupng ldn Oocyst ciu trung thi sd lupng Oocyst thai th ip hon lin thur nha't c o dupe tfnh midn dich viing chic, phai cho nudt Oocyst hang ngiy, ft nhit 100 ngiy Romel va cs (1970) da nghien cuu phan urng midn dich vdi E scabra thiy: huydt midn dich cd tac dung ngan can su nhidm Oocyst ciu trung nhung khdng thinh cdng lim Tuy vay, bang phuang phap dung hoa chat Parammethazone ace tat vi Dexamethazone cung da ngan can su nhidm ciu triing 4.2 T m h dac hieu cua m idn djch c iu tru n g Eimeria Tyzzer (1929) xac dinh ring: tfnh dac hieu cua midn dich ciu triing l i co that Sau gay nhidm cho g i bang E tenella (lin 1), tac gia tiep tuc gay nhidm lin cach tuin vdi loii ciu trung: E tenella, E maxima, E acervulina Khi md kham, dng chi phat hien tha'y benh tfch d rudt (nai gay benh cua ciu triing loii E maxima, E acervulina) ma khdng phat hien benh tfch a manh tring (noi gay benh cua ciu triing loai E tenella) Rose M.E (1962) da chung minh tfnh dac hieu theo loai rit nghiem ngat Eimeria bang phuang phap ket tua tren thach 4.3 C a che dap irng midn dich cau triing Theo ca che dap dng midn dich chung, muon co khang the phai co khang nguyen kfch thfch ca the’ Trong thuc tidn, sir sdng cua dong vat luon didn qua trinh tiep nhan khang nguyen nhung khdng phai tat ca ddu hinh khang the Midn dich cau triing Eimeria chi hinh co su hien dien cua cau triing Eimeria (Lillehoj, S.H., 1996) Ban chat cua dap ung midn dich bao gom: dap urng midn dich te bao va dap ung midn dich dich the (Nguydn Ngoc Lanh, 1982; Nguydn Nhu Thanh va cs 1997) * Midn dich te bao Theo Horton Smith va cs (1963), phan ung te bao bieu bi ruot tho vdi cau trung nhu sau: mot phan te bao bieu bi cuon vao ben trong, cach ly khoi ciu triing lam cho cac 294 giao tu cua cdu trung khd ket hap vdi Theo ong, cac Meroz rudt da kich thich su hinh khang th& , „ tnJC Nhicdnxki (,9 ) c«ng nhan dinh, co s6 mien dich cna * nud >a s« Nha xudt ban Ndng nghidp, Ha Ndi Lucmg Van Hua'n, Ld Huu Khucmg, 1997, K y sinh va benh ky sinh a gia sue gia cam Tap (Phan ddng vat chan ddt va nguydn bao), Vidn Dai hoc Qudc gia TP H6 Chi' Minh, Tr 383 386 Hunter Archie(2000), So tay dich benh dong vat, Nhzi xudt ban Ban d6, Ha Ndi (Pham Gia Ninh va Nguydn Dure Tam dich) Lam Thi Thu Hucmg, Ducmg Chi Mai va cs (2002), 'Tinh hinh nhiim C ryptosporidiu m tren heo tai mot so' trai va Id mo thuoc p h d Ho Chi Minh" , Tap chi' khoa hoc ky thuat thu y, Hdi thu y Vidt Nam, tap IX, (sd 2), Tr.47-52 Lam Thi Thu Hucmg (2004), "Tmh hinh nhidm mdt sd lo&i c iu trung ducmg rudt (Isospora, Eimeria va Cryptosporidium) heo mdt sd trai chan nudi tai phd H6 Chi' Minh", Tap chi' khoa hoc ky thuat thu y, Hdi thu y Vidt Nam, tap XI, (sd 1), Tr.26-32 Kolapxki N.A., Paskin P.I (1980), Benh cdu trung a gia sue gia cam, Nha xua't ban Ndng nghiep, Ha Noi (Nguydn Dinh Chi' va Trdn Xuan Tho dich) Nguydn Thj Kim Lan, Trdn Thu Nga (2005), "Mdt sd dac dilm dich td va vai tro cua cdu trung hdi chung tidu chay a Ion", Tap chi khoa hoc ky thuat thu y, H oi thu y V iet Nam, tap XII, (sd 4), tr.40-46 Nguydn Thi Kim Lan, Trdn Thu Nga (2005), "Tinh trang d nhidm cdu triing 1cm khu vuc chudng nudi va thdi gian phat trien cua Oocyst toi giai doan cam nhidm", Tap chi' khoa hoc ky thuat thu y, tap XII, so 5; tr 45-49 10 Nguyen Thj Kim Lan, Le Minh, Nguydn Thj Ngan (2006), "Vai trd cua ky sinh trung duang tieu hod hoi chung tieu chay a lan sau cai sua tai Thdi Nguyen", Tap chi' khoa hoc ky thuat thu y, Tap XIII, so 3, tr.36-40 11 Nguydn Thj Kim Lan, Le Minh (2008), “Xac djnh mot so loai cdu trung gav benh o Ion tai Thai Nguyen Su tdn tai, phat tridn cua Oocyst phan va nuoc thai chudng Ion”, Tap chi' khoa hoc ky thuat thu y, Tap XV, so 12 Nguydn Thj Kim Lan, Le Minh (2008), “S u p h a t trien vd khd ndng ton tai cua O ocxst cau trung lan ddt ”, Tap chi' khoa hoc ky thuat thu y, Tap XV, so 13 Nguydn Ngoc Lanh (1982), Tim hieu mien dich hoc (tap ), Nha xuat ban Y hoc Ha Noi 14 Pham Sy I.ang To Long Thanh (2006), Benh dem bao ky sinh a dong vdt nudi Nha xuat ban Ndng nghiep Ha Noi, Tr 138 - 142 302 15 Nguydn Due Luu, Nguydn Huu Vu (2004), M ot s o 'benh quan d lan , Nha xua't ban Van h6a thdng tin, Ha Ndi 16 Murray P.K (1997), ’Vacxin phdn tu-phdng ky sinh trung dong vat", Tap chi khoa hoc ky thuat thu y, Hdi thu y Vidt Nam, tap IV, (sd 4), Tr.88-94 (Bui Khanh Linh dich) 17 Ld Van Nam (2003), Benh cdu trung gia sue, gia cam, Nha xuat ban Ndng nghiep, Ha Noi 18 Hoang Thach va cs (1999), Khao sat tinh hinh nhidm c^u trung ga a phtf H6 Chi Minh, m6t stf vung phu can va thur nghiem m6t so thuoc phong tri Luan an tien si nong nghidp 19 Nguydn Nhu Thanh, Ld Thanh Hoa (1997), Mien dich hoc thu y, Nha xuat ban N6ng nghiep, Ha Ndi * Tieng Anh 20 Adams D.O and T.A Hamilton (1984), The cell biology o f m acrophage activation , Anu Rev Iminunol 2, P 283 21 Bachman G.W (1930), Immunity in experimental coccidiosis of rabbits, Amer, Hyg 12, P 641 22 Chae C (1998), Diarrhea in nursing piglets associated with coccidiosis, prevalence, microscopic lesions and coexisting microorganisms, Vet Rec, P 143, 417- 420 23 Ellis C.C (1986), "Studies of the viability of the Oocyst of Eimeria tenella, with particular reference to condition of incubation", Cornell Vet (28), P 267 24 Goodrich H.P (1994), Coccidian Oocyst, Parasitology, P 36- 72 25 Horton Smith C Brit Vet J (1963), "Immunity to avian coccidiosis", Coccidiosis, World poultry, P 99 - 106 26 Johannes Kaufmann (1996), Parasitic infections o f dom estic animal, Birkhauser Verlag, Berlin 27 Levine N.D (1985), Veterinary Protozoology, The Iowa Stale University Press Ames, Iowa, USA 28 Tyzzer E.E (1929), Coccidiosis in gallinaceous bird, Amer J Hyp, P 43 - 55 30 Chiu trach nhidm xuat ban NGUYfiN CAO DOANH Phu trach ban thao BICH HOA - HOAl ANH Trinh bay, bia THANH BINH NHA XUAT BAN NONG NGHlfcP 167/6 Phuang Mai - Ddng Da - Ha Ndi DT: (04) 5761075 - 8521940 Fax: 04.5760748 CHI NHANH NXB NONG NGHIEP 58 Nguydn Binh Khiem - Q1 - Tp Ho Chi Minh DT: (08) 8.299521 - 8.297157 Fax: 08.9101036 In 215 ban kho 19x27cm tai Xucmg in Nha xuat ban Nong nghiep Q uyet dinh in so 338-2007/CXB/69-57/NN Cuc Xuat ban cap thang nam 2007 In xong va nop luu chieu quv 1/2008 304 [...]... Hi N6i 23 Nguydn Van Tho (20 02) , "Moi giai truyen benh cua sdn Id rudt lan Fasciolopsis buski" Tap chi khoa hoc ky thuat thu y, tap IX, s6 4, Tr 38 - 42 24 Nguydn Van Tho (20 03), "Su phan tan va kha nang ph£t tri£n cua mOt s6 trung giun, sdn lpn qua hd thdng Biogas", Tap chf khoa hoc ky thuat thu y, tap X, s6 3, Tr 22 - 27 25 Nguydn Van Tho (20 05), Khao sat m6t sd dac di&n sinh hoc, dich tS hoc, bien... axit axetic 2% song dupe 9 ngay; trong axit axetic 3% sdng duoc 6 ngay; trong nude muoi NaCl 5% sdng dupe 3 gio; trong nude mudi NaCl 20 % bi diet sau 30 ngay (Nakagawa, 1 921 ) 1 52 2.4 Tap quan chan nuoi va dtftmg truyen benh Lon nhiem F buski nang hay nhe phu thuoc vao tap quan cho an thuc an song hay chin, thuc an xanh cho lgn tr6ng can hay dudi nuoc Lon an thuc an song ty le nhidm F buski cao ga'p 3... kha nang phat trien Vi vay, bien phap xu ly dan gian la xay 2 - 3 be’ chua nude thai trudc cira thai cua be’ Biogas de lam lang trumg, giu trung lai trong be ma khong cho phat tan ra ngoai canh - Ve sinh thu y, quan ly va nuoi dudng tot dan lgn Thudng xuyen coi trong cong tac ve sinh thu y, quan ly, nuoi dudng dan lgn; dinh ky taiy san cho lgn bang thudc Praziquantel; khong cho lgn an song rau muong... gay xua't huyet va san trudng thanh hut mau ky chu Lupng mau ma mdi san doat cua ky chu xap xi 0,5 ml/ngay Reid J F S (1973) cho bie't, cd su thay doi protein huyet thanh cua nhumg de bi nhiem san la Fasciola Su thay doi nay xay ra sdm, ngay trong giai doan san non di hanh Do la su tang Globulin va giam Albumin huyet thanh 136 Gay nhidm thuc nghidm san la Fasciola cho de va theo doi dien bien benh ly,... u theo phuong phap nhiet sinh hoc de diet trumg F buski Nguyen Van Tho (20 05) da de xuat bien phap phong benh san la ruot cho Ion nhu sau: - Dung thudc Praziquantel tay san la ruot: 1cm nuoi thit (3 - 6 thang tuoi) nen tay 1 lan vao thdi gian 2 - 2, 5 thang tuoi Lon sinh san m 6 i nam tay 2 lln, khoang cach giua 2 lan tly la 4,5 thang Lem trong thdi ky mang thai khong nen tly vi chua cd ket qua thuc... buski trirdng thanh thudng ky sinh d doan ta trang va khong trang cua lpn va ngudi, nhung ky sinh nhieu nhat d ta trang Trinh Van Thinh (1969), D 6 Hinh 61 F buski nhuom carmin Duong Thai (1973) cho biet, doi khi tha 'y san d da day va ruot gia 1 .2. 2 Chu ky sinh hoc Chu ky sinh hoc (vong phat tridn) cua F buski tuong tu cac san la khac thuoc ho Fasciolidae San trudng thanh sdng ky sinh d ruot non, de trung... Thi Lai (1996) "Mdt sd chi tieu sinh ly mau cua trau mac benh san la gan ", Tap chi' khoa hoc ky thuat thu y Tap III, s6 1, Tr 82- 86 17 Trinh Van Thinh (1963), Ky sinh trung thu y, Nha xua't ban nOng th6n, Ha NOi, Tr 28 1 29 2 18 Trinh Van Thinh, Dd Duang Thai (1978), Cong trinh nghien ciru ky sinh trung o'Viet Nam, Tap 2: Giun san a d6ng vat nudi, Nha xu£t ban khoa hoc va ky thuat, Ha NOi 19 Luang T6 Thu,... d vung doc hai ben sudn va doc xuong tic) Xuat hien thuy thung d mf mat, yem, nguc, bo phan sinh due Thuy thung ban dau luc thay, luc khdng, vd sau thay lidn tuc Con vat nhai lai yeu, khat nude, ia chay xen ke tao bon, gay yeu dan Giai doan sau di thao nhieu han va gay rat nhanh Kiem tra lam sang thay gan sung to va dau Cd the thay hien tugng xay thai d bd cai bi benh, lugng sua cd the giam 30 - 50%... d6ng bang sdng H6ng, Luan in Ti£n sy nOng nghidp, Trucmg DH ndng nghidp 1, Ha N6i 26 Phan The Vidt, NguySn Thi Ky, Nguydn Thi Le (1977), Giun sdn ky sinh a ddng vat Viit Nam, Nha xukt ban khoa hoc & ky thuat, Hi Ndi * Tieng A nh 27 Barlow C H (1 925 ), "The life cycle of the human intestinal fluke Fasciolopsis buski (Lankester)", Am J Hyg Monogr, (4), P 1 - 98 28 Dorothy J (1968), Parasites of man and... khuyen cao rang, tren thi truang Viet Nam hien 141 nay n6n su dung Fasinex - 900 dang vien (1 vi6n/75 - 100 kgTT) hoac Fasinex - 900 dang sua (10 mi/100 kgTT), cho hieu luc cao Sir dung Fasinex lidu 12 mg/kgTT t 4y san la gan cho trau, Phan Luc va Trdn Ngoc Thang (1999) cho biet, thuoc co hieu luc va hieu luc tay sach san deu dat 100% Nguyen Thi Kim Lan va cs (20 00) da thu nghiem mdt s6 thu 6c tay san ... Gidng Ceylonocotyle Namark, 1937 Loai Ceylonocotyle scoliocoelium (Fischoeder, 1901) Loai Ceylonocotyle dicranocoelium (Fischoeder, 1901) Loai Ceylonocotyle dinniki (Eduarko, 19 82) Gidng Cotylophoron... lai be ma khong cho phat tan ngoai canh - Ve sinh thu y, quan ly va nuoi dudng tot dan lgn Thudng xuyen coi cong tac ve sinh thu y, quan ly, nuoi dudng dan lgn; dinh ky taiy san cho lgn bang thudc... Paramphistomum iclukawai (Fukui, 1 922 ) 159 Loai Paramphistomum liorchis (Fukui, 1 922 ) Gidng Gigantocotyle Nasmark, 1937 Loai Gigantocotyle bathycotyl (Fischoeder, 1901) Loai Gigantocotyle Goldberger, 1910)